Frontiers in Environmental Microbiology最新文献

Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou 分离和鉴定 SOCAPALM-Mbambou 公司产生的固体棕榈油萃取污泥中具有净化潜力的本地细菌

Frontiers in Environmental Microbiology Pub Date : 2024-04-17 DOI: 10.11648/j.fem.20241002.11

T. Denis, Simo Claude, T. Désiré

{"title":"Isolation and Characterization of Indigenous Bacteria with Purifying Potential in Solid Palm Oil Extraction Sludge Generated by SOCAPALM-Mbambou","authors":"T. Denis, Simo Claude, T. Désiré","doi":"10.11648/j.fem.20241002.11","DOIUrl":"https://doi.org/10.11648/j.fem.20241002.11","url":null,"abstract":"The oil palm industry contributes significantly to the economic development of producing countries such as Cameroon. Unfortunately, the exploitation of palm oil constitutes a source of environmental pollution due to the production of enormous quantities of waste during its extraction process, including solid sludge generating greenhouse gases which contribute to global warming climatic. All this leads to the search for alternatives which consists of isolating and characterizing indigenous bacteria with biodegradation capacities in sludge from palm oil extraction. The pH and bacterial counts were determined by the potentiometric method and the decimal dilution technique, respectively. The isolated bacteria were identified by their cultural, cellular and biochemical characteristics. In addition, the identification of Gram- bacteria was further explored by the API 20 E gallery. The palm oil biodegradability test was carried out on M2 medium supplemented with 2% palm oil. The solid sludge biodegradability test was carried out on liquid MSM medium supplemented with 4% sludge stock solution. The results showed that the sludge sample had a slightly alkaline pH of 7.3. A bacterial load of around 109 CFU/g of soil was counted. Thirty-one bacterial strains were isolated and purified, including 12 Bacillus sp, 10 Pseudomonas sp, 8 Proteus mirabilis and 1 Klebsiella pneumoniae. All isolates tested for their ability to degrade palm oil or solid sludge grew in culture media with palm oil or solid sludge as the sole source of carbon and energy but with a difference in load. Thus, isolates BI2, BI5, BI31, BI10 and BI 9 showed the highest degradation capacities. These isolates could be used to constitute consortia of microorganisms that can be used in the treatment of waste generated by palm oil extraction.","PeriodicalId":278158,"journal":{"name":"Frontiers in Environmental Microbiology","volume":" 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140690974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal Population Trends of Microbial Communities in Oil Tainted Soils in Greater Port Harcourt Area, Nigeria 尼日利亚大哈科特港地区油污土壤中微生物群落的季节性种群趋势

Frontiers in Environmental Microbiology Pub Date : 2024-02-20 DOI: 10.11648/j.fem.20241001.13

W. P. Muyoma, Boadu Kwasi Opoku, E. N. Wafula, E. Ibisime

{"title":"Seasonal Population Trends of Microbial Communities in Oil Tainted Soils in Greater Port Harcourt Area, Nigeria","authors":"W. P. Muyoma, Boadu Kwasi Opoku, E. N. Wafula, E. Ibisime","doi":"10.11648/j.fem.20241001.13","DOIUrl":"https://doi.org/10.11648/j.fem.20241001.13","url":null,"abstract":"The utilization of oil in industries has devastating effect to the environment. Industrial effluents and oil spills are continuously contaminating the soil. Further, seasonality influences the distribution of pollutants in soil. Consequently, soil microbial biota and ecological processes are affected. This study assessed the effect of seasonality on soil fungal and bacterial communities in oil contaminated soils in 12 selected sites in Greater Port Harcourt Area. Standard analytical procedures were used to obtain bio-physicochemical data from the soil samples and t test was used to analyse data. The levels of total petroleum hydrocarbons (TPH) were above 5000 ppm (DPR recommended limit). There was significant difference (p ≤ 0.000) between the means of TPH in wet and dry seasons. Seasonality influenced % HUF and % HUB in the soils of urban, industrial and agricultural sites. Generally, the seasonality trends showed that there was a declining population of THB, HUB, TF, and HUF from the wet season to the dry season. However, the results show that there was a stable trend in % HUB as compared to oscillations observed in % HUF in oil tainted soils across a seasonal divide. We recommend characterization of the microorganism to identify the best candidate for bioremediation of oil tainted soils across a seasonal divide.","PeriodicalId":278158,"journal":{"name":"Frontiers in Environmental Microbiology","volume":"92 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Qualitative Analysis of Mycotoxins by Thin Layer Chromatography (TLC) 利用薄层色谱法（TLC）对霉菌毒素进行定性分析

Frontiers in Environmental Microbiology Pub Date : 2024-01-18 DOI: 10.11648/j.fem.20241001.11

Sowmya Kengarangappa Lakshman, Ramalingappa Bellibatlu

引用次数: 0

Adoption and Adoption Determinants of Climate Smart Agriculture Practices Among Smallholder Farmers in Welmera District, Oromia Region, Ethiopia 埃塞俄比亚奥罗米亚地区 Welmera 区小农采用气候智能型农业做法及其决定因素

Frontiers in Environmental Microbiology Pub Date : 2023-11-29 DOI: 10.11648/j.fem.20230903.12

Mesay Hailu, Esubalew Abate

引用次数: 0

Properties of Streptomyces Bacteria from the Rhizosphere of Some Halophytes at North-East of Qatar: Al Ghariya Case Study 卡塔尔东北部一些盐生植物根瘤菌的链霉菌特性：Al Ghariya 案例研究

Frontiers in Environmental Microbiology Pub Date : 2023-11-21 DOI: 10.11648/j.fem.20230903.11

Roda Fahad Al-Thani, Aisha Al-Mohannadi, Dana Deyab, Fatima Abdulla Al-Yafei, Mohammad Yousaf Ashfaq, Bassam Taha Yasseen

引用次数: 0

Enhancements of Residual Soil Properties Using Vermicompost and Inoculant Strains in Low-Fertility Sandy Soil

利用蛭石堆肥和接种菌株提高低肥力沙质土壤的残留土壤性质

Frontiers in Environmental Microbiology Pub Date : 2023-10-28 DOI: 10.11648/j.fem.20230902.12

Gezahagn Goshu Abate

引用次数: 0

Lymphocystis Disease Virus in Largemouth Bass - A Case Report 大口鲈中的淋巴囊肿病病毒--病例报告

Frontiers in Environmental Microbiology Pub Date : 2023-06-10 DOI: 10.11648/j.fem.20230902.11

Suja Aarattuthodi

引用次数: 0

Production, Optimization and Characterization of Amylase Using Solid State Fermentation Method 固态发酵法淀粉酶的生产、优化及表征

Frontiers in Environmental Microbiology Pub Date : 2023-05-17 DOI: 10.11648/j.fem.20230901.12

Emmanuel Olabode Ayinde, Timothy Olubisi Adejumo, Olusegun Richard Adeoyo

{"title":"Production, Optimization and Characterization of Amylase Using Solid State Fermentation Method","authors":"Emmanuel Olabode Ayinde, Timothy Olubisi Adejumo, Olusegun Richard Adeoyo","doi":"10.11648/j.fem.20230901.12","DOIUrl":"https://doi.org/10.11648/j.fem.20230901.12","url":null,"abstract":": Amylases are enzymes produced by a wide range of organisms. The applications of these biomolecules cut across different industries such as detergent, brewing and food industries. Aspergillus flavus has been well documented as one the microbes capable of degrading starch-based agricultural waste materials. This study aimed at optimizing and characterizing amylase produced using agro-waste (potato peels) as a sole carbon source. A. flavus was obtained from the Department of Microbiology laboratory, Adekunle Ajasin University, Akungba-Akoko, Ondo State, Nigeria. The fungus was preserved on PDA and stored at 4°C throughout the study period. This was followed by characterization and purification. Preliminary screening result showed that A. flavus had a clear zone of 13.33 mm (diameter of hydrolytic zone) and amylase activity of 545 U/ml. Stability assay revealed that the enzyme was stable at 50°C and 60°C by retaining 91% and 84% residual activities respectively after 4 h of pre-incubation period, while residual activities of 80% and 90% were observed at pH 6.0 and 8.0 respectively. Protein band analysis showed that the amylase from A . flavus had molecular weight of 35 kDa. These results indicated that the amylase produced from A. flavus had good catalytic properties and compared well with those from other known fungi. This study therefore recommends industrial production of amylase from A. flavus using potato peels as a substrate.","PeriodicalId":278158,"journal":{"name":"Frontiers in Environmental Microbiology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123762318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Peanut (Arachis hypogaea L.) Nodulating Rhizobia and Assessment of Their Phosphate Solubilizing Activity 花生(arachhis hypogaea L.)的分离根瘤菌的根瘤化及其溶磷活性的评价

Frontiers in Environmental Microbiology Pub Date : 2023-04-27 DOI: 10.11648/j.fem.20230901.11

Mba Edou Simon Jeremie, Ngo Nkot Laurette, Youagang Gougueu Harris Stephane, Semboung Lang Firmin, Nyaka Ngobissa Aurelie Irene Claire, Timb Sara Augustine Laurence, Asseng Charles Carnot

引用次数: 0

Affecting Factors of Soil Microorganisms and Their Research Prospects 土壤微生物的影响因素及其研究前景

Frontiers in Environmental Microbiology Pub Date : 2021-07-24 DOI: 10.11648/J.FEM.20210703.11

W. Lide, Wu Chunrong, Song Dacheng, Wang Jia, He Hongsheng, G. Chunxiu, Wang Fangling

引用次数: 1