Biochemistry Biochemistry最新文献_第4页

Fragment-Based Interrogation of the 14-3-3/TAZ Protein-Protein Interaction. 基于片段的 14-3-3/TAZ蛋白-蛋白相互作用研究

IF 2.9 3区生物学

Biochemistry Biochemistry Pub Date : 2024-09-03 Epub Date: 2024-08-22 DOI: 10.1021/acs.biochem.4c00248

Blaž Andlovic, Dario Valenti, Federica Centorrino, Francesca Picarazzi, Stanimira Hristeva, Malgorzata Hiltmann, Alexander Wolf, François-Xavier Cantrelle, Mattia Mori, Isabelle Landrieu, Laura M Levy, Bert Klebl, Dimitrios Tzalis, Thorsten Genski, Jan Eickhoff, Christian Ottmann

引用次数: 0

Deciphering the Molecular Dance: Exploring the Dynamic Interplay Between Mouse Insulin B9–23 Peptides and their Variants 解密分子舞蹈：探索小鼠胰岛素 B9-23 肽及其变体之间的动态相互作用

IF 2.9 3区生物学

Biochemistry Biochemistry Pub Date : 2024-09-02 DOI: 10.1021/acs.biochem.4c0021710.1021/acs.biochem.4c00217

Aleksandra Antevska, Kayla A. Hess, Connor C. Long, Ethan J. Walker, Joshua H. Jang, Riellie J. DeSoto, Kristi L. Lazar Cantrell, Lauren E. Buchanan* and Thanh D. Do*,

{"title":"Deciphering the Molecular Dance: Exploring the Dynamic Interplay Between Mouse Insulin B9–23 Peptides and their Variants","authors":"Aleksandra Antevska, Kayla A. Hess, Connor C. Long, Ethan J. Walker, Joshua H. Jang, Riellie J. DeSoto, Kristi L. Lazar Cantrell, Lauren E. Buchanan* and Thanh D. Do*, ","doi":"10.1021/acs.biochem.4c0021710.1021/acs.biochem.4c00217","DOIUrl":"https://doi.org/10.1021/acs.biochem.4c00217https://doi.org/10.1021/acs.biochem.4c00217","url":null,"abstract":"Type 1 diabetes results from the autoimmune destruction of pancreatic insulin-producing β-cells, primarily targeted by autoreactive T cells that recognize insulin B9–23 peptides as antigens. Using drift tube ion mobility spectrometry-mass spectrometry, transmission electron microscopy, and two-dimensional infrared spectroscopy, we characterized mouse insulin 1 B9–23 (Ins1 B9–23), insulin 2 B9–23 (Ins2 B9–23), along with two of their mutants, Ins2 B9–23 Y16A and Ins2 B9–23 C19S. Our findings indicate that Ins1 B9–23 and the Ins2 Y16A mutant exhibit rapid fibril formation, whereas Ins2 B9–23 and the Ins2 C19S mutant show slower fibrillization and a structural rearrangement from globular protofibrils to fibrillar aggregates. These differences in aggregation behaviors also manifest in interactions with (−)epigallocatechin gallate (EGCG), a canonical amyloid inhibitor. EGCG effectively disrupts the fibrils formed by Ins1 B9–23 and the Y16A mutant. However, it proves ineffective in preventing fibril formation of Ins2 B9–23 and the C19S mutant. These results establish a strong correlation between the aggregation behaviors of these peptides and their divergent effects on anti-islet autoimmunity.","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142237882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Helical Domain Changes between hGBP3 and hGBP3ΔC Result in Distinct Oligomers and Anti-HCV Activity. hGBP3 和 hGBP3ΔC 之间的螺旋结构变化导致不同的低聚物和抗HCV 活性。

IF 2.9 3区生物学

Biochemistry Biochemistry Pub Date : 2024-08-30 DOI: 10.1021/acs.biochem.4c00332

Sowmiya Gupta, Aunji Pradhan, Divya Rashmi, Monika Mittal, Saumitra Das, Apurba Kumar Sau

{"title":"Helical Domain Changes between hGBP3 and hGBP3ΔC Result in Distinct Oligomers and Anti-HCV Activity.","authors":"Sowmiya Gupta, Aunji Pradhan, Divya Rashmi, Monika Mittal, Saumitra Das, Apurba Kumar Sau","doi":"10.1021/acs.biochem.4c00332","DOIUrl":"https://doi.org/10.1021/acs.biochem.4c00332","url":null,"abstract":"Human guanylate binding proteins (hGBPs), which are large GTPases, are crucial for cell-autonomous immunity, including antiviral activity. hGBPs contain two domains: an N-terminal catalytic domain and a C-terminal helical domain. hGBP3 and its splice variant hGBP3ΔC have been shown to possess anti-influenza activity in lung epithelial cells. These two proteins have identical catalytic domains but different helical domains. It is unclear whether this difference affects GTPase activity or protein oligomerization. Using combined approaches, we show that both proteins hydrolyze GTP to GDP and further to GMP. However, they form different oligomers. hGBP3 exists as a hexamer in the free form, whereas hGBP3ΔC forms large oligomers, indicating that helical domain modifications of the splice variant result in distinct oligomers. Furthermore, unlike other homologues, neither protein changes its oligomeric state upon substrate binding or hydrolysis. Deleting the helical domain of hGBP3 (hGBP31-309) yields a monomer, suggesting that the helical domain promotes the hexamerization of hGBP3. We overexpressed hGBP3 and hGBP3ΔC to test their efficacy against HCV growth and found that hGBP3 inhibits HCV multiplication, while the splice variant has little effect. Our mutational studies on hGBP3 show that substrate hydrolysis, rather than substrate binding, is required for inhibiting HCV growth. This suggests that substrate hydrolysis generates a protein conformation essential for anti-HCV activity. Additionally, truncated hGBP31-309 does not exhibit anti-HCV activity. Altogether, these findings suggest that the helical domain of hGBP3 is crucial for reducing HCV growth through hexamer formation and that its variations result in different oligomers and antiviral activities.","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142102150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polar Interactions between Substrate and Flavin Control Iodotyrosine Deiodinase Function 底物与黄素之间的极性相互作用控制着碘酪氨酸脱碘酶的功能

IF 2.9 3区生物学

Biochemistry Biochemistry Pub Date : 2024-08-30 DOI: 10.1021/acs.biochem.4c0035710.1021/acs.biochem.4c00357

Daniel Lemen, and , Steven E. Rokita*,

{"title":"Polar Interactions between Substrate and Flavin Control Iodotyrosine Deiodinase Function","authors":"Daniel Lemen,  and , Steven E. Rokita*, ","doi":"10.1021/acs.biochem.4c0035710.1021/acs.biochem.4c00357","DOIUrl":"https://doi.org/10.1021/acs.biochem.4c00357https://doi.org/10.1021/acs.biochem.4c00357","url":null,"abstract":"Flavin cofactors offer a wide range of chemical mechanisms to support a great diversity in catalytic function. As a corollary, such diversity necessitates careful control within each flavoprotein to limit its function to an appropriate subset of possible reactions and substrates. This task falls to the protein environment surrounding the flavin in most enzymes. For iodotyrosine deiodinase that catalyzes a reductive dehalogenation of halotyrosines, substrates can dictate the chemistry available to the flavin. Their ability to stabilize the necessary one-electron reduced semiquinone form of flavin strictly depends on a direct coordination between the flavin and α-ammonium and carboxylate groups of its substrates. While perturbations to the carboxylate group do not significantly affect binding to the resting oxidized form of the deiodinase, dehalogenation (kcat/Km) is suppressed by over 2000-fold. Lack of the α-ammonium group abolishes detectable binding and dehalogenation. Substitution of the ammonium group with a hydroxyl group does not restore measurable binding but does support dehalogenation with an efficiency greater than those of the carboxylate derivatives. Consistent with these observations, the flavin semiquinone does not accumulate during redox titration in the presence of inert substrate analogues lacking either the α-ammonium or carboxylate groups. As a complement, a nitroreductase activity based on hydride transfer is revealed for the appropriate substrates with perturbations to their zwitterion.","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142237937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Electron-Transfer Chain to a Flavodiiron Protein in Fusobacterium nucleatum Couples Butyryl-CoA Oxidation to O2 Reduction 核酸镰刀菌中黄酮铁蛋白的新电子传递链将丁酰-CoA 氧化与 O2 还原耦合在一起

IF 2.9 3区生物学

Biochemistry Biochemistry Pub Date : 2024-08-29 DOI: 10.1021/acs.biochem.4c0027910.1021/acs.biochem.4c00279

Liam T. Bystrom, and , Kirsten R. Wolthers*,

{"title":"New Electron-Transfer Chain to a Flavodiiron Protein in Fusobacterium nucleatum Couples Butyryl-CoA Oxidation to O2 Reduction","authors":"Liam T. Bystrom,  and , Kirsten R. Wolthers*, ","doi":"10.1021/acs.biochem.4c0027910.1021/acs.biochem.4c00279","DOIUrl":"https://doi.org/10.1021/acs.biochem.4c00279https://doi.org/10.1021/acs.biochem.4c00279","url":null,"abstract":"Fusobacterium nucleatum, a Gram-negative obligate anaerobe, is common to the oral microbiota, but the species is known to infect other sites of the body where it is associated with a range of pathologies. At present, little is known about the mechanisms by which F. nucleatum mitigates against oxidative and nitrosative stress. Inspection of the F. nucleatum subsp. polymorphum ATCC 10953 genome reveals that it encodes a flavodiiron protein (FDP; FNP2073) that is known in other organisms to reduce NO to N2O and/or O2 to H2O. FNP2073 is dicistronic with a gene encoding a multicomponent enzyme termed BCR for butyryl-CoA reductase. BCR is composed of a butyryl-CoA dehydrogenase domain (BCD), the C-terminal domain of the α-subunit of the electron-transfer flavoprotein (Etfα), and a rubredoxin domain. We show that BCR and the FDP form an α4β4 heterotetramic complex and use butyryl-CoA to selectively reduce O2 to H2O. The FAD associated with the Etfα domain (α-FAD) forms red anionic semiquinone (FAD•–), whereas the FAD present in the BCD domain (δ-FAD) forms the blue-neutral semiquinone (FADH•), indicating that both cofactors participate in one-electron transfers. This was confirmed in stopped-flow studies where the reduction of oxidized BCR with an excess of butyryl-CoA resulted in rapid (<1.6 ms) interflavin electron transfer evidenced by the formation of the FAD•–. Analysis of bacterial genomes revealed that the dicistron is present in obligate anaerobic gut bacteria considered to be beneficial by virtue of their ability to produce butyrate. Thus, BCR-FDP may help to maintain anaerobiosis in the colon.","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142237611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole Cell Luminescence-Based Screen for Inhibitors of the Bacterial Sec Machinery 基于全细胞发光筛选细菌 Sec 机制抑制剂

IF 2.9 3区生物学

Biochemistry Biochemistry Pub Date : 2024-08-29 DOI: 10.1021/acs.biochem.4c0026410.1021/acs.biochem.4c00264

Tia Salter, Ian Collinson* and William J. Allen*,

{"title":"Whole Cell Luminescence-Based Screen for Inhibitors of the Bacterial Sec Machinery","authors":"Tia Salter, Ian Collinson* and William J. Allen*, ","doi":"10.1021/acs.biochem.4c0026410.1021/acs.biochem.4c00264","DOIUrl":"https://doi.org/10.1021/acs.biochem.4c00264https://doi.org/10.1021/acs.biochem.4c00264","url":null,"abstract":"There is a pressing need for new antibiotics to combat rising resistance to those already in use. The bacterial general secretion (Sec) system has long been considered a good target for novel antimicrobials thanks to its irreplacable role in maintaining cell envelope integrity, yet the lack of a robust, high-throughput method to screen for Sec inhibition has so far hampered efforts to realize this potential. Here, we have adapted our recently developed in vitro assay for Sec activity─based on the split NanoLuc luciferase─to work at scale and in living cells. A simple counterscreen allows compounds that specifically target Sec to be distinguished from those with other effects on cellular function. As proof of principle, we have applied this assay to a library of 5000 compounds and identified a handful of moderately effective in vivo inhibitors of Sec. Although these hits are unlikely to be potent enough to use as a basis for drug development, they demonstrate the efficacy of the screen. We therefore anticipate that the methods presented here will be scalable to larger compound libraries, in the ultimate quest for Sec inhibitors with clinically relevant properties.","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acs.biochem.4c00264","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142237625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Design of High Affinity Interaction Between CC Chemokine Binding Protein vCCI and CCL17/TARC 合理设计 CC 趋化因子结合蛋白 vCCI 与 CCL17/TARC 之间的高亲和力相互作用

IF 2.9 3区生物学

Biochemistry Biochemistry Pub Date : 2024-08-28 DOI: 10.1021/acs.biochem.4c0029810.1021/acs.biochem.4c00298

Wenyan Guan, Lauren E. Stark, Ning Zhang, Arjan Bains, Airam Martinez, Cynthia M. Dupureur, Michael E. Colvin and Patricia J. LiWang*,

{"title":"Rational Design of High Affinity Interaction Between CC Chemokine Binding Protein vCCI and CCL17/TARC","authors":"Wenyan Guan, Lauren E. Stark, Ning Zhang, Arjan Bains, Airam Martinez, Cynthia M. Dupureur, Michael E. Colvin and Patricia J. LiWang*, ","doi":"10.1021/acs.biochem.4c0029810.1021/acs.biochem.4c00298","DOIUrl":"https://doi.org/10.1021/acs.biochem.4c00298https://doi.org/10.1021/acs.biochem.4c00298","url":null,"abstract":"The poxvirus-derived protein vCCI (viral CC chemokine inhibitor) binds almost all members of the CC chemokine family with nanomolar affinity, inhibiting their pro-inflammatory actions. Understanding the affinity and specificity of vCCI could lead to new anti-inflammatory therapeutics. CCL17, also known as TARC, is unusual among CC chemokines by having only micromolar binding to vCCI. We have used sequence analysis and molecular simulations to determine the cause of this weak binding, which identified several locations in CCL17 where mutations seemed likely to improve binding to vCCI. Based on the aforementioned analysis, we expressed and tested multiple mutants of CCL17. We found two single point mutants V44K and Q45R that increased binding affinity to vCCI by 2–3-fold and, in combination, further improved affinity by 7-fold. The CCL17 triple mutant G17R/V44K/Q45R yielded a Kd of 0.25 ± 0.13 μM, a 68-fold improvement in affinity compared to the complex with wild-type CCL17. A quadruple mutant G17R/V44K/Q45R/R57W showed high affinity (0.59 ± 0.09 μM) compared to the wild type but lower affinity than the triple mutant. This work demonstrates that sequence comparisons and molecular simulations can predict chemokine mutations that increase the level of binding to vCCI, an important first step in developing engineered chemokine inhibitors useful for anti-inflammatory therapy.","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acs.biochem.4c00298","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142237855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Glycosyltransferases from Campylobacter concisus Diverge from the Canonical Campylobacter N-Linked Glycan Assembly Pathway 康氏弯曲杆菌的双糖基转移酶与典型弯曲杆菌 N-连接糖组装途径存在差异

IF 2.9 3区生物学

Biochemistry Biochemistry Pub Date : 2024-08-28 DOI: 10.1021/acs.biochem.4c0035110.1021/acs.biochem.4c00351

Christine A. Arbour, Nemanja Vuksanovic, Karen N. Allen* and Barbara Imperiali*,

{"title":"Dual Glycosyltransferases from Campylobacter concisus Diverge from the Canonical Campylobacter N-Linked Glycan Assembly Pathway","authors":"Christine A. Arbour, Nemanja Vuksanovic, Karen N. Allen* and Barbara Imperiali*, ","doi":"10.1021/acs.biochem.4c0035110.1021/acs.biochem.4c00351","DOIUrl":"https://doi.org/10.1021/acs.biochem.4c00351https://doi.org/10.1021/acs.biochem.4c00351","url":null,"abstract":"Species within the Campylobacter genus are recognized as emerging human pathogens. Common to all known members of the genus is the presence of an asparagine-linked glycosylation pathway encoded by the pgl operon. Campylobacter species are divided into two major groups, Group I and Group II. To date, most biochemical studies have focused on the Group I species including Campylobacter jejuni. We recently reported that the Group II Campylobacter concisus pathway deviates from that of Group I by the inclusion of a C-6″-oxidized GalNAc (GalNAcA) at the third position installed by PglJ. Herein, we investigate the diversification of the PglH enzymes that act subsequent to installation of GalNAcA. The majority of pgl operons from Group II species, including C. concisus, encode two GT-B fold glycosyltransferases (GTs), PglH1 and PglH2. As the functions of these GTs were not clear by simple comparison of their sequences to that of C. jejuni PglH, further analyses were required. We show that subsequent to the action of PglJ, PglH2 installs the next HexNAc followed by PglH1 adding a single sugar. These steps diverge from the C. jejuni pathway not only in the identity of the sugar donors (UDP-GlcNAc) but also in installing single sugars rather than acting processively. These biochemical studies were extended via bioinformatics to identify sequence signatures that provide predictive capabilities for unraveling the prokaryotic glycan landscape. Phylogenetic analysis showed early divergence between the C. jejuni PglH orthologs and C. concisus PglH1/PglH2 orthologs, leading to diversification of the final glycan.","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142237915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulfotransferase 1C2 Increases Mitochondrial Respiration by Converting Mitochondrial Membrane Cholesterol to Cholesterol Sulfate 硫基转移酶 1C2 通过将线粒体膜胆固醇转化为胆固醇硫酸盐来提高线粒体的呼吸作用

IF 2.9 3区生物学

Biochemistry Biochemistry Pub Date : 2024-08-28 DOI: 10.1021/acs.biochem.3c0034410.1021/acs.biochem.3c00344

Alexander J. Kolb, Peter Corridon, Mahbub Ullah, Zechariah J. Pfaffenberger, Wei Min Xu, Seth Winfree, Ruben H. Sandoval, Takeshi Hato, Frank A. Witzmann, Rodrigo Mohallem, Jackeline Franco, Uma K. Aryal, Simon J. Atkinson, David P. Basile and Robert L. Bacallao*,

{"title":"Sulfotransferase 1C2 Increases Mitochondrial Respiration by Converting Mitochondrial Membrane Cholesterol to Cholesterol Sulfate","authors":"Alexander J. Kolb, Peter Corridon, Mahbub Ullah, Zechariah J. Pfaffenberger, Wei Min Xu, Seth Winfree, Ruben H. Sandoval, Takeshi Hato, Frank A. Witzmann, Rodrigo Mohallem, Jackeline Franco, Uma K. Aryal, Simon J. Atkinson, David P. Basile and Robert L. Bacallao*, ","doi":"10.1021/acs.biochem.3c0034410.1021/acs.biochem.3c00344","DOIUrl":"https://doi.org/10.1021/acs.biochem.3c00344https://doi.org/10.1021/acs.biochem.3c00344","url":null,"abstract":"Hypothesis: In this communication, we test the hypothesis that sulfotransferase 1C2 (SULT1C2, UniProt accession no. Q9WUW8) can modulate mitochondrial respiration by increasing state-III respiration. Methods and results: Using freshly isolated mitochondria, the addition of SULT1C2 and 3-phosphoadenosine 5 phosphosulfate (PAPS) results in an increased maximal respiratory capacity in response to the addition of succinate, ADP, and rotenone. Lipidomics and thin-layer chromatography of mitochondria treated with SULT1C2 and PAPS showed an increase in the level of cholesterol sulfate. Notably, adding cholesterol sulfate at nanomolar concentration to freshly isolated mitochondria also increases maximal respiratory capacity. In vivo studies utilizing gene delivery of SULT1C2 expression plasmids to kidneys result in increased mitochondrial membrane potential and confer resistance to ischemia/reperfusion injury. Mitochondria isolated from gene-transduced kidneys have elevated state-III respiration as compared with controls, thereby recapitulating results obtained with mitochondrial fractions treated with SULT1C2 and PAPS. Conclusion: SULT1C2 increases mitochondrial respiratory capacity by modifying cholesterol, resulting in increased membrane potential and maximal respiratory capacity. This finding uncovers a unique role of SULT1C2 in cellular physiology and extends the role of sulfotransferases in modulating cellular metabolism.","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acs.biochem.3c00344","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142237867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution of Proteorhodopsin to Light-Dependent Biological Responses in Hymenobacter nivis P3T Isolated from Red Snow in Antarctica 从南极洲红雪中分离出的 Hymenobacter nivis P3T 中的蛋白光蛋白对光依赖性生物反应的贡献

IF 2.9 3区生物学

Biochemistry Biochemistry Pub Date : 2024-08-28 DOI: 10.1021/acs.biochem.4c0028610.1021/acs.biochem.4c00286

Kaori Kondo, Ryouhei Ohtake, Shunsuke Nakano, Mia Terashima, Hisaya Kojima, Manabu Fukui, Makoto Demura, Takashi Kikukawa and Takashi Tsukamoto*,

{"title":"Contribution of Proteorhodopsin to Light-Dependent Biological Responses in Hymenobacter nivis P3T Isolated from Red Snow in Antarctica","authors":"Kaori Kondo, Ryouhei Ohtake, Shunsuke Nakano, Mia Terashima, Hisaya Kojima, Manabu Fukui, Makoto Demura, Takashi Kikukawa and Takashi Tsukamoto*, ","doi":"10.1021/acs.biochem.4c0028610.1021/acs.biochem.4c00286","DOIUrl":"https://doi.org/10.1021/acs.biochem.4c00286https://doi.org/10.1021/acs.biochem.4c00286","url":null,"abstract":"Proteorhodopsin (PR) is a major family of microbial rhodopsins that function as light-driven outward proton pumps. PR is now widely recognized for its ecological importance as a molecule responsible for solar energy flow in various ecosystems on the earth. However, few concrete examples of the actual use of light by natural microorganisms via PR have been demonstrated experimentally. This study reveals one example of that in a cryophilic bacterium Hymenobacter nivis P3T isolated from red snow in Antarctica. The results demonstrate light-dependent biochemical and biological responses in H. nivis cells, such as the proton pump activity of H. nivis PR (HnPR), which leads to the production of proton motive force, cellular ATP production, and cell growth. In addition, the results of this study demonstrate the photochemical properties of a PR, namely, HnPR, in the membrane of a natural host bacterium. The photocycle of HnPR was much faster than other PRs even at 5 °C, indicating that the proton pump function of HnPR has adapted to the low-temperature environment of Antarctica. Although it is well-known that PR helps natural host microorganisms to use light energy, this study provides another concrete example for understanding the biological role of PR by demonstrating the link between the molecular functions of PR and the light-dependent biochemical and biological responses of a PR-bearing host.","PeriodicalId":28,"journal":{"name":"Biochemistry Biochemistry","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142237866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0