Clinical Pathology & Research Journal最新文献

Tamoxifen Induced Down Regulation of mTOR and UP Regulation of Exosomal miR-99b-5p and miR-100-5p in mcf-7 Breast Cancer Cells 他莫昔芬诱导mcf-7乳腺癌细胞mTOR下调，外泌体miR-99b-5p和miR-100-5p上调

Clinical Pathology & Research Journal Pub Date : 2024-01-01 DOI: 10.23880/cprj-16000193

Ghazale Faraji

{"title":"Tamoxifen Induced Down Regulation of mTOR and UP Regulation of Exosomal miR-99b-5p and miR-100-5p in mcf-7 Breast Cancer Cells","authors":"Ghazale Faraji","doi":"10.23880/cprj-16000193","DOIUrl":"https://doi.org/10.23880/cprj-16000193","url":null,"abstract":"Background: Breast cancer treatment has always been an important challenge in medicine. Recent studies have revealed the importance of microRNAs miR-99b-5p and miR-100-5p in breast cancer treatment. Moreover, however not fully understood, the impact of exosomal microRNAs in human cancer progress or inhibition has been highlighted yet. The present study aimed to investigate the effects of one of the most prescribed drugs in breast cancer chemotherapy, tamoxifen, on expression level of miR-99b- 5p, miR-100-5p and also mTOR gene in MCF-7 cell lines. Methods: MCF-7 cells were treated with 30 µM tamoxifen while being in a 70% surface density, then the expression level of mentioned microRNAs and mTOR gene were investigated in RNA extracts of the cells and their exosomes. Results: Results indicated the presence of a significant increase (P <0.01) in both miR-99b-5p and miR- 100-5p expression levels in cell lines following tamoxifen treatment. Also, the evaluation of mTOR gene expression in the same cells after treatment with tamoxifen showed significant (P <0.05) expression reduction. Conclusions: Interestingly, the decrease in mTOR expression level was completely consistent with increase in the expression of exosomal microRNAs level, which seems to be reasonable considering the targeting of the mTOR gene by the two mentioned microRNAs. The results of this study suggest that the therapeutic effects of tamoxifen on breast cancer cells, can be justified not only by its direct effect on cancer cells, but also by indirect impact on elements like microRNAs expression.","PeriodicalId":330153,"journal":{"name":"Clinical Pathology & Research Journal","volume":"8 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.23880/cprj-16000193","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147895266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Diagnostic Accuracy of Periodic Schiff-Methenamine Silver and Masson’s Trichrome Stains for Immune Complex Deposits in Lupus Nephritis 周期性希夫-甲基苯丙胺银和马松三色染色对狼疮肾炎免疫复合物沉积物的诊断准确性

Clinical Pathology & Research Journal Pub Date : 1900-01-01 DOI: 10.23880/cprj-16000146

Bheemanathi Hanuman Srinivas

引用次数: 0

An Extremely Rare Case of Primary Ovarian Teratoid Carcinosarcoma: Cytological, Pathological and Immunohistochemical Findings 一例极罕见的原发性卵巢畸胎瘤肉瘤:细胞学、病理及免疫组化结果

Clinical Pathology & Research Journal Pub Date : 1900-01-01 DOI: 10.23880/cprj-16000144

K. Niwa

引用次数: 0

Automated Localization and Segmentation of Mononuclear Cell Aggregates in Kidney Histological Images Using Deep Learning 基于深度学习的肾脏组织图像中单个核细胞聚集体的自动定位和分割

Clinical Pathology & Research Journal Pub Date : 1900-01-01 DOI: 10.23880/cprj-16000140

D. Hadley

引用次数: 0

False Positive Conventional Cytogenetic Findings that Mimic the Recurrent Chromosome Abnormalities Associated with Hematolymphoid Disorders 假阳性常规细胞遗传学结果模拟与淋巴系统疾病相关的复发性染色体异常

Clinical Pathology & Research Journal Pub Date : 1900-01-01 DOI: 10.23880/cprj-16000148

Shivani Golem

引用次数: 0

Genetic Evolution of the Novel Coronavirus 新型冠状病毒的遗传进化

Clinical Pathology & Research Journal Pub Date : 1900-01-01 DOI: 10.23880/cprj-16000143

S. Bhattacharyya

引用次数: 0

Neuroendocrine Tumor of Cecum-Well Differentiated Intermediate Grade: G2 Presented with Aggressive Biologic Behavior: Case Report with Review 盲肠高分化中级神经内分泌肿瘤:G2表现为侵袭性生物学行为:病例报告并复习

Clinical Pathology & Research Journal Pub Date : 1900-01-01 DOI: 10.23880/cprj-16000150

S. Jagtap

{"title":"Neuroendocrine Tumor of Cecum-Well Differentiated Intermediate Grade: G2 Presented with Aggressive Biologic Behavior: Case Report with Review","authors":"S. Jagtap","doi":"10.23880/cprj-16000150","DOIUrl":"https://doi.org/10.23880/cprj-16000150","url":null,"abstract":"Neuroendocrine tumors (NETs) are relatively rare neoplasms of the gastrointestinal tract with variable clinical presentations. A 53 year old female presented with a chief complaint of intermittent right-sided abdominal pain, nausea, loose stools of and on of 8 month duration. The computed tomography of abdomen and pelvis, both plain and with contrast revealed the presence of a large well defined homogenously enhancing polypoidal mass lesion noted in the caecum measuring 2.8 X 2.6 X 2.8 cm in size. On colonoscopy revealed the presence of a polypiodal mass in the cecum measuring 3 cm with mucosal ulceration. The biopsy reported as suggestive of neuroendocrine tumor. The wide surgical resection of cecal mass with lymph node dissection was done. On histopatholgy reported as well differentiated neuroendocrine tumor, intermediate grade G2 of cecum with mesenteric lymph node metastasis. On clinical of stage III. The tumor was strong and diffusely positive for expression of synaptophysin and chromogranin A. The expression of the cell-cycle-associated Ki-67 antigen by MIB-1 monoclonal antibody was 3%. Herewith we are presenting rare case of well differentiated neuroendocrine tumor with intermediate grade or grade 2 of cecum with aggressive biologic behavior.","PeriodicalId":330153,"journal":{"name":"Clinical Pathology & Research Journal","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120966373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenic Microorganisms in Fish: A Cause for Concern 鱼类中的病原微生物:引起关注的原因

Clinical Pathology & Research Journal Pub Date : 1900-01-01 DOI: 10.23880/cprj-16000134

S. Bhattacharyya

引用次数: 0

Clinical Pathology (CP) or Laboratory Medicine (LM): What is there in the Name? 临床病理学(CP)或检验医学(LM):名称中有什么含义?

Clinical Pathology & Research Journal Pub Date : 1900-01-01 DOI: 10.23880/cprj-16000133

Ashok Kumar Mukhopadhyay

引用次数: 0

Brief comparison of the Current Coronavirus COVID-19 Pandemic (SARS CoV 2) and the Spanish Flu Epidemic 当前冠状病毒COVID-19大流行(SARS CoV 2)和西班牙流感流行的简要比较

Clinical Pathology & Research Journal Pub Date : 1900-01-01 DOI: 10.23880/cprj-16000138

引用次数: 1