Agronomy Science and Biotechnology最新文献

{"title":"Iron concentrations in the in vitro cultivation of native Brazilian orchid Schomburgkia crispa","authors":"D. J. Bertoncelli, Guilherme Augusto Cito Alves, Gustavo Henrique Freiria, F. Furlan, H. F. I. Neto, R. T. Faria","doi":"10.33158/ASB.2018V4I2P93","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I2P93","url":null,"abstract":"In vitro cultivation is a highly important biotechnological method widely used for the production of orchid seedlings, but it is necessary to study the suitability of the nutrients used in different kinds of formulation, as the nutritional requirement varies according to the species. The objective was to evaluate different concentrations of iron in the in vitro cultivation of Schomburgkia crispa Lindl seedlings. Seedlings were obtained from seeds germinated in vitro. Modified MS culture medium was used with half of the macronutrient concentration. The micronutrients were added according to the original formulation, except for the iron which was added from a stock solution of FeEDTA (FeSO4.7H2O: 5.6 g L-1 and EDTA: 7.48 g L-1) at 0.0; 2.5; 5.0; 7.5; 10.0 and 12.5mL L-1. At 200 days after seedling transplantation, shoot height, root length, number of leaves, shoot number, leaf length, leaf width, aerial and root dry mass, chlorophyll a, b and carotenoids content were evaluated. A completely randomized design was used, with six treatments and ten replicates of five seedlings. Regression analysis was performed at 5% of significance. The increase in iron concentration caused a reduction in root length and an increase in the number of leaves and shoots. The concentration of 4.13 mL L-1 of FeEDTA was the one that provided the best in vitro growth of S. crispa plants. High concentrations of iron caused a reduction of initial development, but stimulated an increase in the number of shoots.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121151658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viability and dissemination of Pantoea ananatis, etiological agent of Maize White Spot disease","authors":"M. E. Escanferla, P. T. Wysmierski, W. F. Meirelles, L. Paccola-Meirelles","doi":"10.33158/asb.2018v4i2p52","DOIUrl":"https://doi.org/10.33158/asb.2018v4i2p52","url":null,"abstract":"Maize white spot (MWS) is a disease widely spread in maize production regions in Brazil and causes serious economic damages to the culture. Little is known about the dissemination, growth and development conditions of the MWS causal agent, the bacterium Pantoea ananatis. The objective of this work was to determine the viability of this bacterium, as well as its distribution in the plant. P. ananatis after isolated was stored in both, Nutrient Broth (NB) medium and in mineral oil. For the viability tests, the cultures stored in NB were maintained at 12ºC and –6ºC and the cultures stored in mineral oil were maintained at room temperature. The isolate remained viable for seven months in NB medium at the two temperatures assessed, and four months when stored in mineral oil. The presence of the P. ananatis bacteria was analyzed in, 1) soil samples collected close to rhizosphere, 2) in seeds from plants severely attacked by MWS, 3) foliar segments of healthy plants and 4) the stem of healthy plants. P. ananatis was found surviving epiphytically on the leaf surface and its population increased with plant age, air relative humidity, and decreasing of temperature. This agent was not found neither rhizosphere nor in seeds, but it was isolated in low quantity in maize stem. P. ananatis resides epiphytically on leaves and, due to several factors, may trigger injuries to its host. All epiphytic isolates were characterized in regards to ice nucleation activity, a characteristic of this pathogen.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133579622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplication of blueberry mini-cuttings in different growth media","authors":"R. Koyama, A. M. Assis, W. F. S. Borges, L. Yamamoto, R. Colombo, D. Zeffa, L. Barros, Beatriz Barreira, Ibrar Shahab, Saeed Ahmed, S. Roberto","doi":"10.33158/ASB.2018V4I1P28","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P28","url":null,"abstract":"The objective of this study was to evaluate the influence of length of herbaceous cuttings and substrates on the multiplication of ‘Woodard’ and ‘Briteblue’ blueberry cuttings. The experimental design was entirely randomized. Four replicates of 10 cuttings per plot in a 3x2 factorial arrangement, three cutting lengths (5, 8, and 12 cm) and two types of substrate (rice hull ash and coconut fiber) were evaluated. The cuttings were collected in two seasons: summer and fall. After the cuttings were prepared, they were placed in perforated plastic boxes containing each substrate for rooting, which were placed in a mist chamber under intermittent mist. After 158 days, the following variables were evaluated: proportion of rooted cuttings, cutting survival, leaf retention, number of roots per cutting, root mean length, root dry mass, unrooted cuttings with callus, and the proportion of sprouting cuttings. The use of 5-cm herbaceous cuttings collected in the summer and grown in rice hull optimizes the multiplication of ‘Woodard’ and ‘Briteblue’ blueberry cuttings.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125510596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of sulfosate on functional groups of microorganisms of the C and N cycles in the soybean rhizosphere","authors":"M. Avanzi, L. S. Matsumoto, U. Albino, Janaína Emiliano, G. Liuti, M. F. L. Andreata, M. Dealis, E. Niekawa, M. Navarro, G. Andrade","doi":"10.33158/ASB.2018V4I1P36","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P36","url":null,"abstract":"O uso de herbicidas no Brasil tem aumentado nos últimos anos, juntamente com a preocupação com o efeito que esses produtos podem causar no meio ambiente. O objetivo deste trabalho foi avaliar o efeito do herbicida sulfosato sobre grupos funcionais de microrganismos dos ciclos N e C na rizosfera de soja. O experimento foi conduzido em casa de vegetação, em delineamento experimental inteiramente casualizado, com cinco repetições. Os tratamentos incluíram um controle sem aplicação de herbicida e aplicação de N-fosfonometilglicina trimetilsulfônico (sulfosato) nas doses de 0,96 e 1,92 kg ha-1. Foram avaliadas as populações de bactérias heterotróficas, fungos saprófitas, actinomicetos, Pseudomonas fluorescens, celulolíticas, amilolíticas, proteolíticas e fixadoras de nitrogênio de vida livre. A massa seca de raiz e broto, número de nódulos e massa seca foram avaliados nas plantas. Na dose de 1,92 kg ha-1 de sulfosato, um efeito inibitório apareceu nas populações de fungos e actinomicetos. O herbicida sulfosato não afetou o crescimento das plantas e a nodulação em ambas as doses. A análise das correlações entre populações de microrganismos e entre populações de microrganismos e atributos de plantas mostrou diferenças entre os tratamentos herbicidas e as plantas controle.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133847745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rooting dynamics of Brazilian cherry tree minicuttings","authors":"Geniane Lopes Carvalho Ozelame, Luana Borges Affonso, Thaís Helena Cappellaro, M. Schuch, Zeni Fonseca Pinto Tomaz","doi":"10.33158/ASB.2018V4I1P46","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P46","url":null,"abstract":"The expansion of Brazilian cherry tree cultivation is affected by propagation difficulties and slow seedling production development. The cuttings and minicuttings technique is an alternative that offers several advantages, and it proved to be highly promising for many fruit tree species. Minicuttings taken from a clonal garden were rooted in 22 x 14 x 10 cm plastic boxes containing vermiculite, at IAB concentration of 2.000 mg.L-1. The experimental design was totally randomized with eight treatments (cultivation weeks), with four replications, with five minicuttings each. They were evaluated eight times (once a week), for a period of two months. The following variables were analyzed: number of swollen minicuttings, rooted minicuttings and minicuttings with roots longer than 1 cm. From the third week onwards, rooting was positive, with 70% of rooted minicuttings and 5% with swellings. In the fourth evaluation, 55% already showed roots longer than one centimeter, with a mean of 2.66 cm, five with roots emergence and three with swellings. In general, in seven weeks’ time the minicuttings showed efficient root development and were ready to be transplanted to plastic bags to develop quality seedlings.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125514106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shading levels in the development of dragon fruit (pitaya) nurseries","authors":"A. B. Lone, R. Colombo, Clandio Medeiros da Silva, Alex Takeshi Inagati, S. Roberto","doi":"10.33158/ASB.2018V4I1P8","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P8","url":null,"abstract":"O objetivo deste trabalho foi avaliar o enraizamento e brotação de estacas de pitaya (Hylocereus undatus) sob diferentes níveis de sombreamento. As tonalidades obtidas por tela foram: 37,8%, 49,6%, 86,3% e sob o sol. Foram analisadas as seguintes características: sobrevivência de estacas (%), brotação (%), número de brotações, comprimento médio dos brotos (cm), massa seca da parte aérea (g), estacas enraizadas (%), comprimento médio da raiz (cm) (mL), matéria seca da raiz (g) e concentração de clorofila aeb (mg g-1). As médias percentuais de sobrevivência foram entre 90 e 100%. As respostas para fotografar e secar a matéria seca foram quadráticas, com ponto máximo em 23,48% e 42,7% de sombreamento, respectivamente. Em relação ao enraizamento de estacas, as médias foram entre 80 e 95%. O volume radicular e a massa seca das raízes apresentaram redução linear de acordo com o nível de sombreamento. Os teores de clorofila aeb apresentaram aumento linear na função o nível de sombreamento. O sombreamento parcial (entre 23 e 42%) aumentou a porcentagem de estacas com brotações, o acúmulo de matéria seca sobre elas e o volume afetado e o acúmulo de matéria seca nas raízes de viveiros pitaya propagados por estacas.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"106 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132759824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Olive tree in vitro establishment under different culture media and explant collection periods","authors":"Roseane Maidana Moreira, M. Schuch","doi":"10.33158/ASB.2018V4I1P1","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P1","url":null,"abstract":"In the species Olea europaea L. the tissue culture techniques have not been sufficiently studied by the lack of efficient establishment of protocols for various cultivars. The objective of this work was to evaluate the effect of different culture media and sampling times of explants on in vitro establishment of six cultivars of olive (Ascolano 315, Leccino, Maria da Fé, Coratina, Arbequina and Frantoio). The work was divided into two experiments conducted in the Fruit Tree Propagation Laboratory, Crop Science Department, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, Brazil. Stock plants of the olive cultivars maintained in semi- hydroponic system were used. In the first experiment, nodal segments were used to establish in vitro culture medium MO and WPM, consisting of salts and vitamins. In the second experiment four seasons to obtain the explants were evaluate (autumn, winter, spring and summer). The WPM medium promoted greater establishment of olive explants. There is a higher phenolic oxidation in olive explants collected in winter. Spring is indicated for collecting explants, therefore favors the in vitro cultivar Maria da Fé, while Ascolano 315 and Arbequina cultivars have a higher rate of in vitro establishment by collecting explants over the year","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123497806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Storage temperature effect on seed emergence and substrates during the initial development of yellow passion fruit","authors":"Andrés Vargas, A. M. Assis, M. Nadal, M. Schuch, Vanussa Tunes","doi":"10.33158/ASB.2018V4I1P22","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P22","url":null,"abstract":"A eficácia na produção de plântulas de maracujá-amarelo (Passiflora edulis Sims e F. flavicarpa Deg) pode ser afetada pela germinação irregular, devido, principalmente, a um curto período de dormência das sementes, bem como ao uso do substrato errado. Assim, o objetivo deste trabalho foi avaliar o efeito de três temperaturas de armazenamento e três substratos durante o desenvolvimento inicial de plântulas de maracujazeiro amarelo. O experimento foi conduzido em casa de vegetação agrícola na Universidade Federal de Pelotas, RS. Após a retirada da mucilagem e secagem, as sementes foram acondicionadas em embalagens de papel Kraft e armazenadas a 5 ºC, 10 ºC e temperatura ambiente (18 ± 0,6 ºC) por 15 dias. Em seguida, eles foram cultivados em fibra de coco Amafibra®, casca de arroz carbonizada e S-10Beifort®. Sementes mantidas a 18 ºC apresentaram menor comprimento da raiz sem diferença entre os armazenados a 5 ºC. Não houve efeito da temperatura na emergência, comprimento da parte aérea, número de folhas e biomassa seca da parte aérea e das raízes. A maior porcentagem de germinação foi encontrada no substrato da casca de arroz carbonizada; no entanto, com exceção do maior comprimento radicular e da biomassa seca da parte aérea e das raízes, o desenvolvimento das plantas foi superior com os demais substratos. Assim, a utilização de temperatura de 10 ºC durante o armazenamento, bem como fibra de coco e S-10 Beifort® como substratos são as melhores recomendações para melhorar a emergência e desenvolvimento inicial do maracujá-amarelo. com exceção do maior comprimento radicular e da biomassa seca da parte aérea e das raízes, o desenvolvimento da planta foi superior com os demais substratos. Assim, a utilização de temperatura de 10 ºC durante o armazenamento, bem como fibra de coco e S-10 Beifort® como substratos são as melhores recomendações para melhorar a emergência e desenvolvimento inicial do maracujá-amarelo. com exceção do maior comprimento radicular e da biomassa seca da parte aérea e das raízes, o desenvolvimento da planta foi superior com os demais substratos. Assim, a utilização de temperatura de 10 ºC durante o armazenamento, bem como fibra de coco e S-10 Beifort® como substratos são as melhores recomendações para melhorar a emergência e desenvolvimento inicial do maracujá-amarelo.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121953024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agroclimatic zoning for winemaking grape production in the State of Parana","authors":"W. S. Ricce, S. Roberto, J. Tonietto, P. Caramori","doi":"10.33158/ASB.2018V4I1P14","DOIUrl":"https://doi.org/10.33158/ASB.2018V4I1P14","url":null,"abstract":"Paraná is the fourth largest grape producing state in Brazil and the cultivation of Vitis vinifera L. cultivars for winemaking is expanding is several regions of the state. The objective of this work was to characterize the potential of wine grape production based on the Géoviticulture Multicriteria Climatic Classification System for Paraná. A 30-year database constituted of 21 IAPAR (Agronomic Institute of Paraná) meteorological stations and 455 rainfall stations from Instituto das Águas do Paraná (Paraná State Water Institute) generated the following climatic indexes: dryness index (DI), heliothermal index (HI) and cool night index (CI) for the periods of October to March and April to September. According to the results, the viticultural climate of some regions in the state of Paraná belongs to the climatic groups where are found several traditional wine-producing regions in the world, showing potential for the expansion of winemaking in the state. Viticultural climate, associated with latitude in Paraná and with thermal conditions for vine growing cycle all over the year, make production displacement possible in the West, North and Northeastern regions and the production of the best quality grapes for winemaking in the fall and winter period, due to the most favorable cold night index and the lowest rainfall volume. In the coldest regions of the state (Center, South and East), it is only possible to have one production cycle, since the risk of frosts prevents the exploration of grapes in different periods.","PeriodicalId":297313,"journal":{"name":"Agronomy Science and Biotechnology","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132151945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}