Journal of Basic and Applied Zoology最新文献_第2页

Protective effect of curcumin and vitamin C each alone and in combination on cisplatin-induced sperm abnormalities in male albino rats 姜黄素和维生素C单用和联用对雄性白化大鼠顺铂诱导的精子异常的保护作用

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.11.001

Sabha Elsayed Elballat

{"title":"Protective effect of curcumin and vitamin C each alone and in combination on cisplatin-induced sperm abnormalities in male albino rats","authors":"Sabha Elsayed Elballat","doi":"10.1016/j.jobaz.2016.11.001","DOIUrl":"10.1016/j.jobaz.2016.11.001","url":null,"abstract":"<div><p>Infertility is a major concern for young men of reproductive age under-going chemotherapy. Unfortunately, chemotherapeutic treatment for neoplastic diseases commonly impairs fertility either temporally or permanently. In general, chemotherapeutic agents in cancer treatments target all rapidly growing healthy cells, such as those of reproductive system in addition to tumor tissues and it therefore results in impairment of spermatogenesis leading to abnormalities in sperm morphology in cancer survivors. Consequently, antioxidants have been shown to protect nonmalignant cells and organs against damage by chemotherapeutic agents. Hence the present study was designed to evaluate the possible ameliorative role of curcumin or vitamin C alone and their combination in alleviating the toxicity of cisplatin on sperm morphology when given to normal albino rats.</p><p>The results of the present investigation concluded that the combination between curcumin and vitamin C in cisplatin treatment afforded the best ameliorative effect on cisplatin induced sperm shape abnormalities. This may be due to the synergistic effect between curcumin and vitamin C as both of them have antioxidant properties which in turn lead to repairing of sperm abnormalities.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 52-59"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.11.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82482886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Hepatoprotective efficacy of gallic acid during Nitrosodiethylamine-induced liver inflammation in Wistar rats 没食子酸对亚硝基二乙胺致Wistar大鼠肝脏炎症的保护作用

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.07.002

Uzma Latief , Hadiya Husain , Devoshree Mukherjee, Riaz Ahmad

{"title":"Hepatoprotective efficacy of gallic acid during Nitrosodiethylamine-induced liver inflammation in Wistar rats","authors":"Uzma Latief , Hadiya Husain , Devoshree Mukherjee, Riaz Ahmad","doi":"10.1016/j.jobaz.2016.07.002","DOIUrl":"10.1016/j.jobaz.2016.07.002","url":null,"abstract":"<div><p>Gallic acid (GA), a popular phenolic acid is found in gallnuts, grapes, pomegranates, tea and oak bark. It possesses anti-cancer, anti-bacterial, anti-depressant, anti-asthmatic and anti-obesity effects. N′-Nitrosodiethylamine (NDEA) is a well-known hepatotoxin, carcinogen and mutagen. In this study, we have examined the hepatoprotective effect of gallic acid against liver inflammation induced by NDEA in Wistar rats. Hepatic damage in the animals was induced by 10mlkg<sup>−1</sup>   b.wtwk<sup>−1</sup>. Animals belonging to control group were administered equal amounts of saline or GA. LPO, SOD and membrane-bound ATPase (Ca<sup>2+</sup>- and Mg<sup>2+</sup>-ATPase) activities were determined in liver homogenate of control and treated rats. Alterations in liver architecture were assessed by H&E and Masson’s trichrome stainings of 5μm thick liver sections. Immunohistochemistry (IHC) was performed to localize the inflammatory marker, Cyclooxygenase-2 (COX-2). Our results demonstrate a significant increase in malondialdehyde, and decrease in SOD and ATPases (Ca<sup>2+</sup>/Mg<sup>2+</sup>) in NDEA-treated rats. Histopathology data showed inflammation, activated HSCs, deposition of collagen, periportal as well as bridging fibrosis in NDEA-treated liver specimens. Immunohistochemistry of NDEA-treated liver sections exhibited COX-2 positive cells. Gallic acid supplement revert the hepatic functioning in rats injured with NDEA probably by inducing Nrf2-mediated antioxidant enzymes and attenuating the inflammatory mediators COX-2 through NF-κB inhibition pathway. Therefore, gallic acid supplement may be a useful promising bioagent in combating liver injury.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 31-41"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.07.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79828946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Sperm abnormality toxicity due to cyclosporine A and the ameliorative effect of royal jelly in male rats 环孢素A对雄性大鼠精子异常毒性及蜂王浆的改善作用

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.12.001

Azza M. Gawish , Samya ElFiky , Mary Therase , Amira AbdElraaof , Wagdi Khalil , Karima A. Mohamed

{"title":"Sperm abnormality toxicity due to cyclosporine A and the ameliorative effect of royal jelly in male rats","authors":"Azza M. Gawish , Samya ElFiky , Mary Therase , Amira AbdElraaof , Wagdi Khalil , Karima A. Mohamed","doi":"10.1016/j.jobaz.2016.12.001","DOIUrl":"10.1016/j.jobaz.2016.12.001","url":null,"abstract":"<div><p>The immunosuppressive drug, utilized widely in Egypt, cyclosporine A was studied to evaluate its toxicity in male rats. Animals were divided into a control (untreated), 3 groups treated intraperitoneally with 20, 40 and 60m/kg cyclosporine A for 5, 10 and 15days, respectively and 3 groups treated intraperitoneally with 20, 40 and 60mg/kg of cyclosporine A plus 100mg/kg royal jelly administrated orally. Toxicity evaluation was carried out using two main endpoints: reproductive study (sperm morphology and count abnormalities) and biochemical changes in liver and testis (DNA amounts). The aim of this work is to study the protective role of royal jelly against sperm abnormalities in shape and count, and changes in DNA contents in liver and testis tissue induced in rats when treated by cyclosporine A with different doses (20–40–60mg/kg/day) for 5, 10, and 15days in male rats and how the royal jelly can repair this changes. Our results showed that sperm abnormalities induced by cyclosporine A included deviation from normal shape in head and tail. Abnormal heads contained amorphous head and banana-shaped head, whereas the abnormal tails included divided and coiled tails. It also induced an insignificant effect on the total sperm counts after 5days of injection with the drug combined with royal jelly. DNA contents were determined in rat liver and testis cells to illustrate the mutagenic effect of cyclosporine A and how the royal jelly can modulate this effect. From these results we concluded that cyclosporine A toxicity was dose and time dependent and should be administrated under special precautions and medical supervision. Using of royal jelly in combination with cyclosporine A drug decreased its toxic effect, so it's considered as protector.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 60-73"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.12.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77271756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Curcumin mitigates lithium-induced thyroid dysfunction by modulating antioxidant status, apoptosis and inflammatory cytokines 姜黄素通过调节抗氧化状态、细胞凋亡和炎症细胞因子减轻锂诱导的甲状腺功能障碍

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.10.001

Sanaa M. Abd El-Twab, Manal Abdul-Hamid

{"title":"Curcumin mitigates lithium-induced thyroid dysfunction by modulating antioxidant status, apoptosis and inflammatory cytokines","authors":"Sanaa M. Abd El-Twab, Manal Abdul-Hamid","doi":"10.1016/j.jobaz.2016.10.001","DOIUrl":"10.1016/j.jobaz.2016.10.001","url":null,"abstract":"<div><p>Lithium is an integral drug used in the management of acute mania, unipolar and bipolar depression and prophylaxis of bipolar disorders. It has also been shown to reduce suicidal risk and short term mortality. Few experimental studies have demonstrated the thyroid toxicity caused by lithium as well as the possible protective effect of curcumin. Twenty four male albino rats were divided into three groups; group I (control group), group II received lithium carbonate daily for 6weeks and group III received the same dose of lithium carbonate as group II concomitantly with curcumin for 6weeks. The specimens were prepared for histopathological, immunohistochemical and biochemical examination. Lithium-induced thyroid dysfunction evidenced by the histopathological and immunohistochemical changes represented by detached cells and vacuolated cytoplasm of some follicular cells and highly significant increase in positive immunostained of thyroglobulin and caspase-3 respectively. Moreover, a significant decrease in serum free triiodothyonine (FT3), free thyroxine (FT4) concomitant with significantly increased thyroid stimulating hormone (TSH) and pro-inflammatory cytokines, and thyroid lipid peroxidation (MDA) and nitric oxide (NO) levels. Curcumin counteracted lithium-induced oxidative stress and inflammation as assessed by restoration of the antioxidant defenses and diminishing of pro-inflammatory cytokines and improvements in the degenerative changes of the thyroid gland. In conclusion, the present study provides evidence that curcumin exerts thyroprotective effects against lithium carbonate mediated by its antioxidant, anti-inflammatory and anti-apoptotic effect as indicated by caspase-3. This report also confers that the use of this drug should be justified for long treatment under direct medical supervision.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 7-19"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84311706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Downregulated StAR gene and male reproductive dysfunction caused by nifedipine and ethosuximide 硝苯地平、乙氧基亚胺致StAR基因下调与男性生殖功能障碍

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.12.005

Rasha A. Ebiya , Metwally M. Montaser , Samia M. Darwish

{"title":"Downregulated StAR gene and male reproductive dysfunction caused by nifedipine and ethosuximide","authors":"Rasha A. Ebiya , Metwally M. Montaser , Samia M. Darwish","doi":"10.1016/j.jobaz.2016.12.005","DOIUrl":"10.1016/j.jobaz.2016.12.005","url":null,"abstract":"<div><p>Steroid hormones that are controlled by steroidogenic acute regulatory (StAR) gene regulate sperm production. However, calcium ion is important for male fertility in vasodilation and sperm development. Calcium also serves as a second messenger to control acrosome reaction and sperm motility. Calcium channel-blockers (CCBs) as nifedipine and ethosuximide (used in hypertension and epilepsy treatment) can affect male fertility. However, little is known about the underlying mechanism of the male reproductive dysfunction and their side effects. The present study was designed to address the involvement of CCBs in inducing male infertility. Thirty-six male mice were orally treated by therapeutic dose of nifedipine and ethosuximide for 20days followed by 10days without treatment for drug withdrawal. Chromosome aberrations assay, sperm analysis and testicular expression level of biomarker steroidogenic acute regulatory (StAR (mRNA were measured. In addition, histological structure of the testis was investigated to the process of spermatogenesis. Our results revealed that, CCBs significantly increased the percentage of chromosome aberration and sperm shape change. StAR-mRNA expression was significantly down regulated. Sperm count and motility were significantly decreased. However, slight improvement was observed in all tested parameters after drug withdrawal. Seminiferous tubules displayed total atrophy, disruption, severe damage and elongation of tubules with disorganization of germinal epithelium that detached from the basement membrane. The lumen of seminiferous tubules showed complete absence of sperm cells.</p></div><div><h3>Conclusions</h3><p>Both nifedipine and ethosuximide significantly increase chromosome abnormalities, decrease sperm function, and down regulate StAR-mRNA expression. All these side effects may lead to irreversible male sterility.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 42-51"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.12.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82083810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Current situation, genetic relationship and control measures of infectious bronchitis virus variants circulating in African regions 非洲地区流行传染性支气管炎病毒变异的现状、遗传关系及控制措施

Journal of Basic and Applied Zoology Pub Date : 2016-08-01 DOI: 10.1016/j.jobaz.2016.08.002

Khadija Khataby , Amal Souiri , Yassine Kasmi , Chafiqa Loutfi , My Mustapha Ennaji

{"title":"Current situation, genetic relationship and control measures of infectious bronchitis virus variants circulating in African regions","authors":"Khadija Khataby , Amal Souiri , Yassine Kasmi , Chafiqa Loutfi , My Mustapha Ennaji","doi":"10.1016/j.jobaz.2016.08.002","DOIUrl":"10.1016/j.jobaz.2016.08.002","url":null,"abstract":"<div><p>Infectious bronchitis virus (IBV) is a major viral pathogen of commercial poultry, affecting chickens of all ages and causing major economic losses in poultry industry worldwide. Frequent points of mutations and recombination events in the S1 gene region, result in the emergence of new IBVs variants circulating in the form of several serotypes/genotypes that can be partially or poorly neutralized by current vaccines. IBV is well studied worldwide, nevertheless in African countries epidemiological and scientific data are poor and not updated.</p><p>This review aims to give a current overview of IBV situation, to establish evolutionary relationship between the African variants and to list some of the potential measures to control IBV in Africa.</p><p>Three S1 gene hypervariable regions were studied and compared to the reference genotypes/serotypes that found emerging in African regions. This comparison was based on phylogenetic trees, nucleotide and amino-acid sequence analysis. It clearly appears that IBV variants reported in Africa, display a low genetic relationship between them and with the majority of the reference strains emerging in neighboring countries, except the case of variants from Libya and Egypt that show a high relatedness. Also the Massachusetts serotypes were the most prevalent co-circulating with both serotypes, Italy02 type in Morocco and Qx-like genotype in South part of the African continent. In order to control the IBV variants in African regions, an efficient vaccination strategy program should be implemented.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"76 ","pages":"Pages 20-30"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.08.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37832436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Ultra structure differentiation of the anterior pituitary cells of the adult female non pregnant carnivore Vulpes zerda 未怀孕成年雌狐垂体前叶细胞的超微结构分化

Journal of Basic and Applied Zoology Pub Date : 2016-05-01 DOI: 10.1016/j.jobaz.2016.12.003

Atteyat Selim, Eman El Nahass

{"title":"Ultra structure differentiation of the anterior pituitary cells of the adult female non pregnant carnivore Vulpes zerda","authors":"Atteyat Selim, Eman El Nahass","doi":"10.1016/j.jobaz.2016.12.003","DOIUrl":"10.1016/j.jobaz.2016.12.003","url":null,"abstract":"<div><p>The pituitary gland of carnivore mammals in the world was described, but that of those in Egypt may be low, so the present study is carried out on some carnivore mammals such as <em>Vulpes zerda,</em> to elucidate the similarities and the differences of the pituitary cells between the mammals in the world and those in Egypt. The results indicate that, the gland is pyramidal in shape. The acidophilic cells and the basophilic cells are distributed heterogeneously in the body of the gland. The STH cells are numerous, the nucleus is irregular, lobed and eccentric with the endoplasmic reticulum. The secretory granules are light with a large size. The LTH cells have eccentric nucleus near the plasma membrane, the mitochondrium is spherical or elongated, the endoplasmic reticulum is rough and the granules are collected at the cell periphery.</p><p>The ACTH cells are found singly, irregular with eccentric nucleus. Its secretory granules are small and spherical shaped, while the TSH cells have very small secretory granules, but the FSH and LH cells are found singly, angular shape with eccentric nuclei and the its secretary granules are spherical or ovoid shaped and exhibit variation in electron density than STH cells. The differences in shape and distribution may be related to the phylogeny.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"75 ","pages":"Pages 61-67"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.12.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83888659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of caffeine and retinoic acid on skeleton of mice embryos 咖啡因和维甲酸对小鼠胚胎骨骼的影响

Journal of Basic and Applied Zoology Pub Date : 2016-05-01 DOI: 10.1016/j.jobaz.2016.06.003

Fakhr El-Din M. Lashein, Amin A. Seleem, Abeer A. Ahmed

{"title":"Effect of caffeine and retinoic acid on skeleton of mice embryos","authors":"Fakhr El-Din M. Lashein, Amin A. Seleem, Abeer A. Ahmed","doi":"10.1016/j.jobaz.2016.06.003","DOIUrl":"10.1016/j.jobaz.2016.06.003","url":null,"abstract":"<div><p>The present study was conducted to evaluate the effect of caffeine and retinoic acid either separately or in combination on the skeleton of the developing embryos of mice. Pregnant females were treated with either caffeine or retinoic acid at the onset of organogenesis (7th day of gestation). At morphological level no abnormalities in either caffeine or retinoic acid in the developing embryos at 14th day of gestation whose mothers’ were administered caffeine (2mg/100g b.w.) or those of the mothers’ treated with retinoic acid up to 4mg/kg b.w. during the onset of the second trimester of pregnancy were observed. However, dose-dependent retinoic acid treatment initiates chondrocyte vacuolation, depression of PAS+ve intracellular inclusions and depression of nuclear fluorescence that were concomitant with downregulation of TGFβ2 expression in the perichondrium of the developing vertebrae. Co-administration of caffeine was found to ameliorate the effects of 2mg/kg b.w. rather than 4mg/kg b.w. of retinoic acid treatment. At the 18th day of gestation the uterine horns appeared normal without any signs of fetoresorption in all treatments. However, the effect of both caffeine (2mg/100g b.w) and retinoic acid at both doses (2, 4mg/kg b.w) in Alizarin Red stain of wholemount revealed minor phalange deformation of the developing limbs either separately or in combined treatments.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"75 ","pages":"Pages 36-45"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.06.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80019184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Evaluation of the morphological adaptations of the small intestine of the African pied crow (Corvus albus) 非洲斑鸦小肠形态适应性的评价

Journal of Basic and Applied Zoology Pub Date : 2016-05-01 DOI: 10.1016/j.jobaz.2016.12.002

Okpe Chidozie Godwin, Abiaezute Nwabugwu Clifford, Adigwe Agatha

{"title":"Evaluation of the morphological adaptations of the small intestine of the African pied crow (Corvus albus)","authors":"Okpe Chidozie Godwin, Abiaezute Nwabugwu Clifford, Adigwe Agatha","doi":"10.1016/j.jobaz.2016.12.002","DOIUrl":"10.1016/j.jobaz.2016.12.002","url":null,"abstract":"<div><p>The morphology and morphometry of the small intestine of the Adult African pied crow in a tropical habitat was studied. Fifteen African pied crows used in this study revealed modification of the jejunum into centripetal and centrifugal cone shaped spiral coils. The weight and length of jejunum was significantly greater (<em>p</em>  <0.05) higher than those of the duodenum and ileum respectively while the population of goblet cells were significantly higher (<em>p</em>  <0.05) in the jejunum than in the ileum and duodenum respectively. The morphological modifications probably maximize space for efficient absorption and reduction of digestive load.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"75 ","pages":"Pages 54-60"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.12.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90300383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Effect of verapamil on some of the pro- and apoptotic factors during prenatal retinal differentiation of mice, Mus musculus 维拉帕米对小鼠产前视网膜分化过程中促凋亡因子的影响

Journal of Basic and Applied Zoology Pub Date : 2016-05-01 DOI: 10.1016/j.jobaz.2016.05.001

Amin Abdou Seleem, Fakhr El-Din M. Lashein

{"title":"Effect of verapamil on some of the pro- and apoptotic factors during prenatal retinal differentiation of mice, Mus musculus","authors":"Amin Abdou Seleem, Fakhr El-Din M. Lashein","doi":"10.1016/j.jobaz.2016.05.001","DOIUrl":"10.1016/j.jobaz.2016.05.001","url":null,"abstract":"<div><p>Verapamil is a calcium channel blocker that belongs to the phenylalkylamine group. It has been clinically used for various diseases such as combating hypertension, ischemic heart diseases, supraventricular antiarrhythmic and tycolysis. The study was conducted to investigate the effect of verapamil on selected pro- and apoptotic factors during prenatal retinal differentiation of mice at E14 and E17 of gestation. The pregnant females were classified into two groups, the first is the control and the second receives SC injection of repeated doses of verapamil (40mg/kg) at the 7th day of gestation. The pregnant females were sacrificed at E14 and at E17 of pregnancy and their ocular regions were separated. The retina of mentioned ages were examined at histological and immunohistochemistry of Cytochrome C, Caspase-3 as a pro-apoptotic; Bak and TNFα R2 as apoptotic factors that engaged in proper normal development. The present findings revealed that verapamil-treatment exhibited comparative thinning of inner plexiform layer and reduction of nuclear in E14 and missing of the ganglion layer and comparative decrease of nuclear cells of E17 comparing to the control. Also, the expression of Cytochrome C, Caspase-3, Bak and TNFα R2 in the developing retina was obviously inhibited in verapamil-treatment at E17 compared to the control group. The study concluded that verapamil, as a calcium channel blocker, has the ability to alter the histology of the retina and suppress the studied markers resulting in disorganization of the eye during prenatal development.</p></div>","PeriodicalId":31288,"journal":{"name":"Journal of Basic and Applied Zoology","volume":"75 ","pages":"Pages 28-35"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jobaz.2016.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75978180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1