The journal of Kansai Medical University最新文献

{"title":"Medical Education in Berlin-Reformed Curriculum and Communication Skills Training","authors":"H. Ortwein, I. Muehlinghaus, Schnabel Kp, P. Terzioglu, A. Wilke, D. Scheffner, W. Burger","doi":"10.5361/JKMU1956.56.2-4_172","DOIUrl":"https://doi.org/10.5361/JKMU1956.56.2-4_172","url":null,"abstract":"","PeriodicalId":281939,"journal":{"name":"The journal of Kansai Medical University","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129460467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"急性および慢性ラット肝障害の肝星細胞におけるTGF-β シグナル伝達機構に関する検討","authors":"腎也 田橋","doi":"10.5361/JKMU1956.55.2-4_167","DOIUrl":"https://doi.org/10.5361/JKMU1956.55.2-4_167","url":null,"abstract":"","PeriodicalId":281939,"journal":{"name":"The journal of Kansai Medical University","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2003-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132257263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dosage Dependent Suppression of a crp - Mutant by yciM Gene Product in Escherichia coli","authors":"S. Mizutani, A. Umemoto, F. Fattah, K. Fattah, Toshimasa Nishiyama, Y. Sugino","doi":"10.5361/JKMU1956.54.2-4_119","DOIUrl":"https://doi.org/10.5361/JKMU1956.54.2-4_119","url":null,"abstract":"","PeriodicalId":281939,"journal":{"name":"The journal of Kansai Medical University","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124933396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ラムドイド・ファージ・ゲノムの比較","authors":"義信 杉野","doi":"10.5361/JKMU1956.54.2-4_164","DOIUrl":"https://doi.org/10.5361/JKMU1956.54.2-4_164","url":null,"abstract":"","PeriodicalId":281939,"journal":{"name":"The journal of Kansai Medical University","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132846805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Our Experience of Muscle Afferent Block for dystonia and spasticity","authors":"Hisashi Ito, S. Nakano, Hidefumi Ito, H. Kusaka","doi":"10.5361/JKMU1956.54.2-4_130","DOIUrl":"https://doi.org/10.5361/JKMU1956.54.2-4_130","url":null,"abstract":"Muscle Afferent Block (MAB) has been shown to improve clinical symptoms of writer's cramp, cervical dystonia, spastic paraparesis, and oromandibular dystonia. However, this therapy is not yet used to a sufficient extent. In this article, we review our experience with this new treatment The subjects were 11 cases encountered between November, 2001 and April, 2002, including 4 cases of focal dystonia of an upper limb, 3 cases of spastic paralysis of an upper limb, 3 cases of spastic paraparesis, and 1 case of spasmodic torticollis. All patients exhibited some clinical improvement. Two cases of facial flushing and 3 cases of muscle weakness occurred as side effects. All of them were mild and transient. We conclude that MAB is a useful treatment for dystonia and spasticity with low incidence of side effects. Introduction In 1924, Walshe reported that intramuscular injection of diluted procaine alleviated rigidity in a patient with Parkinsonismi). This report had been ignored for years, but on the basis of this report, Kaji et al. reported the efficacy of 0.5% xylocaine and 99% ethyl alcohol for writer's cramp in 19952). This therapy is currently applied to upper limb dystonia including writer's cramp 2 ) 3 , spasmodic torticollis , spastic paraparesis 5 , upper limb spasticity 5 ) 6), and oromandibular dystonia 7 , and has been reported to be likely to show invariable efficacy. We performed MAB in 11 patients at our department from November 2001 to April 2002, and confirmed that symptoms were alleviated just after the therapy (shortterm efficacy) in all patients and that alleviation of the symptoms lasted for at least 1 week in 9 patients as of April 2002. In this report, we explain the technique of MAB and report the therapeutic results obtained at our department. Principle of MAB and Its Technique in Practice Walshe thought that blockade of sensory afferent fibers by local anesthetics led to hypotonia ), but this hypothesis has been denied on the basis of electrophysiological findings 3 . Local anesthetics at a low concentration are known to block short-diameter fibers selectively8), and it has been hypothesized that in MAB, xylocaine at a low concentration blocks y -afferent small fibers, regulating the sensitivity of muscle spindles, resulting hypotonia9). However, this hypothesis has not been proved, and the principle of MAB is poorly understood. Kaji et a!. did not only selected xylocaine, a safer drug than procaine, but also reduced the total dose of 0.5% xylocaine to be used in one occasion of the therapy to 50 ml on the basis of the safety margin. In addition, they used ethyl alcohol, which is known to block Na+ channels in nerve fibersiw like other local anesthetics, at the dose equivalent to 10% of the total dose of xylocaine, to maintain the efficacy. One syringe containing 10 ml of 0.5% xylocaine and the other containing 99% ethyl alcohol are connected to a three-direction stopper which is connected to an injection needle through an exte","PeriodicalId":281939,"journal":{"name":"The journal of Kansai Medical University","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134480471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"powerful new strategy for allogeneic bone marrow transplantation in mice","authors":"T. Kushida, M. Inaba, H. Hisha, Naoya Ichioka, Takashi Esumi, R. Ogawa, H. Iida, S. Ikehara","doi":"10.5361/JKMU1956.54.2-4_136","DOIUrl":"https://doi.org/10.5361/JKMU1956.54.2-4_136","url":null,"abstract":"We have succeeded in treating intractable autoimmune diseases in chimeric resistant MRL/lpr mice by a new bone marrow transplantation (BMT) method consisting of fractionated irradiation (5.5 Gy x2) followed by intra-bone marrow (IBM) injection of whole bone marrow cells (BMCs) from allogeneic normal C 57 BL/6 (B 6) mice [5.5 Gy X2+ IBM]. In MRL/Ipr mice treated with this method, the number of not only donor-derived cells but also donor-derived hemopoietic progenitor cells increased. Furthermore, donor-derived stromal cells were clearly detected in the cultured bone pieces from MRL/lpr mice treated with [5.5 GyX2+IBM]. All the recipients thus treated survived more than one year ( >60 weeks after birth) and remained free from autoimmune diseases. Autoantibodies decreased to almost normal levels, and abnormal T cells (Thy 1.2+/B 220k+CD 4-/CD 8-) disappeared. These findings clearly indicate that this new strategy ( \"IBM-BMT\") creates the appropriate hemopoietic environment for the early recovery of hemopoiesis and donor cell engraftment, resulting in the complete amelioration of intractable autoimmune diseases in chimeric resistant MRL/lpr mice without recourse to immunosuppressants. This strategy would therefore be applicable to human therapy. Introduction We have previously shown using various autoimmuneprone mice that conventional allo BMT can be used to prevent and treat a range of autoimmune diseases. 1-3 These findings have recently been confirmed even in humans.46 However, in humans, the success rate of BMT across major histocompatibility complex (MHC) barriers is lowered by graft-versus-host disease (GvHD), graft rejection, and incomplete T-cell recovery. Therefore, autologous BMT (auto BMT) or auto peripheral blood stem cell transplantation (PBSCT) is the preferred treatment for autoimmune diseases. There have, however, been reports on the rapid recurrence or persistence of autoimmune diseases after auto BMT or auto PBSCT7 Therefore, it is important to establish a safe new method for allo BMT. 8 We have found that the MRL/lpr mouse, an animal model for autoimmune diseases, is a suitable model for establishing a safe new strategy for all BMT, since the MRL/lpr mouse itself is radio-sensitive (8.5 Gy), while the abnormal hemopoietic stem cells of the MRL/lpr mouse are radio-resistant ( >8.5 Gy); conventional BMT (8.5 Gy plus allo BMT) has a transient effect on autoimmune diseases, which recur 3 months after the BMT. 8 To prevent the recurrence of autoimmune diseases in MRL/lpr mice, we carried out BMT plus bone grafts to replace not only the hemopoietic cells but also the stromal cells with donor cells. This is because there is a MHC restriction between pluripotent hemopoietic stem cells (P-HSCs) and stromal cells. ,11) MRUIpr mice that had been irradiated (8.5 Gy) and then reconstituted with C 57 BL/6 BMCs plus bone grafts survived more than 1","PeriodicalId":281939,"journal":{"name":"The journal of Kansai Medical University","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129510525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in cortical field potentials in learning processes of audio-initiated vocalization and influences of cerebellectomy upon the vocalization in the monkey","authors":"H. Gemba, S. Kyuhou, R. Matsuzaki, Yae Amino, Kazuko Matsuura, Tomomi Seki, R. Shibata","doi":"10.5361/JKMU1956.54.2-4_124","DOIUrl":"https://doi.org/10.5361/JKMU1956.54.2-4_124","url":null,"abstract":"Recording field potentials during learning processes of audio-initiated vocalization by electrodes implanted chronically on the surface and at 2.0-3.0 mm depth of the cerebral cortex and analyzing them seem useful for knowing central nervous mechanisms of the vocalization in the monkey. We have been waiting for an opportunity to record the potentials during the motor learning in a short period, because it usually takes a considerable time (3-6 months) for a naive monkey to be trained well for the vocalization. One of three monkeys, which had been fully trained for the vocalization, was found to be unable to perform the vocalization after electrode-implanting operation. We could record the potentials in the monkey during the process of learning the vocalization again. The learning process of the vocalization partly differed from audio-initiated hand movements previously reported, and there was no skill learning in the vocalization. The right cerebellar hemispherectomy eliminated the surface-negative, depth-positive (s-N, d-P) premovement potential recorded before the operation in the left face motor cortices in the three monkeys, and changed vocal tone. Scarce change was seen in reaction times in audio-initiated vocalizations before and after the operation, again differing from audio-initiated hand movements. This suggests that central nervous mechanisms in audio-initiated vocalizations differ from those in audio-initiated hand movements. These nervous mechanisms were discussed in connection with the limbic system. Introduction We thought that studies on cortical involvement in monkey vocal communication would be helpful for clarifying the brain mechanism in human speech. Monkeys were trained to vocalize in response to auditory stimulus (monkey's coo call) (audio-initiated vocalization), and cortical field potentials associated with the vocalization were studied by the above-mentioned electrodes. An upward (s-N, d-P) potential (at about 70 ms latency after stimulus onset) was recorded in the rostral bank of the inferior limb of the arcuate sulcus in the left hemisphere, in which an insignificant potential was associated with self-paced vocalizations. An upward (s-N, d-P) slow potential in the motor and somatosensory cortices associated with audio-initiated vocalizations was substantially the same in duration and amplitude as the potential associated with self-paced vocalizations. Reaction times from stimulus onset to vocalization start were variable, but were about 0.9 s on the average (3). The findings suggested that there were some differences between central nervous mechanisms in audio-initiated vocalization and those for reaction-time hand movements previously reported (2, 4, 6, 7, 11). In order to investigate the differences in more detail, it seemed significant to study cortical field potentials during the learning processes of the audio-initiated vocalization, and influences of cerebellectomy upon the vocalization. In the present study, thr","PeriodicalId":281939,"journal":{"name":"The journal of Kansai Medical University","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126213643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Indicators for Optimal Dialysis Based on Creatinine Kinetics","authors":"Yasuhiro, Isami, Toshihiro, Ikuma, Mineo, Okamoto, Shunpei, Mizutani, Mikiko, Muramatsu, Satoko, Ono, Shousaku, Nomura, Mayumi, Kuzushita, Motoko, Masaki, Mika, Oomiya, Masahiro, Okuda, Yuka, Akashi, Yoshihiro, Tagawa, Hisato, Nakamori, Tetsusi, Wakai, Nobuyuki, Takahashi, Akira, Nakamura, Muneto, Yoshioka, Yuji, Naito, Eiichi, Tsuda","doi":"10.5361/JKMU1956.53.2-4_125","DOIUrl":"https://doi.org/10.5361/JKMU1956.53.2-4_125","url":null,"abstract":"Background; Creatinine (Cr) is supposed to be uniformly distributed in body fluid, as is the case with urea nitrogen (UN). We calculated the normalized dialyser serum Cr (S-Cr) clearnce (Kt/Vcr), time averaged concentration of S-Cr (TACcr), and creatinine generation rate per body weight (gCr) on the basis of creatinine kinetics instead of urea kinetics and assessed whether these parameters would provide useful new indicators for optimal dialysis. Methods; Blood UN (BUN) and S-Cr values were measured in 13 hemodialysis patients immediately before and after a session of dialysis and immediately before the next session. Based on these measurements, the new indicators were calculated and compared with the conventional indicators [normalized dialyser urea clearnce (Kt/Vurea), time averaged concentration of BUN (TACBUN) , protein catabolic rate (per) ]. Results; There was a significant positive correlation (r = 0.95, P< 0.001) between Kt/Vcr (1.05 ± 0.15) and Kt/Vurea, and between TACcr (9.9 ± 1.6) and TACBUN (r = 0.85, P < 0.001) . The gCr (27.9 ± 5.5mg/kg/day) also correlated with per. Conclusion; Kt/Vcr and TACcr can be new indicators for optimal dialysis. The gCr may serve as a comprehensive indicator of nutritional conditions in hemodialysis","PeriodicalId":281939,"journal":{"name":"The journal of Kansai Medical University","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132251808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The recognition sequences of N proteins of the toxin-converting lambdoid phages: a comparative approach.","authors":"K. Fattah, S. Mizutani, F. J. Fattah, Yoshinobu Suvino","doi":"10.5361/JKMU1956.53.2-4_148","DOIUrl":"https://doi.org/10.5361/JKMU1956.53.2-4_148","url":null,"abstract":"In the N antitermination system of bacteriophage lambda and related phages, the BoxB of the nut site interacts with the N protein to form an antitermination complex. Arginine-rich sequences in the N-proximal region of these proteins in phage lambda, P22 and P21 have been assigned as the recognition sequences of these phages. An arginine-rich motif very similar to that of P22 N protein has been found in the N protein of phage HK97 and the toxin-converting phages 933W and H-19B, which we suggest to be the recognition sequence of these N proteins because these three phages have a BoxB loop sequence identical with that of P22. We also found a slightly different, but similarly located arginine-rich sequence in phage VT2-Sa N protein, which we suggest is the recognition sequence of the N protein for this phage. This is consistent with the fact that its BoxB loop is different from the other three phages. Introduction Lambdoid phages seem to be constructed by a combinatorial arrangement or shuffling of a limited number of modules or cassettes, each of which consists of one or more active specific protein (s) and its (their) target base sequences (Campbell, 1988; Juhala et al. , 2000) . The N antitermination system is one of these modules. The immunity region is another. (In our preceding paper (Fattah et al. , 2000) we failed to notice that phage HK97 has an almost identical immunity region with phage lambda (Juhala et al., 2000) , but their similarity was so overwhelming that it seemed almost obvious that they should possess identical immunity specificity. We would like to take this opportunity to correct our oversight.) The N antitermination requires the N protein coded by lambda, and several host proteins called NusA , NusB etc. , and cis-acting sites called nut. Following transcription of the nut site, N and Nus proteins bind to the nut RNA and modify the transcription complex to a termination-resistant form. The nut site is a composite of at least two components; one is the BoxB hairpin structure that interacts with N. The other is BoxA, a nine-nucleotide sequence upstream of BoxB (Patterson et al. , 1994) . Naomi Franklin has shown (Franklin, 1984) that this system is conserved in the relative arrangement in the genome (genome form) , but not in the (DNA) sequences in the three phages, lambda, 21 and P22. She has also shown that the target base sequence specificities of the N proteins are also different among these three phages. Since then much more sequence information has been accumulated. Here we attempt to see if her conclusions can be extended to other lambdoid phages. We focus on the target site of N action (nut sites) and the N proteins themselves, especially the recognition sequences (Lazinski et al. , 1989) in them that are involved in the specific interaction with the nut sites. Fig. 1. The nut sites of lambdoid phages. The BoxB loop sequences are shown in boxes. The sequences for lambda (Franklin, 1984) , P22 (Franklin, 1984) , H-19B (Ne","PeriodicalId":281939,"journal":{"name":"The journal of Kansai Medical University","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115526663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Sequencing by Tn3 Insertion in Vitro: Comparison of the Shotgun and Ordered Methods","authors":"F. Fattah, M. Morita, S. Mizutani, K. Fattah, Y. Sugino","doi":"10.5361/JKMU1956.53.2-4_135","DOIUrl":"https://doi.org/10.5361/JKMU1956.53.2-4_135","url":null,"abstract":"We sequenced an E. coil 6.6 kb fragment by using in vitro transposition reaction of Tn3. Tn3 inserted randomly into the target plasmid and the ends of the transposon can be used as movable primer sites. Our selection system eliminates cointegrates and leaves only the simple insertions which makes it suitable for sequencing. We applied both the shotgun method and the ordered method in order to compare them. The shotgun method required more clones but produced the total sequence quickly by the use of a suitable software such as DNAMAN. Moreover, the redundancy of sequencing involved in shotgun method has the advantage of making the result more reliable. The ordered method requires information about the order and separation of transposon insertion sites in different clones in order to select the plasmids to be used for sequencing, but resulted in minimum redundancy. However, the amount of preliminary work required in the ordered method before actual sequencing far outweighs the benefit in the reduction in the number of clones necessary to be sequenced. The E. colt: sequence we obtained turned out to be a recombinant between the standard database sequence (AE000441) and another sequence published by Jensen's group (X00781) within the rph gene. In vitro transposition of a transposon can be used as a tool in the sequencing of a DNA that is too long to be sequenced in a single step from a single primer, when the transposon has little insertion site specificity; ends of the transposon can be used as \"movable primer sites\" inserted in different locations in the DNA. Several systems are now commercially available that are based on this principle. Maekawa et al.1 has described an in vitro transposition system for Tn3. We modified their system to be suitable as a tool for use in sequencing a large DNA fragment. The modification consists in the choice of a selection system which allows only direct simple insertions of Tn3 to be selected, whereas Maekawa et al.'s system allows not only simple insertions but also cointegrates to survive. Cointergrates contain two copies of the transposon, which preclude their use as a sequencing aid. Our system can be used either as a shotgun method, because Tn3 can insert at random in DNA; or alternatively a preliminary ordering step can be included using appropriate restriction enzymes. In this paper we used this system to determine the sequence of a 6.6 kb E. coil BatnHI DNA fragment derived from strain DOO crp-2, cloned into the plasmid vector pKF33 to give the plasmid pFJK1. We tested both the shotgun method and a sequencing procedure including a preliminary ordering step by using restriction enzymes, in order to compare their merits and demerits. We used delta Tn3 transposon4 that is derived from Tn3. Most of the transposase gene is deleted in delta Tn3 contained in plasmid pMM2004. A cell extract was prepared from E. coil strain Km1196 harboring plasmid pMM2404 that overproduces Tn3 transposase. Cointegrates cannot survi","PeriodicalId":281939,"journal":{"name":"The journal of Kansai Medical University","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124351271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}