P. Borden, Peng Zhang, Amol V. Shivange, J. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, O. Novák, M. Tanimoto, E. Shigetomi, Mark A. Lobas, Hyun-Tae Kim, Paula K Zhu, Yajun Zhang, W. Zheng, Chengcheng Fan, Guangfu Wang, Bowen Xiang, Lihua Gan, Guang-Xian Zhang, Kaiming Guo, Li Lin, Yuan Cai, Andrew G Yee, Abhi Aggarwal, Huan Bao, Xiaochu Lou, E. Chapman, C. P. Ford, D. Rees, D. Dietrich, B. Khakh, J. Dittman, W. Gan, Minoru Koyama, V. Jayaraman, J. Cheer, H. Lester, J. J. Zhu, L. Looger
{"title":"A Fast Genetically Encoded Fluorescent Sensor for Faithful in vivo Acetylcholine Detection in Mice, Fish, Worms and Flies","authors":"P. Borden, Peng Zhang, Amol V. Shivange, J. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, O. Novák, M. Tanimoto, E. Shigetomi, Mark A. Lobas, Hyun-Tae Kim, Paula K Zhu, Yajun Zhang, W. Zheng, Chengcheng Fan, Guangfu Wang, Bowen Xiang, Lihua Gan, Guang-Xian Zhang, Kaiming Guo, Li Lin, Yuan Cai, Andrew G Yee, Abhi Aggarwal, Huan Bao, Xiaochu Lou, E. Chapman, C. P. Ford, D. Rees, D. Dietrich, B. Khakh, J. Dittman, W. Gan, Minoru Koyama, V. Jayaraman, J. Cheer, H. Lester, J. J. Zhu, L. Looger","doi":"10.2139/ssrn.3554080","DOIUrl":null,"url":null,"abstract":"Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all organisms.","PeriodicalId":286484,"journal":{"name":"BiochemRN: Other Biochemistry Methods (Topic)","volume":"154 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BiochemRN: Other Biochemistry Methods (Topic)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2139/ssrn.3554080","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all organisms.
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