Journal of Ardabil University of Medical Sciences最新文献

{"title":"The Effect of Four Weeks of Aerobic Training on the Expression of Sirt1, CREB and BDNF Genes in Hippocampus of Male Wistar Rats with Alzheimer’s Disease","authors":"Tahereh Dalir, R. Gharakhanlou, M. Peeri, Hasan Matin Homaee","doi":"10.52547/jarums.20.4.562","DOIUrl":"https://doi.org/10.52547/jarums.20.4.562","url":null,"abstract":"Background & objectives: Exercise, with beneficial effects on brain health and cognitive function reduces the destructive effects of some neurological diseases such as Alzheimer's. The aim of this study was to evaluate the effect of four weeks of aerobic exercise on cognitive function and expression of Sirt1, CREB and BDNF genes in the hippocampus of male Wistar rats with Alzheimer's disease. Methods: The statistical population included 18 male Wistar rats from the Pasteur Institute. Rats were randomly divided into three groups including Alzheimer's group, Alzheimer's disease-exercise group and a healthy control group. Alzheimer's disease group was induced by injecting Aβ42 into the hippocampus. Seven days after surgery, the rats performed the aerobic exercise for four weeks (five sessions per week at a speed of 10-15 m/min). They underwent behavioral tests 48 hours after the last training session. Twenty four hours later, rat hippocampal tissue was extracted. Sirt1, CREB and BDNF mRNAs were measured using Real time-PCR. Results: Learning and spatial memory performance decreased in rats of Alzheimer's disease group compared to a healthy control group (p˂0.001). Decreased mRNA expression of Sirt1, CREB and BDNF genes was observed in the hippocampal tissue of Alzheimer's disease group compared with the healthy control group (p˂0.001). Alzheimer's rats with intermittent aerobic exercise had improved learning function, spatial memory and increased mRNA expression levels of Sirt1, CREB and BDNF genes in comparison with Alzheimer's disease group (p˂0.001). Conclusions: Periodic aerobic exercise in rats with Alzheimer's disease can improve spatial learning and memory by positively regulating the Sirt1/ CREB/ BDNF signaling pathway in hippocampal tissue.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43635558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Alpha-pinene on Amyloid-beta-induced Neuronal Death and Depression in Male Wistar Rats","authors":"Mohammad-Kazem Khan-Mohammadi-Khorrami, M. Asle-Rousta, M. Rahnema, Rahim Amini","doi":"10.52547/jarums.20.4.456","DOIUrl":"https://doi.org/10.52547/jarums.20.4.456","url":null,"abstract":"Background & objectives: The deposition of amyloid beta (Aβ) peptide in the brain is one of the most important features of Alzheimer's disease. In addition to memory loss, Aβ can lead to depression behavior. Alpha-pinene is a type of monoterpene that has antioxidant, antiinflammatory, and neuroprotective effects. Here, by using an animal model for Alzheimer's disease, we investigated the effect of alpha-pinene on neuronal cell death in the hippocampus and depression induced by Aβ1-42. Methods: Male Wistar rats weighing 240-260 g were divided into four groups including control, alpha-pinene, Aβ, and Aβ-alpha-pinene. Rats were placed in stereotaxic surgery apparatus and Aβ1-42 was injected into the hippocampus (4 μg per side) and alpha-pinene was treated intraperitoneally (50 mg/kg) for 14 consecutive days. At the end of the course, the level of depression was assessed using the forced swimming test. The animals' hippocampus was also examined microscopically after Nissl staining. Results: Intra-hippocampal injection of Aβ1-42 increased the total immobility time in the forced swimming test (p<0.01), decreased the number of pyramidal neurons in the CA1 area (p<0.001), and reduced the thickness of the neuronal layer in this region of the hippocampus. Treatment with alpha-pinene largely prevented these changes. Conclusion: It can be concluded that alpha-pinene decreased the beta-amyloid-induced depressive behavior in rats and inhibited the neuronal loss, suggesting that this neuroprotective compound may have a critical role in depression. Alpha-pinene is probably a suitable therapeutic strategy for repressing Aβ-induced neurodegeneration.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44385094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship between Body Mass Index and Lumbar Bone Mineral Density in Active and Inactive Middle-aged Women","authors":"B. Tartibian, Leila Fasihi, R. Eslami","doi":"10.52547/jarums.20.4.465","DOIUrl":"https://doi.org/10.52547/jarums.20.4.465","url":null,"abstract":"Background & objectives: Obesity and osteoporosis are major problem that their prevalence is increasing. Physical activity can be effective in the prevention of osteoporosis by some mechanisms such as changes in anthropometrics variables. Body mass index (BMI) in men and women is closely related to bone mineral density (BMD). However, the relationship varies between BMI and BMD according to the different studies. Therefore, the aim of this study was to investigate the relationship between body mass index and lumbar bone mineral density in active and inactive middle-aged women. Methods: Sixty active, middle-aged women and 60 inactive women in the age range of 50 to 65 years, with medical records and clinical trials were selected in Milad Hospital in Tehran. Body mass index was calculated and bone mineral density was measured by DEXA scan in the lumbar spine (L1 to L4). Independent t-test and Pearson correlation coefficient were used to evaluate the data. SPSS software version 26 was used for data analysis. Results: The results of this study showed that in the active group in L1, L3 and L4 lumbar vertebrae (p=0.034, p=0.017, p=0.019, respectively), and in the inactive group in L3 and L4 vertebrae (p=0.034, p=0.022, respectively), there was a positive and significant relationship between body mass index and bone mineral density. No significant relationship was found in other lumbar vertebrae of both groups. Conclusion: The results of the present study showed that weight gain and consequently BMI among active women reduce the risk of osteoporosis. Physical activity in women seems to lead to more muscle mass, which in turn leads to an increase in bone mineral density. Considering this issue, it can be said that one of the applications of the results of the present study is the use of this index in predicting the bone density of individuals.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43572632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of a Balanced Low-Calorie Diet and Slight Weight Loss on Body Image Score and Its Subscales","authors":"F. Ghannadiasl, M. Mohajeri, N. Hoseini","doi":"10.52547/jarums.20.4.495","DOIUrl":"https://doi.org/10.52547/jarums.20.4.495","url":null,"abstract":"Background & objectives: Considering the high prevalence of body image dissatisfaction and its consequences, it is necessary to consider effective interventions to reduce it. The aim of this study was to investigate the effect of a balanced low calorie diet (BLCD) and slight weight loss on the body image score and its subscales. Methods: In this clinical trial, 74 apparently healthy, non-pregnant, non-lactating and nonmenopausal women (age range: 18-49 years with body mass index range: 27.5-40 kg/m) were selected using random sampling method from a nutrition clinic in their first visit in Ardabil city between November 2019 and February 2020. Data collection was done by Anthropometric measurements and multidimensional body self-relations questionnaire. The paired T-test was used to compare the changes during 4 week balanced low calorie diet intervention. BLCD was an individualized diet with an energy deficit of 500 calories of daily energy intake that was calculated from 3-day food records for every person. Results: After 4 weeks, BLCD resulted in a significant decrease in body weight (p=0.001), body mass index (p=0.001), waist circumference (p=0.002), waist to height ratio (p=0.012), abdominal volume index (p=0.009) and conicity index (p=0.045) and a significant increase in the subscale scores of fitness evaluation (p=0.01), health orientation (p=0.03), body area satisfaction (p=0.01) and overweight preoccupation (p=0.001). Conclusion: According to the results, it seems that even slight weight loss with a balanced low-calorie diet can be effective in improving anthropometric indices and some subscales of body image in 4 weeks. These results support the effectiveness of short-term interventions.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47603591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Resveratrol on Vincristine-induced Oxidative Stress in Mouse Ovary and UterineTissue","authors":"M. Nasirzadeh, S. Hejazi, Mohammad Bakhshi, Saeed Taginasab, Heidar Tayefesattari, Pouya Navidi far","doi":"10.52547/jarums.20.4.474","DOIUrl":"https://doi.org/10.52547/jarums.20.4.474","url":null,"abstract":"Background & objectives: Vincristine (VIN) is a broad-spectrum anticancer drug has been used to treat various cancers. Resveratrol (Res) is a natural polyphenol found in many plant sources. Many studies have reported anti-inflammatory and antioxidative effects of resveratrol. We have explored the protective effect of resveratrol on vincristine-induced oxidative stress in mouse ovarian tissue. Methods: In this study, 32 female mice weighing 25-30 grams were randomly divided into four groups (each group n=8): 1control group, 2Vingroup, 3Vin-Res group and 4Res group. The mice received a single IP injection of vincristine (3 mg/kg) weekly for 4 weeks. Res treatment was done 28 days by gastric gavage (daily 30 mg/kg).At the end of the study, Malondialdehyde (MDA) and total antioxidant capacity (TAC) and the antioxidant activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzymes were measured in ovarian tissue and uterus of the studied animals. Also, ovarian follicles were counted. Results: The results indicated that the MDA level was elevated and TAC, GPx as well as SOD activities were decreased in Vingroup significantly. Resveratrol reduced MDA level and increased GPx and SOD activities in Vin-Res group significantly. Also histological findings showed that Res increased primordial and primary follicles and reduced atretic follicles in Vin-Res group significantly. Conclusions: These results indicate the protective effect of resveratrol on ovarian and uterine tissue against oxidative damage of vincristine in mice.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44050549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Eight Weeks of Resistance Training-Cold Water Immersion and Curcumin Supplementation on Muscle Injury and Inflammatory Response Following Acute Strength Activity in Futsal players Men","authors":"A. Ahmadzadeh, A. Pourrahim Ghouroghchi, R. Afroundeh","doi":"10.52547/jarums.20.4.533","DOIUrl":"https://doi.org/10.52547/jarums.20.4.533","url":null,"abstract":"Background & objectives: Minimizing muscle damage and inflammation can boost the immune system and improve sport performance. The aim of this study was to investigate the effect of 8 weeks of resistance training-cold water immersion and curcumin supplementation on muscle injury and inflammation following a session of acute strength activity in futsal players men. Methods: twenty futsalist volunteered to participate in the study and were randomly assigned to experimental and control groups. Blood sampling was performed after 12 hours of night fasting during four stages. After the first phase of blood sampling, both groups were performed one session of acute circular strength training, then, the second phase blood sampling was performed. 48 hours later, the experimental group performed 8 weeks of circular resistance training, three sessions per week as in the acute strength training and were immersed immediately after exercise, 20minutes at 5-10°C in cold water and used 150mg curcumin supplementation. Forty eight hours after the last training session and last using curcumin, blood sampling was performed in both groups. Then, both experimental and control groups performed the second acute circular strength training and the fourth stage blood sampling was performed. The differences of variables at different time intervals were compared with 2×4 two-way ANOVA and differences between the two groups were investigated with Independent-Samples T-Test at significance level of p<0.05. Results: Creatine kinase and white blood cells significantly increased in both groups after the first acute strength activity (p=0.049). Performing 8 weeks of resistance training-cold water immersion and curcumin supplementation, significantly decreased creatine kinase and white blood cell counts (p=0.024). Also, 8 weeks of resistance training-cold water immersion and curcumin supplementation, significantly reduced creatine kinase and white blood cells following acute second strength activity (p=0.021). Conclusion: Appears to, 8-week resistance training-cold water immersion and curcumin supplementation following a session of acute strength training reduce muscle injury and inflammation in futsal players men.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49500730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Evaluation of Honey Throat Spray for Auxiliary Treatment of Pharyngitis","authors":"Davar Nikzadeh Kigal, S. Habibzadeh, H. Ebrahimi, Masoumeh Mohammadi Monfared, Mansour Miran","doi":"10.29252/jarums.20.1.79","DOIUrl":"https://doi.org/10.29252/jarums.20.1.79","url":null,"abstract":"Background & objectives: Due to the increasing prevalence of bacterial resistance, there is a growing attention to application of auxiliary treatment of pharyngitis. The aim of this study was to investigate the effect of honey throat spray in the auxiliary treatment of patients with a diagnosis of pharyngitis. Methods: At the start, different physicochemical quality parameters of the honey sample were evaluated. Then, the bottles of honey and the placebo throat spray were prepared. The numbers of 70 patients with the diagnosis of pharyngitis were included in a one-step and double-blinded study. In addition to standard treatment, each patient received either one of the honey or placebo throat spray. The clinical symptoms of the patients were compared between the two groups at baseline and then at 24 hours, 48 hours, 5 days and 10 days after the starting the treatment procedure. Results: As per results, the quality of honey sample was met to the standard honey criteria. The treatment and placebo groups were statistically similar in terms of demographic specifications or clinical symptoms at the time to enter the study. Pharyngitis recovery was better in treatment group than in the placebo group; this positive effect was statistically significant for symptoms of morning phlegm and painful swallowing, 24 hours after the starting treatment. In treatment group, after 48 hours, symptoms of fever and painful swallowing were resolved, while this indicator was 23% in placebo group. Also, throat pain of 85.71% of patients in treatment group and 17.14% of patients in placebo group was relieved. Overall, the mean recovery time in treatment group was shorter than placebo group. Conclusion: The findings of this study showed that the use of honey throat spray as auxiliary treatment of pharyngitis can improve the clinical symptoms of pharyngitis and reduce recovery time.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":"20 1","pages":"79-93"},"PeriodicalIF":0.0,"publicationDate":"2020-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44540111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of 12 Weeks of Pilates Training and Ginger Consumption on Serum Levels of BDNF and TNF-α in Women with Multiple Sclerosis","authors":"S. Babaei Bonab","doi":"10.52547/jarums.20.3.307","DOIUrl":"https://doi.org/10.52547/jarums.20.3.307","url":null,"abstract":"","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41586754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Effect of Endurance Training and Supplementation of Persia Rosa Extract on Mitochondrial Apoptosis of Myocardium","authors":"A. Imani, M. Siahkouhian, Pouran Karimi, Masoud Asgharpour-Arshad, F. Seifi-Skishahr","doi":"10.29252/jarums.20.1.59","DOIUrl":"https://doi.org/10.29252/jarums.20.1.59","url":null,"abstract":"Background & objectives: Physical training and some supplements through different mechanisms could be effective in reducing apoptosis; the aim of this study was to investigate the effect of endurance training and supplementation of Persia Rosa extract on mitochondrial apoptosis of myocardium. Methods: In this experimental study, 52 male rats were randomly assigned into five homogeneous groups, three-month control, six months control, Persia Rosa supplement, endurance training and endurance training+Persia Rosa. Training groups participated in endurance training protocol for 12 weeks. The Persia Rosa and endurance training+Persia Rosa groups received 1 gram Persia Rosa extract per kg body weight. After the implementation of the training protocol and consumption of the extract, the surgical and cardiac tissue extraction procedures were performed. The data were analyzed by ANOVA test and Bonferroni post hoc tests at the significant level of less than 5% using SPSS-22 software. Results: The results showed that Bax/Bcl-2 ratio and Caspase-3 gene expression were significantly decreased in endurance training and endurance training+Persia Rosa groups to the control group (p˂0.05). Bax/Bcl-2 showed a significant decrease in the Persia Rosa supplement group (p˂0.05), but Caspase-3 was not significant in the Persia Rosa supplement group. Conclusion: It is expected that the effect of endurance training with consumption of Persia Rosa extract on Bax/Bcl-2 ratio and Caspase-3 will provide a good platform for caspase mechanisms and apoptosis processes in the myocardium of rats.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":"20 1","pages":"59-78"},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48615771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of Potential Anti-Leishmanial Pharmacophores via In Silico Drug Repurposing","authors":"Sana Ahdeno, Nima Razaghi-Asl, B. Mohammadi-ghalehbin","doi":"10.52547/jarums.20.3.372","DOIUrl":"https://doi.org/10.52547/jarums.20.3.372","url":null,"abstract":"Background & objectives: Drug-repurposing is the study on application of existing drugs for treatment or control of other diseases. Major advantage of the technique relies on a nominated drug molecule that is applied for pharmacodynamics optimization due to lack of serious pharmacokinetics challenges. According to the importance of the subject, a present contribution has been dedicated to the in-silico analysis of a few drug classes with the aim of achieving potential anti-leishmanial pharmacophores. Methods: 3D structure of protein targets within leishmania parasite were retrieved from Brookhaven Protein Data Bank (PDB) on the basis of literature reports to evaluate the related complexes with drugs via molecular docking. Qualitative and quantitative analysis of drugtarget interaction patterns in docked complexes offered drugs with higher binding affinities toward targets and finally structural patterns or hypothetical anti-leishmanial pharmacophores were proposed with regard to the top-ranked pharmaceutical compounds. Results: Highest free binding energy could be estimated for Nateglinide in binding to farnesyl diphosphate synthase (ΔGb -13.30 kcal/mol). Among steroids, Norgestrel synthase (ΔGb -9.48 kcal/mol) and Testosterone synthase (ΔGb -8.05 kcal/mol) exhibited higher enzyme binding affinities and Arg82 was a key residue in making hydrogen bonds. Within fused tricyclic structural patterns, mirtazapine exhibited highest binding affinity to deoxy uridine triphosphate (ΔGb -8.64 kcal/mol). In Carbamazepine, amide substituent of the central ring facilitated the formation of two effective hydrogen bonds with Gln21 and Asn25 in deoxy uridine triphosphate. Conclusion: On the basis of obtained results for steroids and fused tricyclic scaffolds, it will be possible to design molecules that can inhibit several pathogenic targets simultaneously.","PeriodicalId":30758,"journal":{"name":"Journal of Ardabil University of Medical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48278504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}