Journal of World Mitochondria Society最新文献

筛选
英文 中文
REGULATORY MECHANISMS OF DYNAMIN-RELATED PROTEIN 1 (DRP1) AND ITS INFLUENCE ON APOPTOSIS IN BREAST CANCER 动力蛋白相关蛋白1 (drp1)的调控机制及其对乳腺癌细胞凋亡的影响
Journal of World Mitochondria Society Pub Date : 2015-09-07 DOI: 10.18143/JWMS_V2I2_1952
Kayt Hawley, Zachary J G Gardner, Adriana Ramos, Keren Angeles, Emily R. Roberts, K. Craig
{"title":"REGULATORY MECHANISMS OF DYNAMIN-RELATED PROTEIN 1 (DRP1) AND ITS INFLUENCE ON APOPTOSIS IN BREAST CANCER","authors":"Kayt Hawley, Zachary J G Gardner, Adriana Ramos, Keren Angeles, Emily R. Roberts, K. Craig","doi":"10.18143/JWMS_V2I2_1952","DOIUrl":"https://doi.org/10.18143/JWMS_V2I2_1952","url":null,"abstract":"Our study examined the regulation of dynamin-related protein 1 (Drp1) and its influence on apoptosis in breast cancer. Mitochondrial length analyses determined that HTB-22 adenocarcinoma cells displayed fragmented mitochondrial morphology. Western blot analyses of mitochondrial dynamics proteins showed increased endogenous Drp1 and Opa1 expression. This suggests that Drp1 function may be upregulated and/or the balance to fission is lost by the overexpression of Opa1, thus creating a hyper-fused morphology that resembles a pro-fission state. Multiple techniques revealed the majority of Drp1 protein is sequestered in the cytoplasm of these cells. Also, cytochrome c protein expression was predominantly localized to the mitochondria in HTB-22 cells, which upholds a hallmark of cancer, showing apoptotic resistance. To better understand which part of the intrinsic apoptotic pathway is impeded in HTB-22 cells, we examined Drp1 regulatory mechanisms. Post-translational modifications of Drp1, such as phosphorylation of serine residue 637, inhibit its translocation to the mitochondria to initiate fission and begin early stages of apoptosis. Our phosphopurification data suggest that HTB-22 cells have increased phosphorylation at Drp1 S637.  The negative regulation of Drp1-dependent fission by phosphorylation could be the mechanism by which HTB-22 cells can prohibit mitochondrial membrane changes that subsequently promote apoptosis.","PeriodicalId":266249,"journal":{"name":"Journal of World Mitochondria Society","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115224103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信