Journal of Saidu Medical College, Swat最新文献_第3页

Dose Dependent Proliferation of Testicular Tissue Subsequent to Exogenous Neurokinin B Administration in Adult New Zealand White Rabbits 给成年新西兰白兔注射外源性神经激肽 B 后睾丸组织的剂量依赖性增殖

Journal of Saidu Medical College, Swat Pub Date : 2024-03-08 DOI: 10.52206/jsmc.2024.14.1.866

Faiqah Ramzan, Sundas Gul, Muhammad Haris Ramzan

{"title":"Dose Dependent Proliferation of Testicular Tissue Subsequent to Exogenous Neurokinin B Administration in Adult New Zealand White Rabbits","authors":"Faiqah Ramzan, Sundas Gul, Muhammad Haris Ramzan","doi":"10.52206/jsmc.2024.14.1.866","DOIUrl":"https://doi.org/10.52206/jsmc.2024.14.1.866","url":null,"abstract":"Background: Neurokinin B is a regulator of puberty and reproduction, however its chronic effects on gonads in adult male mammals remain elusive. Objectives: To investigate potentials of variable doses of Neurokinin B on histomorphology of testicular tissue and serum testosterone levels in adult rabbits. Material and Methods: This Laboratory based experimental study was conducted at Gomal Centre of Biochemistry and Biotechnology, Gomal University, Dera Ismail Khan, Pakistan from June-2019 to June-2020. Adult male New Zealand white rabbits (n=18) with an average weight of 1.52kg were randomly assigned to three groups. Study animals were administered subcutaneously with Neurokinin B in two variable doses: 1 µg and 1 çg. Control rabbits were injected with distilled water. After 12 days of continuous peptide treatment, animals (n=06) in each group were sacrificed. Testicular tissues were dissected and processed for light microscopy. Blood collection was performed directly from left ventricle, serum was separated and assayed for testosterone by enzyme linked immunosorbent assay.Results: Neurokinin B did not alter the body weight of experimental rabbits as compared to control. No significant change on testicular weight, testicular volume and gonadosomatic index was observed at any dose of Neurokinin B. Seminiferous tubular diameter (µm) increased non-significantly while seminiferous epithelial height (µm) increased significantly in high dose group as compared to control group. Serum testosterone concentration increased significantly (P?0.01) at both low (1çg) and high (1µg) doses of Neurokinin B as compared to control.Conclusion: Continuous administration of Neurokinin B effect positively on serum testosterone and testicular morphology.Keywords: Histomorphology, Neurokinin B, Tachykinins, Testes, Testosterone","PeriodicalId":326561,"journal":{"name":"Journal of Saidu Medical College, Swat","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140257512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meaning and Statistical Analysis of T-Tests in SPSS SPSS 中 T 检验的含义和统计分析

Journal of Saidu Medical College, Swat Pub Date : 2024-03-08 DOI: 10.52206/jsmc.2024.14.1.889

Muzammil Ahmad Shah

引用次数: 0

Effectiveness of Phloroglucinol in Shortening the Active Phase of the First Stage of Labour in Term Pregnancies 氯葡萄糖醇对缩短足月妊娠第一产程活跃期的效果

Journal of Saidu Medical College, Swat Pub Date : 2024-03-08 DOI: 10.52206/jsmc.2024.14.1.844

Samreen, Bushra Rauf, Laila Nazir, Kiran Jehangir, Nasreen Kishwar

{"title":"Effectiveness of Phloroglucinol in Shortening the Active Phase of the First Stage of Labour in Term Pregnancies","authors":"Samreen, Bushra Rauf, Laila Nazir, Kiran Jehangir, Nasreen Kishwar","doi":"10.52206/jsmc.2024.14.1.844","DOIUrl":"https://doi.org/10.52206/jsmc.2024.14.1.844","url":null,"abstract":"Background: Labour is a normal physiological process, involving increasing myometrical contractions, cervical dilation and effacement resulting in delivery of the fetus and placenta and the initiation of the process of lactation. First Stage of labour lasts for 12-16hrs in primigravida and 6-8 hrs in multigravida. Two important characteristics of labour are uterine contractions and cervical effacementObjectives: To determine the effectiveness of phloroglucinol in shortening the 1st stage of labour in term pregnancies.Material and Methods: It was a double blinded randomized control trial (RCT) conducted in Obs and Gynae department of Hayatabad Medical Complex, MTI, Peshawar Pakistan, over a period of 6 months from 20th October 2022 to 20th April 2023. Patients were selected randomly for placebo and pluroghlucinol group and recorded on structured proforma. Total 375 women were selected in first stage of labour. In which 187 were given Phloroglucinol and 188 women received placebo. Results: The mean age of patients in phloroglucinol group was 28.3 ± 4.01 and in control group was 26.6 ± 6.8 years. The mean period of gestation in Phloroglucinol group was 37.3 ± 4.0 while in control group was 38.6 ± 2.08 weeks. The mean BMI of patients in Phl group was 22.12 ± 1.4 while in control group was 21.6 2 ± 1.5. The mean duration of active phase of 1st stage of labour in Phl group was 290.4 ± 29.7 minutes while in control group was 402.4 ± 29.7 minutes. Conclusion: Phloroglucinolhas significant effect in shortening the 1st stage of labour.Keywords: Active phase, Labour, Phloroglucinol, Pregnancy.","PeriodicalId":326561,"journal":{"name":"Journal of Saidu Medical College, Swat","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140258157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Journey and Experience of an Editor 编辑的历程与经验

Journal of Saidu Medical College, Swat Pub Date : 2024-03-08 DOI: 10.52206/jsmc.2024.14.1.877

Sahibzada Saeed Jan

{"title":"Journey and Experience of an Editor","authors":"Sahibzada Saeed Jan","doi":"10.52206/jsmc.2024.14.1.877","DOIUrl":"https://doi.org/10.52206/jsmc.2024.14.1.877","url":null,"abstract":"I had been a member of the editorial board of the Journal of Saidu Medical College (JSMC) for the last 7 to 8 years. Looking back through this period, i grade it a pleasant experience. When I joined the team of JSMC, the journal was passing through its childhood to adulthood period. The hard work of registering it with Provincial Information Department and having the International Standard Serial Number (ISSN) number, already done as well as provisional recognition with Pakistan Medical & Dental Council (PMDC) was also accomplished. Yet a lot was still ahead and required as is usual with the scientific journals. I would like to appreciate the hard work of the pioneers and the struggle and commitment of the present team members, whose commitment has nurtured this young sibling to a fruitful tree. This journal is now indexed and registered with different national agencies like, Higher Education Commission, Pakistan category 'Y', College of Physician & Surgeon of Pakistan, Pakistan Scientific & Technological Information Centre, PakMediNet.com, and also has gained recognition at international level like; Member of Crossref to assign Digital Object Identifier (DOI) to each article, Following Creative Commons license for international acceptability, giving internationally recognized reviewer credits via Reviewer Credits.com, member of Eastern Mediterranean Association of Medical Editors (EMAME) and Index Medicus Eastern Mediterranean Region (IMEMR).","PeriodicalId":326561,"journal":{"name":"Journal of Saidu Medical College, Swat","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140257084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Pre-eclampsia in Women with Advanced Maternal Age 高龄产妇与先兆子痫的关系

Journal of Saidu Medical College, Swat Pub Date : 2024-03-08 DOI: 10.52206/jsmc.2024.14.1.822

Zoya Naeem, Seema Gul, Atikka Masud, Faiza Abrar, Dureshahwar, Zainab Tahir

{"title":"Association of Pre-eclampsia in Women with Advanced Maternal Age","authors":"Zoya Naeem, Seema Gul, Atikka Masud, Faiza Abrar, Dureshahwar, Zainab Tahir","doi":"10.52206/jsmc.2024.14.1.822","DOIUrl":"https://doi.org/10.52206/jsmc.2024.14.1.822","url":null,"abstract":"Background: Preeclampsia: Preeclampsia is a serious condition that typically occurs after the 20th week of pregnancy. Preeclampsia can occur in women of all ages, but the risk increases with certain factors. Objectives: This study aimed to find out the association of pre eclampsia in women with advanced maternal age. Material & Methods: This cross-sectional study was carried out in high dependency unit (HDU) of the Obstetrics and Gynecology Department of the Fauji Foundation Hospital, Rawalpindi, from 30th November 2021 to 29th May 2022. Total of 260 pregnant patients were selected through non-probability, sequential sampling. After taking written informed consent, information on the mother's age (measured in categories) and parity for each patient was gathered on a pre-made Performa. SPSS version 20 software was used to analyze the data. With a p-value of 0.05 considered significant, the chi-square test was employed to compare the maternal age to pre-eclampsia.Results: The mean age of the participants was 27.9± 6.02 years. Pre-eclampsia in pregnant women was discovered in 32 (12.3%) individuals. It was found in 21% of patients having aged <20 years, in 9.0% of patients having age 20-25 years, in 5.4% patients having age 26-30 years, in 14.2% patients having age 31-35 years and 27.5% % in patients having age >35 years and the results were statistically significant.Conclusion: The current study's conclusions indicate that pre-eclampsia affects pregnant women quite frequently and that advance maternal age is a risk factor for the disease.Keywords: Advanced maternal age, Pre-eclampsia, Pregnancy.","PeriodicalId":326561,"journal":{"name":"Journal of Saidu Medical College, Swat","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140257860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors Affecting the Birth Outcome Among Women with Eclampsia at Mardan Medical Complex, Mardan, Pakistan. 影响巴基斯坦马尔丹医疗中心子痫妇女分娩结果的因素。

Journal of Saidu Medical College, Swat Pub Date : 2023-12-19 DOI: 10.52206/jsmc.2023.13.4.827

Naila, Seema Gul, Fatima Rehman, Iffat Irshad, Nuzhat Amin, Nasim Akhtar, Sami Ullah

{"title":"Factors Affecting the Birth Outcome Among Women with Eclampsia at Mardan Medical Complex, Mardan, Pakistan.","authors":"Naila, Seema Gul, Fatima Rehman, Iffat Irshad, Nuzhat Amin, Nasim Akhtar, Sami Ullah","doi":"10.52206/jsmc.2023.13.4.827","DOIUrl":"https://doi.org/10.52206/jsmc.2023.13.4.827","url":null,"abstract":"Background: Eclampsia is a life-threatening complication of pregnancy. Identifying the factors affecting birth outcome in eclamptic patients allows for targeted interventions to enhance maternal and fetal well-being.Objectives: To identify the risk factors that adversely affect birth outcomes among women with eclampsia.Material and Methods: This Cross-Sectional Descriptive study was conducted at Gynae Unit in Mardan Medical Complex, Mardan, from 1st January to 31st Dec 2018. A total of 157 antenatal patients who presented with eclampsia and live fetuses were enrolled for the study through non probability sampling technique. The data on their demographic variables, gestational age at presentation, mode of delivery, and birth outcome were recorded.The logistic regression model was used to investigate the dependence of birth outcome on maternal age, gestational age, mode of delivery, Parity and Booking Status. Results: Our analyses indicate that maternal age, mode of delivery, and parity significantly affect the birth outcome in women suffering from eclampsia. These three factors had a significantly negative impact on the birth outcome as their estimates are less than 1 at 5% level of significance indicating the probability of live birth decreases for the Normal Vaginal Delivery, Parity (multigravida), and advanced maternal age.Conclusion: Increased maternal age, normal vaginal delivery and multiparity increased the probability of still births in patients with eclampsia. Compared to the Cesarean section mode, normal vaginal delivery had a lower probability of live birth. Multigravida had a lower probability of live birth in eclampsia patients. Keywords: Cesarean Section, Eclampsia, Live birth, Normal vaginal delivery, Stillbirth.","PeriodicalId":326561,"journal":{"name":"Journal of Saidu Medical College, Swat","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138960493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sleep Pattern in Undergraduate Dental Students Undergoing Clinical Summative Assessment in a Public Sector Dental Institute of Peshawar 白沙瓦公立牙科学院接受临床总结性评估的牙科本科生的睡眠模式

Journal of Saidu Medical College, Swat Pub Date : 2023-12-19 DOI: 10.52206/jsmc.2023.13.4.799

Iqbal Haider, Nayab Munib, Muhammad Tariq Khan, Suleman Elahi Malik

{"title":"Sleep Pattern in Undergraduate Dental Students Undergoing Clinical Summative Assessment in a Public Sector Dental Institute of Peshawar","authors":"Iqbal Haider, Nayab Munib, Muhammad Tariq Khan, Suleman Elahi Malik","doi":"10.52206/jsmc.2023.13.4.799","DOIUrl":"https://doi.org/10.52206/jsmc.2023.13.4.799","url":null,"abstract":"Background: Sleep is one of the most important determinants of human health. The quality of sleep has significant effects on academic performance and is influenced by multiple factors.Objectives: To determine the association between sleep patterns / Quality and academic performance of dental undergraduates during their summative clinical examination by using an objective measure.Material and Methods: This descriptive cross-sectional study was conducted at the Department of Medicine, Khyber Teaching Hospital Peshawar, from February 1, 2023, to June 30, 2023. A sample size of 82 was calculated by the Open Epi calculator. A validated questionnaire of the Pittsburgh Sleep Quality Index (PSQI) was used in this study. The study participants were selected by convenience based consecutive sampling technique and were 3rd BDS Students appearing in annual General Medicine Examination. After data collection, these variables were analyzed by SPSS version 26 software.Results: This study comprised 43(52.4%) female and 39(47.6%) male participants, and amongst them, 34.1% of females and 20.7% of males rendered their sleep quality bad, whereas 18.3% of females and 26.8% of males responded otherwise. The findings were positively associated with bad sleep quality (47.6%). Measures of sleep quality were significantly deranged among poor sleepers compared to normal sleepers. On average, the academic performance of poor-quality sleepers was good compared to good-quality sleepers. Still, this finding is not statistically significant, as evidenced by the t-test (Mean 574 ± SD 62.156 vs. 581 ± 79.769) and p-value of 0.640.Conclusion: Poor sleep quality was common among our students. The academic performance of these students was not statistically significant in comparison to that of good quality sleepers. Keywords: Academic performance, Dental undergraduates, PSQI, Sleep quality","PeriodicalId":326561,"journal":{"name":"Journal of Saidu Medical College, Swat","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138962289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maternal Outcomes in Placenta Accreta Spectrum: Experience at Lady Reading Hospital Peshawar, Pakistan 胎盘早剥谱的产妇预后：巴基斯坦白沙瓦雷丁夫人医院的经验

Journal of Saidu Medical College, Swat Pub Date : 2023-12-19 DOI: 10.52206/jsmc.2023.13.4.789

Wajeeha Syed, Nazia Liaqat, Tanveer Shafqat, Hina Farrukh

{"title":"Maternal Outcomes in Placenta Accreta Spectrum: Experience at Lady Reading Hospital Peshawar, Pakistan","authors":"Wajeeha Syed, Nazia Liaqat, Tanveer Shafqat, Hina Farrukh","doi":"10.52206/jsmc.2023.13.4.789","DOIUrl":"https://doi.org/10.52206/jsmc.2023.13.4.789","url":null,"abstract":"Background: Placenta accrete spectrum includes placenta accreta, increta and percreta and results in failure of placental separation from uterine wall after delivery. It can cause massive hemorrhage and is one of the major cause of maternal mortality.Objectives: To determine maternal outcomes in placenta accreta spectrum (PAS) at Lady Reading hospital PeshawarMaterial and Methods: This cross-sectional study was carried in OBGYN department of Lady Reading hospital Peshawar from January 1st 2022 till 31st December2022. Patients with placenta accreta spectrum were included for study. These cases were recruited from antenatal OPD and from obstetric emergency unit. Mode of delivery in all cases was cesarean section. All included patients were followed till discharge to determine maternal and fetal outcomes. Information was gathered on pre designed proformas.Results: The mean age of sample of 35 cases was 33 +-3.5 years. Mean gestational age at delivery was 34+-1.01. Sixty eight percent cases of placenta percreta spectrum had emergency cesarean delivery. Thirty four percent cases received multidisciplinary intra operative care. Hemorrhage needing blood and blood products transfusion was the commonest intra operative complication accounting for 68.6% cases. Surgical wound infection was the commonest post operative complication occurring in 31.4% cases. Two mothers died during the study period.Conclusion: Hemorrhage is the most common complication of placenta percreta spectrum.Keywords: placenta percreta, obstetric hemorrhage, surgical wound infection","PeriodicalId":326561,"journal":{"name":"Journal of Saidu Medical College, Swat","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138959304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stromal CD10 Expression in Invasive Breast Carcinoma in Correlation with ER, PR and Her2 Status and Tumor Grade. 浸润性乳腺癌基质 CD10 表达与 ER、PR 和 Her2 状态及肿瘤分级的关系

Journal of Saidu Medical College, Swat Pub Date : 2023-12-19 DOI: 10.52206/jsmc.2023.13.4.801

Faryal Javaid, Ayesha Sajjad, Shifa Basharat, Ayesha Safdar, Maria Tasneem Khattak, Maria Khan, Iqbal Mohammad Khan

{"title":"Stromal CD10 Expression in Invasive Breast Carcinoma in Correlation with ER, PR and Her2 Status and Tumor Grade.","authors":"Faryal Javaid, Ayesha Sajjad, Shifa Basharat, Ayesha Safdar, Maria Tasneem Khattak, Maria Khan, Iqbal Mohammad Khan","doi":"10.52206/jsmc.2023.13.4.801","DOIUrl":"https://doi.org/10.52206/jsmc.2023.13.4.801","url":null,"abstract":"Background: Increasing prevalence of breast cancer in our population is a leading cause of morbidity and mortality. Association of the tumor markers with the behavior of tumor cells may pave a way to better outcomes. Objectives: To investigate the frequency of Stromal CD10 expression in invasive breast carcinoma in correlation with ER, PR and Her2 status and tumor grade.Material and Methods: A total of 102 diagnosed cases of breast carcinoma who underwent mastectomies were examined. Immunohistochemistry was performed for Cluster of differentiation 10 (CD10), Estrogen receptor(ER) Progesterone receptor (PR) and Human epidermal receptor factor 2(HER2)for all the samples. Demographic characteristics and immunopathological variables were recorded. Results: Mean age of the participants was 54.05 ± 8.58 years. Immunohistochemical analysis revealed 83 (81.3%) cases as CD10 positive out of which 15 (14.7%) specimens were weak positive and 68 (66.6%) were strong positive. The expression of CD10 was significantly greater for patients with higher tumor grades (p-value 0.014). Significant association of stromal CD10 expression and ER negativity was observed (p-value < 0.01). Out of 83 (81.3%) specimens that revealed positive CD10 expression, 64 (62.7%) were found HER2 positive and 19(18.6%) were HER2 negative signifying a positive correlation with p-value of <0.01. Conclusion: Frequency of stromal CD10 expression is significantly associated with increasing tumor grade, HER2 positivity and ER negativity. It employs a prognostic role in predicting the aggressiveness of tumor as indicated by higher tumor grade and preemptive therapy with anti CD10 antibody may be beneficial in such candidates.Keywords: Breast carcinoma, CD10, ER, PR, HER2.","PeriodicalId":326561,"journal":{"name":"Journal of Saidu Medical College, Swat","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138960643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Characterization of Wild-Type Measles Viruses Isolated in Peshawar Pakistan 巴基斯坦白沙瓦分离到的野生型麻疹病毒的遗传特征

Journal of Saidu Medical College, Swat Pub Date : 2023-12-19 DOI: 10.52206/jsmc.2023.13.4.796

Zubeda Irshad, Zainab Irshad Khan

{"title":"Genetic Characterization of Wild-Type Measles Viruses Isolated in Peshawar Pakistan","authors":"Zubeda Irshad, Zainab Irshad Khan","doi":"10.52206/jsmc.2023.13.4.796","DOIUrl":"https://doi.org/10.52206/jsmc.2023.13.4.796","url":null,"abstract":"Background: Measles is a highly contagious disease, caused by the Measles Virus (MV). Knowledge of genotypes prevalent in any region is important in effective measles surveillance; by mapping distribution of prevalent MV genotypes at different points of time, in a region, gives an idea about the success of measles control through vaccination programs, by documenting the interruption of transmission of the endemic viral genotype. Objectives: To detect genotypes of wild type measles virus isolated from the samples collected of measles patients in Peshawar valley of Khyber Pakhtunkhwa (KPK) province, Pakistan.Material and Methods: This was a cross-sectional descriptive study. Nasopharyngeal and/or throat swab samples were collected during a measles outbreak, from February to March 2014, from clinically diagnosed measles patients (cases who met the clinical case definition of WHO). Collection and processing were done according to the standard protocols. RNA (Ribo-Nucleic Acid) was extracted directly from samples; cDNA (Complimentary Deoxy-Ribonucleic Acid) was synthesized, amplification and sequencing of 528bp of carboxy-terminal of N gene was carried out. Sequences were analyzed and data were compared with the standard reference strains of measles virus.Results: A total of nine sequences obtained were of good quality and enough in length to meet WHO criteria for genotype assignment. It was found that all the nine strains of measles virus belonged to clade B, genotype B3 and cluster B3.1. The rest of the 13 sequences which were shorter in length for genotype assignment also showed close resemblance to Genotype B3.Conclusion: Wild type measles virus strains, isolated from the samples of measles patients, in Peshawar valley of Khyber Pakhtunkhwa province of Pakistan were found to be genotype B3. It shows that genotype B3 may be the predominate genotype prevalent in Peshawar region at the time of this study.Keywords: B3 genotype, Genotyping of measles, Measles Virus, Molecular epidemiology.","PeriodicalId":326561,"journal":{"name":"Journal of Saidu Medical College, Swat","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138963164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0