BiochemRN: Other Biochemistry Methods (Topic)最新文献

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A Fast Genetically Encoded Fluorescent Sensor for Faithful in vivo Acetylcholine Detection in Mice, Fish, Worms and Flies 一种快速基因编码荧光传感器在小鼠、鱼类、蠕虫和苍蝇体内忠实检测乙酰胆碱

BiochemRN: Other Biochemistry Methods (Topic) Pub Date : 2020-03-30 DOI: 10.2139/ssrn.3554080

P. Borden, Peng Zhang, Amol V. Shivange, J. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, O. Novák, M. Tanimoto, E. Shigetomi, Mark A. Lobas, Hyun-Tae Kim, Paula K Zhu, Yajun Zhang, W. Zheng, Chengcheng Fan, Guangfu Wang, Bowen Xiang, Lihua Gan, Guang-Xian Zhang, Kaiming Guo, Li Lin, Yuan Cai, Andrew G Yee, Abhi Aggarwal, Huan Bao, Xiaochu Lou, E. Chapman, C. P. Ford, D. Rees, D. Dietrich, B. Khakh, J. Dittman, W. Gan, Minoru Koyama, V. Jayaraman, J. Cheer, H. Lester, J. J. Zhu, L. Looger

引用次数: 3

Preparation of Boronate Affinity - Functionalized Metal-Organic Framework Material For Selective Recognition And Separation of Glycoprotein Under Physiological pH 生理pH下用于糖蛋白选择性识别和分离的硼酸盐亲和功能化金属-有机骨架材料的制备

BiochemRN: Other Biochemistry Methods (Topic) Pub Date : 1900-01-01 DOI: 10.2139/ssrn.3932111

Baoyue Zhang, Jianghua He, Bailin Guo, Xue Chen, Sheng Bi, Feng Zhang, M. Tian

{"title":"Preparation of Boronate Affinity - Functionalized Metal-Organic Framework Material For Selective Recognition And Separation of Glycoprotein Under Physiological pH","authors":"Baoyue Zhang, Jianghua He, Bailin Guo, Xue Chen, Sheng Bi, Feng Zhang, M. Tian","doi":"10.2139/ssrn.3932111","DOIUrl":"https://doi.org/10.2139/ssrn.3932111","url":null,"abstract":"Glycoproteins play an important role in biological processes such as protein folding, information transmission, nerve conduction, and molecular recognition. Therefore, it is of great significance to design and develop an adsorbent with high adsorption capacity for glycoprotein. In this paper, a novel boronate affinity material (Zr-MOF@S-S@B) was prepared by combining Zr-MOF, disulfide covalent bond (-S-S-), 4-mercaptophenylboronic acid (4-MPBA) for selective enrichment of glycoprotein and elution of glycoprotein at physiological pH. The affinity between boric acid and cis - diol makes the composite material has a high adsorption capacity of 625.5 mg g -1 for ovalbumin (OVA). At the same time, the Zr-MOF@S-S@B has exhibited great reusability and large specific surface area. Furthermore, the material contains disulfide bonds that can release surface binding glycoprotein specifically under physiological pH conditions, effectively avoiding the reduction of glycoprotein activity. In general, the method is successfully used for the enrichment of OVA in egg white samples, which has broad application prospects in the study of glycoprotein.","PeriodicalId":286484,"journal":{"name":"BiochemRN: Other Biochemistry Methods (Topic)","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130022250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Pure Curcumin Spherulites from Impure Solution Via Non-Classical Crystallization 通过非经典结晶从不纯溶液中提取纯姜黄素球粒

BiochemRN: Other Biochemistry Methods (Topic) Pub Date : 1900-01-01 DOI: 10.2139/ssrn.3844727

V. K. Kannuchamy, K. Ramisetty, K. Renukadevi, Rama Krishna Gamidi, C. Heffernan, Andrew A. Stewart, Jian Guo, Gadipelli Srinivas, D. Brett, E. Favvas, Å. Rasmuson

{"title":"Pure Curcumin Spherulites from Impure Solution Via Non-Classical Crystallization","authors":"V. K. Kannuchamy, K. Ramisetty, K. Renukadevi, Rama Krishna Gamidi, C. Heffernan, Andrew A. Stewart, Jian Guo, Gadipelli Srinivas, D. Brett, E. Favvas, Å. Rasmuson","doi":"10.2139/ssrn.3844727","DOIUrl":"https://doi.org/10.2139/ssrn.3844727","url":null,"abstract":"Crystallization experiments performed with highly supercooled solutions produced highly pure (> 99 wt%) and highly crystalline mesocrystals of curcumin from impure solution (~22% of two structurally similar impurities) in one step. These mesocrystals exhibited a crystallographic hierarchy and were composed of perfectly or imperfectly aligned nanometer thick crystallites. X-ray diffraction and spectroscopic analysis confirmed that the spherulites are a new solid form of curcumin. A theoretical hypothesis based on particle aggregation, double nucleation and a repeated secondary nucleation is proposed to explain the spherulite formation mechanism. The experimental results provide for the first time evidence for an organic molecule to naturally form spherulites without the presence of any stabilizing agent. Control experiments performed with highly supercooled pure solutions produced spherulites confirming the formation of spherulites is attributed to the high degree of supercooling and not due to the presence of impurities. Likewise control experiments performed with a lower degree of supercooling produced impure crystals of curcumin via the classical molecular addition mechanisms. These experimental observations all together provide first time evidence for particle mediated crystallization as an alternate and efficient method to purify organic compounds.","PeriodicalId":286484,"journal":{"name":"BiochemRN: Other Biochemistry Methods (Topic)","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129621335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0