Genetics Applications最新文献

Highly Sensitive Detection of DNA Fragments by an Inexpensive and Simple Method Fabricated In-House Blue LED Transilluminator 高灵敏度检测DNA片段的廉价和简单的方法自制蓝色LED透光

Genetics Applications Pub Date : 2023-11-10 DOI: 10.31383/ga.vol7iss2ga01

Ryosuke Ishii

引用次数: 0

Book of Abstracts - 3rd Congress of Geneticists in Bosnia and Herzegovina with International Participation - CONGUB&H 摘要书-第三届遗传学家大会在波斯尼亚和黑塞哥维那与国际参与- CONGUB&H

Genetics Applications Pub Date : 2023-09-29 DOI: 10.31383/ga.speced04

None GENUBIH

引用次数: 0

Book of Abstracts - International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH 摘要书-生物化学家和分子生物学家在波斯尼亚和黑塞哥维那国际会议- ABMBBIH

Genetics Applications Pub Date : 2023-05-18 DOI: 10.31383/ga.speced03

None 18.05.2023.

引用次数: 0

Effects of Silymarin on Blood Glucose Concentration, Hepatic Histopathological Changes and FOXA2 and FOXA3 Gene Expression in Streptozotocin-Induced Diabetic Male Wistar Rats 水飞蓟素对链脲佐菌素诱导的糖尿病雄性Wistar大鼠血糖浓度、肝脏组织病理学改变及FOXA2、FOXA3基因表达的影响

Genetics Applications Pub Date : 2021-12-27 DOI: 10.31383/ga.vol5iss2pp17-23

S. Nikkhah, Rahman Jafari Hafshejan, Farshid Gheibi Hajivar, Khalil Khashei, S. Afzali

{"title":"Effects of Silymarin on Blood Glucose Concentration, Hepatic Histopathological Changes and FOXA2 and FOXA3 Gene Expression in Streptozotocin-Induced Diabetic Male Wistar Rats","authors":"S. Nikkhah, Rahman Jafari Hafshejan, Farshid Gheibi Hajivar, Khalil Khashei, S. Afzali","doi":"10.31383/ga.vol5iss2pp17-23","DOIUrl":"https://doi.org/10.31383/ga.vol5iss2pp17-23","url":null,"abstract":"Since the liver is among the primary organs susceptible to the effects of hyperglycaemia, diabetes mellitus (DM) could be a risk factor for the development and progression of liver damage. In present study, since no side-effects from the herbal medicine have been reported, the effect of silymarin on blood glucose concentration, hepatic histopathological changes and FOXA2 and FOXA3 gene expression, which are key genes in liver regeneration, was investigated. In this fundamental with experimental approach study, 40 male Wistar rats weighing 180-220 g were used. Rats were kept under the standard conditions of temperature of 20-22°C and humidity of 50% and consecutive 12-hour periods of light and darkness. Rats were randomly divided into five different groups (n=8 each), including healthy control rats, diabetic control rats, diabetic rats receiving silymarin (50, 100 and 150 mg/kg). Diabetes was induced by injecting streptozotocin (50 mg/kg B.W., i.p.). For 4 weeks silymarin groups received the drug once every three days through gavage and fasting blood glucose concentration measured once every 10 days. At the end of a month experiment, livers were harvested for hepatic histopathological and FOXA2 and FOXA3 gene expression changes analysis. In the diabetic rats treated with silymarin (50, 100 and 150 mg/kg), by comparison with the diabetic control group (p<0.05), glucose levels decreased significantly. Moreover, FOXA2 and FOXA3 expression in diabetic groups treated with silymarin significantly increased compared to diabetic control group (p<0.05). Hepatic histopathological changes were improved in the treated groups.The present study indicates that silymarin significantly decreased blood glucose concentration and increased the FOXA2 and FOXA3 gene products level. Hence, silymarin is able to improve some of the symptoms associated with diabetes and possesses hepatoprotective effects in streptozotocin-induced diabetic rats.","PeriodicalId":32642,"journal":{"name":"Genetics Applications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44342836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

In vitro assessment of genotoxic and cytotoxic effects of Artemisia annua L. tincture 青蒿酊剂基因毒性和细胞毒性的体外评价

Genetics Applications Pub Date : 2021-12-27 DOI: 10.31383/ga.vol5iss2pp1-9

T. Cetkovic, A. Haverić, Lejla Čaluk Klačar, Maida Hadžić Omanović, S. Haverić

{"title":"In vitro assessment of genotoxic and cytotoxic effects of Artemisia annua L. tincture","authors":"T. Cetkovic, A. Haverić, Lejla Čaluk Klačar, Maida Hadžić Omanović, S. Haverić","doi":"10.31383/ga.vol5iss2pp1-9","DOIUrl":"https://doi.org/10.31383/ga.vol5iss2pp1-9","url":null,"abstract":"The genus Artemisia (fam. Asteraceae) is one of the largest and widely distributed with around 500 species, majority used as aromatic and medicinal plants. Artemisia annua L. is widely used as a dietary spice, herbal tea, as a supplement, and in a non-pharmaceutical form for treatment of malaria and fever. It is orally consumed as capsules, extracts and tinctures and topically applied as an essential oil diluted in lotions and ointments. Artemisinin is the main constituent of Artemisia annua L. extracts. Since the discovery that the artemisinin is efficient in malaria treatment, there is also a growth in consumption of A. annua extracts for antitumour and even recently for antiviral treatments against SARS-CoV-2 infections. This study aimed to investigate genotoxic effect in peripheral blood culture and cytotoxic effects in cancer and normal cell lines, of commercially available A. annua L. tincture in series of dilutions. Both comet and neutral red uptake assays revealed dose-dependent genotoxicity and cytotoxicity of A. annua tincture dilutions. Comet assay revealed significantly increased DNA damage in peripheral blood cells while neutral-red assays showed increase in cytotoxicity (p<0.001) in both normal and cancer cell cultures treated with the lowest extract dilution compared to the highest one applied. Obtained results indicate caution needed in A. annua L. tincture use, especially when poorly diluted.","PeriodicalId":32642,"journal":{"name":"Genetics Applications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44739682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Ion S5 NGS protocol for SARS-CoV-2 genome sequencing 离子S5 NGS方案对SARS-CoV-2基因组测序的评价

Genetics Applications Pub Date : 2021-12-27 DOI: 10.31383/ga.vol5iss2pp24-30

Dino Pećar, Ivana Čeko, Lana Salihefendić, R. Konjhodžić

{"title":"Assessment of Ion S5 NGS protocol for SARS-CoV-2 genome sequencing","authors":"Dino Pećar, Ivana Čeko, Lana Salihefendić, R. Konjhodžić","doi":"10.31383/ga.vol5iss2pp24-30","DOIUrl":"https://doi.org/10.31383/ga.vol5iss2pp24-30","url":null,"abstract":"Monitoring of the lineages SARS-CoV-2 is equally important in a fight against COVID-19 epidemics, as is regular RT - PCR testing. Ion AmpliSeq Library kit plus is a robust and validated protocol for library preparation, but certain optimizations for better sequencing results were required. Clinical SARS-CoV-2 samples were transported in three different viral transport mediums (VTM), on arrival at the testing lab, samples were stored on -20OC. Viral RNA isolation was done on an automatic extractor using a magnetic beads-based protocol. Screening for positive SARS-CoV-2 samples was performed on RT–PCR with IVD certified detection kit. This study aims to present results as follows: impact of first PCR cycle variation on library quantity, comparison of VTMs with a quantified library, maximum storage time of virus and correlation between used cDNA synthesis kit with generated target base coverage. Our results confirmed the adequacy of the three tested VTMs for SARS-CoV-2 whole-genome sequencing. Tested cDNA synthesis kits are valid for NGS library preparation and all kits give good quality cDNA uniformed in viral sequence coverage. Results of this report are useful for applicative scientists who work on SARS-CoV-2 whole-genome sequencing to compare and apply good laboratory practice for optimal preparation of the NGS library.","PeriodicalId":32642,"journal":{"name":"Genetics Applications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45229302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparative analysis of two L-carnitine preparations and their concentration effects on CAT expression in healthy human peripheral blood lymphocyte cultures 两种L-肉碱制剂的比较分析及其浓度对健康人外周血淋巴细胞CAT表达的影响

Genetics Applications Pub Date : 2021-12-27 DOI: 10.31383/ga.vol5iss2pp10-16

M. Kuzmanović, N. Lojo-Kadrić, J. Ramic, A. Haverić, S. Haverić, L. Pojskic

{"title":"Comparative analysis of two L-carnitine preparations and their concentration effects on CAT expression in healthy human peripheral blood lymphocyte cultures","authors":"M. Kuzmanović, N. Lojo-Kadrić, J. Ramic, A. Haverić, S. Haverić, L. Pojskic","doi":"10.31383/ga.vol5iss2pp10-16","DOIUrl":"https://doi.org/10.31383/ga.vol5iss2pp10-16","url":null,"abstract":"CAT gene encodes catalase, a key antioxidant enzyme in the body against oxidative stress. This enzyme plays an important role in the molecular mechanisms of inflammation, apoptosis, mutagenesis and tumorigenesis. Anti-oxidant L-carnitine is used in food supplementation, medical co-treatment and bodyweight regulation. We aimed to investigate molecular basis of L-carnitine commercial preparations supplementation in reducing oxidative stress with customized CAT gene assay in vitro. Human lymphocytes cell culture was established using standard procedure and treated with range of concentrations of L-carnitine in two preparations. We tested two preparations: 500 mg tablets of L-carnitine and liquid L-carnitine with vitamin B6. L-carnitine significantly reduced the expression of CAT gene in cultured lymphocytes at concentrations of 50 μmol/l and 250 μmol/l compared to negative control, (p = 0,001; p = 0,001; respectively). The L-carnitine liquid supplement with vitamin B6 also reduced the transcription of CAT gene at concentrations of 50 μmol/l and 250 μmol/l as compared to the negative control (p = 0,018; p = 0,006; respectively). Selected L-carnitine preparations modulated the transcriptional activity of the antioxidant enzyme gene in human lymphocyte culture, indicating its possible effects in inhibition of pro-inflammatory processes that involve catalase activity.","PeriodicalId":32642,"journal":{"name":"Genetics Applications","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42411398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of Human Pancreatitis-Associated Protein (hPAP) in Komagataella phaffii (Pichia pastoris) 用毕赤酵母（Pichia pastoris）生产人胰腺炎相关蛋白（hPAP）

Genetics Applications Pub Date : 2021-12-27 DOI: 10.31383/ga.vol5iss2pp31-39

Didem Pazarli, Fatıma YÜcel, E. Akçael, Şerife Şeyda Pirinçci Göktürk

{"title":"Production of Human Pancreatitis-Associated Protein (hPAP) in Komagataella phaffii (Pichia pastoris)","authors":"Didem Pazarli, Fatıma YÜcel, E. Akçael, Şerife Şeyda Pirinçci Göktürk","doi":"10.31383/ga.vol5iss2pp31-39","DOIUrl":"https://doi.org/10.31383/ga.vol5iss2pp31-39","url":null,"abstract":"Pancreatitis-associated protein (PAP) is a pancreatic stress protein that is not produced in a healthy pancreas but is highly synthesized in pancreatic acinar cells in response to acute and chronic pancreatitis, hypoxia, toxins, diabetes, lipopolysaccharides hypotransferrinemia and organ transplantation. Changes in the PAP levels in serum are an important biological marker in the early stage of pancreatic diseases. In this study, the recombinant human PAP protein, which has the potential to be used as a diagnostic marker and as research material in proliferation, apoptosis, cell migration, cell invasion, and immunoassay studies, was expressed efficiently under the control of the AOX1 gene promoter in the Komagataella phaffii (Pichia pastoris) (K. phaffii) X33 strain. We describe the conditions required for the efficient production of PAP protein by methanol induction and its use without purification. The produced unpurified protein was tested in sandwich ELISA and showed consistent results with the commercial product. These results are encouraging that the protein produced can be used as a biomarker standard in ELISA tests without the cost and labor of purification.","PeriodicalId":32642,"journal":{"name":"Genetics Applications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45162082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The 2nd Congress of Geneticists in Bosnia and Herzegovina with International Participation 波斯尼亚和黑塞哥维那第二届国际遗传学家大会

Genetics Applications Pub Date : 2021-09-13 DOI: 10.31383/ga.vol0iss0pp1-95

Special Edition BOOK OF ABSTRACTS

引用次数: 0

Association of CYP27B1 Polymorphism and Vitamin D Levels with Multiple Sclerosis Development in Lebanese Population of Bekaa Region: A Preliminary Study 黎巴嫩贝卡地区人群CYP27B1多态性和维生素D水平与多发性硬化症发生的相关性研究

Genetics Applications Pub Date : 2021-06-21 DOI: 10.31383/ga.vol5iss1pp18-25

Sumaia Braidy, Alaa Matar, J. Borjac

{"title":"Association of CYP27B1 Polymorphism and Vitamin D Levels with Multiple Sclerosis Development in Lebanese Population of Bekaa Region: A Preliminary Study","authors":"Sumaia Braidy, Alaa Matar, J. Borjac","doi":"10.31383/ga.vol5iss1pp18-25","DOIUrl":"https://doi.org/10.31383/ga.vol5iss1pp18-25","url":null,"abstract":"Multiple sclerosis (MS) is a neurodegenerative disease of the central nervous system (CNS). Interaction between genetic and environmental factors guides the development of the disease. Among environmental factors, vitamin D deficiency is shown to increase the risk of MS development. Several single nucleotide polymorphisms (SNPs) in cytochrome P450 family 27 subfamily B (CYP27B1) gene that encodes the rate-limiting enzyme involved in vitamin D metabolism, were shown to be correlated with MS. We aimed at investigating the association of CYP27B1 gene polymorphisms and vitamin D level with MS development in a sample of Lebanese MS patients living in the Bekaa region. Enrolled MS patients and controls were age and gender matched. Genotyping was performed by sequencing the amplified CYP27B1 PCR products. Vitamin D levels were measured using a VIDAS® 25 OH Vitamin D total assay based on enzyme linked fluorescent assay (ELFA). Chi-square and Mann-Whitney U tests were used for statistical analysis. A significant association was shown between vitamin D deficiency and MS without any association between CYP27B1 studied SNPs and the disease. We confirmed that vitamin D deficiency was associated with MS with no implication of the studied SNPs of CYP27B1 gene with disease susceptibility among the Lebanese MS patients living in the Bekaa region.","PeriodicalId":32642,"journal":{"name":"Genetics Applications","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44686496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0