Rain in PluralPub Date : 2002-06-01DOI: 10.2307/j.ctvz93931.7
Fetter Ms
{"title":"A MATTER OF TIME","authors":"Fetter Ms","doi":"10.2307/j.ctvz93931.7","DOIUrl":"https://doi.org/10.2307/j.ctvz93931.7","url":null,"abstract":"","PeriodicalId":329437,"journal":{"name":"Rain in Plural","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132974195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rain in PluralPub Date : 1981-12-31DOI: 10.2307/j.ctvb1hrbz.22
Jin-hui Yuan, R. Zhang, R. Zhang, Li-Xia Guo, Xing-wang Wang, Dan Luo, Yong Xie, H. Xie
{"title":"P","authors":"Jin-hui Yuan, R. Zhang, R. Zhang, Li-Xia Guo, Xing-wang Wang, Dan Luo, Yong Xie, H. Xie","doi":"10.2307/j.ctvb1hrbz.22","DOIUrl":"https://doi.org/10.2307/j.ctvb1hrbz.22","url":null,"abstract":"AIM To investigate the effects of taxol on SMMC-7721 human hepatoma and its mechanisms. METHODS In vitro cell growth was assessed by trypan blue exclusion method. Experimental hepatoma model was established by seeding SMMC-7721 cells subcutaneously into Balb/c(nu/nu) nude mice. In vivo tumor growth was determined by measurement of tumor diameter with Vernier calipers. The syntheses of DNA, RNA and protein were analyzed by incorporation of 3H-thymidine, 3H-uridine and 3H-leucine respectively. Using light and electron microscopes to observe the morphological changes of cells including mitosis and apoptosis. RESULTS Taxol was effective against SMMC-7721 human hepatoma cell growth in the ranges of 2.5nmol/L10nmol/Lwith mitotic arrest and apoptosis in vitro. DNA, RNA and protein syntheses in cells were also obviously suppressed by in vitro treatment of taxol for 72h. Taxol at 2.5nmol/L reduced 3H-thymidine uptake to about 34% of the control value (P£1⁄40.05). Increasing the dose of taxol to 20nmol /L resulted in a greater decrease in 3H-thymidine incorporation to 60% of the control value (P£1⁄40.01). At a concentration of 20nmol/L, the 3H-uridine and 3H-leucine uptakes were reduced to 52% (P£1⁄40.05) and 63% (P£1⁄40.01), respectively. In vivo, taxol significantly inhibited SMMC-7721 tumor growth at 10mg/kg, i.p. once daily for 10d. A more than 90% decrease in tumor volume was observed by day 11 (P£1⁄40.01) similarly with mitotic arrest and cell apoptosis. CONCLUSION Taxol has a marked anticancer activity in SMMC-7721 human hepatoma both in vitro and in nude mice. Its mechanisms might be associated with mitotic arrest, subsequently, apoptosis of the hepatoma cells. No obvious toxicity was observed with in vivo administration of taxol.","PeriodicalId":329437,"journal":{"name":"Rain in Plural","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1981-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125585266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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