Tissue culture research communications : the journal of experimental & applied cell culture research最新文献_第7页

PLATELET-LIKE PARTICLES RELEASED BY INHIBITION OF DNA SYNTHESIS IN THE HUMAN-MEGAKARYOBLASTIC LEUKEMIA CELL LINE, MEG-01s 抑制人巨核母细胞白血病细胞系DNA合成释放的血小板样颗粒

Tissue culture research communications : the journal of experimental & applied cell culture research Pub Date : 1995-09-30 DOI: 10.11418/JTCA1981.14.3_165

M. Takeuchi, H. Kuno, M. Satoh, Touho Yoshida, M. Ogura, Kikuko Takeuchi

{"title":"PLATELET-LIKE PARTICLES RELEASED BY INHIBITION OF DNA SYNTHESIS IN THE HUMAN-MEGAKARYOBLASTIC LEUKEMIA CELL LINE, MEG-01s","authors":"M. Takeuchi, H. Kuno, M. Satoh, Touho Yoshida, M. Ogura, Kikuko Takeuchi","doi":"10.11418/JTCA1981.14.3_165","DOIUrl":"https://doi.org/10.11418/JTCA1981.14.3_165","url":null,"abstract":"The human megakaryoblastic cell line, MEG-01s, released particles identified by a characteristic marginal microtubule bundle and by the localization of platelet-specific glycoprotein (GPIlb/Illa) in the plasma membrane, detected by an immunofluorescence method. The fluorescence image and size of these particles were similar to these features in human blood platelets. To analyze the regulation of particle release by MEG-01s, the effects of DNA synthesis inhibitors, tumor promoters, and protein kinase inhibitors on these cells were examined by an immunofluorescence method. The release of particles from MEG-Ols cells was enhanced by inhibitors of DNA synthesis : aphidicolin, 5-bromodeoxyuridine, 5-fluorodeoxyuridine, hydroxyurea, etoposide, and camptothecin. The particle release was specific to the MEG-01s cells, and did not occur in the myeloid leukemic cells, HL-60. These results suggest that the cessation of DNA synthesis may trigger terminal differentiation by synthesis of factors involving particle release by MEG-01s.","PeriodicalId":308966,"journal":{"name":"Tissue culture research communications : the journal of experimental & applied cell culture research","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130498961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

DIFFERENTIATION OF AVIAN DIGESTIVE TRACT EPITHELIUM IN VITRO 禽消化道上皮的体外分化

Tissue culture research communications : the journal of experimental & applied cell culture research Pub Date : 1995-09-30 DOI: 10.11418/JTCA1981.14.3_177

S. Yasugi

{"title":"DIFFERENTIATION OF AVIAN DIGESTIVE TRACT EPITHELIUM IN VITRO","authors":"S. Yasugi","doi":"10.11418/JTCA1981.14.3_177","DOIUrl":"https://doi.org/10.11418/JTCA1981.14.3_177","url":null,"abstract":"Avian embryonic digestive tract has been used for the analysis of epithelialmesenchymal interactions in the organogenesis. Many tissue recombination experiments carried out so far demonstrated that the morphological differentiation of the epithelium is often determined by the influences of the mesenchyme, whereas the functional, molecular differentiation depends on both the mesenchymal influence and the reactivity of the epithelium. The epithelium of the avian embryonic digestive tract can be classified into two parts : cells in one part can express pepsinogen gene, a marker gene of the proventriculus (glandular stomach) under certain conditions and cells in another part never express it. The expression of ECPg gene in epithelial cells is controlled by the action of the mesenchyme, through 5' promoter region of ECPg gene. The establishment of two quantitatively different parts in digestive tract epithelium occurs just after the formation of endoderm, and is considered to be accompanied by the expression of some homeobox genes. The elucidation of functions of these genes may greatly contribute to the understanding of the mechanisms underlying organogenesis of many organ systems.","PeriodicalId":308966,"journal":{"name":"Tissue culture research communications : the journal of experimental & applied cell culture research","volume":"213 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1995-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117218769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

INTERACTION OF ENDOTHELIAL CELLS AND MYOCYTES IN CARDIOVASCULAR SYSTEM 心血管系统中内皮细胞与肌细胞的相互作用

Tissue culture research communications : the journal of experimental & applied cell culture research Pub Date : 1995-09-30 DOI: 10.11418/JTCA1981.14.3_185

T. Kumazaki, Takahiko Suzuki, Y. Mitsui

引用次数: 0

DNA FRAGMENTATION IN APOPTOSIS 细胞凋亡中的DNA断裂

Tissue culture research communications : the journal of experimental & applied cell culture research Pub Date : 1995-06-30 DOI: 10.11418/JTCA1981.14.2_133

Tanuma Sei-ichi

引用次数: 1

IMPROVED TRANSFECTION EFFICIENCY OF 3Y1 AND ITS DERIVATIVE CELLS 提高3y1及其衍生细胞的转染效率

Tissue culture research communications : the journal of experimental & applied cell culture research Pub Date : 1995-06-30 DOI: 10.11418/JTCA1981.14.2_141

Y. Hatakeyama, N. Tsuyama, Takayo Sasaki, T. Ide

引用次数: 1

HUMAN CELL LINE IDENTIFICATION BASED ON D1S80 AND HLA DQα GENOTYPING BY PCR-AMPLIFICATION OF ALLELES 基于d1680和hla - dqα基因型的人细胞系鉴定

Tissue culture research communications : the journal of experimental & applied cell culture research Pub Date : 1995-06-30 DOI: 10.11418/JTCA1981.14.2_103

Masanori Takahashi, Yukie Kato, Manaka Ken-ichi, H. Mukoyama, George Miyakawa, S. Kamiyama

引用次数: 0

OSTEOBLASTIC CHARACTER OF FIBROBLASTS DERIVED FROM HUMAN PERIODONTAL LIGAMENT 人牙周韧带成纤维细胞成骨特性的研究

Tissue culture research communications : the journal of experimental & applied cell culture research Pub Date : 1995-06-30 DOI: 10.11418/JTCA1981.14.2_109

T. Kawase, S. Saito

引用次数: 0

REGULATION OF APOPTOSIS IN VIRUS-INFECTED CELLS 病毒感染细胞凋亡的调控

Tissue culture research communications : the journal of experimental & applied cell culture research Pub Date : 1995-06-30 DOI: 10.11418/JTCA1981.14.2_127

Y. Nakanishi

引用次数: 2