{"title":"Identification and sequencing of bla CTX-M genes in clinical isolates of Klebsiella pneumoniae isolated from Milad hospital","authors":"Samar Sedaghatpishe, M. Ghane, L. Babaeekhou","doi":"10.32592/jbirjandunivmedsci.2019.26.4.103","DOIUrl":"https://doi.org/10.32592/jbirjandunivmedsci.2019.26.4.103","url":null,"abstract":"Background and Aim: Klebsiella pneumoniae is one of the most important causes of nosocomial infection which has recently received much attention due to its antibiotic resistance. The aim of the present study is the identification and sequencing of blaCTX-M genes in clinical isolates of K. pneumonia isolated from Milad Hospital in Tehran. Materials and Methods: In this descriptive cross-sectional study, first, antibiotic resistance of 100 K. pnuemoniae isolates to cephalosporins was performed by agar diffusion method; then blaCTX-M group2 and blaCTX-M group9 resistance genes were identified by PCR. Genotyping was performed based on the sequence of these genes and the dendrogram was drawn using the Mega 6 software (version 6). Results: According to the antibiotic sensitivity testing, the amount of resistance to cephalosporins was between 30 and 54 percent. Overall, 5% of isolates had blaCTX-M group2 and 8% of isolates had blaCTX-M group9 as well as, the genotyping results showed that in this study bla CTX-M group2 sequence with the sequences in the global database (NCBI) had little similarity, and the blaCTX-M group9 gene sequence was similar to the bla CTX-M-14 sequence gene of E. coli. Conclusion: However, the frequency of blaCTX-M genes was low in this study, but due to the ability of these genes to spread by mobile genetic elements among enterobacteriaceae, it is considered alarm in the development of drug resistance among K. pneumoniae.","PeriodicalId":31015,"journal":{"name":"Journal of Birjand University of Medical Sciences","volume":"26 1","pages":"315-326"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49552129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Somaye Fakhri, Saeed Shakeryan, Fatemeh Fakhri, Ali Akbar Alizadeh
{"title":"The effect of 6 weeks of high-intensity interval training (HIIT) with using Nanocurcumin supplement on total antioxidant capacity and Malondialdehyde level in overweight girls","authors":"Somaye Fakhri, Saeed Shakeryan, Fatemeh Fakhri, Ali Akbar Alizadeh","doi":"10.32592/jbirjandunivmedsci.2019.26.4.105","DOIUrl":"https://doi.org/10.32592/jbirjandunivmedsci.2019.26.4.105","url":null,"abstract":"Background and Aim: Despite the positive effects of high-intensity interval training (HIIT), these training cause oxidative stress due to high intensity. Therefore, the purpose of this study was to investigate the effect of 6 weeks of HIIT training by using NanoCurcumin supplement on total antioxidant capacity and Malondialdehyde level in overweight girls. Materials and Methods: The present study was a quasi-experimental study with a pretest-posttest design. Accordingly 48 overweight girl students with average age (21.78 ± 0.94 years) and BMI (28.12±2.1 Kg/m2) were entered randomly and equal divided into four groups: training group (n=12), training-supplement (n=12), supplement (n=12) and control group (n=12). Supplemental groups consumed an 80 mg capsule of Nano-Curcumin daily. Training groups, three sessions per week for 6 weeks performed HIIT exercises with 85 to 95% of maximum heart rate. Before and after training and consumption the supplementation, Blood samples were obtained to measure Malondialdehyde indices and total antioxidant capacity. Results: In the present study, there was a significant decrease in Malondialdehyde levels (P = 0.009) and a significant increase in total antioxidant capacity (P=0.01) in the training-supplement group while levels of Malondialdehyde increased significantly (P = 0.004) in the training group. Conclusion: It seems, intense HIIT training causes oxidative stress in overweight people, concomitant use of the supplement may possibly improve the total antioxidant capacity in overweight people.","PeriodicalId":31015,"journal":{"name":"Journal of Birjand University of Medical Sciences","volume":"26 1","pages":"333-342"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45203238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aazam Yousefi, Gholamreza Sharifzadeh, Ensiyeh Norozi, S. Sahranavard, M. Miri
{"title":"The investigation adjustment status post-divorce and related factors in divorced women supported by the Birjand-based Relief Committee in 2018","authors":"Aazam Yousefi, Gholamreza Sharifzadeh, Ensiyeh Norozi, S. Sahranavard, M. Miri","doi":"10.32592/jbirjandunivmedsci.2019.26.4.107","DOIUrl":"https://doi.org/10.32592/jbirjandunivmedsci.2019.26.4.107","url":null,"abstract":"Background and Aim: Given the increasing divorce rate and vulnerability of divorced women in society, this study was aimed at the investigation adjustment status post-divorce and related factors in divorced women supported by the Birjand-based Relief Committee. Materials and Methods: This descriptive-analytical study was performed on 160 divorced women covered by the Birjand Relief Committee by a multi-stage sampling method in 2018. A demographic questionnaire and the Fisher’s Divorce adjustment Scale were used for data collection. Data were analyzed by SPSS software (version19) and analyzed by independent t-test, one-way ANOVA, and Tukey post-hoc tests at α = 0.05. Results: The mean age of participants was 37.5± 6.2 years and the main cause of divorce in women was addiction of the spouse (44.4%). The total mean score of adjustment in women was 331.1± 56.3, where 62.7% had poor adjustment and 37.3% had moderate adjustment. The mean score of adjustment in employed women was significantly higher than in household women (p <0.001) and the adjustment score increased significantly as the education level increased (p=0.027). The results also showed that there was no significant relationship between age, number of child, ex-spouse occupation, duration of the marriage, and years after divorce with post-divorce adjustment. Conclusion: The results of the present study showed that divorced women, especially housewives and low-educated women, do not have a good adaptation status, therefore perform the interventions needed to improve the compatibility status it is suggested to officials and planners.","PeriodicalId":31015,"journal":{"name":"Journal of Birjand University of Medical Sciences","volume":"26 1","pages":"353-362"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45243450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. B. Esfeden, A. Ghaderi, A. Dashtgard, Marzie Moghanni
{"title":"Antibiotic resistance pattern and identification of Extended -Spectrum BetaLactamase producing Gram-Negative Bacteria obtained from patients admitted in educational hospitals of Shohada Qaen","authors":"Z. B. Esfeden, A. Ghaderi, A. Dashtgard, Marzie Moghanni","doi":"10.32592/jbirjandunivmedsci.2019.26.4.108","DOIUrl":"https://doi.org/10.32592/jbirjandunivmedsci.2019.26.4.108","url":null,"abstract":"Background and Aim: Determination of antibiotic resistance pattern and awareness of current resistance in each region, it can help to take appropriate therapeutic measures. The purpose of this study to identification of bacterial agents causing infection, and was the determination of their antibiotic resistance in patients admitted to educational hospital of Shohada Qaen, through the years 20182019. Materials and Methods: In this descriptive-analytic study, in the period of one year, 1980 samples were collected from patients admitted to educational hospitals of Shohada Qaen. The specimens were cultured in blood agar and EMB, and were incubated at 37 ° C for 24 hours, then the infection-causing bacteria were identified by differential biochemical tests, depending on gram positive or gram negative bacteria. The Disk diffusion method was applied to determine antibiotic resistance pattern, and the combined disk phenotypic method was applied to determine the strains producing e extended -spectrum beta-lactamase enzymes (ESBLs). Results: of 1980 samples collected from patients admitted, 183 bacterial isolates were collected from which 151 gram negative and 32 gram-positive bacteria were identified. The most common isolated bacteria were Escherichia coli with a frequency of 60.1%, followed by Staphylococcus aureus and Klebsiella, with a frequency of 11.5% and 10.9% respectively. Generally, gram-positive bacteria had the least resistance to Ciprofloxacin and Imipenem and gram-negative bacteria had the least resistance to Amikacin. 44.7% of the gram-negative bacteria produced ESBL. Conclusion: Antibiotic administration based on the antibiotic resistance pattern can be more effective and useful. The high prevalence of ESBL producing strains indicates the necessity of rapid monitoring and identification of these strains.","PeriodicalId":31015,"journal":{"name":"Journal of Birjand University of Medical Sciences","volume":"26 1","pages":"363-371"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46765381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seyyed Gholamreza Mortazavi Moghaddam, Hosein Gazi
{"title":"Relationship between oxygen saturation percentage and forced expiratory volume in first second in chronic obstructive pulmonary disease","authors":"Seyyed Gholamreza Mortazavi Moghaddam, Hosein Gazi","doi":"10.32592/jbirjandunivmedsci.2019.26.4.104","DOIUrl":"https://doi.org/10.32592/jbirjandunivmedsci.2019.26.4.104","url":null,"abstract":"Background and Aim: Considering the cost and some limitations in measuring the percentage of expiratory volume in the first second (FEV1%), This study was performed to determine the relationship between oxygen saturation percentage (SPO2%) with expiratory volume in the first-second (% FEV1) in patients with chronic obstructive pulmonary disease(COPD). Materials and Methods: In a descriptive-analytical study, 25 women and 25 men with COPD were selected by Non-probability and simple sampling from patients referred to the outpatient clinic of Vali-e-Asr Hospital regardless of the severity of the disease. To measure of % FEV1, spirometry method and a finger pulse oximeter to measure SPO2 was used.Statistical tests including Pearson test and independent t-test and linear regression model were used. Results: The mean patient age 63.18±10.47 years, average % FEV1 of 36.60 ± 16.15 and the mean SPO2% 84.20±5.35 was respectively. The correlation coefficient between% FEV1 and SPO2% was 0.36 (P=0.01). Mean of SPO2% in smokers and nonsmoker was %82.67±5.22 and %86.50±5.29 respectively (p=0.02). Regression analysis in constant conditions showed %FEV1 By increasing one unit SPO2%, the amount of FEV1% with probability factor of 0.16 will be increased 0.36(P=0.02). Conclusion: The amount of SPO2% significant correlation at the medium level with the amount of %FEV1 and can predict to some extent FEV1%; Therefore, it has been diagnosed in people with obstructive pulmonary disease and it can be used for evaluation and follow-up of patients instead of spirometry.","PeriodicalId":31015,"journal":{"name":"Journal of Birjand University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45642803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effect of hydroalcoholic extract of Mentha Pulegium on anxiety in male rats","authors":"Fatemeh Veisi Hampa, M. Sofiabadi","doi":"10.32592/jbirjandunivmedsci.2019.26.4.101","DOIUrl":"https://doi.org/10.32592/jbirjandunivmedsci.2019.26.4.101","url":null,"abstract":"Background and Aim: Anxiety is one of the most common mental disorders that decrease the quality of life and leads to chronic disorders. Studies have shown that the Mentha Pulegium extract can affect the central nervous system. Due to the side effects of most anxiolytic chemical drugs; this study was conducted to evaluate the effect of Mentha Pulegium hydroalcoholic extract leaves on anxiety in male rats. Materials and Methods: In this experimental study, 24 male rats were used. Animals were divided into 4 groups (N=6), including control group (untreated) and three experimental groups receiving doses of 400, 800, 1600 mg/kg (rat weight) of Mentha Pulegium hydroalcoholic extract. The extract was injected intraperitoneally half an hour before the test. Anxiety was assessed by using elevated plus maze. The duration and frequency of the presence of the rats in open and closed arms of maze were recorded. Data were analyzed by one-way ANOVA and Tukey post hoc tests. Results: The mean percentage of number of entrance open arms and their duration was significantly increased in rat receiving the extract compare than control (P<0.05), and the duration of presence in closed arm was reduced by the extract (P<0.05, P <0.01). Conclusion: According to these findings, Mentha Pulegium hydroalcoholic extract leaves reduces anxiety in rats by dose-dependent manner.","PeriodicalId":31015,"journal":{"name":"Journal of Birjand University of Medical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69705256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khadijeh Farrokhfall, Z. F. Hassanabad, Zahra Gholamnejad
{"title":"Vascular effects of aqueous Crocus sativus petals’ extract in the hypertensive\u0000rats","authors":"Khadijeh Farrokhfall, Z. F. Hassanabad, Zahra Gholamnejad","doi":"10.32592/jbirjandunivmedsci.2020.27.3.105","DOIUrl":"https://doi.org/10.32592/jbirjandunivmedsci.2020.27.3.105","url":null,"abstract":"Background and Aim: Crocus sativus (C. sativus) petals attenuates smooth muscle tension and blood pressure in control animals. However the antihypertensive effect and its mechanisms haven’t been recognized. This study investigates the antihypertensive effects of C. sativus petals’ aqueous extract in hypertensive rats and also responses of the rat isolated perfused mesenteric bed.\u0000Materials and Methods: The interventional experimental study was performed on 20 male rats (divided to 5 and 15 rats for in vivo and in vitro studies respectively). Hypertension was induced by DOCA-salt injection (20 mg/kg, twice weekly, for 5 weeks, S.C) and water was replaced by NaCl (1%). Five weeks later, animals were anaesthetized with sodium thiopental (30 mg intraperitoneal). Then systemic arterial blood pressure was measured by cannulation of carotid artery following administration of different doses of aqueous extract of C. sativus. Isolated mesenteric beds precontracted with KCl (40 mM), and the tension was measured in presence of different concentrations of the aqueous extract. Finally, various doses of C. sativus extract were applied after incubation by L-NAME or indomethacin. The mesentry was perfused with pump and the recordings were done by physiograph.\u0000Results: Mean arterial blood pressure in hypertensive rats was 231±6 mmHg. Administration of aqueous extracts of C. sativus reduced the blood pressure in a dose–dependent manner. In mesenteric beds preparation, addition of C. sativus reduced the contractile effects of KCl. Incubation with L-NAME but not indomethacin abolished hypotension effect of the extract.\u0000Conclusion: It was proposed that the antihypertensive effects of C. sativus petals’ extract are through the reduction in total peripheral resistance following nitric oxide production.","PeriodicalId":31015,"journal":{"name":"Journal of Birjand University of Medical Sciences","volume":"16 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90765463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}