Growing and Handling of Bacterial Cultures最新文献

Carbapenemases "

Growing and Handling of Bacterial Cultures Pub Date : 2019-12-04 DOI: 10.5772/intechopen.76282

B. Bedenić, S. Sardelić

引用次数: 5

The War between Bacteria and Bacteriophages 细菌和噬菌体之间的战争

Growing and Handling of Bacterial Cultures Pub Date : 2019-10-31 DOI: 10.5772/intechopen.87247

B. Orzechowska, M. Mohammed

{"title":"The War between Bacteria and Bacteriophages","authors":"B. Orzechowska, M. Mohammed","doi":"10.5772/intechopen.87247","DOIUrl":"https://doi.org/10.5772/intechopen.87247","url":null,"abstract":"The rapid emergence and dissemination of multidrug-resistant (MDR) bacteria represents a worldwide crisis concerning that humankind is re-entering the ‘pre-antibiotics’ era. Before the discovery of antibiotics, bacteriophage therapy was widely enforced to combat bacterial infections. However, the discovery of penicillin in 1940 and other novel antibiotics replaced phage therapy, and they are being used as the first line of defence against pathogenic bacterial infections. Factors such as selective pressure resulted in bacteria becoming insensitive to one or multiple antibiotics, frequently leading to limited treatment options. This prompted a renewal of interest to the phage therapy that remains dubious due to its disadvantages such as host specificity and the development of bacterial resistance against phages. Evolution of bacterial genomes allowed bacteria to acquire vast mechanisms interfering with phage infection such as inhibition of phage adsorption, prevention of phage entry, superinfection exclusion, restriction-modification and abortive infection. Interestingly, phages have developed diverse counterstrategies to circumvent bacterial anti-phage mechanisms including digging for receptors, adapting to new receptors and masking and modifying restriction sites. Understanding the complex dynamics of bacteria-phage interaction is a preliminary step towards designing synthetic phages that can overcome limitations of phage therapy and potentially lead to defeating MDR bacteria.","PeriodicalId":282689,"journal":{"name":"Growing and Handling of Bacterial Cultures","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124723904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Design and Operation of Fixed-Bed Bioreactors for Immobilized Bacterial Culture 固定化细菌培养固定床生物反应器的设计与运行

Growing and Handling of Bacterial Cultures Pub Date : 2019-10-09 DOI: 10.5772/intechopen.87944

R. Pörtner, Rebecca Faschian

引用次数: 4

Mycelium Differentiation and Development ofStreptomycesin Liquid Nonsporulating Cultures: Programmed Cell Death, Differentiation, and Lysis Condition Secondary Metabolite Production 链霉菌素液体非孢子培养菌丝的分化和发育:程序性细胞死亡、分化和裂解条件次生代谢物的产生

Growing and Handling of Bacterial Cultures Pub Date : 2019-05-03 DOI: 10.5772/INTECHOPEN.84182

Á. Manteca, B. Rioseras, Nathaly González-Quiñónez, Gemma Fernández-García, P. Yagüe

{"title":"Mycelium Differentiation and Development ofStreptomycesin Liquid Nonsporulating Cultures: Programmed Cell Death, Differentiation, and Lysis Condition Secondary Metabolite Production","authors":"Á. Manteca, B. Rioseras, Nathaly González-Quiñónez, Gemma Fernández-García, P. Yagüe","doi":"10.5772/INTECHOPEN.84182","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.84182","url":null,"abstract":"Streptomycetes are mycelium-forming sporulating bacteria that produce two thirds of clinically relevant secondary metabolites. Secondary metabolite production is activated at specific developmental stages of Streptomyces life cycle. Despite this, Streptomyces differentiation in liquid nonsporulating cultures (flasks and industrial bioreactors) tends to be underestimated and the most important parameters managed are only indirectly related to differentiation: modifications to the culture media, optimization of productive strains by random or directed mutagenesis, analysis of biophysical parameters, etc. In this chapter, we review the relationship between differentiation and antibiotic production in liquid cultures. Morphological differentiation in liquid cultures is comparable to that occurring during pre-sporulation stages in solid cultures: an initial compartmentalized mycelium suffers a programmed cell death, and remaining viable segments then differentiate to a second multinucleated antibiotic-producing mycelium. Differentiation is one of the keys to interpreting biophysical fermentation parameters and to rationalizing the optimization of secondary metabolite production in liquid cultures.","PeriodicalId":282689,"journal":{"name":"Growing and Handling of Bacterial Cultures","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129007203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

What Is Limulus Amebocyte Lysate (LAL) and Its Applicability in Endotoxin Quantification of Pharma Products 什么是鲎试剂(LAL)及其在药品内毒素定量中的应用

Growing and Handling of Bacterial Cultures Pub Date : 2019-01-08 DOI: 10.5772/INTECHOPEN.81331

Y. Mehmood

引用次数: 5

Growing and Handling of Bacterial Cultures within a Shared Core Facility for Integrated Structural Biology Program 综合结构生物学项目共享核心设施内细菌培养物的生长和处理

Growing and Handling of Bacterial Cultures Pub Date : 2018-11-08 DOI: 10.5772/INTECHOPEN.81932

L. Warner, O. Mass, N. D. Lenn, Briana R. Grantham, J. Oxford

引用次数: 3

GeobacillusBacteria: Potential Commercial Applications in Industry, Bioremediation, and Bioenergy Production 地杆菌:在工业、生物修复和生物能源生产中的潜在商业应用

Growing and Handling of Bacterial Cultures Pub Date : 2018-11-05 DOI: 10.5772/INTECHOPEN.76053

G. Novik, V. Savich, Olga Meerovskaya

{"title":"GeobacillusBacteria: Potential Commercial Applications in Industry, Bioremediation, and Bioenergy Production","authors":"G. Novik, V. Savich, Olga Meerovskaya","doi":"10.5772/INTECHOPEN.76053","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.76053","url":null,"abstract":"The genus Geobacillus is represented by obligately thermophilic bacteria able to grow in the temperature range of 35–75°C. They are modest bacteria isolated from various sources on routine media such as nutrient agar. Originally classified as representatives of Bacillus , the species of Geobacillus were established in 2001 as a new genus. However, sequence similarity between all species indicates that at least some species need to be reclassified at the genus level. In addition to 16S rRNA, housekeeping genes, 16S-23S rRNA gene internal transcribed spacer, and repetitive sequences can be used in classification and identification of thermophilic bacteria. The ability to survive and grow at high temperatures as well as utilization and synthesis of a wide range of compounds makes these bacteria and their products attractive for use in various spheres: food, paper, biotechnology industries, medicine, bioremediation, etc. A broad spectrum of applications arouses increased interest in the study of physiological and biochemical characteristics and triggers emergence of new usage areas for Geobacillus , such as bioenergy. The growing demand for energy leads to the development of alternative technologic options. Geobacillus species demonstrated the ability to generate or enhance productivity of important sources of bioenergy such as ethanol, isobutanol, 2,3-butanediol, biodiesel, and biogas.","PeriodicalId":282689,"journal":{"name":"Growing and Handling of Bacterial Cultures","volume":"142 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125766751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13