Asian Journal of Biotechnology最新文献_第6页

In vitro germination and pollen conservation of sorne Musa species 槐属植物离体萌发及花粉保存研究

Asian Journal of Biotechnology Pub Date : 2011-06-01 DOI: 10.3923/AJBKR.2011.554.563

E. Youmbi, Nanga Jean Philipps Fonkam, K. Tomekpé, C. Fonbah

{"title":"In vitro germination and pollen conservation of sorne Musa species","authors":"E. Youmbi, Nanga Jean Philipps Fonkam, K. Tomekpé, C. Fonbah","doi":"10.3923/AJBKR.2011.554.563","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.554.563","url":null,"abstract":"The yield ofbanana and plantain which is an important food is affected especially by black leaf streak disease. Pollen from resistant wild types is used by conventional breeding to create new hybrids resistant to black leaf streak disease. A strategy of pollen genetic resource preservation and storage is necessary for this breeding. The main objective of this study was to optimize the conditions for in vitro germination, conservation and storage of pollen from three ban ana diploids (Musa acuminata). Pollen grains used in this study were collected from Njombe and Ekona banana collections. The basal media used for in vitro culture were th ose of Heslop-Harrison and Brewbaker and Kwack. Different temperature and pH values tested allowed for the evaluation of germination rates. Pollen were desiccated and stored in a freezer ( -20°C) and in a refrigera tor ( + 10°C). The results obtained showed that the basal medium of Heslop-Harrison supplemented with 5% sucrose allowed to obtain optimum pollen germination rate. The optimum temperature was 30°C and the optimum pH values were 6.5 for Calcutta and 6.8 for M53 and Zebrina. The pollen grains of the three varieties can be conserved freshly in the refrigerator at+ 10°C for at !east two months. These results are very important for ban ana and plantain breeding programs because the conditions of pollen storage for the three varieties are weil established and permit genetic material sharing and diffusion between different ban ana research centers. (Resume d'auteur)","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"51 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116006058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Application of an acclimated microbial consortium as biocatalyst for rapid determination of biochemical oxygen demand 驯化菌群作为生物催化剂在生化需氧量快速测定中的应用

Asian Journal of Biotechnology Pub Date : 2011-05-01 DOI: 10.3923/AJBKR.2011.519.529

S. Shahir, S. L. Chun, R. Ahamad

{"title":"Application of an acclimated microbial consortium as biocatalyst for rapid determination of biochemical oxygen demand","authors":"S. Shahir, S. L. Chun, R. Ahamad","doi":"10.3923/AJBKR.2011.519.529","DOIUrl":"https://doi.org/10.3923/AJBKR.2011.519.529","url":null,"abstract":"Biochemical Oxygen Demand (BOD) is an important parameter for water pollution monitoring. Since the conventional BOD assay by American Public Health Association (APHA) takes 5 days to complete (termed BOD5), development of an alternative method that can rapidly measure the amount of biodegradable organics in wastewater is highly desirable. In the present study, single and mixed microbial cultures isolated from different environments in Malaysia were tested as potential biocatalyst in an assay called Ferricyanide Mediated-Biochemical Oxygen Demand (FM-BOD) used for rapid BOD detection. Unlike the BOD5 assay, the FM-BOD is based on biochemical degradation of organic compounds via reduction of ferricyanide as an alternative terminal electron acceptor to dissolved oxygen. The increase in concentration of microbially produced ferrocyanide during incubation of the microorganisms in the presence of ferricyanide and organic substrates was measured voltammetrically using a 10 µm platinum working electrode. Measurements were conducted using linear sweep voltammetry at potential 0-600 mV versus Ag/AgCl reference electrode. Limiting currents were determined by voltammetry at Eapp = +450 mV (vs Ag/AgCl). The amount of ferrocyanide produced was found to be proportional to the amount of biodegradable organics. From the study, it is shown that mixed microbial consortium acted as a better biocatalyst for rapid BOD detection compared to both single cultures and a commercial consortium. The microbial consortium consists of four species of microorganisms. FM-BOD assays were conducted in optimized conditions with microbial consortium at final absorbance of OD600 2.5 and ferricyanide at concentration 60 mM. Results showed that the FM-BOD assay was able to detect biodegradable organics in 1 h compared to the current BOD5 assay of 5 days. This FM-BOD assay with optimized conditions is applicable to food wastewater consisting of easily degradable carbon source.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115845616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Anther Culture Response in Boro Rice Hybrids 杂交稻花药培养的响应

Asian Journal of Biotechnology Pub Date : 2011-05-01 DOI: 10.3923/AJBKR.2011.470.477

C. Sen, R. Singh

引用次数: 9

Construction of a Recombinant Fab Fragment of a Monoclonal Antibody against Leptin Receptor 瘦素受体单克隆抗体Fab片段的构建

Asian Journal of Biotechnology Pub Date : 2011-05-01 DOI: 10.3923/AJBKR.2011.493.506

Milad Mohkam, Sayyed Hamid Zarkesh-Es, M. Fazeli

引用次数: 2

Production of Cold-active Extracellular a-Amylase by Newly Isolated Microbacterium foliorum GA2 from Gangotri glacier, Western Himalaya, India 从印度西喜马拉雅地区Gangotri冰川新分离的叶状微细菌GA2生产冷活性胞外a-淀粉酶

Asian Journal of Biotechnology Pub Date : 2011-05-01 DOI: 10.3923/AJBKR.2011.449.459

Mojtaba Roohi, I. Ahmad, J. Arif

引用次数: 18

Purification and Properties of Novel Malate Dehydrogenase Isolated from Pseudomonas aeruginosa 铜绿假单胞菌苹果酸脱氢酶的纯化及性质研究

Asian Journal of Biotechnology Pub Date : 2011-05-01 DOI: 10.3923/AJBKR.2011.478.485

P. Vijayaragh, Surgen A. Bright, L. AnujNishanth, S. Vincent

引用次数: 3

Diversity of Vibrio spp. at the Andaman Tarutao Island, Thailand 泰国安达曼塔鲁涛岛弧菌的多样性

Asian Journal of Biotechnology Pub Date : 2011-05-01 DOI: 10.3923/AJBKR.2011.530.539

U. Thongchank, P. Sukhumungo, P. Mitraparp, K. Srinitiwar, S. Plathong, V. Vuddhakul

引用次数: 4

Assessment of genetic diversity and its usefulness for varietal identification in Indian elite varieties of wheat (T. aestivum L. em thell.) using RAPD markers. 利用RAPD标记评价印度小麦优良品种(T. aestivum L. em thell.)的遗传多样性及其品种鉴定。

Asian Journal of Biotechnology Pub Date : 2011-05-01 DOI: 10.3923/AJBKR.2011.460.469

Reetesh Kumar, S. Jaiswal, M. Vishwakarma, V. P. Rai, P. Upadhyay, A. Joshi

引用次数: 8

Effect of Low Temperature, Radiation and their Combination Treatments on Microorganisms Associated with Fresh Water Mola Fish, Amblypharyngodon mola (Hamilton-Buchanan 1822) 低温、辐射及其联合处理对淡水翻齿鱼(amblyphyngodon Mola)相关微生物的影响(Hamilton-Buchanan 1822)

Asian Journal of Biotechnology Pub Date : 2011-05-01 DOI: 10.3923/AJBKR.2011.507.518

A. Mahin, Md. Golam Sorrowar, Md.Zahid Hasan, M. Haque, H. Rashid

引用次数: 6

Molecular Modeling and Structural Analysis of Five SE Clan (S12 Family) Serine Proteases 5个SE族(S12家族)丝氨酸蛋白酶的分子建模和结构分析

Asian Journal of Biotechnology Pub Date : 2011-05-01 DOI: 10.3923/AJBKR.2011.435.448

Aparna Laskar, Sirshendu Chatterjee, A. Roy, S. Dey, C. Mandal

引用次数: 4