Dhiraj Dokwal, Philip M. Brown, Karolina Filipowska and Kimberly A. Reynolds*,
{"title":"Considering Metabolic Context in Enzyme Evolution and Design","authors":"Dhiraj Dokwal, Philip M. Brown, Karolina Filipowska and Kimberly A. Reynolds*, ","doi":"10.1021/acs.biochem.5c00165","DOIUrl":"10.1021/acs.biochem.5c00165","url":null,"abstract":"<p >Enzymes are often treated as isolated molecular entities when measuring their biochemical activity and designing synthetic sequences. Yet inside the cell, enzymes interact through shared metabolite pools, physical binding, and regulatory feedback. This coordination is necessary for responsive, cohesive metabolic behavior. Moreover, these interactions place constraints on enzyme activity, specificity, abundance, and ultimately sequence. Defining these interaction-mediated constraints is important to understand metabolic evolution and to design synthetic systems. For example, the influence of the cellular and environmental milieu becomes evident when a heterologous enzyme from one species fails to function properly upon transfer to a new host. In this review we consider how cell context shapes enzyme evolution, and in turn, how variations in enzyme biochemistry, biophysics, and abundance impact cell fitness.</p>","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":"64 16","pages":"3495–3507"},"PeriodicalIF":3.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144787793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Patrick Hewitt, Julian Seidel, Anja Wüst, Meghan Smith, Stephanie J. Maiocco, Stephanie Shternberg, Maren Hoffmann, Thomas Spatzal, Stefan Gerhardt, Oliver Einsle* and Sean J. Elliott*,
{"title":"","authors":"Patrick Hewitt, Julian Seidel, Anja Wüst, Meghan Smith, Stephanie J. Maiocco, Stephanie Shternberg, Maren Hoffmann, Thomas Spatzal, Stefan Gerhardt, Oliver Einsle* and Sean J. Elliott*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":"64 15","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acs.biochem.5c00202","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144770474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dominik E. Künzler, Luca Bressan, Linda Jäger, Marianne Gamper and Peter Kast*,
{"title":"","authors":"Dominik E. Künzler, Luca Bressan, Linda Jäger, Marianne Gamper and Peter Kast*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":"64 15","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acs.biochem.5c00157","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144770483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aishat Alatishe, Therese Albert, Cameron Christopher Lee-Lopez, Rashedul Hasan, Pierre Moënne-Loccoz, Kelly N. Chacón and Erik T. Yukl*,
{"title":"","authors":"Aishat Alatishe, Therese Albert, Cameron Christopher Lee-Lopez, Rashedul Hasan, Pierre Moënne-Loccoz, Kelly N. Chacón and Erik T. Yukl*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":"64 15","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acs.biochem.5c00262","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144770492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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