Hittite Journal of Science and Engineering最新文献_第2页

Nucleobase-modified microgels synthesized via microfabrication technology for DNA adsorption 通过微加工技术合成的用于 DNA 吸附的核碱基修饰微凝胶

Hittite Journal of Science and Engineering Pub Date : 2023-10-12 DOI: 10.17350/hjse19030000320

Kemal Çetin

{"title":"Nucleobase-modified microgels synthesized via microfabrication technology for DNA adsorption","authors":"Kemal Çetin","doi":"10.17350/hjse19030000320","DOIUrl":"https://doi.org/10.17350/hjse19030000320","url":null,"abstract":"DNA isolation is a crucial procedure since DNA-based assays have great importance in molecular biology, biochemistry and biomedical applications. The objective of this study is to fabricate micron-sized hydrogels as adsorbents for DNA. Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) microgels were synthesized by free radical polymerization in the presence of N,N'-methylenebisacrylamide as a crosslinker, in the microholes of a microstencil array chip. Then, adenine was immobilized to microgels through the epoxy groups of glycidyl methacrylate. Scanning electron microscopy and Fourier transform infrared spectroscopy were employed to investigate the chemical and morphological characterizations of the microgels. Adsorption studies were carried out to determine the optimal conditions for DNA adsorption of nucleobase-immobilized microgels. After initially increasing, the quantity of DNA adsorbed onto the microgels reached a saturation level at a DNA concentration of around 2.0 mg/mL. The maximum adsorption was 38.54 mg/g microgels for an initial DNA concentration of 2.0 mg/mL in the optimum medium pH and temperature. DNA adsorption capabilities are shown to not significantly decline in recurrent adsorption-desorption cycles. As a result of the findings, adenine-immobilized microgels were demonstrated to be a viable option for DNA adsorption.","PeriodicalId":285705,"journal":{"name":"Hittite Journal of Science and Engineering","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139320101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Camera Resolution on the Determination of Mechanical and Multiple Crack Properties of Engineered Cementitious Composites via Digital Image Correlation 相机分辨率对数字图像相关法测定工程胶凝复合材料力学和多重裂纹性能的影响

Hittite Journal of Science and Engineering Pub Date : 2023-06-30 DOI: 10.17350/hjse19030000298

Eren Gödek, Kamile TOSUN FELEKOĞLU

{"title":"Effect of Camera Resolution on the Determination of Mechanical and Multiple Crack Properties of Engineered Cementitious Composites via Digital Image Correlation","authors":"Eren Gödek, Kamile TOSUN FELEKOĞLU","doi":"10.17350/hjse19030000298","DOIUrl":"https://doi.org/10.17350/hjse19030000298","url":null,"abstract":"In this study, the effect of image resolution on the determination of mechanical parameters and multiple crack properties of Engineered Cementitious Composites (ECC) was investigated by digital image correlation (DIC). For this purpose, low and high resolution images were captured during the tensile loading of ECC by using two cameras simultaneously. DIC analyzes were performed on the images and results were compared both within themselves and with the traditional method. Mechanical parameters have been successfully calculated with DIC analysis and consistent values obtained with traditional methodology. However, high resolution images were found to be more effective for detecting pattern properties than low resolution images. It resulted in low strain error when using high resolution images compared to low resolution images in DIC analysis. Finally, when the high resolution images were used in DIC analysis as opposed to the low resolution images, both the detected number of cracks and calculated crack widths were obtained accurately. This situation was proven by local strain maps provided by DIC.","PeriodicalId":285705,"journal":{"name":"Hittite Journal of Science and Engineering","volume":"123 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121398686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Determination and Speciation of Selenium in Pharmaceutical Samples, Spiked Veterinary Drug Samples with a Kinetic Catalytic Method 动力学催化法测定兽药样品中硒的含量及形态

Hittite Journal of Science and Engineering Pub Date : 2023-06-30 DOI: 10.17350/hjse19030000296

E. Bağda

引用次数: 0

Synthesis of Pyridine-Based Imine Compounds and Molecular Docking Studies Against Dopamine D2 Receptors 吡啶基亚胺化合物的合成及其与多巴胺D2受体的分子对接研究

Hittite Journal of Science and Engineering Pub Date : 2023-06-30 DOI: 10.17350/hjse19030000303

S. Aytaç

引用次数: 0

Molecular Docking Studies and ADME Predictions on Synthesized Chalcone Compounds Targeting EGFR 靶向EGFR的合成查尔酮化合物的分子对接研究及ADME预测

Hittite Journal of Science and Engineering Pub Date : 2023-06-30 DOI: 10.17350/hjse19030000304

Ö. Gündoğdu

引用次数: 0

Physicochemical Analysis of Pomegranate Sours Produced by Traditional Method in Türkiye and The Investigation of Antioxidant Properties 传统法制备石榴酸的理化分析及抗氧化性能研究

Hittite Journal of Science and Engineering Pub Date : 2023-06-30 DOI: 10.17350/hjse19030000299

Miraç Oğuz, B. Akar, Cemallettin Baltaci

{"title":"Physicochemical Analysis of Pomegranate Sours Produced by Traditional Method in Türkiye and The Investigation of Antioxidant Properties","authors":"Miraç Oğuz, B. Akar, Cemallettin Baltaci","doi":"10.17350/hjse19030000299","DOIUrl":"https://doi.org/10.17350/hjse19030000299","url":null,"abstract":"In this study, the physicochemical properties and antioxidant activities of the commercially produced 15 pomegranate sour samples and one control sample were determined and their compliance with the TS 12720 (traditional sour pomegranate concentrate standard) was evaluated. Antioxidant activity values of pomegranate sours extracts were determined using 6 different methods. The samples had strong antioxidant capacity, except for N10 and N15. In addition, glucose, fructose, sucrose, HMF and acidity measurements of the same samples did not show compatibility with TS 12720. The pH values of the samples of N7, N8, N10, N11, N14 and N15 were not in accordance with the standard values (2.4-4.0) The titratable acidity values measured in samples of N1, N3, N4, N6, N10, N13, N15 were below the value (>6.0% (m/m)) in the standard. While the brix values of the samples were measured between 59.20-75.70, the brix values of the N12, N13 and N14 samples were determined below the standard brix value (>68%). The highest HMF value of the samples were determined as 8117.66. According to TSE 12720, the HMF content should be not exceed 50 mg/kg. However, the HMF values of the samples were detected above the maximum limit value except for N8 and N16.","PeriodicalId":285705,"journal":{"name":"Hittite Journal of Science and Engineering","volume":"555 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127077118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperthermia Efficacy of PEGylated-PLGA Coated Monodisperse Iron Oxide Nanoparticles 聚乙二醇-聚乳酸包覆单分散氧化铁纳米颗粒的热疗效果

Hittite Journal of Science and Engineering Pub Date : 2023-06-30 DOI: 10.17350/hjse19030000302

F. Senturk

{"title":"Hyperthermia Efficacy of PEGylated-PLGA Coated Monodisperse Iron Oxide Nanoparticles","authors":"F. Senturk","doi":"10.17350/hjse19030000302","DOIUrl":"https://doi.org/10.17350/hjse19030000302","url":null,"abstract":"Magnetic nano hyperthermia (MNH) is a promising technique for the treatment of a variety of malignancies. This non-invasive technique employs magnetic nanoparticles and alternating magnetic fields to generate local heat at the tumor location, which activates cell death pathways. However, the efficacy of MNH is dependent on the physicochemical properties of the magnetic nanoparticles, such as size, size distribution, magnetic properties, biocompatibility, and dispersibility in the medium. In this study, it is aimed to evaluate the heating capacity of poly (lactic-co-glycolic acid)-poly (ethylene glycol) di-block copolymer (PLGA-b-PEG) coated monodisperse iron oxide nanoparticles (IONs) as an effective mediator for MNH application. For this purpose, monodisperse IONs with a narrow size distribution and a mean particle size of 8.6 nm have been synthesized via the thermal decomposition method. The resulting IONs were then coated with the PEGylated-PLGA polymer and homogeneously dispersed in the polymeric matrix, which had a clearly defined spherical shape. Additionally, the specific absorption rate (SAR), reflecting the amount of heat dissipation from the NPs to the surrounding medium, was calculated for different concentrations (10, 5, 2.5, and 1.25 mg/mL) of PEGylated-PLGA-IONs. At 5 mg/mL PEGylated-PLGA-IONs (125 μgFe/mL) were found to have a maximum SAR value of 313 W/g. In conclusion, the homogenous dispersion of IONs in PEGylated-PLGA matrix may be one of the critical parameters to enhance the SAR value for MNH-based cancer therapy.","PeriodicalId":285705,"journal":{"name":"Hittite Journal of Science and Engineering","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128029876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adsorption and Kinetic Studies of Methylene Blue from Aqueous Solution Using Modified Spent Tea Leaves 改性茶叶对水溶液中亚甲基蓝的吸附及动力学研究

Hittite Journal of Science and Engineering Pub Date : 2023-06-30 DOI: 10.17350/hjse19030000301

Mine Nur Özyaman, Sena Çağşirli, Yeliz Özüdoğru

引用次数: 0

Novel 1,3,4-Thiadiazole Derivatives as Antibiofilm, Antimicrobial, Efflux Pump Inhibiting Agents and Their ADMET Characterizations 新型1,3,4-噻二唑类抗生物膜、抗菌、外排泵抑制剂及其ADMET表征

Hittite Journal of Science and Engineering Pub Date : 2023-06-30 DOI: 10.17350/hjse19030000297

M. Gür, Merve Zurnacı, E. Altınöz, Nesrin Şener, Ç. Şahi̇n, Merve Şenturan, İ. Şener, M. Çavuş, Ergin Murat Altuner

{"title":"Novel 1,3,4-Thiadiazole Derivatives as Antibiofilm, Antimicrobial, Efflux Pump Inhibiting Agents and Their ADMET Characterizations","authors":"M. Gür, Merve Zurnacı, E. Altınöz, Nesrin Şener, Ç. Şahi̇n, Merve Şenturan, İ. Şener, M. Çavuş, Ergin Murat Altuner","doi":"10.17350/hjse19030000297","DOIUrl":"https://doi.org/10.17350/hjse19030000297","url":null,"abstract":"In this study, 1,3,4-thiadiazole derivatives were obtained from the reaction of benzophenone-4,4'-dicarboxylic acid and N-substitute-thiosemicarbazide compounds with each other. After the synthesis of the final products, some biological properties of these compounds such as antibiofilm, antimicrobial and efflux pump inhibiting efficiencies were evaluated. According to the MBC/MFC test, all the activities were found to be bacteriostatic, also, especially the biofilm inhibition activity of C1 against K. pneumoniae is noteworthy. In addition, C4 was observed to exhibit efflux pump inhibition activity in E. coli, whereas C2 and C3 in K. pneumoniae. The absorption and emission values of the molecules were obtained and electrochemical studies were performed. In addition; absorption, metabolism, distribution, excretion and toxicity (ADMET) scores were predicted using the pharmacokinetic properties of all 1,3,4-thiadiazole compounds. Finally, the electrochemical stabilities of the synthesized molecules have been analyzed by using cyclic voltammetry in 0.1 M TBAPF6 in DMSO as a supporting electrolyte.","PeriodicalId":285705,"journal":{"name":"Hittite Journal of Science and Engineering","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115783871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Performance Comparison of SC-PPM Receiver Models SC-PPM接收机型号的性能比较

Hittite Journal of Science and Engineering Pub Date : 2023-06-30 DOI: 10.17350/hjse19030000306

Mehmet Sonmez

{"title":"The Performance Comparison of SC-PPM Receiver Models","authors":"Mehmet Sonmez","doi":"10.17350/hjse19030000306","DOIUrl":"https://doi.org/10.17350/hjse19030000306","url":null,"abstract":"Visible light communication (VLC) technology has arisen as promising candidate to solve the critical challenges of wireless communication networks. In particular, the evolving explosion in use of internet services requiring high bandwidth will soon become a great potential problem among the service provider. Meanwhile, this paper describes a physical layer solution to provide data transmission in the VLC networks. In the paper, it is aim to examine the performance comparison of SC-PPM (Subcarrier Pulse Position Modulation) demodulator schemes. It is considered three receiver techniques to assemble the demodulator techniques to SC-PPM receiver system. Firstly, the traditional PPM (T-PPM) demodulator has been applied on the SC-4PPM receiver system to estimate the slot that includes high frequency signal that is referred to as subcarrier signal. To successfully detect the bits by using traditional PPM receiver, it must be known the dimming level of received SC-4PPM signal. This is a serious problem to ensure data transmission in the real-time VLC systems due to challenge of providing the variable dimming level knowledge at the receiver side. In another receiver model that is referred to as PD (Peak Detector), it is aim to detect peak values of slot with high frequency signal. The disadvantage of this system is that BER (Bit Error Rate) performance depends the difference between peak and bottom values of subcarrier filled slot. Hence, second method is improved to achieve the similar BER performance at all dimming levels between 12.5% and 87.5%. This receiver model is called as IPD in the paper. In brief, it is reported for the first time, it has been employed the PD and the IPD algorithms for the SC-PPM receiver\u0000schemes. In addition to this, it is given a theoretical framework for both the traditional PPM and the improved receiver schemes in VLC-SC-PPM schemes. Moreover, it has been investigated how the SC-PPM receiver schemes are affected by brightness level.","PeriodicalId":285705,"journal":{"name":"Hittite Journal of Science and Engineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130976086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0