Mayadah A. Haj Ali, Ahmed A. Kheder, Aladdin Hamwieh
{"title":"Analysis of sesame phyllody disease using transmission electron microscopy and intersimple sequence repeat-polymerase chain reaction","authors":"Mayadah A. Haj Ali, Ahmed A. Kheder, Aladdin Hamwieh","doi":"10.23960/jhptt.12584-95","DOIUrl":"https://doi.org/10.23960/jhptt.12584-95","url":null,"abstract":"Sesame phyllody disease caused by phytoplasma has been identified in Egypt as a devastating phytopathogenic mollicute, However, the genetic diversity of phytoplasma infecting sesame plants in Egypt, as revealed by Intersimple Sequence Repeats-Polymerase Chain Reaction (ISSRs-PCR), has not been fully explored. During 2021–2022, sesame plants exhibiting virescence, phyllody, proliferation, and witches’ broom symptoms were observed in various fields across the Faiyoum, Luxor, and Beheira governorates in Egypt. This study aims to identify phytoplasma using transmission electron microscopy (TEM) and ISSRs-PCR. The methodology includes sampling, pathogenicity testing, TEM analysis of ultrathin sections, nested polymerase chain reaction (nested-PCR) targeting the 16S rRNA gene, and clustering analysis using 15 ISSRs primers. Phytoplasma was successfully transmitted to healthy plants through grafting and dodder transmission, with success rates of 80% and 90%, respectively. TEM analysis revealed polymorphic phytoplasma bodies and extensive phloem necrosis. Nested-PCR produced 1250 bp amplicons for all isolates. Phylogenetic analysis showed that the Beheira isolate (“OP185273.1”) is closely related to the peanut WB group. DNA polymorphic fragments ranged from 1 to 10 per profile, with fragment sizes between 150 and 1500 bp. Cluster analysis revealed that only two isolates (Faiyoum and Beheira) clustered together. This study demonstrates the effectiveness of ISSR-PCR in detecting and analyzing phytoplasma infecting sesame plants in Egypt.","PeriodicalId":31803,"journal":{"name":"Jurnal Hama dan Penyakit Tumbuhan Tropika","volume":"25 1","pages":"84-95"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://jhpttropika.fp.unila.ac.id/index.php/jhpttropika/article/download/817/645","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147906458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Supriati, Siti Zubaidah, A. A. Djaya, Oesin Oemar, M. M. Ramadhan
{"title":"Application of Trichoderma Isolate consortium in organic fertilizer for controlling shallot twisted disease","authors":"L. Supriati, Siti Zubaidah, A. A. Djaya, Oesin Oemar, M. M. Ramadhan","doi":"10.23960/jhptt.224237-246","DOIUrl":"https://doi.org/10.23960/jhptt.224237-246","url":null,"abstract":"The aim of the research was to determine the effect of applying a consortium of Trichoderma spp. isolates to a type of organic fertilizer that is effective in controlling twisted disease and on the growth and yield of shallot plants. The study utilized a completely randomized design (CRD) consisting of eight treatments with four replications. The treatments examined werea= as follows: P0 = control, P1 = 5 tons per ha of chicken manure fertilizer, P2 = 5 tons per ha of chicken manure fertilizer and consortium of two Trichoderma spp. isolates, P3 = 5 tons per ha of chicken manure fertilizer and consortium of three Trichoderma spp. isolates, P4 = 5 tons per ha of chicken manure fertilizer, 20 tons per ha trichocompost, and a consortium of two Trichoderma spp. isolates, P5 = 5 tons per ha of chicken manure fertilizer, 20 tons per ha trichocompost, and consortium of three Trichoderma spp. isolates, P6 = 20 tons per ha of trichocompost and a consortium of two Trichoderma spp. isolates, and P7 = 20 tons per ha of trichocompost and a consortium of three Trichoderma spp. isolates. The results showed that the twisted disease incidence in treatment P4 was the lowest (14.52%) at 8 weeks after planting. The plant height was of 39.10 cm, with 29.8 leaves per clump at 7 WAP, and the dried bulb weight was 112.4 g per clump. In conclusion, the application of chicken manure fertilizer at a dose of 5 ton per ha, combined with 20 tons per ha of trichocompost and a consortium of two Trichoderma spp. isolates, could control twisted disease in shallot.","PeriodicalId":31803,"journal":{"name":"Jurnal Hama dan Penyakit Tumbuhan Tropika","volume":"34 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141928411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sigit Prastowo, Widi Kusuma Wardhani, A. P. Pradana, Ali Wafa, H. Addy, Zul Fauzi Nugroho Hadi
{"title":"Metarhizium anisopliae Metsch. (Moniliales: Moniliaceae) and Aglaia odorata Lour. (Sapindales: Maliaceae) leaf extract: a potent biopesticide cocktail for cabbage leaf caterpillar management","authors":"Sigit Prastowo, Widi Kusuma Wardhani, A. P. Pradana, Ali Wafa, H. Addy, Zul Fauzi Nugroho Hadi","doi":"10.23960/jhptt.224216-226","DOIUrl":"https://doi.org/10.23960/jhptt.224216-226","url":null,"abstract":"Plutella xylostella Linnaeus (Lepidoptera: Plutellidaae) is an important pest on Brassicaceae plants, especially cabbage. The combination of entomopathogenic fungi and botanical pesticides has been widely developed to increase effectiveness effectiveness against P. xylostella . This research aimed to determine the potential of Metarhizium anisopliae and Aglaia odorata Leaf Extract (Ao-LE) as a combined control strategy for P. xylostella. The parameters observed in this research were colony growth, viability of conidia of M. anisopliae, antifeedant activity, mortality, and lethal time 50 (LT50) of P. xylostella . The findings revealed that Ao-LE addition maintained colony growth comparable to that of the control treatment, while significantly enhancing the conidial viability of M. anisopliae. Notably, 0.80% and 1.00% Ao-LE in the M. anisopliae suspension resulted in maximum antifeeding activity. Moreover, incorporating 0.20-1.00% Ao-LE demonstrably improved P. xylostella mortality compared with M. anisopliae alone. The combination of M. anisopliae 107 conidia/mL and 1.00% Ao-LE exhibited the lowest LT50 value of 2.16 days, highlighting the synergistic effect of the combined treatment. In conclusion, this study provides compelling evidence for the compatibility and synergistic potential of M. anisopliae and Ao-LE, presenting a promising strategy for the sustainable management of P. xylostella infestations.","PeriodicalId":31803,"journal":{"name":"Jurnal Hama dan Penyakit Tumbuhan Tropika","volume":"34 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141804402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Loekas Soesanto, Ika Vidi Nuraini, Murti Wisnu Ragil Sastyawan, Endang Mugiastuti, N. W. A. Leana, Ruth Feti Rahayuniati
{"title":"Application of Bio P60 and Bio T10 alone or in combination to control Fusarium wilt of Hydroponic Melon","authors":"Loekas Soesanto, Ika Vidi Nuraini, Murti Wisnu Ragil Sastyawan, Endang Mugiastuti, N. W. A. Leana, Ruth Feti Rahayuniati","doi":"10.23960/jhptt.224203-215","DOIUrl":"https://doi.org/10.23960/jhptt.224203-215","url":null,"abstract":"The research aimed to determine the effect of single and combined applications of Bio P60 and Bio T10 in suppressing stem base rot and its effect on the growth and production of hydroponic melon. This research was conducted at Flos Hydroponic Organic at Bansari Village, Bansari District, Temanggung Regency from February to June 2023. Randomized Block Design was used with 6 replicates. The treatments were control (propamocarb hydrochloride), Bio P60, Bio T10, and a combination of Bio P60 and Bio T10 (1:1, v/v). Variables observed were incubation period, disease intensity, infection rate, area under disease progress curve (AUDPC), control effectiveness, plant length, number of leaves, fresh weight, leaf color, first flowering date, first fruit formation, number of fruits per plant, fruit weight per plant, and phenolic compounds qualitatively. The results showed that the combined treatment of Bio P60 and Bio T10 had the best effect indicated by delaying the incubation period, reducing disease intensity, reducing infection rates, reducing AUDPC values, increasing the value of control effectiveness, increasing plant length, number of leaves, plant fresh weight, leaf color, time of first flower appearance, time of fruiting, and fruit weight respectively of 31.25, 41.19, 13.33, 65.31, 55.61, 17.25, 5.57, 36.44, 11.47, 8.55, 9.63, and 22.92 % compared to control. The application of Bio P60, Bio T10, and the combination could increase the phenolic compounds (tannins, saponins, and glycosides) qualitatively in melon leaves.","PeriodicalId":31803,"journal":{"name":"Jurnal Hama dan Penyakit Tumbuhan Tropika","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141813793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cheppy Wati, A. A. Nawangsih, A. Wahyudi, Suryo Wiyono, A. Munif
{"title":"The effectiveness of Liliaceae phyllospheric Actinomycetes as biocontrol agent of purple blotch disease (Alternaria porri Ell. Cif) on shallot","authors":"Cheppy Wati, A. A. Nawangsih, A. Wahyudi, Suryo Wiyono, A. Munif","doi":"10.23960/jhptt.224194-202","DOIUrl":"https://doi.org/10.23960/jhptt.224194-202","url":null,"abstract":"Purple blotch, caused by Alternaria porri, affects leaves and tubers, leading to reduced yields. Traditionally, synthetic chemical fungicides were heavily relied upon for control. As an alternative, biocontrol agents like actinomycetes have gained attention. Some actinomycetes can suppress plant pathogens by producing antifungal compounds. This research aimed to investigate the efficacy of phyllosphere actinomycetes from Liliaceae plants as biocontrol agents against purple blotch disease on shallots in the greenhouse. Conducted at the IPB University Plant Bacteriology and Mycology Laboratory and the greenhouse at Bogor Agricultural Development Polytechnic, West Java, the research involved several stages, including pathogen identification, preparation of actinomyces inoculum and A. porri, and application of actinomycetes biocontrol agents to shallots in the greenhouse. A. porri isolates were obtained from shallot production centers in the Brebes area, Central Java. The use of the actinomycetes from the phyllosphere effectively suppressed purple blotch disease, with the lowest area under the disease development curve (AUDPC) recorded at 635.9% for the CFS28 isolate. Lower AUDPC values indicated slower disease progression. Actinomycetes biocontrol agents showed promising efficacy, with the CFS28 isolate achieving the highest efficacy percentage of 78.37%. Additionally, plant growth was significantly enhanced by actinomycete application, with tuber sizes ranging from 1.44 to 2.06 g, fresh weights from 17.63 to 24.72 g, and dry weights of shallot bulbs from 5.43 to 17.96 g. The incubation period for A. porri could be extended by actinomycetes, ranging from 5.43 to 8.5 days for purple blotch symptoms to manifest on shallots. The use of Actinomyces phyllosphere biocontrol agents holds promise for disease control on other plants’ leaves, contributing to environmentally friendly and sustainable agricultural practices.","PeriodicalId":31803,"journal":{"name":"Jurnal Hama dan Penyakit Tumbuhan Tropika","volume":"62 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141688659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. W. Trisnawati, I. Nurkomar, Andri Antono, Era Puspitasari
{"title":"Diversity and community structure of predators in surjan (polyculture) and lembaran (monoculture) paddy fields","authors":"D. W. Trisnawati, I. Nurkomar, Andri Antono, Era Puspitasari","doi":"10.23960/jhptt.224166-176","DOIUrl":"https://doi.org/10.23960/jhptt.224166-176","url":null,"abstract":"Predator diversity decreases because of low ecosystem quality in modern agriculture that applies monoculture systems. However, polyculture systems in paddy fields can improve biodiversity, including pests, natural enemies, and microorganisms. Surjan is a local polyculture farming practice that originated in Yogyakarta. This farming practice consists of raised beds for cultivating palawija or horticultural crops and sunken beds for rice cultivation. Surjan farming may have an impact on predatory diversity and abundance, although this has not been studied. To address this issue, research was carried out to investigate the diversity, abundance, and structure of the community of predators in the paddy fields of surjan (polyculture) and lembaran (monoculture). The field experiment was conducted in three pairs, surjan and lembaran, in Panjatan District, Kulon Progo Regency, Yogyakarta Special Region, Indonesia. In each field, five random plots arranged in a cross pattern were used to collect a sample of predators. Several traps, including sweeping, pitfall traps, yellow adhesive traps, and yellow pan traps, were used to determine the abundance and diversity of predators. Results showed that species diversity, abundance, species composition, and biodiversity index in surjan farming were significantly improved compared to lembaran farming. Ceratopogonidae and Formicidae were the most abundant families in both surjan and lembaran farming, although they are more abundant in surjan. It can be concluded from the study that surjan farming could improve ecosystem quality by implementing predators for pest management.","PeriodicalId":31803,"journal":{"name":"Jurnal Hama dan Penyakit Tumbuhan Tropika","volume":"68 32","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141346293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Suryo Wiyono, Sri Hendrastuti Hidayat, Sobir, A. S. Suryaningsih
{"title":"The use of combination plant growth promoting rhizobacteria to control chili leaf curl disease in the field","authors":"Suryo Wiyono, Sri Hendrastuti Hidayat, Sobir, A. S. Suryaningsih","doi":"10.23960/jhptt.224177-184","DOIUrl":"https://doi.org/10.23960/jhptt.224177-184","url":null,"abstract":"Plant growth-promoting rhizobacteria (PGPR) is a promising technology for controlling viral diseases, including pepper yellow leaf curl disease (PYLCD) of chili pepper caused by Begomovirus infection. The objectives of this research were to investigate the effectiveness of PGPR containing Pseudomonas fluorescens PF1 and Bacillus polymyxa BG25, as well as their combination with other protective agents, to control PYLCD under field conditions in an endemic region. The treatments consisted of a single application of PGPR (a mixture of P. fluorescens PF1 and B. polymyxa BG25), guano tea, endophytic fungus H5, and neem oil; combination of PGPR with guano tea, endophytic fungus H5, and neem oil; conventional pesticide that relies on synthetic chemical insecticide sprayed weekly; and untreated plots. The experiment was arranged in a randomized block design with four replications. Treatment with PGPR alone was able to delay disease onset by 2.25 weeks, but it caused only a slight reduction in disease incidence. The combination of PGPR + guano tea and PGPR + endophyte H5 provided the best results in controlling PYLCD. The combination of PGPR + guano tea and PGPR + endophyte H5 delayed disease onset by 2.75 weeks and 3.25 weeks, respectively, and reduced disease incidence with effectiveness rates of 52.72% and 52.08%, respectively. These two treatment combinations gave the best performance for plant growth and yield.","PeriodicalId":31803,"journal":{"name":"Jurnal Hama dan Penyakit Tumbuhan Tropika","volume":"62 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141349349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research trend of bacterial leaf blight on rice in the millennium era: a bibliometric and scientometric approach for capture future insight","authors":"Syahri, R. U. Somantri","doi":"10.23960/jhptt.224139-153","DOIUrl":"https://doi.org/10.23960/jhptt.224139-153","url":null,"abstract":"In the 21st century, population growth affects food demand, especially rice. Xanthomonas oryzae pv. oryzae causes bacterial leaf blight (BLB) disease, the worldwide rice production problem. As pathogen pathotypes vary, research into disease control advances rapidly. Thus, these trends must be mapped to inform future studies. This study aims to examine previous research using bibliometric and scientometric methods to inform future research. Scopus publication data was analyzed utilizing VOSviewer and CiteSpace. Research progress, popular research, productive countries, productive researchers, essential keywords, and global collaboration were explored. Analysis of recent research data predicts future trends. The findings indicate that 21st-century BLB research is advancing at an astounding rate and significantly surpasses that of previous periods, with 596 articles (91.69%) published during this era. Agricultural and Biological Sciences is the leading focus due to its substantial emphasis on genetics-related issues. A statistical analysis of the most prolific countries over the past two decades reveals that the United States, China, and India produce the highest number of articles. China and India are the two largest rice producers, respectively. Nevertheless, the most productive authors reveal that Szurek B (a French scientist) ranks first with a staggering twenty articles. Three main research clusters were found that consisted of “antibacterial agents vs. bacterial disease,” “plant resistance and immunity mechanisms,” and “pathogen virulence and pathogenicity genes.” The study about resistance genes against disease became the most cited article. We concluded that there are several future insights, including “further investigation into the alternative antibacterial agent and their formulation,” “spatial distribution, severity, and prevalence of BLB in multiple conditions,” “the microbial community on plant leaves,” and “additional research to comprehend the mechanism of the microbial community in the rice rhizosphere.” In addition, research on the stability of plant resistance genes to anticipate changes in pathogen pathotypes will be an alternative topic.","PeriodicalId":31803,"journal":{"name":"Jurnal Hama dan Penyakit Tumbuhan Tropika","volume":"5 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141117608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effect of tobacco leaf extract dosage on the development of virus disease in curly chili","authors":"H. Haryuni, S. Priyadi","doi":"10.23960/jhptt.124120-127","DOIUrl":"https://doi.org/10.23960/jhptt.124120-127","url":null,"abstract":"Curly chili is a horticultural product with high nutritional value, greatly demanded as a distinctive complement or flavoring for dishes due to its unique taste and aroma. Despite the increasing demand for chilies, production has not followed suit, mainly due to disruption caused by viruses transmitted by Bemisia tabaci and Thrips sp. This study aimed to investigate the effect of tobacco extract on the growth and development of viral diseases in curly chili. Conducted at Kwangsang Village, Jumapolo District, Karanganyar Regency, from September to December 2021, the research was designed using a completely randomized block design, with treatment doses of tobacco extract at 0 mL/L, 1 mL/L, 2 mL/L, 3 mL/L, and 4 mL/L, labeled J0, J1, J2, J3, and J4, respectively. The results indicated that the variabels, i.e. plant height, number of leaves, number of fruits, and fruit weight per plant, were significantly affected by the tobacco extract. Similarly, the disease incidence and severity of viral infection were significantly influenced by the tobacco extract application. The yield decrease in curly chili was attributed to virus infection by Bemisia tabaci and Thrips sp., which caused chlorosis and mosaic symptoms on the leaves. Although the affected plants did not die, they experienced a yield reduction. The application of tobacco extract significantly reduced the percentage of incidence and severityof virus infection on curly chili. The highest disease incidence was 35.42% (J0) and disease severity was 70.92% (J0), while the lowest disease incidence was 14.58% (J4) and disease severity was 20.58% (J4).","PeriodicalId":31803,"journal":{"name":"Jurnal Hama dan Penyakit Tumbuhan Tropika","volume":"70 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Prasetyo, Nur Baitullah Juniar, R. Evizal, T. Maryono, Cipta Ginting
{"title":"Molecular identification of Trichoderma sp. Margodadi isolate and its potential against Phytophthora capsici causing foot rot of black pepper","authors":"J. Prasetyo, Nur Baitullah Juniar, R. Evizal, T. Maryono, Cipta Ginting","doi":"10.23960/jhptt.124128-138","DOIUrl":"https://doi.org/10.23960/jhptt.124128-138","url":null,"abstract":"Trichoderma has the potential to suppress fungal pathogens and thus control plant diseases, including Phytophthora foot rot, which is the most devastating disease of black pepper in Lampung. Identification of a microorganism can not only rely on its morphological characteristics, but it is also necessary to identify it molecularly at the species level. This research was aimed at identifying the fungus Trichoderma sp. Margodadi isolates at the species level and to know the potential of Trichoderma sp. Margodadi isolates and their secondary metabolites to control P. capsici. This research was conducted from March to November 2021 at the Laboratory of Plant Disease, Department of Plant Protection, and the Laboratory of Agricultural Biotechnology, Faculty of Agriculture, University of Lampung. Identification of Trichoderma was done by morphological characteristics and molecular methods. The ability of Trichoderma to suppress P. capsici was tested by dual culture. The effect of secondary metabolites on the growth of P. capsici was determined in vitro at concentrations of 0% (control), 10%, 20%, 30%, and 40%. The experimental design used was a completely randomized design consisting of five treatments repeated five times. The data obtained from the test were analyzed using ANOVA, followed by the LSD test at 5%. The results of this study showed that Trichoderma sp. Margodadi isolate had a close relationship with Trichoderma asperellum and had the ability as an antagonist to inhibit the growth of P. capsici up to 47.23%, and the secondary metabolites produced could inhibit the growth of P. capsici up to 72.53% with the best concentration of 40%.","PeriodicalId":31803,"journal":{"name":"Jurnal Hama dan Penyakit Tumbuhan Tropika","volume":"32 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}