{"title":"Morphological and Yield Traits of Pole-Type French Bean Genotypes","authors":"B. Luitel, S. Kalauni, B. Bhandari","doi":"10.3126/JNARC.V7I1.36914","DOIUrl":"https://doi.org/10.3126/JNARC.V7I1.36914","url":null,"abstract":"Understanding the phenotypic variation and association between the grain yield, and other agronomic traits in French bean genotypes is important for its varietal improvement program. Field experiments were conducted during 2018 and 2019 cropping season at Horticulture Research Station, Dailekh, Karnali Province of Nepal to evaluate pole-type French bean genotypes for plant morphological characters, yield and the association between the agronomic traits. Twelve (Bhatte, Chaumase, Dhankute Chhirke, WP Con Bean, White OP, Dhundi Raj, LB-31, LB-37, LB-39, Madhav, Chinese Long, and Trishuli) pole-type French bean genotypes were laid out in a randomized complete block design (RCBD) with three replications. Pole-type French bean genotypes showed the significant variation for plant morphological traits and grain yield. Genotype LB-31 exhibited significantly the highest pod number (78.0/plant), green pod weight (675.0 g/plant), green pod yield (36.1 t/ha), and dry grain yield (2.4 t/ha). Following LB-31 genotype, genotype Chaumase and LB-39 produced the highest green pod yield (31.3 t/ha and 31.2 t/ha, respectively). The number of clusters per plant, pod number per plant and green pod weight per plant exhibited the significant positive correlation with green pod and dry grain yield. Hence, these traits can be selected to improve the yield potential of pole-type French bean genotypes. Pole type French bean genotypes used in the experiment were well adapted to the study area. Therefore, the high yielding genotypes viz LB-31, Chaumase and LB-39 could be recommended to use as seed source for on-farm production in Mid-Western Nepal.","PeriodicalId":31348,"journal":{"name":"Journal of Nepal Agricultural Research Council","volume":"7 1","pages":"10-21"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48159224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Gorkhali, C. Sherpa, M. Kolachhapati, Bhojraj Pokharel, N. Bhattarai, S. Sapkota
{"title":"Intermixing of Commercial Pure Breed Chickens with Indigenous (Sakini) Breed of Nepal","authors":"N. Gorkhali, C. Sherpa, M. Kolachhapati, Bhojraj Pokharel, N. Bhattarai, S. Sapkota","doi":"10.3126/JNARC.V7I1.36927","DOIUrl":"https://doi.org/10.3126/JNARC.V7I1.36927","url":null,"abstract":"The intermixing of pure breeds of commercial chickens with Nepalese indigenous flock of chickens has not been explored yet genetically. The study aims to investigate the status of interbreeding of Nepalese indigenous chickens with exotic breeds. Thirty three samples of indigenous chicken, Sakini, from three different ecological regions, Terai, mid-hill and high hill, were taken for the study. The 522 bp hyper-variable region of D loop mtDNA of each sampled population was PCR amplified and sequenced. The Neighbor joining tree revealed that most of Nepalese Sakini and commercial chickens such as New Hampshire, Rhode Island Red, White Leghorn, White Plymouth Rock and Barred Plymouth Rock lied in same clade. Our study provides first direct evidence that the Sakini was already bred with commercial breeds for the sake of enhancing production performance and also alarms the government to introduce proper strategies and policies to avoid such genetic erosion.","PeriodicalId":31348,"journal":{"name":"Journal of Nepal Agricultural Research Council","volume":"7 1","pages":"92-96"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44625156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Dhakal, T. B. Poon, P. Adhikari, S. Pandey, Shandesh Bhattarai
{"title":"Characterization of Gladiolus Genotypes under Khumaltar Condition of Nepal","authors":"M. Dhakal, T. B. Poon, P. Adhikari, S. Pandey, Shandesh Bhattarai","doi":"10.3126/JNARC.V7I1.36915","DOIUrl":"https://doi.org/10.3126/JNARC.V7I1.36915","url":null,"abstract":"Seven promising genotypes of Gladiolus were experimented during the two consecutive years of 2014/15 and 2015/16 in the field of Horticulture Research Division, Khumaltar, Lalitpur (1332 masl) to evaluate the performances of their vegetative, floral and corm characteristics. The experiment was laid out in Randomized Complete Block Design with four replications. The treatments consisted of seven genotypes (ARSDG-01, ARSDG-02, ARSDG-03, ARSDG-04, ARSDG-05, ARSDG-06 and ARSDG-07). Fifty-four corms of each genotype were planted in the crop geometry of 25 x 25 cm. A total of sixteen characters were observed. The pooled results of two years data indicated significantly different except plant height, spike length, rachis length, number of cormels per mother corm and individual corm weight due to the effects of seven evaluated genotypes. Genotype ARSDG-04 was proven as the earliest one for days to the first spike emergence (73.00 days), the full spike emergence (76.50 days) and the first unfurling of florets (83 days). In contrast, ARSDG-01 and ARSDG-06 were late genotypes for the same three characters. The number of florets/spikes was considerably high in two genotypes viz., ARSDG-06 (21.00) and ARSDG-03 (20.50). The number of daughter corms/mother corm was significantly high in ARSDG-05 (3.10) and ARSDG-04 (2.92) whereas it was minimally low in ARSDG-02 (1.66) and ARSDG-07 (1.69). Summing up all sixteen characters of evaluated genotypes, ARSDG-04 as the first, ARSDG-05 as the second and ARSDG-03 as the third have respectively emerged as superior ones as opposed by those of the rest of evaluated genotypes of Gladiolus.","PeriodicalId":31348,"journal":{"name":"Journal of Nepal Agricultural Research Council","volume":"7 1","pages":"22-29"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45561139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Bhattarai, M. Kamimukai, B. B. Rana, H. Oue, S. Matsumura, M. Murai
{"title":"A Dwarfing Gene sd1-d (Dee-geo-woo-gen dwarf) on Lodging Resistance and Related Traits in Rice","authors":"M. Bhattarai, M. Kamimukai, B. B. Rana, H. Oue, S. Matsumura, M. Murai","doi":"10.3126/JNARC.V7I1.36913","DOIUrl":"https://doi.org/10.3126/JNARC.V7I1.36913","url":null,"abstract":"A dwarfing allele at the sd1 locus on chromosome 1 in rice, sd1-d, has been playing important role for developing lodging-resistant and high-yielding indica varieties IR8 and IR36. The dominant allele SD1 for long culm at the locus is differentiated into SD1-in and SD1-ja that are harbored in indica and japonica subspecies, respectively. The sd1-d of IR36 was substituted with SD1-in or SD1-ja by 17 backcrosses with IR36, and two isogenic tall lines were developed by using an indica variety IR5867 and a japonica one ‘Koshihikari' as donors, which were denoted by “5867-36” and “Koshi-36'', respectively. The present study was conducted to examine the effect of dwarfing gene sd1-d on lodging resistance and related traits, compared with SD1-in and SD1-ja. Two isogenic lines and IR36 were cultivated in the field of the Faculty of Agriculture and Marine Science, Kochi University, Japan during 2017. Regarding index of lodging (g·cm/g × 100), genotypes were in the order: 5867-36 (97.4) g Koshi-36 (74.1) g IR36 (46.0) on the 21st-day after 80%-heading, and they were in the same order on 10th-day after 80%-heading. The 4th-panicle length (cm) was in the order: 5867-36 (118.7) g Koshi-36 (97.6) g IR36 (78.6). Similarly, the 4th-top weight (g) was in the order: 5867-36 (12.2) g Koshi-36 (10.2) g IR36 (9.6). The highest breaking strength (g) was recorded in IR36 (1649) followed by 5867-36 (1493) whereas the lowest breaking strength (g) was recorded in Koshi-36 (1360). Consequently, it is inferred that sd1-d enhances lodging resistance due to the decreases in the length and weight above the 4th-internode as well as the increase of breaking strength. The effect of SD1-in on lodging resistance is lower than that of SD1-ja.","PeriodicalId":31348,"journal":{"name":"Journal of Nepal Agricultural Research Council","volume":"7 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41776401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiban Shrestha, S. Subedi, K. P. Timsina, S. Subedi, M. Pandey, Aakriti, Shrestha, Sajina Shrestha, M. A. Hossain
{"title":"Sustainable Intensification in Agriculture: An Approach for Making Agriculture Greener and Productive","authors":"Jiban Shrestha, S. Subedi, K. P. Timsina, S. Subedi, M. Pandey, Aakriti, Shrestha, Sajina Shrestha, M. A. Hossain","doi":"10.3126/JNARC.V7I1.36937","DOIUrl":"https://doi.org/10.3126/JNARC.V7I1.36937","url":null,"abstract":"Sustainable intensification of agriculture is a good approach for reducing the yield gap without exacerbating the current condition of the environmental components, which is a big challenge for agriculture in the modern world. This review provides a summary of the role and approaches of sustainable intensification in agriculture which offer ways to increase crop production and create long-term sustainability in agriculture production. The current demand for food has continued to rise as a result of the world's rapidly increasing population. In order to increase crop/food production, agricultural systems should be intensified by more sustainable practices, as well as by reforming existing production systems/techniques and diversifying them into newer and more profitable enterprises. Despite the heavy use of inputs, farmers have recently been unable to achieve optimal crop yields. The judicious use of agricultural inputs, combined with improved management techniques, is important for advancing sustainable intensification. New scientific techniques in agronomic practices, as well as improved farm mechanization, are helping to boost resource use efficiency in sustainable crop production. The sustainable agricultural intensification is necessary to increase the agricultural productivity under the changing and adverse climatic conditions while maintaining healthy production practices.","PeriodicalId":31348,"journal":{"name":"Journal of Nepal Agricultural Research Council","volume":"7 1","pages":"133-150"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44850391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biocontrol of Soil Borne Pathogen of Potato Tuber Caused by Rhizoctonia solani through Biosurfactant based Bacillus strain","authors":"Touseef Hussain, A. Khan, M. A. Khan","doi":"10.3126/JNARC.V7I1.36921","DOIUrl":"https://doi.org/10.3126/JNARC.V7I1.36921","url":null,"abstract":"Black scurf disease is a major problem for cultivation of potato worldwide that is managed by synthetic agrochemicals, that are increasing environmental pollution as well as residue in farm produce also.n Therefore, now a day use of bio-agents for managing black scurf is attractive feature and need of the hour. A promising bio surfactant OD02 isolate, was isolated from oil contaminated soil, utilizing its secondary metabolite after preliminary biosurfactant screening tests. Furthermore, this strain exhibited positive test with haemolytic activity, emulsification index, oil spreading test, molecular characterization with 16S rRNA universal primers revealed that the strain belongs to Bacillus group, that was further chemically characterized using TLC, exhibiting reddish pink colour as lipopeptide and demonstrated antagonistic activity against Rhizoctonia solani under in vitro using dual culture (45±0.30%) and at 3% concentration under food poisoning method. Bacillus subtilis HussainT-AMU strain is very effective against phytopathogen R. solani and can be more explored in future diseases management strategies.","PeriodicalId":31348,"journal":{"name":"Journal of Nepal Agricultural Research Council","volume":"7 1","pages":"54-66"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42710488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Insecticides for the Management of Litchi Fruit and Shoot Borer","authors":"S. Upadhyay, S. Aryal, B. Bhusal, B. Chaudhary","doi":"10.3126/jnarc.v6i0.28119","DOIUrl":"https://doi.org/10.3126/jnarc.v6i0.28119","url":null,"abstract":"Litchi is an important subtropical evergreen fruit crop grown in Nepal, which have high nutritive value and refreshing taste. The Litchi growers are experiencing severe loss every year with the damage caused by several insect pests. Among them fruit and shoot borer Conopomorpha sinensis Bradley (Lepidoptera: Gracillariidae) is the one of the important insect pest. Experiments were conducted at RARS Tarahara on existing litchi orchard in RCBD design with four replication and five insecticides (Azadiractin, Chlorantraniliprole, Flubendiamide, Lambda cyhalothrin, and Dimethoate) with recommended doses in litchi orchard against C. sinensis during 2015 and 2016 AD to find out the efficient one. Among the tested insecticide chlorantraniliprole (18.5% w/w SC) and flubendiamide (39.35% m/m SC) each were found to be most efficient against C. sinensis and could be applied at the rate of 1 ml per 3 liter of water when fruit size was about pea and applied 3 times with 10 days interval. nThis result suggested that these two insecticides could be used for the management for C. sinensis. Farmers may reduce the losses and increase their income with the application of these insecticides","PeriodicalId":31348,"journal":{"name":"Journal of Nepal Agricultural Research Council","volume":"6 1","pages":"85-91"},"PeriodicalIF":0.0,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3126/jnarc.v6i0.28119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49052328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Field Screening of Lentil Genotypes against Aphid Infestation in Inner Tarai of Nepal","authors":"S. Neupane, S. Subedi, R. Darai","doi":"10.3126/jnarc.v6i0.28118","DOIUrl":"https://doi.org/10.3126/jnarc.v6i0.28118","url":null,"abstract":"Twenty lentil (Lens culinaris Medik) genotypes received from Grain Legumes Research Program, Khajura, Banke were screened for relative tolerance against aphid (Aphis craccivora Koch.) at the research field of National Maize Research Program, Rampur, Chitwan during winter seasons of two consecutive years 2016 and 2017. The design of the experiment was Randomized Complete Block having three replications. The unit plot size was 4m × 1 m with 25cm row to row spacing and continue plant to plant spacing was maintained and net harvested plot was 4 square meters. The recommended dose of fertilizer was 20:40:20 N:P2O5:K2O kg/ha and seed rate 30 kg/ha. Insect data were collected based on aphid population found at apical twigs (10 cm) per plant and scoring was done during flowering and pod formation stage. The grain yield was recorded. All screened genotypes differed significantly (p<0.05) on aphid population and grain yield. Genotypes, ILL 9924, RL 83, ILL 10856, ILL 6458 and RL 67 were less susceptible with higher grain yield. These results have important implications for the development of aphid tolerant high yielding lentil variety in inner Tarai of Nepal.","PeriodicalId":31348,"journal":{"name":"Journal of Nepal Agricultural Research Council","volume":"6 1","pages":"79-84"},"PeriodicalIF":0.0,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3126/jnarc.v6i0.28118","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44355725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Naked Barley Landraces for Agro-morphological Traits","authors":"A. Karkee, K. Ghimire, B. Joshi","doi":"10.3126/jnarc.v6i0.28112","DOIUrl":"https://doi.org/10.3126/jnarc.v6i0.28112","url":null,"abstract":"Naked barley (Hordeum vulgare var. nudum L.) is a traditional, culturally important, climate-resilient winter cereal crop of Nepal. Evaluation of the naked barely genotypes for yield and disease is fundamental for their efficient utilization in plant breeding schemes and effective conservation programs. Therefore, to identify high yielding and yellow rust resistant landraces of naked barley for hilly and mountainous agro-ecosystem, twenty naked barley landraces collected from different locations of Nepal, were evaluated in randomized complete block design (RCBD) with three replications during winter season of 2016 and 2017 at Khumaltar, Lalitpur, Nepal. Combined analysis of variances revealed that NGRC04902 (3.46 t/ha), NGRC00886 (3.28 t/ha), NGRC02309 (3.21 t/ha) and NGRC06026 (3.10 t/ha) were the high yielding landraces and statistically at par with the released variety 'Solu Uwa' (3.15 t/ha). The landraces namely NGRC00837 (ACI Value: 1.86) was found resistant to yellow rust diseases. Landraces NGRC06034 (131.7 days) and NGRC02363 (130.8 days) were found early maturing and NGRC02306 (94.36 cm) was found dwarf landraces among tested genotypes. These landraces having higher yield and better resistance to yellow rust need to be deployed to farmers' field to diversify the varietal options and used in resistant breeding program to improve the productivity of naked barley for Nepalese farmers.","PeriodicalId":31348,"journal":{"name":"Journal of Nepal Agricultural Research Council","volume":"6 1","pages":"34-43"},"PeriodicalIF":0.0,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3126/jnarc.v6i0.28112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44922011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Joshi, N. Gorkhali, N. Pradhan, K. Ghimire, T. Gotame, Prenil Kc, R. Mainali, A. Karkee, R. Paneru
{"title":"Agrobiodiversity and its Conservation in Nepal","authors":"B. Joshi, N. Gorkhali, N. Pradhan, K. Ghimire, T. Gotame, Prenil Kc, R. Mainali, A. Karkee, R. Paneru","doi":"10.3126/jnarc.v6i0.28111","DOIUrl":"https://doi.org/10.3126/jnarc.v6i0.28111","url":null,"abstract":"Nepal is a part of the world’s biodiversity hotspot and ranks the 49th in the world for biodiversity. Agrobiodiversity and its conservation status were studied through literature review, field survey, key informant survey and focus group discussion. Results of field implementation of some good practices and action research were also documented. Among 24,300 total species in the country, 28% are agricultural genetic resources (AGRs), termed as agrobiodiversity. Agrobiodiversity has six components (crops, forages, livestock, aquatic, insects and microorganisms) and four sub-components (domesticated, semi-domesticated, wild relatives and wild edible) in Nepal. Agrobiodiversity on each component exists at agroecosystem, species, variety/breed/biotype/race/strain, genotype and allele levels, within an altitude range from 60 to 5,000 masl. There are 12 agroecosystems supporting 1026 species under crop component, 510 under forage, 35 under livestock, 250 under the aquatic animal, 17 under aquatic plant, 3,500 under insect and 800 under microorganism. An estimated loss of agrobiodiversity is 40%, however, farmers have reported up to 100% loss of AGRs in some areas for a particular species. Conservation of agrobiodiversity has been initiated since 1986. Four strategies namely ex-situ, on-farm, in-situ and breeding have been adopted for conservation and sustainable utilization of AGRs. Eighty good practices including process, methods and actions for managing agrobiodiversity have been in practice and these practices come under five conservation components (sensitization, method and approach, accelerator, value and enabling environment). Within the country, 18,765 accessions of AGRs have been conserved in different kinds of banks. A total of 24,683 accessions of Nepalese crops, forages and microbes have been conserved in different international and foreign genebanks. Some collections are conserved as safety duplication and safety backup in different CGIARs’ banks and World Seed Vault, Korea. Two global databases (GENESYS and EURISCO) have maintained 19,200 Nepalese accessions. Geographical Information System, Climate Analog Tool and biotechnological tools have been applied for better managing AGRs. Many stakeholders need to further concentrate on the conservation and utilization of AGRs. Global marketing of some native AGRs is necessary for sustaining agriculture and attracting young generations as well as conserving them through use.","PeriodicalId":31348,"journal":{"name":"Journal of Nepal Agricultural Research Council","volume":"6 1","pages":"14-33"},"PeriodicalIF":0.0,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3126/jnarc.v6i0.28111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45722351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}