Proceeding ICMA-SURE最新文献

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National Economic Recovery (PEN) Through Data Strengthening and Digitization of General Trade in Micro, Small, and Medium Enterprises (UMKM) in Indonesia 通过加强印尼中小微企业(UMKM)一般贸易的数据和数字化实现国民经济复苏
Proceeding ICMA-SURE Pub Date : 2023-04-10 DOI: 10.20884/2.procicma.2023.2.1.8473
I. R. Bawono, R. Naufalin, D. S. Gunawan, W. Novandari, S. Maryani, Jajang Jajang, E. S. Rahmajati
{"title":"National Economic Recovery (PEN) Through Data Strengthening and Digitization of General Trade in Micro, Small, and Medium Enterprises (UMKM) in Indonesia","authors":"I. R. Bawono, R. Naufalin, D. S. Gunawan, W. Novandari, S. Maryani, Jajang Jajang, E. S. Rahmajati","doi":"10.20884/2.procicma.2023.2.1.8473","DOIUrl":"https://doi.org/10.20884/2.procicma.2023.2.1.8473","url":null,"abstract":"MSMEs are one of the actors that play a role in the Indonesian economy. The occurrence of Covid-19 greatly impacted the performance of MSMEs. National Economic Recovery (PEN) through strengthening data and digitizing MSMEs is an important thing to do. The aim is to minimize the impact of losses experienced by MSMEs actors if their products are not sold. However, this effort has several obstacles including human resource problems, infrastructure and information systems, network problems and social & behavioral problems.","PeriodicalId":255709,"journal":{"name":"Proceeding ICMA-SURE","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116220164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Effect of Celery Ethanol Extract on Proteinuria in Unilateral Ureter Obstruction Rat Model 芹菜乙醇提取物对单侧输尿管梗阻模型大鼠蛋白尿的影响
Proceeding ICMA-SURE Pub Date : 2023-04-05 DOI: 10.20884/2.procicma.2023.2.1.7735
Shafira Audy Prameswari, Yoana Rizki Deviriandra, Ariadne Hapsari Putri Taufik, Rizky Aliyah Putri, Athaya Helia Untari, A. Afifah
{"title":"The Effect of Celery Ethanol Extract on Proteinuria in Unilateral Ureter Obstruction Rat Model","authors":"Shafira Audy Prameswari, Yoana Rizki Deviriandra, Ariadne Hapsari Putri Taufik, Rizky Aliyah Putri, Athaya Helia Untari, A. Afifah","doi":"10.20884/2.procicma.2023.2.1.7735","DOIUrl":"https://doi.org/10.20884/2.procicma.2023.2.1.7735","url":null,"abstract":"Chronic kidney disease (CKD) is a condition of decreased kidney function that occurs for more than three months. Proteinuria is one of the markers of impaired kidney function, especially in the renal filtration process. Unilateral ureter obstruction causes impaired kidney function, including the filtration process. Celery (Apium graveolens L.) contains phenolic compounds that can prevent the decrease of kidney function. This study aims to determine the effect of celery ethanol extract on proteinuria in the UUO rat model. This study was an experimental study with a post-test only with control group design. A total of 25 rats were assigned to 5 groups. Group 1: sham control, 2: negative control (UUO), 3: UUO with 125 mg/kgBW celery ethanol extract, 4: UUO with 250 mg/kgBW celery ethanol extract, and 5: UUO with 500 mg/kgBW celery ethanol extract. The treatment of celery ethanol extract was given for 14 days. Urine samples were taken on the 15th day for examination of proteinuria. The results were analyzed by the Oneway ANOVA test and showed a significant difference between the control group and the treatment groups (p<0.05). A dose of 250mg/kgBW is the most effective dose of celery ethanol extract in this study.","PeriodicalId":255709,"journal":{"name":"Proceeding ICMA-SURE","volume":"646 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115830576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of main fungal disease from hydroponic melon in greenhouse 温室水培甜瓜主要真菌病害鉴定
Proceeding ICMA-SURE Pub Date : 2023-04-05 DOI: 10.20884/2.procicma.2023.2.1.7920
E. Sumarni, L. Soesanto, O. Herliana, N. W. A. Leana, Priswanto Priswanto, W. H. Purnomo, L. Zulkifli
{"title":"Identification of main fungal disease from hydroponic melon in greenhouse","authors":"E. Sumarni, L. Soesanto, O. Herliana, N. W. A. Leana, Priswanto Priswanto, W. H. Purnomo, L. Zulkifli","doi":"10.20884/2.procicma.2023.2.1.7920","DOIUrl":"https://doi.org/10.20884/2.procicma.2023.2.1.7920","url":null,"abstract":"Hydroponic melon is a method of cultivating melons and grown in a greenhouse. The melon cultivation has problems, namely plant diseases, even in a closed greenhouse. The melon plant disease caused huge losses in melon cultivation until the melon plants died. The purpose of this descriptive study was to identify the cause of the melon plant disease. Samples of sick melon plants were taken and grown on PDA media in the laboratory. The fungus that grows is identified based on the literature. The identification results showed that the main disease causing hydroponic melon plants in the greenhouse was the fungus Fusarium oxysporum f.sp. melonis. The fungi have crescent-shaped macroconidia with 1-3 septa, hyaline, thick walls, and form chlamydospores that are spherical in intercalary and hyaline.","PeriodicalId":255709,"journal":{"name":"Proceeding ICMA-SURE","volume":"317 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123684577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Water Recharge Variability Across Serayu Watershed Using Soil Water Assessment Tool (SWAT) 基于土壤水分评估工具(SWAT)的Serayu流域补水变化
Proceeding ICMA-SURE Pub Date : 2023-04-03 DOI: 10.20884/2.procicma.2023.2.1.8425
A. Hardanto, A. Mustofa, A. Ardiansyah
{"title":"Water Recharge Variability Across Serayu Watershed Using Soil Water Assessment Tool (SWAT)","authors":"A. Hardanto, A. Mustofa, A. Ardiansyah","doi":"10.20884/2.procicma.2023.2.1.8425","DOIUrl":"https://doi.org/10.20884/2.procicma.2023.2.1.8425","url":null,"abstract":"Climate change and land use have an influence on the process of groundwater replenishment. Serayu watershed is categorized as one of the critical watersheds in Indonesia due to groundwater conditions. One that affects groundwater is the ability to replenish groundwater. The purpose of the study was to predict groundwater replenishment in the Serayu watershed. The hydrological model approach (SWAT/Soil Water Assessment Tool) was applied. Spatial data is used in SWAT model analysis, namely: Elevation data, stream, soil types, land use land cover, and climate data. Data analysis using QGIS 3.18 and QSWATPlus tools. The model calibration uses cn2 values while NSE and R2 values are used to evaluate the model. Based on cn2 values, SWAT models show performance that can be used for model implementation. Model validation between before and after the calibration process is (R2) 0.86 while the NSE value is 0.66. When viewed from the water equilibrium ratio, the average water recharge value in the Serayu watershed is 49.5 mm.yr-1 from the total precipitation of 2473.6 mm.yr-1. The largest hydrological component occurs in evapotranspiration and surface runoff (1879.9 and 549.2 mm.yr-1, respectively). The high value of surface runoff indicates that the Serayu watershed needs attention regarding the ability of the land in the water recharge process","PeriodicalId":255709,"journal":{"name":"Proceeding ICMA-SURE","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114570801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Morphotectonic Study Based On The Mountain Sinuosity (SMF) Method in Kramat And Surrounding Area, Karangmoncol-Purbalingga District 基于山曲度(SMF)方法的克拉玛特及周边地区地貌构造研究
Proceeding ICMA-SURE Pub Date : 2023-03-24 DOI: 10.20884/2.procicma.2023.2.1.8331
A. Widagdo, R. Setijadi
{"title":"The Morphotectonic Study Based On The Mountain Sinuosity (SMF) Method in Kramat And Surrounding Area, Karangmoncol-Purbalingga District","authors":"A. Widagdo, R. Setijadi","doi":"10.20884/2.procicma.2023.2.1.8331","DOIUrl":"https://doi.org/10.20884/2.procicma.2023.2.1.8331","url":null,"abstract":"Abstract. The study area is in the Karangmoncol District and its surroundings, Purbalingga Regency, Central Java Province, Indonesia. The study was conducted to determine the level of tectonic activity using morphometric analysis in the slopes and valleys. The analysis carried out is Mountain Front Sinuosity (Smf). Geological mapping was carried out to determine the types of rocks in the research area. Smf measurements were carried out on each rock group. Smf measurement is done by selecting a certain elevation contour line facing the valley. Two points on the contour line are connected by a straight line. The value of Smf is calculated by dividing the length of the selected contour line (Lmf) by the length of the straight line of two points on the contour (Ls). The rock of the Halang Formation in the northern part of the study area which is old (Late Miocene) has a high Smf value (3.89 points) which indicates it is not tectonically active. The rock of the Kumbang Formation in the middle of the study area that is old (Late Miocene) has a moderate Smf value (2.67 points) which indicates it has a weak tectonic level. The rocks of the Kumbang Formation are composed of volcanic rocks such as lava, breccia and tuff that are dipped to the south. The young Tapak Formation rock (Pliocene) in the southern part of the study area has a high Smf (3.16 points) which indicates it is not tectonically active. Keywords: tectonic, morphometric, formation, Miocene, and rock.","PeriodicalId":255709,"journal":{"name":"Proceeding ICMA-SURE","volume":"222 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121566411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The External, Internal Factor and Ecosystem Services to Support Mangrove Rehabilitation Planning in North Coast of Jakarta 支持雅加达北海岸红树林恢复规划的外部、内部因素和生态系统服务
Proceeding ICMA-SURE Pub Date : 2023-02-14 DOI: 10.20884/2.procicma.2023.2.1.7783
E. Hilmi, Uus Usman, A. Iqbal
{"title":"The External, Internal Factor and Ecosystem Services to Support Mangrove Rehabilitation Planning in North Coast of Jakarta","authors":"E. Hilmi, Uus Usman, A. Iqbal","doi":"10.20884/2.procicma.2023.2.1.7783","DOIUrl":"https://doi.org/10.20884/2.procicma.2023.2.1.7783","url":null,"abstract":"The mangrove ecosystem on North Coast of Jakarta has many services and function both of ecology, social and economic function and services, including the tidal flooding reduction, land subsidence reduction, pollution reduction, ecotourism and others. However, the mangrove ecosystem in North Coast of Jakarta Mangroves are being badly damaged, so require effort and activity to rehabilitate. This research aimed to develop  rehabilitation strategy of mangrove ecosystem in North Coast of Jakarta to reduce coastal disaster and to support ecosystem services. The methods of this research used IFAS, EFAS analysis and Buchard analysis. The results showed that the ecosystem services of mangrove ecosystem were are ecotourism, conservation, wildlife reserves, reduction of tidal flooding, abrasion and accretion, reduction of intrusion, reduction of land subsidence, economic income, fishing and fishpond activities, pond activities, and social benefits. The strategy of mangrove rehabilitation was weakness-opportunity strategy (minimalizing weakness,  taking advantage of opportunities, and avoid threats. The strategies of mangrove rehabilitation plaaning were mangrove rehabilitation, mangrove revitalization, support creative economy, developing greenbelt, increasing human resources and developing blue carbon","PeriodicalId":255709,"journal":{"name":"Proceeding ICMA-SURE","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124405787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fictitious-Positive Decision Dispute Resolution at PTUN For the Achieving Unity of Proceedings and Legal Certainty PTUN的虚正判决纠纷解决实现程序与法律确定性的统一
Proceeding ICMA-SURE Pub Date : 2023-02-13 DOI: 10.20884/2.procicma.2023.2.1.7750
Weda Kupita, R. Ardhanariswari
{"title":"Fictitious-Positive Decision Dispute Resolution at PTUN For the Achieving Unity of Proceedings and Legal Certainty","authors":"Weda Kupita, R. Ardhanariswari","doi":"10.20884/2.procicma.2023.2.1.7750","DOIUrl":"https://doi.org/10.20884/2.procicma.2023.2.1.7750","url":null,"abstract":"The Administrative Court (PTUN) has the authority to adjudicate ordinary state administrative disputes, namely disputes resulting from the issuance of written decisions by government officials, these disputes are classified as \"ordinary state administrative disputes\". The Administrative Court is also authorized to adjudicate \"fictitious-positive decision disputes\", namely disputes caused by legal events in the form of silence by government officials not issuing the requested decision, then the application is considered legally granted. Plaintiff (person or civil legal entity), before filing a lawsuit to the Administrative Court, it must first take an \" Administrative Appeal \", it is determined if the authorized official does not respond (acts in silence) to Administrative Appeal, then \"Administrative Appeal are deemed granted\". Then there will be a juridical problem: \"whether the dispute will be tried by PTUN as\" Ordinary State Administrative Dispute \"or as\" Fictitious-Positive Decision Dispute? \".  The approach method used is Normative Juridical, qualitative normative analysis method, and grammatical and systematic interpretation method. Based on normative studies, it is not yet clear whether the legal event will become an \"Ordinary TUN Dispute\" or a \"Fictitious-Positive Decision Dispute\", because it cannot be classified into the two types of disputes. Certainty is needed, because the procedural law are very different between the two types of disputes. The Article 77 and Article 78 of the UU-AP need to be amended by adding the provisions in the next paragraph which stipulates: \"if the applicant for an administrative objection or appeal wishes to submit to the Administrative Court, then the TUN dispute is resolved in the Ordinary Procedure or is resolved as a fictitious decision dispute- positive\". It is very important to emphasize in the provisions of the article in order to realize the unity of the proceedings and legal certainty","PeriodicalId":255709,"journal":{"name":"Proceeding ICMA-SURE","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125196803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
THE URGENCY OF PARALEGAL POSITION IN LAW ENFORCEMENT IN INDONESIA THROUGH THE PROVISION OF LEGAL AID 通过提供法律援助,律师助理在印尼执法中的地位的紧迫性
Proceeding ICMA-SURE Pub Date : 2023-02-10 DOI: 10.20884/2.procicma.2023.2.1.7749
Nurani Ajeng Tri Utami, Hibnu Nugroho
{"title":"THE URGENCY OF PARALEGAL POSITION IN LAW ENFORCEMENT IN INDONESIA THROUGH THE PROVISION OF LEGAL AID","authors":"Nurani Ajeng Tri Utami, Hibnu Nugroho","doi":"10.20884/2.procicma.2023.2.1.7749","DOIUrl":"https://doi.org/10.20884/2.procicma.2023.2.1.7749","url":null,"abstract":"Legal aid distribution at the moment does not yet reach the whole Indonesian people because existing limitations for the implementation of the law so that paralegal is required to increase the range of legal aid. Considering the importance of that, it is needed to legitimation for a paralegal position in enforcement law for optimizing the role of the paralegal. The aim of this study is to knowing the importance and legitimacy of paralegal position in regulations legislation in Indonesia and to knowing optimization of the position and role of paralegals in enforcement law through legal aid. The method research used is juridical normative with approach statute, analysis, and concepts. Research results show that reason that is a consequence of the rule of law adopted by Indonesia and is a right constitutional as well as a protection right basic as equality before the law, setting more carry on of legal aid regulation, as aspect obligations and state responsibilities in giving legal aid, guarantee certainty for paralegal law in operating their duties, and as the effort for creating order in organizing giver legal aid especially paralegals. Optimization of paralegals’ position in the enforcement of Indonesian law in giving legal aid could be conducted through increasing paralegal competence with follow the training, networking, and cadre of paralegals from start district level, regional until lowest in the village, Skill advocate Public in the form of defense and support to the community, and giving law protection for paralegals in operating their job in giving legal aid. \u0000 ","PeriodicalId":255709,"journal":{"name":"Proceeding ICMA-SURE","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124727133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Immoral Offense Eligibility as Criminal Acts Objects for Restorative Justice Filing 不道德犯罪作为刑事行为资格的追诉对象
Proceeding ICMA-SURE Pub Date : 2023-02-07 DOI: 10.20884/2.procicma.2023.2.1.7766
Dwiki Oktobrian, Guntur Pembayun Putro, Aura Salsabila Ayodya Swastiko
{"title":"Immoral Offense Eligibility as Criminal Acts Objects for Restorative Justice Filing","authors":"Dwiki Oktobrian, Guntur Pembayun Putro, Aura Salsabila Ayodya Swastiko","doi":"10.20884/2.procicma.2023.2.1.7766","DOIUrl":"https://doi.org/10.20884/2.procicma.2023.2.1.7766","url":null,"abstract":"Restorative Justice has experienced a new phase in Indonesia since 2020. Since that year, the Police and the Prosecutor’s Office have been competing to formulate internal regulations as a form of deadlock in revising the Indonesian Criminal Code Procedure (KUHAP), one of the new substances of which is the idea of Restorative Justice. Restorative Justice allows a case to not proceed to the court based on a peace agreement between the victim and the offender to create the restoration for the victims and social harmonization. Recently, there have been cases in Indonesia regarding the use of Restorative Justice in immoral cases, such as rape, that is then reconciled by marrying the victim and the offender. Such marriage in no way will bring happiness or restore the victim’s condition but adds to the suffering of the victim and further destroys social harmony. This phenomenon is analysed with a normative approach which shows that the factors for the emergence of daunting peace under the pretext of Restorative Justice arise from the regulations dissimilarity between the Police and the Prosecutor’s Office; only in the Prosecutor’s Office regulation, immoral cases are cases that are excluded as objects of cases in Restorative Justice. Police regulations improvement must be carried out immediately so that the practice of marrying the victims with their offenders in immoral cases can be completely terminated from the start. Even a peace process proposal for immoral crime cases should not need to be facilitated because of the heavy impact felt by the victim or their family.","PeriodicalId":255709,"journal":{"name":"Proceeding ICMA-SURE","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121708577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The primary culture and subculture of kidney-derived cells of the bony lip barb fish (Osteochilus vittatus) using different concentrations of serum 不同浓度血清对骨唇刺鱼(Osteochilus vittatus)肾源细胞的原代培养和传代培养
Proceeding ICMA-SURE Pub Date : 2023-02-06 DOI: 10.20884/2.procicma.2023.2.1.7818
Alif Arfa Maulida Firdauzi, Gratiana Ekaningsih Wijayanti, A. N. Habibah
{"title":"The primary culture and subculture of kidney-derived cells of the bony lip barb fish (Osteochilus vittatus) using different concentrations of serum","authors":"Alif Arfa Maulida Firdauzi, Gratiana Ekaningsih Wijayanti, A. N. Habibah","doi":"10.20884/2.procicma.2023.2.1.7818","DOIUrl":"https://doi.org/10.20884/2.procicma.2023.2.1.7818","url":null,"abstract":"Cell and tissue culture have advanced and developed in a short time yet continuous improvement needs to be conducted for better results. This research was conducted to obtain suitable culture conditions of kidney-derived cell culture from bony lip barb kidney (Osteochilus vittatus). The development of primary cell culture from the kidney fragments was performed in a cell culture medium composed of DMEM supplemented with various concentrations (0%, 5%, 10%, and 15%) of Fetal Bovine Serum, Penicillin-Streptomycin, and 2Mm L-Glutamine, and incubated at 29°C with 0.8% CO2. The results showed that the primary cell culture achieved confluence on day four and subsequently subcultured. The addition of 10% serum increased cell density and cell survival. The cell morphological evaluation using a phase-contrast microscope showed two dominant cell types for both primary and subcultures, i.e. erythrocytes and small-rounded cells. The highest cell yield was obtained with the addition of 10% serum concentration with Population Doubling Time (PDT) and Population Doubling Level (PDL) for 69 h 23 min and 0.47 times, respectively. Statistical analysis indicated that serum addition significantly increased cell density of the primary culture (p<0.05). A better proportion of serum supplementation to culturing success is essential to establish an auspicious cell line.","PeriodicalId":255709,"journal":{"name":"Proceeding ICMA-SURE","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132590280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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