Jurnal Akuakultur Indonesia最新文献

{"title":"Cultured sand sea cucumber growth with different water exchange systems","authors":"D. Liubana, Joi Alfreddi Surbakt, Claudya Zyta Tobu","doi":"10.19027/jai.21.2.178-185","DOIUrl":"https://doi.org/10.19027/jai.21.2.178-185","url":null,"abstract":"ABSTRACT \u0000Sand sea cucumber (Holothuria scabra) is a high economic value as a food source. Sea cucumbers contain beneficial bioactive compounds for health. This study determined the sea cucumber growth cultured in an integrated multi-trophic aquaculture (IMTA) with different water exchange systems in Lifuleo waters from October to December, 2020. This study was performed in an experimental method with three different exchange system treatments and three replications, namely once every 2 days (tank A), every day (tank B), and water circulation every day (tank C). Briefly, sand sea cucumbers with 116.72 ± 117.91 g body weight and 11.06 ± 11.83 cm length were maintained for 45 days with different water exchange systems and fed with Eucheuma cottonii. The results showed that the best water exchange systems to increase sand sea cucumber production was tank A with 100% survival rate, 1.84 ± 0.06%/day specific growth rate, and 1714.96 ± 34.13 μm length growth rate. The water quality parameters were also optimal during the sand sea cucumber with the integrated system. \u0000Keywords: Multi-trophic, growth, circulation, integrated system, sand sea cucumber \u0000  \u0000ABSTRAK \u0000Teripang pasir (Holothuria scabra) mempunyai nilai ekonomis tinggi dan diperdagangkan sebagai bahan pangan. Selain itu teripang juga mengandung senyawa bioaktif yang bermanfaat untuk kesehatan. Penelitian ini mengkaji tentang pertumbuhan teripang yang dibudidayakan secara integrated multi-trophic aquaculture (IMTA) dengan sistem pergantian air yang berbeda di perairan Lifuleo pada bulan Oktober sampai Desember 2020. Penelitian ini dilakukan dengan metode eksperimental dengan tiga perlakuan 3 ulangan dengan sistem pergantian air 2 hari sekali (bak A), pergantian air setiap hari (bak B) dan sirkulasi air dilakukan setiap hari (bak C). Secara singkat, teripang dengan bobot badan 116.72 ± 117.91 gr dan panjang 11.06 ± 11.83 cm dipelihara selama 45 hari dengan sistem pergantian air dan diberi makan dengan Eucheuma cottonii. Hasil penelitian menunjukkan bahwa media perlakuan teripang dengan sistem pergantian air yang berbeda terbaik untuk meningkatkan produksi teripang adalah bak A dengan tingkat kelangsungan hidup 100, laju pertumbuhan spesifik harian 1.84 ± 0.06%/hari dan laju pertumbuhan panjang 1714.96 ± 34.13 µm. Parameter kualitas air juga menunjukkan kualitas yang optimal pada budidaya teripang secara terintegrasi. \u0000Kata kunci: Multitropic, pertumbuhan, sirkulasi, terintegrasi, teripang pasir","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80847066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing of black cumin (Nigella sativa) flour to increase the immunity system of tilapia (Oreochromis niloticus) against Aeromonas hydrophila bacteria attack","authors":"Norma Rizki Silviana, Rosidah, Wahyuniar Pamungkas, Roffi Grandiosa","doi":"10.19027/jai.21.2.161-177","DOIUrl":"https://doi.org/10.19027/jai.21.2.161-177","url":null,"abstract":"ABSTRACT \u0000This study aims to determine dose of black cumin flour in fish feed diets which can improve the disease resistance of tilapia against the attack of A. hydrophila. This study was used tilapia juvenile with a size of 7 – 8 cm. This study was conducted from March – May 2021 and the experimental method is completely randomized design (CRD) with 5 treatments and 3 replications. The treatment used were addition of black cumin flour into fish feed with doses 0 (A), 20 (B), 35 (C), 50 (D), and 65 (E) g/Kg feed. The treatments fed by supplemental diets contained black cumin flour was conducted for 14 days, then a challenge test was carried out with the infection of A. hydrophila bacteria by immersion for14 days. A. hydrophila bacteria used to infect fish had a density of 108 CFU/mL. The Parameters used were total leukocyte, hematocrit, total leukocyte differential, clinical symptoms, survival rate and water quality. The result showed that black cumin flour was effective to increase the immunity system of tilapia. The dose of 50 g/Kg feed was effective showed increase the highest percentage increase in white blood cells of 64.56%, compared to treatments B, C, and E which showed an increase in white blood cells of 47.40%, 48.82% and 1.2%, respectively. Treatment A decreased the percentage of white blood cells by 19.52%. The 50 g dose of black cumin showed the highest percentage value of hematocrit and leukocyte differential in tilapia fry, respectively 34%, lymphocytes 84.3%, monocytes 6%, and neutrophils 9.7% with the fastest recovery period (4 days) and after the challenge test resulted in the highest survival rate of 95%. \u0000Keywords: Aeromonas hydrophila, tilapia juvenile, black cumin, immune system \u0000ABSTRAK \u0000Penelitian ini bertujuan untuk menentukan dosis tepung jintan hitam dalam formula pakan ikan yang dapat meningkatkan ketahanan tubuh ikan nila terhadap serangan bakteri A. hydrophila. Penelitian ini menggunakan benih ikan nila dengan ukuran 7-8 cm. Penelitian ini dilaksanakan pada bulan Maret – Mei 2021 dan metode eksperimen yang digunakan adalah model Rancangan Acak Lengkap dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah penambahan tepung jintan hitam pada pakan dengan dosis 0 (A), 20 (B), 35 (C) 50 (D) dan 65 (E) g/Kg pakan. Pemeliharaan ikan uji yang diberikan perlakuan yang mengandung tepung jintan hitam dilakukan selama 14 hari, setelah itu dilanjutkan uji tantang menggunakan bakteri A. hydrophila dengan metode immersi selama 14 hari. Bakteri A. hydrophila yang digunakan untuk menginfeksi ikan memiliki kepadatan 108 CFU/mL. Parameter yang digunakan yaitu leukosit, hematokrit, differensial leukosit, gejala klinis, kelangsungan hidup dan kualitas air. Hasil penelitian memperlihatkan tepung jintan hitam dapat meningkatkan ketahanan tubuh ikan nila. Dosis jintan hitam 50 g per 1 kg pakan paling efektif, terlihat dari persentase peningkatan sel darah putih tertinggi sebesar 64.56%, dibandingkan perlakuan B, C, dan E ","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"125 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76051904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and prevalence of ectoparasites and endoparasites in kerandang fish Channa pleurophtalma and catfish Clarias batrachus captured from Sebangau River","authors":"Infa Minggawati, Frid Agustinus, Tania Serezova Augusta, Candra Putra Pahawang, Toto Francisco","doi":"10.19027/jai.21.2.152-160","DOIUrl":"https://doi.org/10.19027/jai.21.2.152-160","url":null,"abstract":"ABSTRACT \u0000  \u0000This study aimed to determine types, prevalence, and intensity of ectoparasites and endoparasites that infect kerandang fish Channa pleurophtalma and catfish Clarias batrachus. Sampling was carried out in Sebangau River, while identification was carried out at the Fish Quarantine, Quality Control, and Safety Station, Palangka Raya. Examination of ectoparasite infection included bilateral body mucus, caudal fin, and gills. Endoparasite examination was done by observing the fish organs, such as liver, blood, meat, intestines, and stomach. The parasite analysis was determined by calculating the prevalence and intensity. In kerandang fish, the ectoparasites were identified as Trichodina sp., Gyrodactylus sp., Epistylis sp., Dactylogyrus sp., while the endoparasites were Camallanus sp. and Neoechinorhyncus. In catfish, the ectoparasites were identified as Trichodina sp., Dactylogyrus sp., Myxobolus sp., and Costia sp., while the endoparasites were Camallanus sp. Dominant ectoparasite that infected fish was Dactylogyrus sp. on fish gills. For endoparasites, the dominant endoparasites were identified as Neoechinorhyncus in kerandang fish and Camallanus sp. in catfish. The highest prevalence was found in Dactylogyrus sp. at 27%. The prevalence was categorized as a frequent infection. The highest ectoparasite intensity level was obtained from Trichodina sp. at 20.3 ind/fish in kerandang fish and Dactylogyrus sp. at 12.2 ind/fish in catfish. This intensity level is categorized in a medium intensity. \u0000Keywords: Channa pleurophtalma, Clarias batrachus, ectoparasites, endoparasites \u0000  \u0000ABSTRAK \u0000  \u0000Penelitian ini bertujuan untuk mengetahui jenis ektoparasit dan endoparasit yang menginfeksi ikan kerandang Channa pleurophtalma dan ikan lele Clarias batrachus serta prevalensi dan intensitas parasitnya. Pengambilan sampel dilakukan di sungai Sebangau sedangkan identifikasi dilaksanakan di Stasiun Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan Palangka Raya. Pemeriksaaan infeksi ektoparasit meliputi; lendir tubuh bilateral, sirip ekor, dan insang. Pemeriksaan endoparasit dilakukan dengan cara mengamati bagian organ tubuh ikan seperti hati, darah, daging, usus, dan lambung. Untuk mengetahui tingkat serangan parasit pada ikan dianalisis dengan menghitung prevalensi dan intensitas. Pada ikan kerandang parasit yang teridentifikasi ektoparasit adalah Trichodina sp., Gyrodactylus sp., Epistylis sp., Dactylogyrus sp., dan endoparasit adalah Camallanus sp., dan Neoechinorhyncus. Pada ikan lele parasit ektoparasit yang teridentifikasi adalah Trichodina sp., Dactylogyrus sp., Myxobolus sp., dan Costia sp., sedangkan pada endoparasit adalah Camallanus sp. Ektoparasit yang dominan menginfeksi kedua ikan ini adalah Dactylogyrus yang terdapat pada insang. Sedangkan untuk endoparasit, pada ikan kerandang endoparasit yang dominan adalah Neoechinorhyncus dan endoparasit pada ikan lele adalah Camallanus sp. Prevalensi tertinggi pada ikan kerandang dan ik","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"135 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86299760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of fermented rice bran to replace soybean meal in the diet of Clarias sp. fingerling","authors":"A. N. Putra, M. B. Syamsunarno, D. Rohayati, Yuhana Marta Sarah, Ghulam Abdul Matien","doi":"10.19027/jai.21.2.142-151","DOIUrl":"https://doi.org/10.19027/jai.21.2.142-151","url":null,"abstract":"Rice bran is being fermented using Aspergillus niger in order to increase its usage as a raw material for the catfish diet. This research aims to evaluate the potentials of rice bran fermented with A. niger to replace soybean meal in catfish Clarias sp diet. There were two research stages, first the fermentation test of A. niger on rice bran that used a completely randomized factorial design consisting of 2 factors, namely the dose of A. niger (0, 0.5, 1, 1.5, and 2 g/100 g) and incubation time (0. 24 and 48 hours). Secondly, the substitution test of soybean meal with fermented rice bran on five different of substitution levels (0, 15, 20, 35, and 40%) in the catfish diet. The results showed that the fermentation of A. niger of 2 % with an incubation period of 24 hours in rice bran resulted in the smallest crude fiber and fraction content, and increased protein and amino acid including serine, threonine, alanine, methionine, isoleucine, and lysine. The specific growth rate in the substitution treatment of 0-20% was significantly (P<0.05) higher than that of 35-40%. There was also no difference (P>0.05) in the growth performance of catfish in the 0 to 20% substitution treatment. Also, replacing soybean meal with fermented rice bran up to 20% did not have a negative effect on the growth performance of catfish. It was concluded that fermented rice bran could be used as raw material for catfish.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88545210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analisis Ketersediaan Benih Bening Lobster (BBL) Hasil Tangkapan Alam untuk Kegiatan Budidaya Pendederan Lobster","authors":"I. Diatin, Irzal Effendi, Y. Hadiroseyani, T. Budiardi, Virta Rizki Hernanda, Nidwidyanthi Nidwidyanthi, Apriana Vinasyiam","doi":"10.19027/jai.21.2.133-141","DOIUrl":"https://doi.org/10.19027/jai.21.2.133-141","url":null,"abstract":"Indonesia merupakan salah satu negara yang melakukan ekspor lobster ke negara Hongkong, Vietnam, China, dan Singapura, dengan nilai ekspor lobster Indonesia mengalami rata-rata pertumbuhan 3,54% pertahun. Produksi lobster tersebut masih mengandalkan hasil tangkapan, karena budidaya lobster belum banyak dilakukan di Indonesia. Pantai Barat Provinsi Lampung dikenal sebagai daerah tangkapan lobster yang dimanfaatkan oleh nelayan setempat sebagai sumber pendapatan utama. Untuk mengetahui potensi ekonomi dari budidaya lobster, maka dilakukan penelitian dengan tujuan menganalisis ketersediaan benih bening lobster (BBL) dan  rantai pasok lobster sebagai suplai benih untuk kebutuhan budidaya lobster. Metode penelitian ini dilakukan dengan metode survei di Kecamatan Krui Selatan, Kabupaten Pesisir Barat, Lampung. Responden dalam penelitian adalah para nelayan penangkap BBL dan pengepul BBL. Penentuan responden dilakukan dengan metode snowball sampling. Hasil penelitian menunjukkan bahwa jenis lobster utama yang ditangkap adalah jenis lobster pasir dan lobster Mutiara.  Hasil tangkapan nelayan lobster pada bulan gelap yaitu rata-rata 121 340 ekor BBL/bulan, sedangkan pada bulan terang rata-rata 4 680 ekor BBL/bulan, sehinnga dalam setahun benih yang dihasilkan mencapai 1.500.000 ekor BBL. BBL hasil tangkapan nelayan akan dijual pengepul kecil, selanjutnya ke pengepul besar, distributor dan eksportir. BBL terdistribusi ke berbagai wilayah mencakup Lampung, Bengkulu, Jambi, Palembang dan Jakarta. Marjin pemasaran yang diperoleh pengepul kecil dan besar mencapai 15 %.  Produksi benih lobster yang melimpah di perairan Pantai Pesisir Barat Lampung sangat berpotensi untuk pengembangan budidaya pendederan lobster sebagai sumber ekonomi baru.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82858194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormonal induction of gonad maturation in female tinfoil barb fish (Barbonymus schwanenfeldii) using spawnprim hormone","authors":"Amalia Sutriana, Ahmad Baihaqi, Iwan Hasri, Nurliana, Hafizuddin Hafizuddin","doi":"10.19027/jai.21.2.118-124","DOIUrl":"https://doi.org/10.19027/jai.21.2.118-124","url":null,"abstract":"ABSTRACT \u0000  \u0000The aim of this study was to analyze the effect of spawnprim hormone induction with different doses on gonadal maturity of tinfoil barb fish broodstock (Barbonymus schwanenfeldii). A completely randomized design (CRD) was used in this study with 4 treatments and 3 replications. Fish broodstock in group A (control) was not given any treatment, while fish broodstocks in groups B, C, and D were injected with spawnprim hormone at doses of 0.3 ml/kg body weight, 0.6 ml/kg body weight, and 0.9 ml/kg body weight, respectively. The measured parameters were gonad maturity level, broodstock weight gain, egg diameter, and fecundity. Data were analyzed using one way analysis of variance (ANOVA). The result showed that the gonad maturity level (GML) in groups B, C, and D (GML III and IV) was better than group A (GML I). Statistical analysis showed that the induction of spawnprim hormone on tinfoil barb broodstocks significantly affect (P<0.05) broodstock weight gain, egg diameter, and fecundity. The optimal dose of spawnprim hormone for tinfoil barb was 0.6 ml/kg body weight, with the average broodstock weight gain, egg diameter, and fecundity were 0.011 kg, 1.55 mm, and 102.15 eggs, respectively. The induction of spawnprim hormone has a positive effect on gonad maturation of tinfoil barb fish (B. schwanenfeldii), with the optimum dose is 0.6 ml/kg fish \u0000  \u0000Keywords: gonad maturity, tinfoil barb fish, spawnprim \u0000  \u0000ABSTRAK \u0000  \u0000Penelitian ini bertujuan untuk menganalisis pengaruh penggunaan hormon spawnprim dengan dosis yang berbeda terhadap kematangan gonad induk ikan lemeduk Barbonymus schwanenfeldii. Metode yang digunakan pada penelitian ini adalah metode rancangan acak lengkap (RAL) dengan 4 kelompok perlakuan dan 3 kali pengulangan. Ikan pada kelompok A (kontrol) tidak diberikan perlakuan, sedangkan ikan pada kelompok B, C, dan D masing-masing diinjeksi hormon spawnprim dengan dosis 0.3 ml/kg bobot badan; 0.6 ml/kg bobot badan; dan 0,9 ml/kg bobot badan. Parameter yang diamati adalah persentase induk matang gonad akhir, pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Data dianalisis dengan menggunakan analisis varians (ANAVA) pola satu arah. Hasil penelitian menunjukkan tingkat kematangan gonad (TKG) pada kelompok perlakuan B, C, dan D (TKG III dan IV) lebih baik dari kelompok A (TKG I). Hasil uji ANAVA menunjukkan bahwa pemberian hormon spawnprim pada induk ikan lemeduk berpengaruh nyata (P<0.05) terhadap pertambahan bobot induk, pertambahan diameter telur, dan fekunditas. Tingkat kematangan gonad terbaik pada penelitian ini diperoleh pada kelompok perlakuan C yang diinduksi hormon spawnprim dengan dosis 0.6 ml/kg bobot badan induk ikan dengan rata - rata pertambahan bobot induk, pertambahan diameter telur, dan fekunditas masing-masing adalah 0.011 kg, 1.55 mm, dan 102.15 butir telur. Induksi hormon spawnprim menimbulkan pengaruh yang positif terhadap pertambahan bobot induk, diameter telur dan fekunditas ikan lemeduk (B","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77078013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field Experiment: Synbiotic Microcapsule Dietary Supplementation Improved the Health Status of the White Shrimp under the Challenge of Coinfection Diseases through Improvement the Beneficial Microbial Composition.","authors":"Munti Yuhana, Andreas Tambun, W. Widanarni, U. Afiff","doi":"10.19027/jai.21.2.125-132","DOIUrl":"https://doi.org/10.19027/jai.21.2.125-132","url":null,"abstract":"This study aimed to evaluate the effectivity of microencapsulated synbiotic (MS), Bacillus sp. NP5 and mannan oligosaccharide (MOS) dietary in different feeding frequencies in Pacific white shrimp culture field experiment. The MS was administered as a feed supplementation to enhance the immunity for prevention against co-infection with WSSV (White Spot Syndrome Virus) and Vibrio harveyi. The synbiotic was microencapsulated by the spray dryer method. Shrimps were reared in the floating net cages in the pond. Treatments included the administration of MS at different frequencies i.e, daily (A), twice a week (B), once a week (C), and without MS supplementation (consisted of negative and positive controls) with a feeding rate of 6% of shrimp biomass (5 times a day). During the challenge trial, shrimps were removed and further reared in plastic tanks, for 7 days. The shrimps (except negative control treatment) were intramuscularly injected by WSSV filtrate at the infective dosage of 10-4 copies.ml-1. Twenty four hours after WSSV injection the shrimps were immersed in the water-containing cells suspension of V. harveyi at the cell’s population dosage of 106 CFU.ml-1. Immune responses were observed for 7 days after experimental infection. The shrimps that have been treated with daily MS supplementation (A) showed better immune responses i.e., total haemocyte counts, phenoloxidase, respiratory burst, and the lower pathogenic cells abundance in the intestine compared to other treatments groups.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79386331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KINERJA PRODUKSI PENDEDERAN IKAN SIDAT Anguilla bicolor bicolor HASIL GRADING PADA SISTEM RESIRKULASI","authors":"T. Budiardi, I. Effendi, Muhammad Aghistni Rahman, Apriana Vinasyiam","doi":"10.19027/jai.21.2.109-127","DOIUrl":"https://doi.org/10.19027/jai.21.2.109-127","url":null,"abstract":"The growth rate highly varies in nurseries of eel. Variations in size lead to competition in obtaining feed, this causes stunting of smaller fish. This situation leads to high production costs due to poor feed utilization efficiency. Grading needs to be done periodically to improve nursery production performance. Water quality is controlled by a recirculation system that can support production performance through the degradation of toxic compounds. This study aims to analyze the production and nursery performance of graded eel (Anguilla bicolor bicolor) that graded in the same batch in a recirculation system. Completely randomized design (CRD) consisting of three treatments with four replications was used in this research. The treatments included nursery of graded eels for 60 days in three groups of initial weight size, namely 0,35±0,00 g (A); 0,50±0,00 g (B); and 1,04±0,00 g (C). There were 344 eels (A), 239 eels (B), and 116 eels (C) in each replication. The best nursery production performance was obtained in the treatment of 1,04±0,00 g, and the best nursery business performance was obtained in the treatment of 0,50±0,00 g.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87148790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effectiveness of Chitosan to Increase Body Resistance of Sangkuriang Catfish (Clarias gariepinus) Against Aeromonas hydrophila Attack","authors":"Mahatma Kresnha Radityo, Rosidah, Walim Lili, Roffi Grandiosa Herman","doi":"10.19027/jai.21.2.93-108","DOIUrl":"https://doi.org/10.19027/jai.21.2.93-108","url":null,"abstract":"This research aims to determine the optimal dose of chitosan that is appropriate to be given to commercial feed in increasing the immune system of sangkuriang catfish fry. The research was carried out in April – June 2021 at the Aquaculture Laboratory and Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Padjadjaran University. This research used an experimental method of Completely Randomized Design (CRD) with five treatments and three replications. The treatment used was the addition of chitosan to feed with doses of 0 g (A), 2.5 g (B), 5 g (C), 7.5 g (D), and 10 g (E). Maintenance of test fish for 14 days. Experimental fish were infected by Aeromonas hydrophila bacteria with a density of 108 CFU/ml with 10 ml immersion method. Parameters observed included white blood cells, leukocyte differential, clinical symptoms, survival and water quality. Data on white blood cell count, and survival were analyzed by ANOVA, if there was a significant difference, Duncan's test was performed. Leukocyte differential data, clinical symptoms and water quality were analyzed descriptively. The results showed that the addition of chitosan at a dose of 10 g was effective for improving the immune system performance of catfish fry. During 14 days of chitosan administration, the white blood cell count increased by 41.60% from the value of 13.96 × 104 cells/mm3 to 23.90 × 104 cells/mm3. The 10 g chitosan treatment was an effective dose to stimulate differential leukocytes in catfish to improve the immune system, it was also marked by the fastest clinical symptom recovery period and the highest survival rate after the challenge test, which was 96.6%.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79222527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and molecular detection of pathogenicity and antibioticresistance genes in Vibrio parahaemolyticus isolated from pacific white shrimp","authors":"Muhammad Arif Mulya, Fachriyan Hasmi Pasaribu, Usamah Afiff, Munti Yuhana","doi":"10.19027/jai.21.1.81-92","DOIUrl":"https://doi.org/10.19027/jai.21.1.81-92","url":null,"abstract":"ABSTRACT \u0000  \u0000This study aimed to conduct the characterization and molecular detection of the pathogenicity and antibiotic-resistance genes in Vibrio parahaemolyticus, as the causative agent of vibriosis in Pacific white shrimp. The V. parahaemolyticus isolates were collected from the shrimp’s hepatopancreas, before biochemical test and polymerase chain reaction (PCR) sequencing of the 16S rRNA gene confirmation. The hemolysis test and PCR were applied to detect the presence of virulence genes, namely toxR, thermostable direct haemolysin (tdh), and tdh-related haemolysin (trh). The Kirby-Bauer method was used for characterizing the resistance patterns against ampicillin (AMP), tetracycline (TET), cyprofloxacin (CIP), enrofloxacin (ENR), and chloramphenicol (CHL). The biochemical tests and PCR-16SrRNA gene sequencing confirmed that 12 isolates belonged to V. parahaemolyticus that were further verified by amplification of the toxR gene in 382 bp (100% of the isolates). The alpha hemolysis activity was also confirmed by the amplicon of 199 bp in all isolates. All V. parahaemolyticus isolates showed their resistance to AMP and 42% of the isolates were TET-resistant. However, no resistance was shown to CIP, ENR, and CHL. The PCR-based analysis resulted a detectable resistance gene of ampC (42% of the isolates) and tetB (83% of the isolates). \u0000Keywords: antibiotics, shrimp, resistance, virulency, Vibrio parahaemolyticus \u0000  \u0000ABSTRAK \u0000  \u0000Penelitian ini bertujuan untuk melakukan karakterisasi dan deteksi molekular dari gen patogenisitas dan resistansi antibiotik pada Vibrio parahaemolyticus, agen penyebab vibriosis pada udang vaname. Isolat V. parahaemolyticus dikoleksi dari hepatopankreas, diuji secara biokimiawi dan selanjutnya dikonfirmasi dengan polymerase chain reaction (PCR)-sekuensing dari gen 16S rRNA. Tes hemolisis dan metode PCR diterapkan untuk mendeteksi keberadaan gen virulensi toxR, thermostable direct haemolysin (tdh) and tdh-related haemolysin (trh). Metode Kirby Bauer digunakan untuk karakterisasi pola resistansi terhadap ampisilin (AMP), tetrasiklin (TET), kloramfenikol (CHL), siprofloksasin (CIP) dan enrofloksasin (ENR). Uji biokimia dan sekuensing gen PCR-16SrRNA memastikan bahwa 12 isolat adalah V. parahaemolyticus yang selanjutnya diverifikasi dengan amplifikasi gen toxR berukuran 382 bp (100% isolat). Aktivitas alfa hemolisis juga dikonfirmasi dengan amplikon PCR (199 bp) di semua isolat. Seluruh isolat V. parahaemolyticus menunjukkan resistansinya terhadap AMP, 42% resistan TET, tidak ada resistansi yang ditunjukkan pada CIP, ENR dan CHL. Analisis berbasis PCR menghasilkan gen resistan yang terdeteksi dari gen ampC (42% isolat) dan gen tetB (83% isolat). \u0000Kata kunci: Antibiotik, udang, resistansi, virulensi, Vibrio parahaemolyticus","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85240659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}