{"title":"Molecular confirmation of the occurrence of Heterodera sojae in Japan","authors":"Hiromichi Sakai, A. Kushida","doi":"10.3725/jjn.49.29","DOIUrl":"https://doi.org/10.3725/jjn.49.29","url":null,"abstract":"","PeriodicalId":271755,"journal":{"name":"Japanese Journal of Nematology","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121659092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Uesugi, K. Adachi, Gaku Murata, Hattori Taichiro, Y. Arakawa, Takayuki Suzuki
{"title":"Effect of plant-parasitic nematodes on initial growth of sugarcane in Tanegashima Island","authors":"K. Uesugi, K. Adachi, Gaku Murata, Hattori Taichiro, Y. Arakawa, Takayuki Suzuki","doi":"10.3725/jjn.49.39","DOIUrl":"https://doi.org/10.3725/jjn.49.39","url":null,"abstract":"","PeriodicalId":271755,"journal":{"name":"Japanese Journal of Nematology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129212656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gaku Murata, K. Uesugi, K. Adachi, Hattori Taichiro, Y. Arakawa, Takayuki Suzuki
{"title":"Plant-parasitic nematodes detected from sugarcane fields in Tanegashima Island","authors":"Gaku Murata, K. Uesugi, K. Adachi, Hattori Taichiro, Y. Arakawa, Takayuki Suzuki","doi":"10.3725/jjn.49.35","DOIUrl":"https://doi.org/10.3725/jjn.49.35","url":null,"abstract":"","PeriodicalId":271755,"journal":{"name":"Japanese Journal of Nematology","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115936323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hiroaki Tabuchi, T. Kuranouchi, A. Kobâyashi, Y. Monden, Kazuki Kishimoto, M. Tahara, Y. Okada, H. Iwahori
{"title":"Southern root-knot nematode race SP6 is divided into two races","authors":"Hiroaki Tabuchi, T. Kuranouchi, A. Kobâyashi, Y. Monden, Kazuki Kishimoto, M. Tahara, Y. Okada, H. Iwahori","doi":"10.3725/JJN.47.29","DOIUrl":"https://doi.org/10.3725/JJN.47.29","url":null,"abstract":"","PeriodicalId":271755,"journal":{"name":"Japanese Journal of Nematology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129437369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Difference in Meloidogyne incognita propagation among ground cherry species","authors":"H. Okada, S. Sekimoto, M. Araki","doi":"10.3725/JJN.47.25","DOIUrl":"https://doi.org/10.3725/JJN.47.25","url":null,"abstract":"","PeriodicalId":271755,"journal":{"name":"Japanese Journal of Nematology","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134275887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Soboliphyme baturini (Nematoda: Dioctophymatidae) infection in a domestic cat suffering severe diarrhea","authors":"M. Asakawa, M. Takeuchi, Yoshikazu Torii","doi":"10.3725/JJN.47.21","DOIUrl":"https://doi.org/10.3725/JJN.47.21","url":null,"abstract":"The genus Soboliphyme (Nematoda: Enoplea: Dorylaimia: Dioctophymatida: Dioctophymatina: Soboliphymatidae) Petrov, 1930 (De Ley and Blaxter, 2002) is a group of parasitic nematodes found in the families Soricidae and Musteridae in the Holarctic Region, including Japan (Asakawa et al., 1988; Bezdek, 1942; Karpenko et al., 2007; Sato et al., 1999). We isolated a Soboliphyme nematode from a domestic cat (Felis catus: Felidae) when we dewormed it. This is the first local record of the nematode parasitizing a cat in Japan. In November 2015, an immature male cat (ca. 5 months old, body weight 1.25 kg) kept in Achi Village, Nagano Prefecture (35°26′37.7′′N, 20°15′20′′E) suffered severe diarrhea with blood and several tapeworm-like substances. The cat was taken to an animal hospital, and administered Droncit R (Bayer Yakuhin, Ltd, Tokyo, Japan; 5.7 mg/kg, s.c. praziquantel). About a month later, its symptoms almost disappeared, but some trichuridor capillarid-like eggs, 97 μm in length and 30 μm in width, were detected in a fecal examination. Thus, MilbemycinRA (Novartis Animal Health K. K., Kobe, Japan; 0.5 mg/kg milbemycin oxime p.o.) was given. However, because the eggs were still present in the feces, BroadlineR (Nippon Zenyaku Kogyo Co., Ltd, Koriyama, Japan; 0.9 ml, s.c., including 83.0 mg/kg f ipronil, 100 mg/kg (S)-methoprene, 83.0 mg/kg pra z iqu antel , 4.0 mg / kg epr i nomect i n), was administered; soon after, six nematodes were extracted from the patient. No eggs were observed at this time. The parasitic nematodes were fixed and preserved in 70% ethanol, and examined microscopically in lactophenol solution. Measurements and drawings of the nematodes were made with the aid of a microscope drawing attachment (Olympus Model BH2-DA). The specimens were deposited in the Wild Animal Medical Center, Rakuno Gakuen University, Hokkaido, Japan (WAMC/AS no. 15890). All the nematodes collected were female. In the five nematodes measured, the body length was 2.5–3.9 cm (mean 3.36 ± 0.56 cm) and the width 1.2–1.6 mm (mean 1.44 ± 0.13 mm). A well-developed sucker was observed in the anterior part of the body, with a diameter of 1.1–3.3 mm (mean 2.47 ± 0.74 mm, Fig. 1). Based on its peculiar morphology and measurements, the nematode was identified as Soboliphyme baturini (Bezdek, 1942; Karpenko et al., 2007; Sato et al., 1999). Soboliphyme baturini has mainly been recorded in musterid mammals, not only in the Japanese endemic species, Mustela ten, but also in the North American species M. vison (Asakawa et al., 1988, 2009). However, this nematode species has occasionally been found in domestic cats in Europe (Koehler et al., 2007). Therefore, although this is not the first case of detection in a cat, it is","PeriodicalId":271755,"journal":{"name":"Japanese Journal of Nematology","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130589494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reproductive ability of three Japanese populations of Heterodera trifolii on five crops and three carnation cultivars","authors":"S. Sekimoto, T. Uehara","doi":"10.3725/JJN.47.15","DOIUrl":"https://doi.org/10.3725/JJN.47.15","url":null,"abstract":"Three Japanese populations of the clover cyst nematode Heterodera trifolii were examined for their ability to reproduce on five crops and three carnation cultivars. All three populations produced cysts on white clover, azuki bean, spinach, and carnation cvs. Barbara, Cherry Tessino, and Peachy Mambo. No cysts were produced on sugar beet or broccoli. The number of cysts from the Nagano population was significantly higher than that from the Hokkaido and Aomori populations on all plants, except for white clover. The number of cysts from all populations was significantly lower on carnation cv. Barbara than on cvs. Cherry Tessino and Peachy Mambo. These results suggested that carnation cv. Barbara may be the resistant type. Variation in cyst number of the three populations on spinach and carnation cvs. Cherry Tessino and Peachy Mambo indicated variation in reproductive ability on different host plants in Japanese H. trifolii populations. Nematol. Res. 47(1), 15–19 (2017).","PeriodicalId":271755,"journal":{"name":"Japanese Journal of Nematology","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123346049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diagnosis and quantification of the pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhner), in wood of Pinus thunbergii with real-time PCR","authors":"Y. Takeuchi, K. Futai","doi":"10.3725/JJN.39.9","DOIUrl":"https://doi.org/10.3725/JJN.39.9","url":null,"abstract":"A new diagnostic and quantitative real-time PCR assay was developed for the pine wood nematode, Bursaphelenchus xylophilus, which causes a serious epidemic wilt disease of susceptible landscape and forest pine trees. One, 10, 50, 100, 200, and 400 individuals of B. xylophilus in 100 mg of pine tissues were detected and quantified with two sets of primers coding the rDNA-ITS region and β-tubulin (tbb) gene of B. xylophilus, avoiding the need to extract nematodes with Baermann funnels. The assay was efficient, specific for pine wood nematode and sensitive enough to detect a single individual in pine tissues. To test the applicability of the assay for infested hosts, seedlings of the susceptible host species, Pinus thunbergii, were inoculated with B. xylophilus, and the dispersal and multiplication of B. xylophilus inside the seedlings were monitored by both Baermann funnel extraction and realtime PCR. Quantification by real-time PCR gave similar trends in estimates of the nematode population changes inside the host to those obtained by Baermann funnel extraction, although the size of the population estimated was larger for the new method than for the Baermann funnel extraction. The method developed in this study, which was expected to detect and quantify all life stages of the pine wood nematode should be useful for quarantine and field diagnosis.","PeriodicalId":271755,"journal":{"name":"Japanese Journal of Nematology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128887696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}