JuliusKuhnArchiv最新文献

{"title":"Impact of an Oomen feeding with a neonicotinoid on daily activity and colony development of honeybees assessed with an AI based monitoring device.","authors":"Gundula Gonsior, F. Tausch, Katharina Schmidt, S. Knäbe","doi":"10.5073/JKA.2020.465.006","DOIUrl":"https://doi.org/10.5073/JKA.2020.465.006","url":null,"abstract":"Feeding experiments are standard tools in the pollinator risk assessment. The design (Oomen et al. 1992) was developed to test insect growth regulators and herbicides. In recent years there was an update (Luckmann & Schmitzer 2015) on the outline in order to also focus on the advantage of different rates making a dose response design possible where exposure levels are known. Additionally, this design gives the possibility to test different rates for honey bee colonies foraging in the same landscape. \u0000The main objective of the experiment presented here was to determine the natural variability of foragers losses of hives fed with a sub-lethal neonicotinoid concentration compared to an untreated control. Other objectives were to see if the neurotoxic exposure results in any observable sub-lethal effects and to find out if losses can be correlated to hive development. This was assessed with traditional methods and a novel, visual monitoring device.","PeriodicalId":30493,"journal":{"name":"JuliusKuhnArchiv","volume":"1 1","pages":"25-29"},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49532638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disturbed energy metabolism after neonicotinoid exposure as cause of altered homing flight activity of honey bees","authors":"V. Christen, L. Jeker","doi":"10.5073/JKA.2020.465.003","DOIUrl":"https://doi.org/10.5073/JKA.2020.465.003","url":null,"abstract":"Neonicotinoids are implicated in the decline of honey bee populations. As nicotinic acetylcholine receptor agonists they disturb acetylcholine receptor signalling, leading to neurotoxicity. Several behavioural studies have shown links between neonicotinoid exposure and adverse effects on foraging activity, homing flight performance and reproduction but the molecular aspects underlying these effects are not well understood. We have elucidated the link between homing flight performance and expression of selected transcripts in the brain of honey bees. Besides possible neurotoxic effects of neonicotinoids leading to disturbed orientation and therefore prolongation of homing flight time, neonicotinoids may also disturb energy metabolism, also causing longer homing flight time. To test the second hypothesis, pollen foragers were fitted with RFID chips, exposed to 1 ng/bee thiamethoxam in single bee feeding and 10 bee-feeding settings and released 1km from the hive. The homing flight time was monitored. In the evening, all returned foragers were collected and stored at -80°C until further analysis. After homing flight data analysis, brain RNA of fast returning controls and slow returning exposed foragers of both feeding strategies was isolated and energy metabolism transcript expression was analysed using quantitative PCR. We analysed expression of cox 5a, cox 5b, cox 6c and cox 17, all transcripts of complex IV and ndufb-7, part of complex I of the oxidative phosphorylation. Comparing all generated expression data demonstrated that data of the 1 bee-feeding approach scatter less than data of the 10 bee-feeding approach. This finding clearly shows the unequal distribution of sugar syrup between caged honey bees due to trophallaxis. In addition, no significant changes were seen for all analysed transcripts of the 10 bee-feeding approach due to strong scattering of data and small sample size. In contrast, the expression of cox 5a and cox 17 was significantly altered in foragers exposed to 1 ng/bee thiamethoxam in the single bee feeding approach and there was a strong correlation between the down-regulation of cox 17 and the prolongation of homing flight time. In summary, this small study has two major findings. First, feeding strategy is very important as regards significant effects and single bee feeding approach should be used in future studies. Second, there is a clear link between prolongation of homing flight time and energy metabolism. Therefore, longer homing flight time may be not only due to disturbed orientation but also due to a lack of energy. Further studies are needed to analyse this point in more detail. 1.4 Gene expreslsion analysis in honey bees as novel tool for assessing effects of plant protection products Karl Fent1, Verena Christen1, Petra Kunz2 1University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Langackerstrasse 30, CH-4132 Muttenz, Switzerland, 2Federal Office for the Environment, CH-3003 Bern, E-Mai","PeriodicalId":30493,"journal":{"name":"JuliusKuhnArchiv","volume":"1 1","pages":"19-19"},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45849305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consequences of a short term, sub lethal pesticide exposure early in life on survival and immunity in the honeybee ( Apis mellifera )","authors":"Yahya Al Naggar, B. Baer","doi":"10.5073/JKA.2020.465.007","DOIUrl":"https://doi.org/10.5073/JKA.2020.465.007","url":null,"abstract":"","PeriodicalId":30493,"journal":{"name":"JuliusKuhnArchiv","volume":"1 1","pages":"30-30"},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44972395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conservation and creation of multi-functional margins to maintain and increase the pollinator biodiversity in agricultural environments (d)","authors":"F. J. Peris-Felipo, Oscar Aguado-Martín, Luis Miranda-Barroso","doi":"10.5073/JKA.2020.465.013","DOIUrl":"https://doi.org/10.5073/JKA.2020.465.013","url":null,"abstract":"","PeriodicalId":30493,"journal":{"name":"JuliusKuhnArchiv","volume":"1 1","pages":"33-34"},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46337568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How does the novel insecticide flupyradifurone affect honeybee longevity and behavior","authors":"R. Schreiner, A. Schuhmann, Hannah Hesselbach","doi":"10.5073/JKA.2020.465.008","DOIUrl":"https://doi.org/10.5073/JKA.2020.465.008","url":null,"abstract":"","PeriodicalId":30493,"journal":{"name":"JuliusKuhnArchiv","volume":"1 1","pages":"30-31"},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44546042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The homing flight method to assess the effect of sublethal doses of plant protection products on the honey bee in field conditions: results of the ring tests","authors":"Julie Fourrier, C. Moreau-Vauzelle, Colombe Chevallerau, P. Aupinel, Mickaël Henry, Cyril Vidau, A. Decourtye","doi":"10.5073/JKA.2020.465.002","DOIUrl":"https://doi.org/10.5073/JKA.2020.465.002","url":null,"abstract":"","PeriodicalId":30493,"journal":{"name":"JuliusKuhnArchiv","volume":"1 1","pages":"18-18"},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47475169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress on the Osmia acute oral test - findings of the ICPPR Non- Apis subgroup solitary bee laboratory testing","authors":"I. Roessink, Nicole Hanewald, Christof W. Schneider, Anja Quambusch, Nina Exeler, A. Cabrera, A. Molitor, Verena Tänzler, Bettina Hodapp, M. Albrecht, Annely Brandt, S. Vinall, Anne-Kathrin Rathke, H. Giffard, Eugenia Soler, Alexander Schnurr, Michael Patnaude, Elodie Couture, David E. Lehman","doi":"10.5073/JKA.2020.465.017","DOIUrl":"https://doi.org/10.5073/JKA.2020.465.017","url":null,"abstract":"s: Oral Presentation 46 Julius-Kühn-Archiv, 465, 2020 2.2 Progress on the Osmia acute oral test findings of the ICPPR Non-Apis subgroup solitary bee laboratory testing Ivo Roessink1, Nicole Hanewald2, Christof Schneider2, Anja Quambusch3, Nina Exeler3, Ana R. Cabrera4, AnnaMaria Molitor5, Verena Tänzler6, Bettina Hodapp7, Matthias Albrecht8, Annely Brandt9, Steven Vinall10, Anne-Kathrin Rathke11, Hervé Giffard12, Eugenia Soler13, Alexander Schnurr14, Michael Patnaude15, Elodie Couture16, David Lehman17 1Wageningen Environmental Research -The Netherlands, 2BASF-Germany, 3BAYER-Germany,4BAYER-United States, 5Eurofins Agroscience Services Ecotox GmbH-Germany, 6IBACON GmbH-Germany, 7Innovative Environmental Services (IES) Ltd-Switzerland, 8Agroscope-Switzerland, 9Landesbetrieb Landwirtschaft HessenGermany, 10MamboTox-United Kingdom, 11Noack laboratorien-Germany, 12TestApi-France, 13Trialcamp SLUSpain, 14Biochem Agrar GmbH-Germany, 15Smithers-United States, 16SynTech-France, 17US Environmental Protection Agency-United States DOI 10.5073/jka.2020.465.017","PeriodicalId":30493,"journal":{"name":"JuliusKuhnArchiv","volume":"1 1","pages":"46-46"},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48792352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing effects of insecticide seed treatments on pollinators in oilseed rape and maize","authors":"E. Pilling, A. Alix, O. Klein, Lea A. Franke, J. Fricke, M. Kleinhenz, H. Gätschenberger","doi":"10.5073/JKA.2020.465.012","DOIUrl":"https://doi.org/10.5073/JKA.2020.465.012","url":null,"abstract":"","PeriodicalId":30493,"journal":{"name":"JuliusKuhnArchiv","volume":"1 1","pages":"32-33"},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49458244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Higher TIER bumble bees and solitary bees recommendations for a semi-field experimental design","authors":"S. Knäbe, M. Allan, Annika Alscher, K. Amsel, C. Classen, Magdaléna Cornement, C. Elston, Nina Exeler, Lea A. Franke, M. Frommberger, H. Giffard, J. S. Guerola, S. Hecht-Rost, Bettina Hodapp, Ines S. Hotopp, Carole Jenkins, T. Jütte, Stefan Kimmel, O. Klein, Britta Kullmann, J. Lückmann, M. Persigehl, I. Roessink, Christof W. Schneider, Alexander Schnurr, Verena Tänzler, J. V. D. van der Steen","doi":"10.5073/JKA.2020.465.016","DOIUrl":"https://doi.org/10.5073/JKA.2020.465.016","url":null,"abstract":"The publication of the proposed EFSA risk assessment guidance document of plant protection products for pollinators highlighted that there are no study designs for non-Apis pollinators available. Since no official guidelines exist for semi-field testing at present, protocols were proposed by the ICPPR non-Apis working group and two years of ring-testing were conducted in 2016 and 2017 to develop a general test set-up. The ringtest design was based on the draft EFSA guidance document, OEPP/EPPO Guideline No. 170 and results of discussions regarding testing solitary bees and bumble bees during the meetings of the ICPPR non-Apis workgroup. \u0000Ring-tests were conducted with two different test organisms, one representative of a social bumble bee species (Bombus terrestris L; Hymenoptera, Apidae) and one representative of a solitary bee species (Osmia bicornis L; Hymenoptera, Megachilidae). The species are common species in Europe, commercially available and widely used for pollination services. Several laboratories participated in the higher-tier ring tests. 15 semi-field tests were conducted with bumble bees and 16 semi-field tests were done with solitary bees in 2016 and 2017. \u0000Two treatment groups were always included in the ringtests: an untreated control (water treated) and the treatment with dimethoate as a toxic reference item (optional other i.e. brood-affecting substances fenoxycarb or diflubenzuron). The toxic reference items were chosen based on their mode of action and long term experience in honey bee testing. \u0000A summary of the ringtest results will be given and the recommendations for the two semi-field test designs will be presented.","PeriodicalId":30493,"journal":{"name":"JuliusKuhnArchiv","volume":"1 1","pages":"40-45"},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46164601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ICPPR WG Semi-field and field Report and Discussion","authors":"E. Pilling, B. M. Barrett, A. Dinter, C. Scott-dupree, Reed M. Johnson, G. Lewis, Mark Miles, M. Persigehl, S. Hecht-Rost, B. Szczesniak, Verena Tänzler, Ulrich Zumkier","doi":"10.5073/JKA.2020.465.015","DOIUrl":"https://doi.org/10.5073/JKA.2020.465.015","url":null,"abstract":"The ICPPR Semi-Field/Field Testing (SF/FT) workgroup consists of several ‘writing groups’ that are focused developing technical guidance that is focused on 4 separate but related topics: 1) designing and conducting pollen and nectar residue studies, 2) conducting large scale colony feeding studies, 3) updating guidance for conducting semi-field tunnel studies, and 4) design and interpretation of full field studies with bees. What follows is the current status of each of these activities.","PeriodicalId":30493,"journal":{"name":"JuliusKuhnArchiv","volume":"1 1","pages":"37-39"},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41655035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}