Journal of Basic and Applied Zoology最新文献_第2页

The neuroprotective action of naringenin on oseltamivir (Tamiflu) treated male rats 柚皮素对奥司他韦(达菲)治疗雄性大鼠的神经保护作用

Journal of Basic and Applied Zoology Pub Date : 2016-10-01 DOI: 10.1016/j.jobaz.2016.12.006

Hoda G. Hegazy , Elham H.A. Ali , Hend A. Sabry

{"title":"The neuroprotective action of naringenin on oseltamivir (Tamiflu) treated male rats","authors":"Hoda G. Hegazy , Elham H.A. Ali , Hend A. Sabry","doi":"10.1016/j.jobaz.2016.12.006","DOIUrl":"10.1016/j.jobaz.2016.12.006","url":null,"abstract":"<div><p>The aim of this study is to evaluate the protective action of naringenin (a flavonoid) on the brain functions of oseltamivir treated rats. 24 rats were divided into 4 groups as follows: control, naringenin treated rats (Nar, 50mg/kgbwt/day), oseltamivir treated rats (Tam, 0.75mg/kgbwt twice daily) and naringenin+oseltamivir treated rats (Nar+Tam). All the drugs were received via oral gavage for five days. The animals on the 5th day were trained in Y maze. Then, on the 6th day, rats were decapitated and the brain was excised for determination of total antioxidant capacity (TAC), total oxidant capacity (TOC), total nitric oxide (TNO), Ca ATPase, total cytochrome P450 (CYP450) contents and brain fatty acid binding proteins FABP7. The results showed a significant increase in the TOC, TNO and CYP450 in Tam treated rats while a significant decrease was noticed in TAC, Ca ATPase and FABP7 in the same group in comparison with the control. Nar+Tam treated rats exhibited a significant decrease in TOC, TNO and CYP450 and a significant increase in TAC, Ca ATPase and FABP7 in comparison with Tam treated rats. An improvement in Y maze behavior and all the investigated parameters was noticed in Nar+Tam treated rats as compared with the oseltamivir treated rats. The results suggest that Nar has a neurophysiological and behavioral protective effect on oseltamivir side effects on the brain functions.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"77 ","pages":"Pages 83-90"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.12.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72639240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Protective effect of curcumin and vitamin C each alone and in combination on cisplatin-induced sperm abnormalities in male albino rats 姜黄素和维生素C单用和联用对雄性白化大鼠顺铂诱导的精子异常的保护作用

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.11.001

Sabha Elsayed Elballat

{"title":"Protective effect of curcumin and vitamin C each alone and in combination on cisplatin-induced sperm abnormalities in male albino rats","authors":"Sabha Elsayed Elballat","doi":"10.1016/j.jobaz.2016.11.001","DOIUrl":"10.1016/j.jobaz.2016.11.001","url":null,"abstract":"<div><p>Infertility is a major concern for young men of reproductive age under-going chemotherapy. Unfortunately, chemotherapeutic treatment for neoplastic diseases commonly impairs fertility either temporally or permanently. In general, chemotherapeutic agents in cancer treatments target all rapidly growing healthy cells, such as those of reproductive system in addition to tumor tissues and it therefore results in impairment of spermatogenesis leading to abnormalities in sperm morphology in cancer survivors. Consequently, antioxidants have been shown to protect nonmalignant cells and organs against damage by chemotherapeutic agents. Hence the present study was designed to evaluate the possible ameliorative role of curcumin or vitamin C alone and their combination in alleviating the toxicity of cisplatin on sperm morphology when given to normal albino rats.</p><p>The results of the present investigation concluded that the combination between curcumin and vitamin C in cisplatin treatment afforded the best ameliorative effect on cisplatin induced sperm shape abnormalities. This may be due to the synergistic effect between curcumin and vitamin C as both of them have antioxidant properties which in turn lead to repairing of sperm abnormalities.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 52-59"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.11.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82482886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Hepatoprotective efficacy of gallic acid during Nitrosodiethylamine-induced liver inflammation in Wistar rats 没食子酸对亚硝基二乙胺致Wistar大鼠肝脏炎症的保护作用

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.07.002

Uzma Latief , Hadiya Husain , Devoshree Mukherjee, Riaz Ahmad

{"title":"Hepatoprotective efficacy of gallic acid during Nitrosodiethylamine-induced liver inflammation in Wistar rats","authors":"Uzma Latief , Hadiya Husain , Devoshree Mukherjee, Riaz Ahmad","doi":"10.1016/j.jobaz.2016.07.002","DOIUrl":"10.1016/j.jobaz.2016.07.002","url":null,"abstract":"<div><p>Gallic acid (GA), a popular phenolic acid is found in gallnuts, grapes, pomegranates, tea and oak bark. It possesses anti-cancer, anti-bacterial, anti-depressant, anti-asthmatic and anti-obesity effects. N′-Nitrosodiethylamine (NDEA) is a well-known hepatotoxin, carcinogen and mutagen. In this study, we have examined the hepatoprotective effect of gallic acid against liver inflammation induced by NDEA in Wistar rats. Hepatic damage in the animals was induced by 10mlkg<sup>−1</sup>   b.wtwk<sup>−1</sup>. Animals belonging to control group were administered equal amounts of saline or GA. LPO, SOD and membrane-bound ATPase (Ca<sup>2+</sup>- and Mg<sup>2+</sup>-ATPase) activities were determined in liver homogenate of control and treated rats. Alterations in liver architecture were assessed by H&E and Masson’s trichrome stainings of 5μm thick liver sections. Immunohistochemistry (IHC) was performed to localize the inflammatory marker, Cyclooxygenase-2 (COX-2). Our results demonstrate a significant increase in malondialdehyde, and decrease in SOD and ATPases (Ca<sup>2+</sup>/Mg<sup>2+</sup>) in NDEA-treated rats. Histopathology data showed inflammation, activated HSCs, deposition of collagen, periportal as well as bridging fibrosis in NDEA-treated liver specimens. Immunohistochemistry of NDEA-treated liver sections exhibited COX-2 positive cells. Gallic acid supplement revert the hepatic functioning in rats injured with NDEA probably by inducing Nrf2-mediated antioxidant enzymes and attenuating the inflammatory mediators COX-2 through NF-κB inhibition pathway. Therefore, gallic acid supplement may be a useful promising bioagent in combating liver injury.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 31-41"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.07.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79828946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

In vivo pretreatment of Eudrilus eugeniae powder attenuates β-adrenoceptor toxicity mediated by isoproterenol in rat model 在大鼠模型中，鲜珠粉体内预处理可减弱异丙肾上腺素介导的β-肾上腺素受体毒性

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.02.003

Jaganathan Anitha , Kadarkarai Murugan , Akon Higuchi , Abdullah A. Alarfaj , Murugan A. Munusamy , Giovanni Benelli

{"title":"In vivo pretreatment of Eudrilus eugeniae powder attenuates β-adrenoceptor toxicity mediated by isoproterenol in rat model","authors":"Jaganathan Anitha , Kadarkarai Murugan , Akon Higuchi , Abdullah A. Alarfaj , Murugan A. Munusamy , Giovanni Benelli","doi":"10.1016/j.jobaz.2016.02.003","DOIUrl":"10.1016/j.jobaz.2016.02.003","url":null,"abstract":"<div><p>The present study was designed to discover the potential cardioprotective function of earthworm powder (EWP) extracted from <em>Eudrilus eugeniae</em> on isoproterenol (ISO)-induced myocardial infarction in male Wistar rats. The rats were divided into four groups, with six rats in each group. Certain rats were pretreated with EWP (200mg/kgbwt) (Group III), and a myocardial infarction was then induced by subcutaneous injection of ISO (85mg/kgbwt) (Group II). Oral pretreatment of 200mg/kgbwt of EWP for 28days significantly (<em>p</em>    <!-->bwt) provides a cardioprotective effect by attenuating the blood profile, lipid profile, biochemical levels, and LDH patterns in rats that experienced an ISO-induced myocardial infarction.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 1-6"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.02.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84964021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sperm abnormality toxicity due to cyclosporine A and the ameliorative effect of royal jelly in male rats 环孢素A对雄性大鼠精子异常毒性及蜂王浆的改善作用

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.12.001

Azza M. Gawish , Samya ElFiky , Mary Therase , Amira AbdElraaof , Wagdi Khalil , Karima A. Mohamed

{"title":"Sperm abnormality toxicity due to cyclosporine A and the ameliorative effect of royal jelly in male rats","authors":"Azza M. Gawish , Samya ElFiky , Mary Therase , Amira AbdElraaof , Wagdi Khalil , Karima A. Mohamed","doi":"10.1016/j.jobaz.2016.12.001","DOIUrl":"10.1016/j.jobaz.2016.12.001","url":null,"abstract":"<div><p>The immunosuppressive drug, utilized widely in Egypt, cyclosporine A was studied to evaluate its toxicity in male rats. Animals were divided into a control (untreated), 3 groups treated intraperitoneally with 20, 40 and 60m/kg cyclosporine A for 5, 10 and 15days, respectively and 3 groups treated intraperitoneally with 20, 40 and 60mg/kg of cyclosporine A plus 100mg/kg royal jelly administrated orally. Toxicity evaluation was carried out using two main endpoints: reproductive study (sperm morphology and count abnormalities) and biochemical changes in liver and testis (DNA amounts). The aim of this work is to study the protective role of royal jelly against sperm abnormalities in shape and count, and changes in DNA contents in liver and testis tissue induced in rats when treated by cyclosporine A with different doses (20–40–60mg/kg/day) for 5, 10, and 15days in male rats and how the royal jelly can repair this changes. Our results showed that sperm abnormalities induced by cyclosporine A included deviation from normal shape in head and tail. Abnormal heads contained amorphous head and banana-shaped head, whereas the abnormal tails included divided and coiled tails. It also induced an insignificant effect on the total sperm counts after 5days of injection with the drug combined with royal jelly. DNA contents were determined in rat liver and testis cells to illustrate the mutagenic effect of cyclosporine A and how the royal jelly can modulate this effect. From these results we concluded that cyclosporine A toxicity was dose and time dependent and should be administrated under special precautions and medical supervision. Using of royal jelly in combination with cyclosporine A drug decreased its toxic effect, so it's considered as protector.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 60-73"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.12.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77271756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Curcumin mitigates lithium-induced thyroid dysfunction by modulating antioxidant status, apoptosis and inflammatory cytokines 姜黄素通过调节抗氧化状态、细胞凋亡和炎症细胞因子减轻锂诱导的甲状腺功能障碍

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.10.001

Sanaa M. Abd El-Twab, Manal Abdul-Hamid

{"title":"Curcumin mitigates lithium-induced thyroid dysfunction by modulating antioxidant status, apoptosis and inflammatory cytokines","authors":"Sanaa M. Abd El-Twab, Manal Abdul-Hamid","doi":"10.1016/j.jobaz.2016.10.001","DOIUrl":"10.1016/j.jobaz.2016.10.001","url":null,"abstract":"<div><p>Lithium is an integral drug used in the management of acute mania, unipolar and bipolar depression and prophylaxis of bipolar disorders. It has also been shown to reduce suicidal risk and short term mortality. Few experimental studies have demonstrated the thyroid toxicity caused by lithium as well as the possible protective effect of curcumin. Twenty four male albino rats were divided into three groups; group I (control group), group II received lithium carbonate daily for 6weeks and group III received the same dose of lithium carbonate as group II concomitantly with curcumin for 6weeks. The specimens were prepared for histopathological, immunohistochemical and biochemical examination. Lithium-induced thyroid dysfunction evidenced by the histopathological and immunohistochemical changes represented by detached cells and vacuolated cytoplasm of some follicular cells and highly significant increase in positive immunostained of thyroglobulin and caspase-3 respectively. Moreover, a significant decrease in serum free triiodothyonine (FT3), free thyroxine (FT4) concomitant with significantly increased thyroid stimulating hormone (TSH) and pro-inflammatory cytokines, and thyroid lipid peroxidation (MDA) and nitric oxide (NO) levels. Curcumin counteracted lithium-induced oxidative stress and inflammation as assessed by restoration of the antioxidant defenses and diminishing of pro-inflammatory cytokines and improvements in the degenerative changes of the thyroid gland. In conclusion, the present study provides evidence that curcumin exerts thyroprotective effects against lithium carbonate mediated by its antioxidant, anti-inflammatory and anti-apoptotic effect as indicated by caspase-3. This report also confers that the use of this drug should be justified for long treatment under direct medical supervision.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 7-19"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84311706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Downregulated StAR gene and male reproductive dysfunction caused by nifedipine and ethosuximide 硝苯地平、乙氧基亚胺致StAR基因下调与男性生殖功能障碍

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.12.005

Rasha A. Ebiya , Metwally M. Montaser , Samia M. Darwish

{"title":"Downregulated StAR gene and male reproductive dysfunction caused by nifedipine and ethosuximide","authors":"Rasha A. Ebiya , Metwally M. Montaser , Samia M. Darwish","doi":"10.1016/j.jobaz.2016.12.005","DOIUrl":"10.1016/j.jobaz.2016.12.005","url":null,"abstract":"<div><p>Steroid hormones that are controlled by steroidogenic acute regulatory (StAR) gene regulate sperm production. However, calcium ion is important for male fertility in vasodilation and sperm development. Calcium also serves as a second messenger to control acrosome reaction and sperm motility. Calcium channel-blockers (CCBs) as nifedipine and ethosuximide (used in hypertension and epilepsy treatment) can affect male fertility. However, little is known about the underlying mechanism of the male reproductive dysfunction and their side effects. The present study was designed to address the involvement of CCBs in inducing male infertility. Thirty-six male mice were orally treated by therapeutic dose of nifedipine and ethosuximide for 20days followed by 10days without treatment for drug withdrawal. Chromosome aberrations assay, sperm analysis and testicular expression level of biomarker steroidogenic acute regulatory (StAR (mRNA were measured. In addition, histological structure of the testis was investigated to the process of spermatogenesis. Our results revealed that, CCBs significantly increased the percentage of chromosome aberration and sperm shape change. StAR-mRNA expression was significantly down regulated. Sperm count and motility were significantly decreased. However, slight improvement was observed in all tested parameters after drug withdrawal. Seminiferous tubules displayed total atrophy, disruption, severe damage and elongation of tubules with disorganization of germinal epithelium that detached from the basement membrane. The lumen of seminiferous tubules showed complete absence of sperm cells.</p></div><div><h3>Conclusions</h3><p>Both nifedipine and ethosuximide significantly increase chromosome abnormalities, decrease sperm function, and down regulate StAR-mRNA expression. All these side effects may lead to irreversible male sterility.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 42-51"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.12.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82083810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Current situation, genetic relationship and control measures of infectious bronchitis virus variants circulating in African regions 非洲地区流行传染性支气管炎病毒变异的现状、遗传关系及控制措施

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.08.002

Khadija Khataby , Amal Souiri , Yassine Kasmi , Chafiqa Loutfi , My Mustapha Ennaji

{"title":"Current situation, genetic relationship and control measures of infectious bronchitis virus variants circulating in African regions","authors":"Khadija Khataby , Amal Souiri , Yassine Kasmi , Chafiqa Loutfi , My Mustapha Ennaji","doi":"10.1016/j.jobaz.2016.08.002","DOIUrl":"10.1016/j.jobaz.2016.08.002","url":null,"abstract":"<div><p>Infectious bronchitis virus (IBV) is a major viral pathogen of commercial poultry, affecting chickens of all ages and causing major economic losses in poultry industry worldwide. Frequent points of mutations and recombination events in the S1 gene region, result in the emergence of new IBVs variants circulating in the form of several serotypes/genotypes that can be partially or poorly neutralized by current vaccines. IBV is well studied worldwide, nevertheless in African countries epidemiological and scientific data are poor and not updated.</p><p>This review aims to give a current overview of IBV situation, to establish evolutionary relationship between the African variants and to list some of the potential measures to control IBV in Africa.</p><p>Three S1 gene hypervariable regions were studied and compared to the reference genotypes/serotypes that found emerging in African regions. This comparison was based on phylogenetic trees, nucleotide and amino-acid sequence analysis. It clearly appears that IBV variants reported in Africa, display a low genetic relationship between them and with the majority of the reference strains emerging in neighboring countries, except the case of variants from Libya and Egypt that show a high relatedness. Also the Massachusetts serotypes were the most prevalent co-circulating with both serotypes, Italy02 type in Morocco and Qx-like genotype in South part of the African continent. In order to control the IBV variants in African regions, an efficient vaccination strategy program should be implemented.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 20-30"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.08.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37832436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Ultra structure differentiation of the anterior pituitary cells of the adult female non pregnant carnivore Vulpes zerda 未怀孕成年雌狐垂体前叶细胞的超微结构分化

Journal of Basic and Applied Zoology Pub Date : 2016-05-01 DOI: 10.1016/j.jobaz.2016.12.003

Atteyat Selim, Eman El Nahass

{"title":"Ultra structure differentiation of the anterior pituitary cells of the adult female non pregnant carnivore Vulpes zerda","authors":"Atteyat Selim, Eman El Nahass","doi":"10.1016/j.jobaz.2016.12.003","DOIUrl":"10.1016/j.jobaz.2016.12.003","url":null,"abstract":"<div><p>The pituitary gland of carnivore mammals in the world was described, but that of those in Egypt may be low, so the present study is carried out on some carnivore mammals such as <em>Vulpes zerda,</em> to elucidate the similarities and the differences of the pituitary cells between the mammals in the world and those in Egypt. The results indicate that, the gland is pyramidal in shape. The acidophilic cells and the basophilic cells are distributed heterogeneously in the body of the gland. The STH cells are numerous, the nucleus is irregular, lobed and eccentric with the endoplasmic reticulum. The secretory granules are light with a large size. The LTH cells have eccentric nucleus near the plasma membrane, the mitochondrium is spherical or elongated, the endoplasmic reticulum is rough and the granules are collected at the cell periphery.</p><p>The ACTH cells are found singly, irregular with eccentric nucleus. Its secretory granules are small and spherical shaped, while the TSH cells have very small secretory granules, but the FSH and LH cells are found singly, angular shape with eccentric nuclei and the its secretary granules are spherical or ovoid shaped and exhibit variation in electron density than STH cells. The differences in shape and distribution may be related to the phylogeny.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"75 ","pages":"Pages 61-67"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.12.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83888659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of caffeine and retinoic acid on skeleton of mice embryos 咖啡因和维甲酸对小鼠胚胎骨骼的影响

Journal of Basic and Applied Zoology Pub Date : 2016-05-01 DOI: 10.1016/j.jobaz.2016.06.003

Fakhr El-Din M. Lashein, Amin A. Seleem, Abeer A. Ahmed

{"title":"Effect of caffeine and retinoic acid on skeleton of mice embryos","authors":"Fakhr El-Din M. Lashein, Amin A. Seleem, Abeer A. Ahmed","doi":"10.1016/j.jobaz.2016.06.003","DOIUrl":"10.1016/j.jobaz.2016.06.003","url":null,"abstract":"<div><p>The present study was conducted to evaluate the effect of caffeine and retinoic acid either separately or in combination on the skeleton of the developing embryos of mice. Pregnant females were treated with either caffeine or retinoic acid at the onset of organogenesis (7th day of gestation). At morphological level no abnormalities in either caffeine or retinoic acid in the developing embryos at 14th day of gestation whose mothers’ were administered caffeine (2mg/100g b.w.) or those of the mothers’ treated with retinoic acid up to 4mg/kg b.w. during the onset of the second trimester of pregnancy were observed. However, dose-dependent retinoic acid treatment initiates chondrocyte vacuolation, depression of PAS+ve intracellular inclusions and depression of nuclear fluorescence that were concomitant with downregulation of TGFβ2 expression in the perichondrium of the developing vertebrae. Co-administration of caffeine was found to ameliorate the effects of 2mg/kg b.w. rather than 4mg/kg b.w. of retinoic acid treatment. At the 18th day of gestation the uterine horns appeared normal without any signs of fetoresorption in all treatments. However, the effect of both caffeine (2mg/100g b.w) and retinoic acid at both doses (2, 4mg/kg b.w) in Alizarin Red stain of wholemount revealed minor phalange deformation of the developing limbs either separately or in combined treatments.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"75 ","pages":"Pages 36-45"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.06.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80019184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5