Asian Journal of Biotechnology最新文献_第4页

Adventitious Rooting and Proliferation from Different Explants of Citrullus colocynthis (L.) Schrad an Endangered Medicinally Important Cucurbit 甜瓜不同外植体的不定根及增殖一种具有重要药用价值的濒危葫芦

Asian Journal of Biotechnology Pub Date : 2015-02-01 DOI: 10.3923/AJBKR.2015.88.95

D. Krishna, T. Shasthree

{"title":"Adventitious Rooting and Proliferation from Different Explants of Citrullus colocynthis (L.) Schrad an Endangered Medicinally Important Cucurbit","authors":"D. Krishna, T. Shasthree","doi":"10.3923/AJBKR.2015.88.95","DOIUrl":"https://doi.org/10.3923/AJBKR.2015.88.95","url":null,"abstract":"An efficient procedure has been developed for induction of adventitious rooting and proliferation from different explants of Citrullus colocynthis. Leaf, stem, cotyledon and hypocotyledon explants cultured on different concentration of auxins and cytokinins, were supplemented on MS medium to test their ability to induce rhizogenesis. Murashige and Skoog (MS) medium fortified with 2,4-dichlorophenoxyacetic (2,4-D), indoleacetic acid (IAA), Indole-Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA). Each at the concentrations of 0, 0.5, 1.0, 1.5, 2.0 and 2.5 mg LG were evaluated for their effects on adventitious root induction in the present study. High concentration of IAA (2.0 mg LG) and low concentration of IBA (1.5 mg LG) is best for induction of rhizogenesis. High concentration of IBA favored formation of malformed and thick roots. The NAA in combination with IBA is most potential in inducing high percentage of rooting (50%) and IAA in combination with IBA induced maximum rooting 62% with highest number of roots per shoot (3.80±0.2) and root length (3.8±0.1 cm) were obtained from leaf, cotyledon and stem than hypocotyls. Results revealed that, the best rooting ability was observed from in stem explants cultured on MS medium supplemented with 2.0 mg LG IAA and 1.5 mg LG IBA, multiple roots fibrous were produced from cotyledon explants on MS fortified with 2.0 mg LG 2,4-d and multiple roots cluster produced from leaf explants 1.5 mg LG IBA and 2.0 mg LG IAA. A distinct feature of this study is the adventitious rooting and proliferations from different explants of Citrullus colocynthis which has not been reported previously.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129916117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Effect of Impeller Number and Position on Growth Yield and Virulence of Bordetella pertussis Strain 509 During Large Scale Batch Fermentor Cultivation 大规模间歇发酵罐培养中叶轮数量和位置对百日咳杆菌509株生长产量和毒力的影响

Asian Journal of Biotechnology Pub Date : 2015-02-01 DOI: 10.3923/AJBKR.2015.60.70

K. Shivananda, S. Jagannatha, S. Lokesh, R. Vijayakuma, K. Mani

{"title":"Effect of Impeller Number and Position on Growth Yield and Virulence of Bordetella pertussis Strain 509 During Large Scale Batch Fermentor Cultivation","authors":"K. Shivananda, S. Jagannatha, S. Lokesh, R. Vijayakuma, K. Mani","doi":"10.3923/AJBKR.2015.60.70","DOIUrl":"https://doi.org/10.3923/AJBKR.2015.60.70","url":null,"abstract":"Studies have been undertaken to investigate, the effect of impeller number and its position upon the growth yield of Bordetella pertussis strain 509 during large scale batch cultivation. It was shown that, the maximum growth yield opacity was found to be 70 IOU mLG at 48 h cultivation in single impeller fixed at the bottom position of fermentor vessel. Whereas the optimal and moderate culture opacity 60 and 50 IOU mLG was observed during the two impellers mounted at two different positions on the shaft. Similarly the least growth yield was obtained during three impeller located at top, middle and bottom region of the shaft and the final opacity was found to be 30 IOU mLG at the end of 48 h cultivation with an increasing of culture pH 8.02. However, in two impeller combination experimental study also showed moderate growth yield at 48 h could give to homogenized mixing and good aeration. Thus, the two different location of the impeller combination influences two vortex flows, enhances optimal growth rate, viz; the lower impeller act as gas disperser and creates radial velocity with higher rates of air dispersion, whereas the upper impeller pumps the broth towards down thus the combined agitation of the broth leads to good mixing and even transfer of Dissolved Oxygen (DO2) to the growing cells and found moderate bacterial growth. In the combination of three impellers mounted at three different positions experimental study shown that, lesser growth yield at the end of 48 h cultivation, due to higher forth accumulation.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123316978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Genetic Diversity between Populations of the Genus Schistura McClelland from the Garhwal and Kumaun Region Using RAPD Marker 利用RAPD标记分析加尔瓦尔和库曼地区麦克莱兰血吸虫属居群的遗传多样性

Asian Journal of Biotechnology Pub Date : 2015-02-01 DOI: 10.3923/AJBKR.2015.71.79

R. K. Negi, B. Joshi

{"title":"Genetic Diversity between Populations of the Genus Schistura McClelland from the Garhwal and Kumaun Region Using RAPD Marker","authors":"R. K. Negi, B. Joshi","doi":"10.3923/AJBKR.2015.71.79","DOIUrl":"https://doi.org/10.3923/AJBKR.2015.71.79","url":null,"abstract":"Genetic based study provide emphasis on the population study of geographically isolated population, gene flow among them and evolutionary history of the species. In the present study, we aimed to detect the genetic variations in species of genus Schistura from Uttarakhand. Very little is known for the pattern of distribution and genetic diversity of the species of genus Schistura from the Garhwal and Kumaun region. Out of six primer only four primers were used to generate the fragments patterns from the samples collected. Polymorphism within and between the populations were assayed and total 60 bands were amplified ranging from 1-150 kb. Maximum number of bands was observed in Schistura montanus from the Srinagar, Garhwal and Uttarkashi regions. Total 60 bands were amplified in the all four primer with high percentage of polymorphic loci 50% Schistura montanus from Garhwal region and 28.8% in the S. montanus from the Kumaun region were observed. The level of heterozygosity were found 0.006-0.18 in the all three species. The percentage of polymorphism was 50% within the populations and high heterozygosity the sample of S. montanus when compared with other species. Observed pattern of RAPD markers reveals that the samples from the Garhwal region exhibit high diversity and used four primers are sufficient to distinguish the different population in the both region except the samples of the S. gangiticus of Garhwal region.","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"47 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132935190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Optimizing Sterilization Condition for the Initiation of Sweet Potato (Ipomoea batatas) Culture in vitro 甘薯离体培养起始灭菌条件的优化

Asian Journal of Biotechnology Pub Date : 2014-02-01 DOI: 10.3923/AJBKR.2014.25.37

R. Hammond, J. Buah, P. A. Asare, S. Acheampong

引用次数: 8

Citric acid production from cellulase-digested palm oil mill effluent. 纤维素酶消化棕榈油厂废水生产柠檬酸。

Asian Journal of Biotechnology Pub Date : 2013-02-01 DOI: 10.3923/AJBKR.2013.51.60

C. Nwuche, H. Aoyagi, J. Ogbonna

引用次数: 7

Metagenomics: a promising approach to assess enzymes biocatalyst for biofuel production. 宏基因组学:一种评估生物燃料生产中酶生物催化剂的有前途的方法。

Asian Journal of Biotechnology Pub Date : 2013-02-01 DOI: 10.3923/AJBKR.2013.33.50

R. Sebastian, Kim Jaeyoung, Kim Taehun, Lee Kyungtai

引用次数: 11

Decolorization of Synthetic Dyes Using Bacteria Isolated from Textile Industry Effluent 利用纺织工业废水中分离的细菌对合成染料进行脱色

Asian Journal of Biotechnology Pub Date : 2012-03-01 DOI: 10.3923/AJBKR.2012.129.136

K. R. Mahbub, A. Mohammad, M. Ahmed, S. Begum

引用次数: 22

Comparative Study of Microbial Fuel Cell for Electricity Generation by Enriched Exoelectron Generating Bacteria from Environmental Samples 环境样品富集产外电子细菌发电微生物燃料电池的比较研究

Asian Journal of Biotechnology Pub Date : 2012-03-01 DOI: 10.3923/AJBKR.2012.137.142

Pratima Gupta, Piyush Parkhey, Komal Joshi, Anjali Mahilkar, Jasjeet Kaur Bhatia, L. Meena

引用次数: 4

In vitro Micropropagation using Corm Bud Explants: An Endangered Medicinal Plant of Gloriosa superba L. 濒危药用植物金凤花球茎外植体离体微繁研究。

Asian Journal of Biotechnology Pub Date : 2012-03-01 DOI: 10.3923/AJBKR.2012.120.128

A. Arumugam, K. Gopinath

引用次数: 27

Regeneration of Plantlets from Leaf Derived Callus of Aerva lanata, Juss: A Medicinal Plant 药用植物水芹叶片愈伤组织再生植株的研究

Asian Journal of Biotechnology Pub Date : 2012-03-01 DOI: 10.3923/AJBKR.2012.143.146

Prakasha, K. Rave, Kabballi Balakrishn, G. Lokesh

引用次数: 3