Medicinal Plant Biology最新文献

Synergistic effects of exogenous inducers on ganoderic acid accumulation in Ganoderma lucidum: optimization and transcriptomic analysis 外源诱导剂对灵芝果实中灵芝酸积累的协同效应：优化及转录组学分析

Medicinal Plant Biology Pub Date : 2026-01-01 DOI: 10.48130/mpb-0025-0039

Yanhong Guo, Shuhao Xu, Xue Guo, Jing Xu, Zhen Li, Ying Wang, Zhenhao Li, Zheyong Xue

{"title":"Synergistic effects of exogenous inducers on ganoderic acid accumulation in Ganoderma lucidum: optimization and transcriptomic analysis","authors":"Yanhong Guo, Shuhao Xu, Xue Guo, Jing Xu, Zhen Li, Ying Wang, Zhenhao Li, Zheyong Xue","doi":"10.48130/mpb-0025-0039","DOIUrl":"https://doi.org/10.48130/mpb-0025-0039","url":null,"abstract":"Ganoderic acids (GAs) are bioactive compounds in Ganoderma lucidum that have various pharmacological properties. Their biosynthesis is influenced by environmental factors and elicitors, but the synergistic effects of multiple inducers on GA accumulation are not well understood. This study examined the effects of calcium chloride (CaCl2), phenobarbital (PB), methyl jasmonate (MeJA), and 5-azacytidine (5-AC) as exogenous inducers. The results showed GA contents of 26.35 ± 0.67, 31.53 ± 0.30, 24.66 ± 0.82, and 26.61 ± 0.37 mg/g dry weight (DW), respectively. Using Box–Behnken design (BBD) based on the response surface methodology (RSM), the optimal combination of inducers was determined to be 400.00 μM CaCl2, 1,087.96 μM PB, 260.67 μM MeJA, and 823.04 μM 5-AC (the Mix treatment), which led to a GA content of 31.94 mg/g DW, a 47.67% increase compared with the untreated control. Under 125 μM MeJA and the Mix treatment, the contents of GA-B and GA-D increased by 121.50- and 68.94-fold, respectively. Transcriptomic analysis revealed 233, 216, 1858, 488, and 699 differentially expressed genes (DEGs) in the five treatment groups. Among them, 5, 5, 2, 6, and 5 upregulated mevalonic acid (MVA) pathway genes and 4, 7, 69, 10, and 36 Cytochrome P450 (CYP) DEGs were identified, including a common downregulated CYP gene, GLB03G000748, suggesting its role in negative regulation of GA biosynthesis. This study not only demonstrates an effective combined induction strategy for the enhanced production of GAs by G. lucidum but also, through transcriptomic analysis, provides crucial molecular insights into the regulatory mechanisms of GA biosynthesis.","PeriodicalId":310067,"journal":{"name":"Medicinal Plant Biology","volume":"5 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147919256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection and functional identification of berberine biosynthetic genes in Berberis amurensis 小檗碱生物合成基因的选择与功能鉴定

Medicinal Plant Biology Pub Date : 2025-01-01 DOI: 10.48130/mpb-0025-0022

Syed Basit Ali Shah, Lingzhe Kong, Yuzhuo Dong, Yaning Fu, Ya Tian, Yupeng Du, Zhichao Xu, Ge Bai, Zhoujie An

{"title":"Selection and functional identification of berberine biosynthetic genes in Berberis amurensis","authors":"Syed Basit Ali Shah, Lingzhe Kong, Yuzhuo Dong, Yaning Fu, Ya Tian, Yupeng Du, Zhichao Xu, Ge Bai, Zhoujie An","doi":"10.48130/mpb-0025-0022","DOIUrl":"https://doi.org/10.48130/mpb-0025-0022","url":null,"abstract":"Berberine, a pharmacologically important benzylisoquinoline alkaloid (BIA) exhibiting antimicrobial and anti-inflammatory properties, accumulates abundantly in <italic>Berberis amurensis</italic>. However, there is a lack of reports concerning the berberine biosynthesis pathway in <italic>B. amurensis</italic>. In this study, berberine biosynthetic genes were systematically identified in <italic>B. amurensis, </italic>and key functional enzymes (BaNCS4, Ba6OMT4, Ba4'OMT2, and BaSOMT1) were characterized through transcriptomic and biochemical analyses. Notably, Ba4'OMT2 was found to exhibit superior catalytic efficiency compared to its ortholog Cc4'OMT, highlighting its potential as a valuable molecular tool for heterologous biosynthesis platforms. These findings not only elucidate the key enzymes of BIA skeleton formation and berberine <italic>O</italic>-methylation modification, as well as their evolutionary conservation but also offer potential molecular elements for achieving the biosynthesis of berberine through synthetic biology approaches.","PeriodicalId":310067,"journal":{"name":"Medicinal Plant Biology","volume":"4 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.maxapress.com/data/article/opr/preview/pdf/mpb-0025-0022.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A method of genetic transformation of Xizang medical plants without tissue culture 西藏药用植物不经组织培养的遗传转化方法

Medicinal Plant Biology Pub Date : 2025-01-01 DOI: 10.48130/mpb-0024-0032

Ting Sun, Xinghao Han, Yuanyuan Jiang, Qiulin Li, Yuanjiang Xu, Fangyuan Zhang, Xiaozhong Lan

引用次数: 2

Characterization of three flavonol synthases involved in the biosynthesis of 4'-deoxyflavonol in Scutellaria baicalensis Georgi 黄芩中参与4′-脱氧黄酮醇生物合成的三种黄酮醇合成酶的表征

Medicinal Plant Biology Pub Date : 2025-01-01 DOI: 10.48130/mpb-0025-0033

Sanming Zhu, Qiuying Chen, Yu Kong, Lihang Xie, Qing Zhao, Mengying Cui

引用次数: 0

Quasi-targeted metabolomics analysis reveals linkage between chemical composition and different post-harvest processing methods of Polygala tenuifolia 准靶向代谢组学分析揭示了黄芪化学成分与不同采后加工方法之间的联系。

Medicinal Plant Biology Pub Date : 2025-01-01 DOI: 10.48130/mpb-0025-0032

Shuai Liu, Jing Liu, Jingjing Li, Zongren Xu, Kun Lei, Jingjing Zhang, Yamei Gao, Zongsuo Liang, Yang Liu, Hong Zhang

引用次数: 0

Targeted metabolomics analysis of three medicinal plants of the genus Pulsatilla 白杨花属3种药用植物&lt；i&gt；

Medicinal Plant Biology Pub Date : 2024-01-01 DOI: 10.48130/mpb-0024-0025

Yanchang Huang, Che Bian, Yutong Huang, Wenjuan Hou, Hefei Xue, Yanping Xing, Han Zheng, Y. Y. Yang, Ting‐Guo Kang, Liang Xu

{"title":"Targeted metabolomics analysis of three medicinal plants of the genus Pulsatilla","authors":"Yanchang Huang, Che Bian, Yutong Huang, Wenjuan Hou, Hefei Xue, Yanping Xing, Han Zheng, Y. Y. Yang, Ting‐Guo Kang, Liang Xu","doi":"10.48130/mpb-0024-0025","DOIUrl":"https://doi.org/10.48130/mpb-0024-0025","url":null,"abstract":"Pulsatilla chinensis, a member of the Ranunculaceae family, is one of the 12 Pulsatilla species distributed in China. During the fruit-ripening period, the achenes of these plants tend to cluster densely in a head-like structure, and the style persists, resembling a silver filament. Resembling an old man with white hair, it is thus named Baitouweng. In this study, P. chinensis, Pulsatilla cernua (Thunb.) Bercht. & J. Presl, and Pulsatilla chinensis var. kissii (Mandl) S. H. Li et Y. H. Huan were selected as objects for a detailed targeted metabolomics analysis to determine whether P. chinensis var. kissii can serve as a substitute for P. chinensis-based medicinal materials. A total of 1,558 compounds were identified. Among these, 392 were primary metabolites, 1,166 were secondary metabolites, and 47 were triterpenoid saponins. Clustering analysis of the total metabolites revealed that the three Pulsatilla species clustered based on their population origin, rather than their variety. The differential metabolite analysis of triterpenoid saponins showed that metabolic differences between P. chinensis var. kissii and P. chinensis were more pronounced than those between P. chinensis var. kissii and P. cernua. In conclusion, P. chinensis var. kissii is more suitable as a substitute for P. cernua medicinal materials. This result lays a foundation for in-depth investigations into the potential internal relationships among these three Pulsatilla species.","PeriodicalId":310067,"journal":{"name":"Medicinal Plant Biology","volume":"3 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.maxapress.com/data/article/opr/preview/pdf/mpb-0024-0025.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147903403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Characterization of two pterocarpan glycosyltransferases in Astragalus membranaceus and their application in whole-cell biocatalysis 黄芪中两种翼龙糖基转移酶的表征及其在全细胞生物催化中的应用

Medicinal Plant Biology Pub Date : 2024-01-01 DOI: 10.48130/mpb-0024-0013

Bai‐Han Gao, Meng Zhang, Kuan Chen, Linlin Wang, Mingju Yao, Hongye Li, Ming Xiong, Yuxi Huang, Yang Zhang, Quanli Liu, Juan Guo, Min Ye, Xue Qiao

{"title":"Characterization of two pterocarpan glycosyltransferases in Astragalus membranaceus and their application in whole-cell biocatalysis","authors":"Bai‐Han Gao, Meng Zhang, Kuan Chen, Linlin Wang, Mingju Yao, Hongye Li, Ming Xiong, Yuxi Huang, Yang Zhang, Quanli Liu, Juan Guo, Min Ye, Xue Qiao","doi":"10.48130/mpb-0024-0013","DOIUrl":"https://doi.org/10.48130/mpb-0024-0013","url":null,"abstract":"Pterocarpan glucosides are biologically active natural products, in which the glucosyl group plays an important role. However, specific pterocarpan glycosyltransferases have rarely been reported. In this study, we identified two highly efficient glycosyltransferases, AmGT28 and AmGT44, from <italic>Astragalus membranaceus</italic>, catalyzing the conversion of medicarpin (<bold>1</bold>) to medicarpin 3-<italic>O</italic>-glucoside (<bold>1a</bold>). They could also use other pterocarpans and isoflavonoids as substrates, showing a preference for pterocarpans over isoflavonoids. A new isoflavone 7-<italic>O</italic>-glucoside <bold>5a</bold> was prepared through scaled-up enzymatic reaction. Furthermore, AmGT44 was expressed in <italic>E. coli</italic> to establish a whole-cell biocatalytic system (500 μL with 0.1 mM substrate), which could glucosylate pterocarpans <bold>1</bold>-<bold>3</bold> with a conversion rate of up to 100% and a titer of 30-38 μg/mL within 25 hours. The titer of maackiain 3-<italic>O</italic>-glucoside (<bold>2a</bold>) could reach 78.66 μg/mL in a 10-mL system. The study provides efficient catalytic tools for the biosynthesis of pterocarpan glycosides.","PeriodicalId":310067,"journal":{"name":"Medicinal Plant Biology","volume":"3 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Discovery of anti-SARS-CoV-2 agents from commercially available flavor via docking screening 通过对接筛选，从市售香精中发现抗sars - cov -2药物

Medicinal Plant Biology Pub Date : 2023-01-01 DOI: 10.48130/mpb-2023-0010

Jiaojiao Zhang, Xin Shen, Yanpeng Li, Yong‐Ming Yan, Yan Wang, Yong-Xian Cheng

引用次数: 2

Discovery of anti-SARS-CoV-2 agents from commercially available flavor via docking screening 通过对接筛选从市售香精中发现抗sars - cov -2药物

Medicinal Plant Biology Pub Date : 2020-03-02 DOI: 10.31219/osf.io/vjch2

Jiao-Jiao Zhang, Xin Shen, Yong-Ming Yan, Yan Wang, Yong‐Xian Cheng

引用次数: 19

Diterpenoids from Scutellaria barbata induce tumour-selective cytotoxicity by taking the brakes off apoptosis 黄芩中的二萜类化合物通过抑制细胞凋亡来诱导肿瘤选择性细胞毒性

Medicinal Plant Biology Pub Date : 1900-01-01 DOI: 10.48130/mpb-2022-0003

Matthew L. Tomlinson, Man Zhao, Elaine J. Barclay, Jie Li, Haixiu Li, J. Felix, L. Hill, G. Saalbach, M. Rejžek, Dongfeng Yang, Qing Zhao, Paul Kroon, Wei Wang, Y. Bao, M. Howes, E. Tatsis, Cathie Martin

引用次数: 3