The NucleusPub Date : 2024-02-06DOI: 10.1007/s13237-023-00464-0
Dilipraj Sathyamurthy, Jayakanthan Mannu, Senthil Natesan, Bharathi Nathan, Saranya Nallusamy, M. B. Narayanan, Kavithamani Duraisamy
{"title":"Comparative chloroplast genome analysis of six millet species along with related Poaceae family members","authors":"Dilipraj Sathyamurthy, Jayakanthan Mannu, Senthil Natesan, Bharathi Nathan, Saranya Nallusamy, M. B. Narayanan, Kavithamani Duraisamy","doi":"10.1007/s13237-023-00464-0","DOIUrl":"https://doi.org/10.1007/s13237-023-00464-0","url":null,"abstract":"","PeriodicalId":31888,"journal":{"name":"The Nucleus","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139860063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The NucleusPub Date : 2024-02-06DOI: 10.1007/s13237-023-00464-0
Dilipraj Sathyamurthy, Jayakanthan Mannu, Senthil Natesan, Bharathi Nathan, Saranya Nallusamy, M. B. Narayanan, Kavithamani Duraisamy
{"title":"Comparative chloroplast genome analysis of six millet species along with related Poaceae family members","authors":"Dilipraj Sathyamurthy, Jayakanthan Mannu, Senthil Natesan, Bharathi Nathan, Saranya Nallusamy, M. B. Narayanan, Kavithamani Duraisamy","doi":"10.1007/s13237-023-00464-0","DOIUrl":"https://doi.org/10.1007/s13237-023-00464-0","url":null,"abstract":"","PeriodicalId":31888,"journal":{"name":"The Nucleus","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139800044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The NucleusPub Date : 2023-12-15DOI: 10.1007/s13237-023-00457-z
Sandeep Jaiswal, Lal Bahadur Singh, Kuldeep Kumar, J. Vijayan, Nivedita Shettigar, Sudhir Kumar Jha, K. R. Soren
{"title":"Anti-CRISPR proteins: a weapon of phage-bacterial arm race for genome editing","authors":"Sandeep Jaiswal, Lal Bahadur Singh, Kuldeep Kumar, J. Vijayan, Nivedita Shettigar, Sudhir Kumar Jha, K. R. Soren","doi":"10.1007/s13237-023-00457-z","DOIUrl":"https://doi.org/10.1007/s13237-023-00457-z","url":null,"abstract":"","PeriodicalId":31888,"journal":{"name":"The Nucleus","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139000535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The NucleusPub Date : 2023-12-05DOI: 10.1007/s13237-023-00452-4
Sushma Devi, J. K. Raina, Pawan Kumar, Amrit Sudershan, Ravi Sharma, Rakesh K. Panjalyia, Sanjeev K. Digra, Parvinder Kumar
{"title":"Genetic association study of rs1801133 of MTHFR, rs4646903 of CYP1A1, null mutation of GSTM1&GSTT1 with leukemia in the Jammu region of north Indian population","authors":"Sushma Devi, J. K. Raina, Pawan Kumar, Amrit Sudershan, Ravi Sharma, Rakesh K. Panjalyia, Sanjeev K. Digra, Parvinder Kumar","doi":"10.1007/s13237-023-00452-4","DOIUrl":"https://doi.org/10.1007/s13237-023-00452-4","url":null,"abstract":"","PeriodicalId":31888,"journal":{"name":"The Nucleus","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138601155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The NucleusPub Date : 2023-12-01DOI: 10.1007/s13237-023-00454-2
Rohith Grandhi, Ingrid Berenice Sanchez Carrillo, Mariia Synytsia, Hugo Germain
{"title":"Architecture and composition of plant nucleopore complexes, comparisons with putative homologs across kingdoms","authors":"Rohith Grandhi, Ingrid Berenice Sanchez Carrillo, Mariia Synytsia, Hugo Germain","doi":"10.1007/s13237-023-00454-2","DOIUrl":"https://doi.org/10.1007/s13237-023-00454-2","url":null,"abstract":"","PeriodicalId":31888,"journal":{"name":"The Nucleus","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138611886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The NucleusPub Date : 2023-11-13DOI: 10.1007/s13237-023-00451-5
Douglas E. Soltis, Jennifer A. Tate, Pamela S. Soltis, V. Vaughan Symonds
{"title":"Parental genomic compatibility model: only certain diploid genotype combinations form allopolyploids","authors":"Douglas E. Soltis, Jennifer A. Tate, Pamela S. Soltis, V. Vaughan Symonds","doi":"10.1007/s13237-023-00451-5","DOIUrl":"https://doi.org/10.1007/s13237-023-00451-5","url":null,"abstract":"","PeriodicalId":31888,"journal":{"name":"The Nucleus","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136283591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The NucleusPub Date : 2023-11-07DOI: 10.1007/s13237-023-00449-z
Xianwen Ji, Saulo Alves Aflitos, Sander Peters, M. Eric Schranz, Jirska Philipse-Berendsen, Aat Vogelaar, Cilia Lelivelt, Hans de Jong
{"title":"FISH painting for chromosome identification of aneuploid cauliflower (Brassica oleracea L. var. botrytis)","authors":"Xianwen Ji, Saulo Alves Aflitos, Sander Peters, M. Eric Schranz, Jirska Philipse-Berendsen, Aat Vogelaar, Cilia Lelivelt, Hans de Jong","doi":"10.1007/s13237-023-00449-z","DOIUrl":"https://doi.org/10.1007/s13237-023-00449-z","url":null,"abstract":"Abstract A common problem in the cultivation and breeding of cauliflower ( Brassica oleracea L. var. botrytis ) is the occurrence of aneuploids in offspring families. To reveal the chromosomal cause of such numerical variants, it was necessary to develop karyotype tools with which chromosomes can be easily identified. Since mitotic chromosomes in this crop are morphologically similar and lack differentiating banding patterns, we tested two Fluorescent in situ Hybridization (FISH) procedures for chromosome identification: (1) FISH painting with diagnostic repetitive DNA patterns and (2) cross-species chromosome painting. The first method consists of a five-colour FISH with 5s rDNA, 45S rDNA, and two Brassica rapa centromere-specific repeats, and a B. rapa BAC (KBrH092N02) containing a dispersed repeat of an unknown class. The second method is an advanced FISH technology based on hybridising DNA probes of a related species under adapted stringency conditions to identify their homoeologous loci. To this end, we applied four pools of BACs from Arabidopsis thaliana in a multicolour FISH for a banding pattern on the chromosomes of cauliflower ( Brassica oleracea L. var. botrytis ). Due to the genome triplication and various chromosome rearrangements of Brassica oleracea compared to Arabidopsis, we used MUMmer whole-genome alignment plot information to select Arabidopsis BAC pools with which all cauliflower chromosomes could be identified. In a sample of 21 plants with aberrant phenotypes, we demonstrated primary trisomy for chromosomes 1–6 and 8, and telo-trisomy for chromosomes 7 and 9. Finally, we discuss the advantages and drawbacks of the two painting methods and eventual alternatives for demonstrating numerical aberrations in the cauliflower populations. Graphical Abstract","PeriodicalId":31888,"journal":{"name":"The Nucleus","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135475479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The NucleusPub Date : 2023-11-01DOI: 10.1007/s13237-023-00445-3
Lakshminarasimhan Krishnaswamy, Changzeng Zhao, Patrice S. Albert, Zhi Gao, Ellen Mazalale, Alessandra Torno York, Louis Nastasi, Jacob A. Kelly, Hua Yang, James A. Birchler
{"title":"Transgenic direct terminal repeats of Dissociation (Ds) produce chromosomal breakage in maize","authors":"Lakshminarasimhan Krishnaswamy, Changzeng Zhao, Patrice S. Albert, Zhi Gao, Ellen Mazalale, Alessandra Torno York, Louis Nastasi, Jacob A. Kelly, Hua Yang, James A. Birchler","doi":"10.1007/s13237-023-00445-3","DOIUrl":"https://doi.org/10.1007/s13237-023-00445-3","url":null,"abstract":"Abstract Barbara McClintock recognized transposable elements originally by the movement of a site of chromosomal breakage, a genetic element called Dissociation ( Ds ) that was induced to break or transpose by another element she called Activator . The chromosome breaking version, when analyzed on the molecular level was one transposon inside another. It is now known that transposition involving transposon termini in non-standard orientation with reference to each other results in chromosomal breakage. Here we used engineered transposon ends together with a phenotypic marker to cause targeted chromosomal breaks. The results indicate that engineered direct orientation of the naturally inverted repeats of Dissociation can cause chromosomal breakage at the transgenic sites of insertion.","PeriodicalId":31888,"journal":{"name":"The Nucleus","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135320506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Karyological diversity among six medicinally important species of Acanthaceae with three new chromosome counts","authors":"Nowshin Anjum, Chandan Kumar Dash, Syeda Sharmeen Sultana","doi":"10.1007/s13237-023-00443-5","DOIUrl":"https://doi.org/10.1007/s13237-023-00443-5","url":null,"abstract":"","PeriodicalId":31888,"journal":{"name":"The Nucleus","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136262177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}