{"title":"Genome-wide assessment and characterization of simple sequence repeats (SSRs) makers for Capoeta aculeata (Valenciennes, 1844) using NGS data","authors":"H. Gandomkar, S. Nazari, P. Hosseini, H. Abdolhay","doi":"10.52547/injoar.2.2.16","DOIUrl":"https://doi.org/10.52547/injoar.2.2.16","url":null,"abstract":"","PeriodicalId":255746,"journal":{"name":"International Journal of Environmental Research and Education","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126073520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Haghighi, M. Bahmani, A. Yousefi Jourdeh, A. Zamini
{"title":"Effects of super nutrients on some growth and biochemical indices in Siberian sturgeon (Acipenser baerii)","authors":"H. Haghighi, M. Bahmani, A. Yousefi Jourdeh, A. Zamini","doi":"10.52547/injoar.2.2.1","DOIUrl":"https://doi.org/10.52547/injoar.2.2.1","url":null,"abstract":"Super nutrients are nutrient compounds including fat dissolved vitamins, necessary minerals and growth promoters. Present research was carried out with the aim of determining super nutrients effect on some growth and biochemical indices in juvenile Acipenser baerii in International Sturgeon Research Institute during 6 months. Blood samples obtained in days 45 (stage I) and 90 (stage II) and plasma provided. Some plasma biochemical an ions levels were measured in VIRO MED laboratory in Rasht. The results showed that the mean final weight, final biomass, body weight increasing (BWI), specific growth rate (SGR), condition factor condition factor (CF) and daily growth rate (ADG) showed significant difference between control group with other treatments ( p <0.05). Results showed that sodium, potassium and calcium ion levels showed no significant difference in various concentrations of super nutrients during rearing period ( p >0.05). Based on obtained results, super nutrients adding in diet of Acipenser baerii caused to significant difference in growth indices and improved them. Therefore, it recommended using of super nutrients in Siberian sturgeon diet.","PeriodicalId":255746,"journal":{"name":"International Journal of Environmental Research and Education","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114819608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Gandomkar, S. Nazari, M.M. Salahi Ardakani, E. Kazemi
{"title":"Comparison of genome size of diploid and tetraploid of rainbow trout (Oncorhynchus mykiss)","authors":"H. Gandomkar, S. Nazari, M.M. Salahi Ardakani, E. Kazemi","doi":"10.52547/injoar.2.2.7","DOIUrl":"https://doi.org/10.52547/injoar.2.2.7","url":null,"abstract":"Tetraploidization of rainbow trout ( Oncorhynchus mikiss ) was induced by hyperthermia and designed in three treatments (28ºC for 10, 12 and 14 min), 7 h after fertilization. Immature fishes were anaesthetised using standard method in tank (Table 1). Blood samples were collected by caudal venipuncture using 2 mL syringes fitted with 0.5 x 35 mm (25 gauge) needles (Sima, Iran) pre-dosed with heparin (Caspian Tamin, Iran). Blood samples subjected to various conditions, were analysed by FC. Methodology was adapted from established protocols for human blood FC analysis, but modified as required due to the nucleated nature of fish erythrocytes. Blood sample evaluations by FC were performed in surface-deactivated polypropylene tubes to minimize cell-tube adhesion and associated quantitation error. Five millilitre 12 x 75 mm polypropylene Falcon tubes (Maxwell, Italy) were filled with a 4% solution of bovine serum albumin (BSA; Sigma, USA) in PBS and stored overnight at 4°C (Lecommandeur et al., 1994). Prior to use, the tubes were emptied and centrifuged at 2000 rpm for 5 min. For each treatment, we also measured the size of erythrocytes and genome size. Genome size was positively correlated with erythrocyte nucleus size and chromosome number when using PI as the fluorescent dye. This work provides new knowledge on Oncorhynchus mykiss genetics/genomics, important for future research in basic cellular/molecular mechanisms and for the development of molecular techniques in this species. However, further investigation is required to obtain a high percent tetraploid Rainbow trout population.","PeriodicalId":255746,"journal":{"name":"International Journal of Environmental Research and Education","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124901198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Investigation of sexual maturity and change in gonadosomatic index of sex riversed rainbow trout (Oncorhynchus mykiss) treated by oral 17 alpha methyl testosterone","authors":"A. Rastiannasab, E. Kazemi","doi":"10.52547/injoar.2.2.11","DOIUrl":"https://doi.org/10.52547/injoar.2.2.11","url":null,"abstract":"The study was carried out in the genetics and breeding resaerch canter of yasouj. All eye-stage eggs were used for the experiment using vertical hatching units to complete hatching. A standard commercial granule feed was used in all experimental groups. The experiment consisted of control treatment and one dose of hormone 17α-Methyltestosterone added to the diet at the rate of 3 mg per kg food by dissolving in 200 ml of 95% ethanol per kg feed.The use of oral administration of 17α-methyltestosterone (17MT) at 3 mg 17MT/kg of feed for 70 days following the onset of feeding to sex reverse rainbow trout ( Oncorhynchus mykiss ) to produce XX males is well established. Examination of the results showed that 17α- methyltestosterone was effective in sex riversed of rainbow trout. The results has shown in nearly 100% of the treated animals being males. The GSI index in female and male fish of control group were 0.04±0.1 and 0.07±0.01, respectively (Fig.3) and significantly different ( p ≤5%). But GSI index of 100% sex riversed male fish of MT treated was 0.06±0.01 and hadn,t significantly differene ( p ≥5%) with treated group ( genetically male and female).","PeriodicalId":255746,"journal":{"name":"International Journal of Environmental Research and Education","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115437111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inventory of marine molluscs in Gulf of Oran \u0000(Western Algerian coastline)","authors":"A. Kerfouf, F. Kies, S. Boucetta, F. Denis","doi":"10.52547/injoere.3.1.17","DOIUrl":"https://doi.org/10.52547/injoere.3.1.17","url":null,"abstract":"This present paper made it possible to update the inventory of benthic malacofauna in the soft bottoms of gulf of Oran. Samples of macrobenthic fauna were collected and separated from the Molluscs from other zoological groups. 116 Molluscs inventoried and determined by the species, including 13 orders, 20 families, 24 genuses and 29 species. Bivalvia is represented better than Gastropoda with respectively, 24 and 5 species. The analyzed taxa highlighted the dominant and main species on the bottom of the study sites, including Nuculana commutata , which appeared as the major species, followed by Nucula sulcata with respectively 23,46% and 12,34%. The present inventory indicates that the malacological fauna is less diversified than in the other zones studied of the Algerian coast.","PeriodicalId":255746,"journal":{"name":"International Journal of Environmental Research and Education","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115374643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}