Bioconjugate Chemistry最新文献_第4页

Nanomaterial Applications in Prevention and Treatment Strategies of Virus: A Review. 纳米材料在病毒防治策略中的应用综述

IF 4 2区化学

Bioconjugate Chemistry Pub Date : 2025-07-16 Epub Date: 2025-06-12 DOI: 10.1021/acs.bioconjchem.5c00170

Gao-Li Xin, Chong Zhang, Jia-Lin Ni, Yong-Kang Li, Yu Sun, Xiu-Xia He

{"title":"Nanomaterial Applications in Prevention and Treatment Strategies of Virus: A Review.","authors":"Gao-Li Xin, Chong Zhang, Jia-Lin Ni, Yong-Kang Li, Yu Sun, Xiu-Xia He","doi":"10.1021/acs.bioconjchem.5c00170","DOIUrl":"10.1021/acs.bioconjchem.5c00170","url":null,"abstract":"Virus infections pose significant threats to human health and have profound implications for the global economy. Traditional vaccines and antiviral drugs have several limitations in the management of viral infections. For example, the efficacy of vaccines diminishes following viral mutations, while antiviral medications are susceptible to the development of drug resistance. In recent years, nanomaterials have attracted considerable attention in antiviral research due to their physicochemical properties and biocompatibility. This article provides a systematic overview of the advancements in the antiviral application of nanomaterials. We provide a comprehensive overview of the antiviral effects of nanomaterials in various applications, including the enhancement of immune responses as vaccine carriers, improved sensitivity in virus detection, inhibition of viral infection by direct antiviral activity, enhancement of drug efficacy as drug delivery carriers, interruption of virus transmission by surface coating, and virus tracing to assist in the study of viral mechanisms. Challenges of nanomaterials in the antiviral research are also discussed.","PeriodicalId":29,"journal":{"name":"Bioconjugate Chemistry","volume":" ","pages":"1341-1361"},"PeriodicalIF":4.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing SARS-CoV-2 G-Quadruplexes in the Nsp3 Gene Segment by Fluorescence and ¹⁹F NMR Using a Functionalized Nucleoside Analog. 利用功能化核苷类似物的荧光和19F NMR探测SARS-CoV-2在Nsp3基因片段中的g -四联体。

IF 4 2区化学

Bioconjugate Chemistry Pub Date : 2025-07-16 DOI: 10.1021/acs.bioconjchem.5c00157

Sarupa Roy, Seergazhi G Srivatsan

{"title":"Probing SARS-CoV-2 G-Quadruplexes in the Nsp3 Gene Segment by Fluorescence and 19F NMR Using a Functionalized Nucleoside Analog.","authors":"Sarupa Roy, Seergazhi G Srivatsan","doi":"10.1021/acs.bioconjchem.5c00157","DOIUrl":"https://doi.org/10.1021/acs.bioconjchem.5c00157","url":null,"abstract":"The SARS-CoV-2 genome is a single-stranded RNA containing highly conserved G-rich segments that can fold into G-quadruplex (GQ) structures. These GQ structures take part in regulating the expression of both structural and nonstructural proteins, which are essential for viral replication and pathogenesis. Particularly, Nsp3 is one of the key nonstructural proteins responsible for the production of the replicase gene and controls the polyprotein expression required for viral replication. However, limited information is available on the structural polymorphism of the Nsp3 GQ motif and its interaction with small molecules, underscoring the need for a further detailed investigation. In this study, we utilized a dual-application environment-responsive nucleoside conjugate, 5-fluorobenzofuran-modified uridine (FBFU), to probe the complex structural features of the Nsp3 GQ-forming sequence using fluorescence and 19F nuclear magnetic resonance (NMR). The phosphoramidite substrate of the probe incorporated into the loop region is minimally perturbing and reports the formation of GQ structures and duplexes with distinct fluorescence and 19F NMR signals. Notably, the simple and effective 19F NMR approach is used to detect coexisting multiple GQ structures in the Nsp3 gene. Further, using the fluorescent component of the nucleoside analog, a method was designed to quantify the binding affinity of small-molecule ligands to the GQs. Taken together, our probe provided valuable insights into the structural diversity of the highly conserved Nsp3 G-rich region, which should aid in developing GQ binders and advance the therapeutic evaluation of this target.","PeriodicalId":29,"journal":{"name":"Bioconjugate Chemistry","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Mild Protecting-Group Free Strategy for Neoglycoconjugate Synthesis. 新糖缀合物的温和无保护基团合成策略。

IF 4 2区化学

Bioconjugate Chemistry Pub Date : 2025-07-16 Epub Date: 2025-06-23 DOI: 10.1021/acs.bioconjchem.5c00138

Princey Raju, Chunhua Dong, Craig R Garen, Michael T Woodside, Christopher W Cairo

{"title":"A Mild Protecting-Group Free Strategy for Neoglycoconjugate Synthesis.","authors":"Princey Raju, Chunhua Dong, Craig R Garen, Michael T Woodside, Christopher W Cairo","doi":"10.1021/acs.bioconjchem.5c00138","DOIUrl":"10.1021/acs.bioconjchem.5c00138","url":null,"abstract":"The synthesis of neoglycoconjugates has paved the way for the discovery of novel probes that mimic natural glycoconjugates and can provide designed research tools and therapeutics. In some cases, the target protein may not be amenable to harsh conditions; therefore, semisynthetic or chemical methods must be chosen with care. Here, we present a simple and modular chemoselective coupling strategy between an unprotected sugar and an N,O-disubstituted hydroxylamine under mild acidic conditions. This strategy removes any need for protecting groups on the glycan. The terminal alkene group of the conjugate serves as an effective handle to allow facile conjugation to the protein of interest via thiol-ene coupling (TEC), with proteins bearing a cysteine or free thiol to prepare neoglycoconjugates. We demonstrate that the strategy is compatible with both N- and O-linked glycans using protecting-group free strategies and optimize the TEC conditions using a variety of photocatalysts. Finally, we test the method on an aggregation-prone protein, α-synuclein. We envision that this strategy could allow the construction of complex glycoconjugates for biological testing using isolated glycans, or for generation of conjugates where the protein of interest is sensitive to harsh conditions.","PeriodicalId":29,"journal":{"name":"Bioconjugate Chemistry","volume":" ","pages":"1461-1473"},"PeriodicalIF":4.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lysosome-Targeted Naphthalimide-Based Fluorescence for the Detection of Fe(III) and Monitoring of Iron Metabolism. 溶酶体靶向萘酰亚胺荧光检测铁（III）及铁代谢监测。

IF 4 2区化学

Bioconjugate Chemistry Pub Date : 2025-07-16 Epub Date: 2025-06-11 DOI: 10.1021/acs.bioconjchem.5c00092

Shuang-Shuang Long, Xi-Feng Zou, Wen-Xi Zhang, Ke Zeng, Qing-Long Qiao, Xu-Dong Jiang, Ying-Wu Lin

{"title":"Lysosome-Targeted Naphthalimide-Based Fluorescence for the Detection of Fe(III) and Monitoring of Iron Metabolism.","authors":"Shuang-Shuang Long, Xi-Feng Zou, Wen-Xi Zhang, Ke Zeng, Qing-Long Qiao, Xu-Dong Jiang, Ying-Wu Lin","doi":"10.1021/acs.bioconjchem.5c00092","DOIUrl":"10.1021/acs.bioconjchem.5c00092","url":null,"abstract":"Iron is crucial for numerous biological processes, and lysosomes play an essential role in iron metabolism by regulating Fe3+ levels. Disruptions of this regulation can lead to Fe3+ accumulation, resulting in membrane damage and ferroptosis. Here, we have developed a water-soluble fluorescent probe BiNIT that specifically targets lysosomes for the selective detection of Fe3+. BiNIT features a bis-naphthalimide structure linked by a thiophene moiety and incorporates two quaternary ammonium groups, which enhance its ability to target lysosomes and its solubility in aqueous environments. The probe showed high selectivity for Fe3+, with fluorescence quenching resulting from the paramagnetism of Fe3+ and its capacity to induce probe aggregation. This aggregation occurs through coordination bonds between Fe3+ and the carbonyl oxygen, imide nitrogen, or thiophene sulfur in multiple probe molecules. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) confirmed the formation of nanoparticles upon Fe3+ binding. Moreover, BiNIT remains stable in environments with pH values above 4, facilitating precise monitoring of Fe3+ levels within lysosomes. This innovative tool provides valuable insights into iron homeostasis, oxidative stress, and ferroptosis, aiding research on iron-related diseases and the development of therapeutic strategies.","PeriodicalId":29,"journal":{"name":"Bioconjugate Chemistry","volume":" ","pages":"1430-1437"},"PeriodicalIF":4.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multilayer Fluorescent Immunoassay for Early and Sensitive Dengue Virus Detection Using Host and Viral Biomarkers. 利用宿主和病毒生物标志物进行登革病毒早期和敏感检测的多层荧光免疫分析。

IF 4 2区化学

Bioconjugate Chemistry Pub Date : 2025-07-16 Epub Date: 2025-06-12 DOI: 10.1021/acs.bioconjchem.5c00153

Andrew S Browne, Jieqiong Fang, Amany Elsharkawy, Tianwei Jia, Evan Reboli, Ying Luo, Xiaolin Sheng, Mukesh Kumar, Suri S Iyer

{"title":"Multilayer Fluorescent Immunoassay for Early and Sensitive Dengue Virus Detection Using Host and Viral Biomarkers.","authors":"Andrew S Browne, Jieqiong Fang, Amany Elsharkawy, Tianwei Jia, Evan Reboli, Ying Luo, Xiaolin Sheng, Mukesh Kumar, Suri S Iyer","doi":"10.1021/acs.bioconjchem.5c00153","DOIUrl":"10.1021/acs.bioconjchem.5c00153","url":null,"abstract":"Early detection and monitoring of dengue virus (DENV) infections are critical for effective disease management. A comprehensive approach combining viral and host biomarker detection improves diagnostic accuracy. Here, we describe a signal enhancement technique combining fluorescent silica nanoparticles and bioorthogonal chemistries for the ultrasensitive detection of monocyte chemoattractant protein 1 (MCP-1), interferon gamma-induced protein 10 (IP-10), and the viral biomarker nonstructural protein 1 (NS1). Our plate-based sandwich assay enhances signals with multiple layered fluorescent dye-encapsulated nanoparticles. In human serum, the assay achieved a limit of detection (LOD) of 43 pg/mL (∼5.0 nM) for MCP-1, ranging from 100 pg/mL to 100 ng/mL; 66 pg/mL (∼7.6 nM) for IP-10, ranging from 10 pg/mL to 100 ng/mL; and 351 pg/mL (8.6 nM) for NS1, ranging from 100 pg/mL to 10 μg/mL. We also monitored host biomarkers in dengue virus-infected AG129 mice using a Milliplex Mouse Cytokine/Chemokine Magnetic Bead Panel. MCP-1 levels in infected mice ranged from 1000 to 7000 pg/mL (mean: 2911 pg/mL), while uninfected controls showed much lower levels (1-10 pg/mL, mean 7 pg/mL). IP-10 levels ranged from 150 to 300 pg/mL (mean 188 pg/mL) in infected mice and 50-100 pg/mL (mean 69.4 pg/mL) in controls. These results aligned with our multilayered fluorescent assay, demonstrating its potential for sensitive dengue biomarker detection.","PeriodicalId":29,"journal":{"name":"Bioconjugate Chemistry","volume":" ","pages":"1474-1482"},"PeriodicalIF":4.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12272551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple Diaminonucleoside-Mediated Nonenzymatic Ligation of Oligonucleotides. 简单二氨基核苷介导的寡核苷酸非酶连接。

IF 4 2区化学

Bioconjugate Chemistry Pub Date : 2025-07-16 Epub Date: 2025-06-12 DOI: 10.1021/acs.bioconjchem.5c00090

Michael J Capperauld, Krish Kiran Valluru, Jagandeep S Saraya, Evan Zakaria, Connor E Wong, Derek K O'Flaherty

引用次数: 0

Near Infrared Light-Triggered Small Molecule Chemical Reactions in Biocompatible Systems. 生物相容性系统中近红外光触发的小分子化学反应。

IF 4 2区化学

Bioconjugate Chemistry Pub Date : 2025-07-16 Epub Date: 2025-06-27 DOI: 10.1021/acs.bioconjchem.5c00293

Jie Zhong, Yuhang Li, Xiaotong Li, Feng Liang, Ran Tao, Shan Qian, Xinyuan Fan

{"title":"Near Infrared Light-Triggered Small Molecule Chemical Reactions in Biocompatible Systems.","authors":"Jie Zhong, Yuhang Li, Xiaotong Li, Feng Liang, Ran Tao, Shan Qian, Xinyuan Fan","doi":"10.1021/acs.bioconjchem.5c00293","DOIUrl":"10.1021/acs.bioconjchem.5c00293","url":null,"abstract":"Near-infrared (NIR) light, within the 700-1000 nm therapeutic optical window, offers deep tissue penetration, low photocytotoxicity, and minimal side effects, making it ideal for remote control of biocompatible reactions in vivo. This review explores recent advances in NIR-triggered reactions, focusing on direct and indirect activation strategies. Direct approaches utilize NIR-responsive protecting groups, while indirect methods employ upconversion materials and photocatalysis to overcome NIR's energy limitations. These innovations expand noninvasive in vivo control capabilities. Applications include NIR-mediated drug delivery, biological molecule activation, and proximity labeling for protein interaction studies. Such reactions enable precise modulation of biological events under native conditions. The review highlights the potential of integrating advanced nanomaterials and optimizing indirect activation techniques to enhance reaction efficiency. It also emphasizes the requirement for interdisciplinary collaboration to refine NIR-responsive systems and facilitate clinical translation. By showcasing state-of-the-art NIR-controlled chemistry and identifying key areas for future research, this work aims to inspire advancements in biomedical research and therapeutics. Addressing the challenges of in vivo chemical control, this review positions NIR chemistry as a critical component in the evolution of biocompatible reaction methodologies.","PeriodicalId":29,"journal":{"name":"Bioconjugate Chemistry","volume":" ","pages":"1362-1376"},"PeriodicalIF":4.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tailoring the Methods of Conjugation and Characterization for a Replication-Competent, Live, Viral Vector. 裁剪的方法偶联和表征的复制能力，活的，病毒载体。

IF 4 2区化学

Bioconjugate Chemistry Pub Date : 2025-07-16 Epub Date: 2025-07-02 DOI: 10.1021/acs.bioconjchem.5c00060

Elise Ishida, Richard Dambra, Sally Ye, Steven Anderlot, Andrea Matter, Kaitlynn Graca, Yoo-Chun Kim, Travis Whitney, Yuecheng Xi, Leila Eslamizar, Ting Wang, Tom S Chan, Sadia Abid, Leela Kurien, Mary Sanville, Aishwarya Bapat, Theophila Dusabamahoro, Andrey Konovalov, Amy Mikolaichik, Miguel A Miranda-Roman, Audrey Brenot, Charles Wood, Birgit Fogal, Aaron M Teitelbaum, Kelly Coble, Michael Franti, Kerstin Schaefer, Joseph Ashour, Hamid Samareh Afsari

{"title":"Tailoring the Methods of Conjugation and Characterization for a Replication-Competent, Live, Viral Vector.","authors":"Elise Ishida, Richard Dambra, Sally Ye, Steven Anderlot, Andrea Matter, Kaitlynn Graca, Yoo-Chun Kim, Travis Whitney, Yuecheng Xi, Leila Eslamizar, Ting Wang, Tom S Chan, Sadia Abid, Leela Kurien, Mary Sanville, Aishwarya Bapat, Theophila Dusabamahoro, Andrey Konovalov, Amy Mikolaichik, Miguel A Miranda-Roman, Audrey Brenot, Charles Wood, Birgit Fogal, Aaron M Teitelbaum, Kelly Coble, Michael Franti, Kerstin Schaefer, Joseph Ashour, Hamid Samareh Afsari","doi":"10.1021/acs.bioconjchem.5c00060","DOIUrl":"10.1021/acs.bioconjchem.5c00060","url":null,"abstract":"Optimized functionalization of virus particles can expand the toolbox available for the development of viral vector-based therapies. However, labeling of a large, complex, and biologically active particle introduces distinct technological challenges for subsequent purification and characterization steps. Moreover, the process of labeling and purification, and even the label itself, may have an unwanted impact on the particle's biological activity. Herein, we present a comprehensive series of steps for conjugation, purification, and characterization that are adapted from traditional biologics (e.g., monoclonal antibodies) and apply them to the replication-competent rhabdovirus VSV-GP-GFP. By confirming purity and quantifying the average degree of labeling (DoL), we tailored the label/particle ratio to generate tool viruses suitable for downstream applications in nonclinical and clinical development. These methods will enhance the development of viral vector therapies and hasten their delivery to patients in need.","PeriodicalId":29,"journal":{"name":"Bioconjugate Chemistry","volume":" ","pages":"1394-1408"},"PeriodicalIF":4.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 4 2区化学

Bioconjugate Chemistry Pub Date : 2025-07-16

Hua Zhang, Tanushree Ganguly, Rebecca Harris, Ryan A. Davis, Sven H. Hausner, Luciana Kovacs and Julie L. Sutcliffe*,

引用次数: 0

IF 4 2区化学

Bioconjugate Chemistry Pub Date : 2025-07-16

Yutaro Mahara, Issa Fukuda, Ryuho Tanaka, Shin-ya Oyama, Hiroyuki Shinchi, Yasuo Suda, Nikunj M. Shukla, Michael Chan, Tomoko Hayashi, Howard B. Cottam, Dennis A. Carson and Masahiro Wakao*,

引用次数: 0