BERITA BIOLOGIPub Date : 2020-12-17DOI: 10.14203/BERITABIOLOGI.V19I3A.3933
A. Putri, I. N. Sumerta
{"title":"SELECTIVE ISOLATION OF Dactylosporangium AND Micromonospora FROM THE SOIL OF KARST CAVE OF SIMEULUE ISLAND AND THEIR ANTIBACTERIAL POTENCY","authors":"A. Putri, I. N. Sumerta","doi":"10.14203/BERITABIOLOGI.V19I3A.3933","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V19I3A.3933","url":null,"abstract":"Karst is a unique ecosystem that consists of a thin soil layer on the carbonate rocks. It has a diverse microorganism, especially actinomycetes group, which might potentially produce beneficial secondary metabolites that remain unknown. In this study, we were interested in isolating Dactylosporangium and Micromonospora that have been potentially reported as antibiotic sources. We used two methods using the chemical germicide (pretreatment 1.5% phenol) compared with the SDS-YE (0.05% SDS pretreatment). Thirty-nine isolates actinomycetes were successfully isolated from two samples of the karst cave soils. Thirteen isolates were obtained by using the germicide chemical method and closely related to the genera of Dactylosporangium and Micromonospora. On the other hand, the SDS-YE grew twenty-six isolates which closely related to 11 genera of actinomycetes (Catenulispora, Nocardia, Rhodococcus, Ornithinimicrobium, Catellatospora, Dactylosporangium, Micromonospora, Streptacidiphilus, Streptomyces, Nonomuraea, and Streptosporangium). These results suggest that 1.5% of phenol pretreatment could preserve the genera of Dactylosporangium and Micromonospora, while the Streptomyces and other rare actinomycetes were killed. Among all isolates, only 7 showed the antibacterial activity on tested bacteria. Even though the antibacterial activity of those isolates was not high, the isolation of actinomycetes from a specific substrate is necessary to be conducted on exploring the richness of our natural resources. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123780374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"UJI TOKSISITAS ORAL REPEATED DOSE FILTRAT BUAH LUWINGAN (Ficus hispida L.f.) MENGGUNAKAN MODEL TIKUS (Rattus norvegicus Berkenhout, 1769) GALUR WISTAR","authors":"Laksmindra Fitria, Rosita Dwi Putri Suranto, Indira Diah Utami, Septy Azizah Puspitasari","doi":"10.14203/BERITABIOLOGI.V19I3A.3936","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V19I3A.3936","url":null,"abstract":"Hairy fig is a tropical medium-sized tree that produces abundant fruits throughout the year. In some Asian countries, the fruits are consumed as traditional medicine and food ingredient. Meanwhile in Indonesia there has not been much use. A series of oral toxicity tests must be conducted to study the possibility of toxic effects and the safety before further exploration. Oral single dose toxicity study of young and ripe hairy fig fruit filtrate has been carried out. Results demonstrated no observed adverse effect level (NOAEL) at a concentration of 100%. This study was aimed to continue the oral toxicity test with repeated dose following standard toxicity procedure by OECD Test Guideline No. 407 with some modifications. Parameters observed were mortality, sublethal effects consisted of physical conditions and behavior, body weight, core temperature, complete blood count, as well as liver, heart, and renal functions by measuring ALT, AST, and creatinine, respectively. Sampling points on day 0, 7, 14, 21, and 28. Results showed no observed adverse effect level (NOAEL) in both young and ripe fruits filtrate at a concentration of 100% however with a tendency to cause anemia and associate with renal dysfunction. Therefore, it is necessary to perform similar method of toxicity test but with lower concentration, also continue with further toxicity tests (subchronic and chronic periods). ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127588673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2020-12-17DOI: 10.14203/BERITABIOLOGI.V19I3A.3675
O. Hidayat
{"title":"CATATAN PERKEMBANGBIAKAN MELIPHAGA DADA-LURIK (Microptilotis reticulatus) DI PULAU TIMOR DAN INFORMASI TERHADAP PERDAGANGANNYA","authors":"O. Hidayat","doi":"10.14203/BERITABIOLOGI.V19I3A.3675","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V19I3A.3675","url":null,"abstract":"Streak-breated honeyeater (Microptilotis reticulatus) is one of the endemic honeyeater birds from Meliphagidae family. Biological information on this species is very limited and poorly known. Field observation was carried out on the active nest found in 2 October 2013. In addition, investigation on social media (facebook) was carried out to examine the exploitation on this species. The results shows that the cup-shaped nest is 5.6 cm in diameter and 4.9 cm in depth, made by wood fibre, dry grass, glued by cotton fibre from plants and spider web on small branch. Nowadays M. reticulatus is trapped and traded without any regulation from management authority. As many as 651 individuals has been trapped and sold between 2016 and 2019. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"141 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134032569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2020-12-17DOI: 10.14203/BERITABIOLOGI.V19I3A.3992
Y. M. Mambrasar, Y. Robiah, Z Nira Ariasari, Yayan Supriyanti, D. Rosalina, S. Sutikno, J. Jaenudin, W. Santoso, Dede Surya, M. Megawati, Taufik Mahendra, Agusdin Dharma Fefirenta, D. Arifiani
{"title":"JAVANESE ENDEMIC STROBILANTHES(ACANTHACEAE): TAXONOMY, DISTRIBUTION AND CONSERVATION STATUS","authors":"Y. M. Mambrasar, Y. Robiah, Z Nira Ariasari, Yayan Supriyanti, D. Rosalina, S. Sutikno, J. Jaenudin, W. Santoso, Dede Surya, M. Megawati, Taufik Mahendra, Agusdin Dharma Fefirenta, D. Arifiani","doi":"10.14203/BERITABIOLOGI.V19I3A.3992","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V19I3A.3992","url":null,"abstract":"The taxonomic status of Javanese Strobilanthes has been revised by Bennett and Scotland (2003). However, the results of the validation of the Javanese Acanthaceae by Girmansyah (2014) show that several Strobilanthes species, especially those endemic to Java, need to be revised. Because they have become synonymous. Conservation status of Javanese endemic Strobilanthes was carried out according to the IUCN Red list Categories and Criteria. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117280083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2020-12-17DOI: 10.14203/BERITABIOLOGI.V19I3A.3868
A. Abdullah, R. Rahmawati, R. Kurniatuhadi
{"title":"ANTIFUNGAL EXTRACT ACTIVITYOF ISOLATE Nocardia sp. ATS-4.1 AGAINST Candida albicans InaCC-Y116","authors":"A. Abdullah, R. Rahmawati, R. Kurniatuhadi","doi":"10.14203/BERITABIOLOGI.V19I3A.3868","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V19I3A.3868","url":null,"abstract":"Candida albicans is a microorganism that knows as caused of candidiasis. Nocardia is known to have the ability to produce antifungal bioactive compounds to overcome cases of fungal infections. This research aims to determine the presence of antifungal activity and the good concentration from crude extract of Nocardia sp. ATS-4.1 to inhibit C. albicans InaCC-Y116 and. Antifungal activity test of isolate Nocardia sp. ATS-4.1 was performed using a well diffusion method on Mueller Hinton Agar (MHA) media with a concentration of 92%, 94%, 96%, 98%, 100% and nystatin (positive control) 0,0125% and DMSO 10% (negative control) incubated at 37 ᵒC for 24-48 hours. The results showed that the extract of Nocardia sp. ATS-4.1 can inhibit the growth of C. albicans. Nocardia sp. ATS-4.1 isolate extract concentration of 96% with a resistance diameter of 13.63 ± 0.53 mm with a strong category against the growth of C. albicans InaCC-Y116. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115408678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2020-12-17DOI: 10.14203/BERITABIOLOGI.V19I3A.3890
N. T. Pratiwi, Qadar Hasani, Ahmad Muhtadi, N. Kautsari
{"title":"PERTUMBUHAN DAN PRODUKTIVITAS Oedogonium sp. PADA INTENSITAS CAHAYA YANG BERBEDA","authors":"N. T. Pratiwi, Qadar Hasani, Ahmad Muhtadi, N. Kautsari","doi":"10.14203/BERITABIOLOGI.V19I3A.3890","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V19I3A.3890","url":null,"abstract":"Oedogonium sp. is important, not only from an academic standpoint but also has great ecological significance, especially in limnology. This study examines the growth and productivity of Oedogonium sp. under different light intensity in laboratory scale. The main research includes Oedogonium sp. culture, measurement of growth (doubling time (DT) and relative growth rate (RGR)), measurement of primary productivity, and the effect of light measurement on cells through observation of histological preparations. The results showed that Oedogonium sp. on indoor culture showed negative growth (RGR of -0.054), whereas in semi-outdoor culture showed positive growth (RGR 0.046 - 0.47 g / day and DT for 14.72 - 15.09 days. Primary productivity and respiration in 1000 lux lighting cultures showed greater value than cultures in 650 lux lighting. The length of Oedogonium sp. ranged from 18.68 - 34.47 µm in dark light, 51.33 - 95.00 µm in moderate light, and 48.83 - 70.75 µm in bright light. This study showed that in indoor culture, the availability of light, temperature, pH and DO significantly affect the growth of Oedogonium sp., whereas in semi-outdoor culture, temperature, pH and DO factors was not limiting factors. The difference of light intensity influenced the growth and productivity of Oedogonium sp. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133830804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2020-12-17DOI: 10.14203/BERITABIOLOGI.V19I3A.3863
W. Sari, L. Chiuman, Sahna Ferdinand Ginting, C. Ginting
{"title":"PENGARUH KRIM EKSTRAK JINTAN HITAM (Nigella sativa) TERHADAP KADAR KOLAGEN DAN HIDRASI KULIT PADA TIKUS (Rattus norvegicus) GALUR WISTAR JANTAN YANG DIPAPAR SINAR ULTRAVIOLET-B","authors":"W. Sari, L. Chiuman, Sahna Ferdinand Ginting, C. Ginting","doi":"10.14203/BERITABIOLOGI.V19I3A.3863","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V19I3A.3863","url":null,"abstract":"Photoaging is caused by exposure to sunlight and ultraviolet radiation (UV-B) through the reactive oxygen species (ROS) molecules formation, resulting in decreased levels of collagen. It is characterized by wrinkles, sagging and rough surfaces. Black cumin has been used in medicine due to its high antioxidant potential. The aim of this study is to identify the effect of black cumin cream on collagen levels and hydration of the skin of Rattus norvegicus exposed to ultraviolet-B rays. This is experimental study in which 30 male Wistar strain rats were classified into 6 sub-groups, namely the KN group (without treatment), KP (getting Nivea cream moisturizer), P1 (basic cream ), P2 (black cumin seed extract cream 0.25%), P3 (cream 0.5%) and P4 (cream 0.75%). P4 rat collagen levels increased significantly before and after the intervention (24 ± 2.23 vs 42.6 ± 14.6, p <0.05).Cream with lower concentrations did not provide a significant increase (p> 0.05). P4 rats also showed increased skin hydration (38.6 ± 19.3 to 63.0 ± 4.47 (p <0.05)), and this was not found in other groups. The administration of topical black cumin seed extract 0.75% can inhibit decreased levels of collagen and increase skin tissue hydration. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116661986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2020-09-21DOI: 10.14203/BERITABIOLOGI.V19I3A.3853
Sinta Agustina, I. Wiryawan, S. Suharti
{"title":"THE IMPORTANCE OF RUMEN ANAEROBIC FUNGI ON FIBER DEGRADATION IN RUMINANTS: REVIEW","authors":"Sinta Agustina, I. Wiryawan, S. Suharti","doi":"10.14203/BERITABIOLOGI.V19I3A.3853","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V19I3A.3853","url":null,"abstract":"Forage is a feed source for ruminant livestock, but one of the limiting factors of forage is high content of fiber in old forage plants. The fiber content in forage can only be degraded by rumen microbes. One of the rumen microbes that has fiber degrading activity is rumen anaerobic fungi because it can produce very active enzymes to degrade lignocellulose. The rumen anaerobic fungi are divided into several genera which are grouped base on the number of flagella in zoospores, thallus morphology and rhizoid type. The presence of fungi in the rumen is very important because fungi can form rhizoid which will penetrate the feed particles and degrade plant cell walls physically and chemically. In addition, fungi can produce fiber degrading enzymes such as cellulase, hemicellulase, pectinase, and lignocellulase which can increase feed digestibility. However, in Indonesia there is not much study of the potential for rumen anaerobic fungi, so the aims of this review paper is to discuss the potential of anaerobic fungi rumen in improving fiber digestibility in livestock.","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122767879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2019-08-27DOI: 10.14203/beritabiologi.v18i2.2993
S. H. Nasution, G. S. Haryani, R. Dina, Octavianto Samir
{"title":"ANCAMAN JENIS IKAN ASING LOUHAN TERHADAP IKAN ENDEMIK DI DANAU MATANO, SULAWESI SELATAN","authors":"S. H. Nasution, G. S. Haryani, R. Dina, Octavianto Samir","doi":"10.14203/beritabiologi.v18i2.2993","DOIUrl":"https://doi.org/10.14203/beritabiologi.v18i2.2993","url":null,"abstract":"Lake Matano is one of the ancient lakes in Sulawesi where some endemic fish species of Telmatherina, Mugilogobius, Oryziasand Glossogobius matanensis lived. The presence of alien species can be on threat for the loss of biodiversity in fresh waters, including endemic fish in Lake Matano. The research aim to review the threat of louhan fish as alien species to endemic fish in Lake Matano. The study was conducted in Lake Matano at five stations in April and August 2015 namely Lawa, Nuha, Petea, Pantai Impian, and Pantai Kupu-kupu. Sam-ples were collected using experimental gillnet with mesh size of ¾; 1; 1¼; 1⅟2; 2; 2,5; and 3 inches. Fouteen species were found consist of 10 endemic species and red list.These were three of genus Glossogobius, seven species of Telmatherina, and four alien species of the carp (Cyprinus carpio), louhan, gourami (Trichopodus trichopterus), and tilapia (Oreochromis niloticus). The most common species of endemic fish is T. antoniae. Louhan is the result of a hybrid species Amphilopus citrinellus x Cichlasoma trimaculatum. The abundance of louhan is 64 in April and 40 fishes in August, respectively at a time caught. The percentage ratio of louhan to endemic fish was of 8.6% and 18.3%. This indicated that the louhan fish tend to be invasive and certainly threatened endemic fish survival in this lake. The maturity of gonads of louhan is in the level of I - IV that enables the fish to reproduce quickly.","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127031076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2019-08-27DOI: 10.14203/beritabiologi.v18i2.3689
B. Prasetyo, Tatik Chikmawati, E. B. Walujo, E. A. Zuhud
{"title":"PERSPEKTIF GENDER SUKU OSING DI BANYUWANGI DALAM PENILAIAN KEMANFAATAN TANAMAN","authors":"B. Prasetyo, Tatik Chikmawati, E. B. Walujo, E. A. Zuhud","doi":"10.14203/beritabiologi.v18i2.3689","DOIUrl":"https://doi.org/10.14203/beritabiologi.v18i2.3689","url":null,"abstract":"The different roles and responsibilities of male and female of the Osing tribe influence the knowledge of the use of plants. The aims of research is to analyze the values of the benefits of plants based on gender perception. The research used a qualitative and quantitative methods. Data collection for the value of plant species utilization were carried out using Pebble Distribution Method (PDM) and analysis used Index of Cultural Significance (ICS). The Osing community recognised 86 plant species that were grouped in to eight beneficial groups. The male in three villages have a better understanding of ethnobotanical knowledge and practice than female on crops as staple foods, medicines, fruits, and firewood, while understanding of ornamental plants are relatively the same for both male and female. Female have a broader and more detailed understanding on vegetables, plant species used as building construction, and traditional rituals. The high PDM value in eight beneficial groups was supported by the high value of each ICS. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115091989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}