L. Oliveira, Jacqueline G. S. Haimuri, C. Jacyntho, Tomas Z Barrese, G. Vasconcelos, S. Cavalcanti, M. Rosa
{"title":"HUMAN PAPILLOMAVIRUS INFECTION IN HUMAN IMMUNODEFICIENCY VIRUS POSITIVE WOMEN UNDER ROUTINE PAP SMEAR","authors":"L. Oliveira, Jacqueline G. S. Haimuri, C. Jacyntho, Tomas Z Barrese, G. Vasconcelos, S. Cavalcanti, M. Rosa","doi":"10.17525/VRRJOURNAL.V13I1-2.15","DOIUrl":"https://doi.org/10.17525/VRRJOURNAL.V13I1-2.15","url":null,"abstract":"The present study aimed to investigate HPV prevalence associated with cervical cytology and to evaluate data from 140 HIV positive women routinely attended at an out-patient public gynecological service in Rio de Janeiro state. The CD4 cell counts and HIV-1 RNA levels were determined using standardized protocols. HPV status and HPV 16 detection were ascertained respectively by My09/11 consensus primers and type specific primers. Colpocytology was performed at the first or subsequent visit to the service. Among women, 98.6% of them were under antiviral treatment, 92.1% had over 200 CD4 cells/mm3, 62.9% had detectable HIV RNA, 60.7% were HPV infected and 21.2 % harbored the HPV type 16. Normal/inflammatory results were prevalent (57.1%) and 7.9% of the patients had HSIL. Studying demographic data and HIV markers that could affect HPV infection, we found that time elapsed since positive diagnosis of less than four years was a significant factor. First sexual intercourse beginning earlier than 17 years, people younger than 30 years and white ethnicity were also linked to HPV infection. Alcohol use was marginally related to HPV prevalence. Patients with severe immune depression were strongly predisposed to HPV infection (from 90.9% of patients carrying less than 200 cells/mm3 to 45.5% in those with CD4 cell level above 500 cells/mm3) and the development of cervical lesions. There was no relation between HIV immune status and HPV type 16, but HIV seropositive women with abnormal cytology were three times more likely to harbor this HPV type. The high HPV prevalence as opposed to absence of cancer cases was probably due to the moderate immune status of the women studied added to routine cancer prevention exams. DOI: http://dx.doi.org/10.17525/vrrjournal.v13i1-2.15","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"13 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2008-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DETECTION OF HUMAN METAPNEUMOVIRUS IN NASOPHARYNGEAL ASPIRATES OF BRAZILIAN CHILDREN BY IMMUNOFLUORESCENCE ASSAY","authors":"J. F. Ribeiro, F. Moura","doi":"10.17525/VRR.V13I1-2.17","DOIUrl":"https://doi.org/10.17525/VRR.V13I1-2.17","url":null,"abstract":"The circulation of human metapneumovirus is related to seasonal patterns of respiratory syncytial virus. The aim of this study was to determine the frequency of hMPV infection diagnosed by indirect immunofluorescence in children attending emergency department and general wards of Hospital Infantil Albert Sabin in Fortaleza, Ceara, northeastern Brazil, during the respiratory syncytial virus season in 2006. The analysis included 389 samples. Antigens of hPMV were detected in 39 samples. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.17","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"25 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2008-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Gomes, Débora M. Cerqueira, D. Falcão, F. S. Menezes, M. Wigg, G. Mendes, F. Martins, J.F.M. Silva, R. Kuster, M. Romanos
{"title":"IN VITRO ANTI-HSV-2 ACTIVITY OF ISOQUERCETIN FROM HYPTIS FASCICULATA BENTH.","authors":"M. Gomes, Débora M. Cerqueira, D. Falcão, F. S. Menezes, M. Wigg, G. Mendes, F. Martins, J.F.M. Silva, R. Kuster, M. Romanos","doi":"10.17525/VRR.V13I1-2.4","DOIUrl":"https://doi.org/10.17525/VRR.V13I1-2.4","url":null,"abstract":"","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2008-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Vieira, Glauciane da Silva Bifano, E. Honda, M. Tada, W. C. Batista
{"title":"ISOLATION AND IDENTIFICATION OF DENGUE VIRUS SEROTYPE 3 IN RONDONIA, BRAZIL","authors":"D. Vieira, Glauciane da Silva Bifano, E. Honda, M. Tada, W. C. Batista","doi":"10.17525/VRRJOURNAL.V13I1-2.16","DOIUrl":"https://doi.org/10.17525/VRRJOURNAL.V13I1-2.16","url":null,"abstract":"Dengue Fever is a serious public health problem in tropical and subtropical regions of the world. The state of Rondonia has 52 municipalities, of which 32 are found inside Amazonia Legal and 20 in border areas. In the period of January 2004 until May 2006, 160 blood samples were colleted from patients suspected of having DENV disease during outbreaks of dengue fever in the municipalities of Ariquemes, Jaru, Ouro Preto do Oeste, Cacoal, Colorado do Oeste, Vilhena and Porto Velho. Samples were submitted for viral isolation following RNA viral extraction and RT-PCR, in order to identify the dengue virus. Afterwards, hemi-nested-PCR was used to identify the viral serotype. Seventy eight samples were characterized as DENV-3. The amplicons characterized by hemi-nested-PCR were sequenced for similar research with nucleotide sequences of DENV-3 deposited in Genbank, using the BLAST program. DOI: http://dx.doi.org/10.17525/vrrjournal.v13i1-2.16","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"13 1","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2008-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Cavalcanti, L. Afonso, N. Moyses, I. M. Magalhães, M. Passos, L. Oliveira
{"title":"HUMAN PAPILLOMAVIRUS (HPV) INFECTION OF SEXUAL PARTNERS OF WOMEN PRESENTING CERVICAL INTRAEPITHELIAL NEOPLASIA","authors":"S. Cavalcanti, L. Afonso, N. Moyses, I. M. Magalhães, M. Passos, L. Oliveira","doi":"10.17525/VRR.V13I1-2.20","DOIUrl":"https://doi.org/10.17525/VRR.V13I1-2.20","url":null,"abstract":"Human papillomavirus (HPV) infections of the genital tract are the most prevalent sexually transmitted viruses worldwide. Oncogenic HPV types cause pre-malignant lesions that can progress to cervical carcinoma. In the male genital tract, most HPV infections are sub-clinical and associated with a vicious circle [stet] of treatment-reinfection of women. Nevertheless, HPV pathogenic processes are still poorly understood. The literature suggests that different HPV types can be found among sexual partners. In our study, we aimed to verify HPV infections in female patients as well as in their sexual partners, to test this hypothesis. The HPV DNA prevalence in women with Cervical Intraepithelial Neoplasia (CIN) was 92.5% compared with 25% for normal women, with a statistically significant difference (p<0.001). In male samples, the HPV DNA prevalence in partners from CIN women was 50% and for normal women partners, it was 17.5%. In the group of CIN women, we observed that 20 couples had HPV DNA in both partners. However, only 50% of the couples shared the same HPV type. In the group of normal women, only 6 couples were simultaneously infected by HPV, and from them only 33.3% had the same virus type. These results may be attributed to differences in local immunity and organization of the genital epithelia of each sex. On the other hand, female lesions would not be the result of re-infection by sexual partners, but rather a true recurrence of a latent infection. Finally, such 40% of agreement among all couples leads us to suggest a vicious circle [stet] of infectious processes, perpetuating HPV in the sexually active population. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.20","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"13 1","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2008-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. O. T. Carrasco, O. C. F. Neto, S. L. S. Lages, R. Sousa, A. B. Junior, A. A. Pinto
{"title":"ADAPTATION OF HEMAGGLUTINATION INHIBITION TECHNIQUE (HI) FOR THE DIAGNOSIS OF NEWCASTLE DISEASE IN OSTRICHES (Struthio camellus)","authors":"A. O. T. Carrasco, O. C. F. Neto, S. L. S. Lages, R. Sousa, A. B. Junior, A. A. Pinto","doi":"10.17525/VRR.V13I1-2.3","DOIUrl":"https://doi.org/10.17525/VRR.V13I1-2.3","url":null,"abstract":"The breeding of ratites (ostriches, emus and rheas) has expanded considerably all over the world in recent years. The hemagglutination inhibition (HI) test is considered to be the standard serology test, for detection of antibodies against Newcastle Disease Virus (NDV). However, HI test tends to give false positive results in serum samples of some species, including ostriches. In this study, kaolin was used to eliminate the nonspecific hemagglutination inhibitors in ostrich serum samples. Twenty-seven non-kaolin-treated ostrich samples reacted to the test and the titres ranged from 2 to 64. However, these samples did not react when they were kaolin-treated. The controls did not show any variance on antibodies titres, regardless of treatment with kaolin. The use of kaolin in the routine serodiagnosis of ND not only relieves adsorption of serum samples with avian erythrocytes, but also contributes to the reduction in the maintenance of red blood cells donor birds. DOI: http://dx.doi.org/10.17525/vrr.v13i1-2.3","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"12 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2008-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bruno R. Simonetti, D. C. Abreu, J. Simonetti, M. C. Gonçalves, M. Silva, O. Barth, H. Schatzmayr
{"title":"ANIMAL INFECTIONS BY VACCINIA-LIKE VIRUSES IN THE STATE OF RIO DE JANEIRO: 1- NORTHWESTERN REGION","authors":"Bruno R. Simonetti, D. C. Abreu, J. Simonetti, M. C. Gonçalves, M. Silva, O. Barth, H. Schatzmayr","doi":"10.17525/VRR.V12I1-2.10","DOIUrl":"https://doi.org/10.17525/VRR.V12I1-2.10","url":null,"abstract":"Orthopoxvirus infections in dairy cattle have been followed in the northwestern region of the state of Rio de Janeiro for the last nine years. Clinical specimens from 50 animals collected by Animal Health authorities have been received in our laboratory for laboratory diagnosis, most of them blood specimens. Orthopoxvirus infections have been confirmed in 46 animals by at least one of the laboratory tests employed: neutralizing antibody detection using as antigen a vaccinia-like strain molecularly characterized and isolated in the area; RT-PCR test; electron microscopy observation; and virus isolation in a Vero cell line. The virus strains obtained were identified as vaccinia-like by electron microscopy and reverse transcriptase polymerase chain reaction (RT-PCR), confirming the circulation of these strains in nature. Human cases associated with the infected animals have been also observed in the region, characterizing an expanding zoonosis caused by vaccinia-like strains in the state of Rio de Janeiro. DOI: http://dx.doi.org/10.17525/vrr.v12i1-2.10","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"12 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2007-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67514230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"MOLECULAR CHARACTERIZATION OF PARTIAL SEQUENCES OF BRAZILIAN BLV GAG SEQUENCES","authors":"M. F. Camargos, J. Reis, R. Leite","doi":"10.17525/VRR.V12I1-2.9","DOIUrl":"https://doi.org/10.17525/VRR.V12I1-2.9","url":null,"abstract":"PCR amplification and sequencing of gag gene fragments of two Brazilian strains of BLV were performed. After comparison of the Brazilian sequences with four sequences originating from other countries, available in the GenBank, a high conservation of this gene was observed and the results confirmed that two of the available sequences in the GenBank had sequencing errors. DOI: http://dx.doi.org/10.17525/vrr.v12i1-2.9","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"12 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2007-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67515201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Lerer, M. R. Pereira, Patricia Ormiga, F. Palma, G. F. Silva, C. Rito, V. Flores, H. Schatzmayr
{"title":"VESICOPUSTULAR FACIAL INFECTION RELATED TO POXVIRUS","authors":"C. Lerer, M. R. Pereira, Patricia Ormiga, F. Palma, G. F. Silva, C. Rito, V. Flores, H. Schatzmayr","doi":"10.17525/VRRJOURNAL.V12I1-2.14","DOIUrl":"https://doi.org/10.17525/VRRJOURNAL.V12I1-2.14","url":null,"abstract":"","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"12 1","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2007-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Costa, Bruno R. Simonetti, D. C. Abreu, J. Simonetti, M. C. Gonçalves, M. Silva, L. C. Brust, O. Barth, H. Schatzmayr
{"title":"ANIMAL INFECTIONS BY VACCINIA-LIKE VIRUSES IN THE STATE OF RIO DE JANEIRO: 2 - PARAIBA RIVER VALLEY","authors":"R. Costa, Bruno R. Simonetti, D. C. Abreu, J. Simonetti, M. C. Gonçalves, M. Silva, L. C. Brust, O. Barth, H. Schatzmayr","doi":"10.17525/VRRJOURNAL.V12I1-2.11","DOIUrl":"https://doi.org/10.17525/VRRJOURNAL.V12I1-2.11","url":null,"abstract":"Animal infections caused by vaccinia-like viruses strains in the Paraiba river valley, in the state of Rio de Janeiro, municipalities of Valenca, Barra do Pirai, Rio das Flores, Pirai, Rio Claro e Resende, have been studied by our group. Human infections associated with the infected animals have been also observed in the area. Specimens of 102 animals were collected in 34 farms in the period May 2006 to October 2007, mainly serum samples and also crusts and vesicular fluids. The clinical specimens were submitted to a plaque reduction neutralization test, reverse transcriptase polymerase chain reaction (RT-PCR) test, electron microscopy examination and virus isolation in a Vero cell line. Orthopoxvirus infection could be demonstrated by at least one of the laboratory methods in 92 animals, and lesions were observed on the udder of dairy cattle and around mouth and nose of calves in contact with infected adults. The orthopoxvirus infections are spreading in the area and may be already considered a important zoonosis, with substantial economic losses in the dairy cattle due to reduction of milk production and permanent lesions in the udder, like losses of teats. DOI: http://dx.doi.org/10.17525/vrrjournal.v12i1-2.11","PeriodicalId":30621,"journal":{"name":"Virus Reviews Research","volume":"12 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2007-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67516319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}