A. Hayati, Nita Yuliarini, A. Sugianto, Hana Widyana, Inesavira Windaputri, Nuris Aulia, Putri Ayu Ika
{"title":"Metallothionein analysis and cell damage levels on the liver and gill Of Barbonymus gonionotus In Brantas River, Indonesia","authors":"A. Hayati, Nita Yuliarini, A. Sugianto, Hana Widyana, Inesavira Windaputri, Nuris Aulia, Putri Ayu Ika","doi":"10.23869/BPHJBR.23.1.20174","DOIUrl":"https://doi.org/10.23869/BPHJBR.23.1.20174","url":null,"abstract":"This study aimed to analyze the levels of metallothionein and damage to hepatocytes and gills of Barbonymus gonionotus in the Brantas River (upstream and downstream), and the correlation levels of metallothionein in the gills and liver of Barbonymus gonionotus with different sampling time variation (March, June, and September 2016). Fishes were caught using trawl in two stations (Karangkates Reservoir and the Kali Jagir river). Fish gills and livers were taken for histopathological analysis and levels of metallothionein were measured by ELISA method. Gills histopathological analysis showed that the highest damage in Karangkates reservoir dan Kali Jagir river is hyperplasia. Liver histopathological analysis in Karangkates reservoir showed normal hepatocytes meanwhile necrosis is highest damage found in Kali Jagir river. Metallothionein levels of fish in Karangkates reservoir for March, June, and September were 0.18 ng/mL; 0,18 ng/mL; and 0.2 ng/mL (gills fish); 0,18 ng/mL; 0,34 ng/mL; and 0,21 ng/mL (liver fish) respectively. Metallothionein levels of fish in Kali Jagir river for March, June, and September were 0,26 ng/mL; 0,18 ng/mL; and 0,18 ng/mL (gills fish); 0,2 ng/mL; 0,45 ng/mL; 0,19 ng/mL (liver fish) respectively. We conclude that the damage to the gills and liver of Barbonymus gonionotus was mostly found in Kali Jagir River and the highest metallothionein level of Barbonymus gonionotus also presented in Kali Jagir River.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"40 1","pages":"20-24"},"PeriodicalIF":0.0,"publicationDate":"2017-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72844383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The distribution and composition of Odonata (Dragonfly and Damselfly) in Sriwijaya University, Inderalaya Campus South Sumatera","authors":"Y. Pujiastuti, Yunita Windusari, M. Agus","doi":"10.23869/BPHJBR.23.1.20171","DOIUrl":"https://doi.org/10.23869/BPHJBR.23.1.20171","url":null,"abstract":"","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"32 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75159968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The utilization of link cap mushroom (Coprinus cinereus) on palm oil mill effluent degradation","authors":"Y. B. Subowo","doi":"10.23869/BPHJBR.22.2.20175","DOIUrl":"https://doi.org/10.23869/BPHJBR.22.2.20175","url":null,"abstract":"","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"35 1","pages":"61-67"},"PeriodicalIF":0.0,"publicationDate":"2017-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89972447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Nellen, Atika Hanoum Rahasta, F. Hermanto, M. Lorenza
{"title":"Molecular biology education in Indonesia - suggestions for improvement","authors":"W. Nellen, Atika Hanoum Rahasta, F. Hermanto, M. Lorenza","doi":"10.23869/BPHJBR.22.2.20173","DOIUrl":"https://doi.org/10.23869/BPHJBR.22.2.20173","url":null,"abstract":"","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"10 4","pages":"50-55"},"PeriodicalIF":0.0,"publicationDate":"2017-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72479528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Crab communities (Decapoda: Brachyura) in mangrove and estuaries in the Eastern Part of Lombok Island","authors":"D. Murniati","doi":"10.23869/BPHJBR.22.2.20178","DOIUrl":"https://doi.org/10.23869/BPHJBR.22.2.20178","url":null,"abstract":"Mangroves and estuary ecosystem in East Lombok which are appointed as Local Marine Conservation Areas (Kawasan Konservasi Laut Daerah/KKLD) are Gili Lawang, Gili Sulat, Sambelia and Jerowaru districts. Inventory of aquatic fauna in mangroves and estuaries is one of the important components of the marine conservation program. The objective of this study is to determine crab species composition in mangroves and estuaries of eastern Lombok during the East Lombok KKLD program. Targeted area for this study includes Gili Lawang, Gili Sulat in southern Lombok Island, Sugian in Sambelia, Ekas Beach, Tanjung Luar and Seriwe in Jerowaru, and Kidang in western Jerowaru. The crab was collected at 0 m to 100 m of seashore by digging the burrow. The crab community recorded was analyzed for resulting the Diversity, Evenness and Similarity Index. Ten families with 35 species of crab were found from two ecosystem types, mangrove and estuary. Each location has different diversity of ecosystem and habitat. The highest diversity is recorded in Kidang and Seriwe, while the lowest diversity in Gili Sulat. Species diversity is associated with ecosystem and habitat type diversity. Kidang and Seriwe has the highest ecosystem and habitat diversity, while Gili Sulat has the lowest diversity. In addition, the population of Ocypodidae is accounted as the dominant crab family for which Austruca perplexa is the dominant species.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"43 1","pages":"81-89"},"PeriodicalIF":0.0,"publicationDate":"2017-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86927444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Carbon storage of medium-sized tree: a case study on Dillenia collection in Purwodadi Botanic Garden","authors":"R. Rindyastuti","doi":"10.23869/BPHJBR.22.2.20177","DOIUrl":"https://doi.org/10.23869/BPHJBR.22.2.20177","url":null,"abstract":"Dillenia is a medium-sized tree which has high species diversity in tropical regions especially in Southeast Asia. Dillenia in Purwodadi Botanic Garden are collected from native habitats in Java, Kalimantan, Sulawesi and Papua which planted on the area of 17 x 55 m2. The purpose of this re-search is to study the above ground carbon storage in Dillenia collection in Purwodadi Botanic Garden. Carbon storage estimation was established by measuring stem carbon stocks from plant collections with plant age ranging between 12-30 years. Twelve years old collection contributed carbon storage of 7.35 tonnes/ha for D. sumatrana. Twenty years old species had the lowest carbon storage of 2.17 kg/plant for D. serrata and the highest of 51.9 kg/plant for D. auriculata with a range of carbon storage of 3.47 to 41.072 tonnes/Ha. Thirty years old plant contributed 39.465 kg/plant and carbon storages of 63.14 tonnes/ha for D. serrata and 135.59 kg/plant and 216.94 tonnes/ha for D. philipinensis. Overall, Dillenia collections in Pur-wodadi Botanic Garden contributed 793.94 kg carbon storages, store carbon on average of 30.54 kg/plant and 46.46 tonnes/ha. The increase of carbon storage in the second 10 years was higher than in the first 10 years. It indicated that Dillenia had growth strategy in the early growth then alocated more mass after 10 years. Carbon storage of Dillenia was high and different in age. D. serrata, D. papuana and D. auriculata are recommended species as a priority in planting trees based on carbon sink. Journal of Biological Researches, Vol. 22, No. 2. Hal. 74-80 ISSN 2337-389X","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"86 1","pages":"74-80"},"PeriodicalIF":0.0,"publicationDate":"2017-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83934551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of biocontrol agents isolated from the leaves of mangroves grown in Sampiran Beach, North Sulawesi, Indonesia againts damping-off of cucumber","authors":"Yuliar Yuliar, Suciatmih Suciatmih","doi":"10.23869/BPHJBR.22.2.20176","DOIUrl":"https://doi.org/10.23869/BPHJBR.22.2.20176","url":null,"abstract":"","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"14 1","pages":"68-73"},"PeriodicalIF":0.0,"publicationDate":"2017-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90411424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Potential probiotic evaluation of two Lactobacillus plantarum strains isolated from Indonesian fermented food and fruit","authors":"A. Rosyidah, H. Julistiono","doi":"10.23869/BPHJBR.22.2.20174","DOIUrl":"https://doi.org/10.23869/BPHJBR.22.2.20174","url":null,"abstract":"Two Lactobacillus plantarum strains, InaCC B122 and InaCC B128, isolated from Indonesian fermented food, “dadih” and fruit were evaluated for probiotic potential. These two lactobacilli strains were subjected to a series of in vitro test to assess their surviving capability in gastrointestinal track and antimicrobial activity. The test included acid and bile salt tolerance, adhesion to the yeast Saccharomyces cereviseae cells, heat resistance and antimicrobial activity against Mycobacterium smegmatis, Escherichia coli, and Staphylococcus aureus. Both strains were able to grow under acid and bile salt condition for 24 h. Co-aggregation percentage of strain InaCC B122 and InaCC B128 against the yeast cells was 52.3 % and 53.8 % respectively. Heat treatments (45 °C for 30 min. and 55 °C for 15 min.) did not affect co-aggregation percentages. Their supernatants had antimicrobial activity against M. smegmatis, E. coli and S. aureus with MIC of 5 %, 10 % and 10 % respectively. In conclusion, based on this assessment, these strains are good candidate for probiotic.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"16 1","pages":"56-60"},"PeriodicalIF":0.0,"publicationDate":"2017-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86292042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Hayati, Nureka Tiantono, Muhamad Fadhil Mirza, Iman Dary Supriyadi Putra, Muhamad Maulana Abdizen, Antien Rekyan Seta, Binti Mar`atus Solikha, Muhamad Hilman Fu'adil, T. W. C. Putranto, M. Affandi, Rosmanida Rosmanida
{"title":"Water quality and fish diversity in the Brantas River, East Java, Indonesia:","authors":"A. Hayati, Nureka Tiantono, Muhamad Fadhil Mirza, Iman Dary Supriyadi Putra, Muhamad Maulana Abdizen, Antien Rekyan Seta, Binti Mar`atus Solikha, Muhamad Hilman Fu'adil, T. W. C. Putranto, M. Affandi, Rosmanida Rosmanida","doi":"10.23869/BPHJBR.22.2.20172","DOIUrl":"https://doi.org/10.23869/BPHJBR.22.2.20172","url":null,"abstract":"This research aimed to determine the water quality and fish diversity in Brantas river. Three station for sampling locations were in the upstream (one station on Karangkates reservoir) and downstream (two stations on the Surabaya and Jagir river). Water quality parameters were BOD, COD, DO, pH, temperature, and the levels of heavy metals (Pb, Cr, Cu, and Cd). Fish specimens was identified using fish identification book. The results of this research indicated that there was a difference in the water quality of upstream and downstream of the Brantas river. On the upstream, the source of pollution was mostly from fish catching, agriculture, and industrial activities. While on the downstream of the river it mostly dominated by industrial waste and domestic waste. The water quality in both stations had far exceeded the quality standards that was included in the polluted category. The concluded that Brantas river has been contaminated by waste (heavy metals), exceeded the water quality standard. The diversity of fish in the downstream (Surabaya river) has the highest diversity values, followed Jagir river and in the upstream (Karangkates reservoir) has the lowest value of diversity. Brantas river water was included in the category of moderate diversity.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"55 1","pages":"43-49"},"PeriodicalIF":0.0,"publicationDate":"2017-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73874541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ni’matuzahroh Ni’matuzahroh, I. A. Pratiwi, T. Surtiningsih, F. Fatimah, S. Sumarsih
{"title":"The potency of Micrococcus sp. L II 61 bacteria as oil sludge cleaning agent","authors":"Ni’matuzahroh Ni’matuzahroh, I. A. Pratiwi, T. Surtiningsih, F. Fatimah, S. Sumarsih","doi":"10.23869/BPHJBR.22.2.20171","DOIUrl":"https://doi.org/10.23869/BPHJBR.22.2.20171","url":null,"abstract":"This research aimed to reveal the ability of Micrococcus sp. L II 61 bacteria that was isolated from Pegirian Surabaya as oil cleaning agent. This is an experimental research to detect the presence of biosurfactant and lipase enzyme in culture supernatant of Micrococcus sp. L II 61 with aliphatic hydrocarbon (cooking oil) as a substrate growth. Biosurfactant production was evaluated by measuring the surface tension of supernatant using tensiometer du Nouy and measuring the emulsification activity value using diesel oil as hydrocarbon test. Lipase enzyme was detected by measuring lipolitic activity value of crude enzyme (culture supernatant) by using p-nitrofenil palmitic (p-npp) as a substrate test. Data were analyzed descriptively. The results showed that Micrococcus sp. L II 61 produced biosurfactant with surface tension decreasing of culture supernatant up to 30.27 ± 1.17 mN/m compared than aquadest and value of hydrocarbon emulsification activity (AE 1 hour) up to 20.24 ± 0.68 %. Culture of Micrococcus sp. L II 61 after 16 hours incubation have a lipolytic activity 33.53 ± 0.14 U/mL at pH 7 and 37 C. Supernatant of Micrococcus sp. L II 61 100% (v/v) give the highest percentage of oil sludge solubility, i.e. 86.38 ± 2.39%. Micrococcus sp. L II 61 is a highly potential to be developed as oil sludge cleaning agent.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"29 1","pages":"38-42"},"PeriodicalIF":0.0,"publicationDate":"2017-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79137964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}