The Journal of Agriculture and Development最新文献_第3页

Modeling the stem taper functions for Acacia hybrid in Dong Nai province 同奈地区金合欢杂交种茎尖函数的建模

The Journal of Agriculture and Development Pub Date : 2023-08-25 DOI: 10.52997/jad.2.04.2023

Them V. Nguyen

引用次数: 0

Selection of Arabidopsis transformants containing AtZAT12 含AtZAT12的拟南芥转化子的筛选

The Journal of Agriculture and Development Pub Date : 2023-08-25 DOI: 10.52997/jad.4.04.2023

C. T. T. Le, N. T. Pham

{"title":"Selection of Arabidopsis transformants containing AtZAT12","authors":"C. T. T. Le, N. T. Pham","doi":"10.52997/jad.4.04.2023","DOIUrl":"https://doi.org/10.52997/jad.4.04.2023","url":null,"abstract":"This study aimed to select Arabidopsis seeds after transformation with the AtZAT12 gene via Agrobacterium tumerfaciens. ZAT12 is a transcription factor that inhibits other transcription factors FIT through the EAR motif. FIT itself is a central transcription factor that controls Fe uptake under Fe-deficient conditions. However, if Fe is absorbed excessively, it will produce reactive oxygen species that could damage cells. That is the reason why AtZAT12 transcription is elevated and FIT transcription is inhibited under Fe deficiency for 10 days. To investigate the function, the AtZAT12 gene was inserted into the pMDC107 vector for transformation into Arabidopsis. The T0 Arabidopsis seeds obtained after floral dip transformation were placed on 1% MS agar supplemented with 15 μg/mL Hygromycin B. Beforehand, the T0 seeds were sterilized and kept in the dark for stratification. Subsequently, the seed plates were subjected to a regime of 6 h of light, 48 h of dark and 24 h of light (3.25 d). The hygromycin B-resistant seedlings had long hypocotyls (~ 1.0 cm), while the non-resistant seedlings had short (~ 0.3 cm) hypocotyls. This method took only 3.25 days to identify transgenic Arabidopsis seedlings. After that, positive transgene lines were examined by PCR method for AtZAT12, and the expression of AtZAT12 was observed under microscope.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132576458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of pyometra in dogs in Ho Chi Minh City, Vietnam 越南胡志明市犬脓脓调查

The Journal of Agriculture and Development Pub Date : 2023-08-25 DOI: 10.52997/jad.6.04.2023

H. T. Pham, Thuong-Thuong Nguyen

{"title":"Investigation of pyometra in dogs in Ho Chi Minh City, Vietnam","authors":"H. T. Pham, Thuong-Thuong Nguyen","doi":"10.52997/jad.6.04.2023","DOIUrl":"https://doi.org/10.52997/jad.6.04.2023","url":null,"abstract":"The objectives of this study were to evaluate the status of pyometra in dogs and effects of treatment, and to isolate bacteria from the inflammatory fluid of pyometra in dogs. The study was carried out from September 2022 to February 2023 at the Animal Health Laboratory and Treatment Division, Sub-Department of Animal Health and Husbandry of Ho Chi Minh City. Out of total 1,715 bitches, there were 81 cases of pyometra (4.43%). The results showed that dogs aged 5 - 7 years (35.80%) had the highest rate of pyometra, followed by dogs aged 2 - 4 years (20.99%), and the lowest rate with dogs < 2 years old (3.70%). Local breeds had a lower pyometra rate (19.75%) compared to foreign breeds (80.25%) (P < 0.05). Dogs kept in cages accounted for 58.02% of the pyometra rate, which was higher than the rate of free-roaming dogs at 41.98% (P < 0.05). Non-reproductive dogs had the highest rate of this disease (64.20%), followed by primiparous dogs (25.93%) and the lowest rate was recorded in dogs with parity 5 (1.23%) (P < 0.05). Common clinical symptoms included moodiness/proneness, vaginal discharge, abdominal distension/pain to touch, excessive drinking/thirst, loss of appetite, dehydration, fever, weight loss, polyuria and vomiting, which were important for the diagnosis of pyometra. Streptococcus was a bacterial group found in uterine fluid samples of pyometra by isolation and culture method. The treatment method chosen by the owners for pyometra in dogs in Ho Chi Minh City was surgical intervention and the effect of successful treatment was very high (90.12%).","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134245262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and biological characteristics of Galleria mellonella L. (Lepidoptera: Pyralidae) reared on different artificial diets 不同人工饲料饲养的黄颡鱼蛾(鳞翅目:蚜蝇科)形态及生物学特性

The Journal of Agriculture and Development Pub Date : 2023-08-25 DOI: 10.52997/jad.1.04.2023

H. K. Le, Dong T. H. Tran

引用次数: 0

Isolation and genetic analysis of Tembusu virus in ducks 鸭坦布苏病毒的分离及遗传分析

The Journal of Agriculture and Development Pub Date : 2023-08-25 DOI: 10.52997/jad.5.04.2023

Dat M. Truong, H. Hoang

引用次数: 0

Efficiency of intensive tilapia culture in earthen ponds applied biofloc technology (BFT), probiotics and off-flavor control methods 土池罗非鱼集约化养殖应用生物絮团技术(BFT)、益生菌和异味控制方法的效果研究

The Journal of Agriculture and Development Pub Date : 2023-05-24 DOI: 10.52997/jad.2.03.2023

Loan P. Phan, Tuat V. Nguyen

{"title":"Efficiency of intensive tilapia culture in earthen ponds applied biofloc technology (BFT), probiotics and off-flavor control methods","authors":"Loan P. Phan, Tuat V. Nguyen","doi":"10.52997/jad.2.03.2023","DOIUrl":"https://doi.org/10.52997/jad.2.03.2023","url":null,"abstract":"The aim of this study was to assess the efficiency of Nile and red tilapia cultured in intensive systems applied a combination of biofloc technology (BFT) and probiotics in earthen ponds with different methods to control the off-flavor problem. A growing-out experiment was conducted for 150 days in which both Nile tilapia (5.88 ± 0.59 g) and red tilapia (9.72 ± 0.72 g) fingerlings were randomly allotted into three replicates of earthen ponds which were subdivided into four treatment groups, including: off-flavor controlled by algae management in Nile tilapia ponds (NT - AM) and red tilapia ponds (RT - AM), and off-flavor controlled by active water exchange in Nile tilapia ponds (NT - WE) and red tilapia ponds (RT - WE). The results showed that water quality was maintained in suitable ranges for fish growth. There were no significant differences in growth performance (final weight, daily weight gain and specific growth rate) among all treatments (P < 0.05). Feed conversion ratio of Nile tilapia (1.28 - 1.31) was significantly lower than that of red tilapia (1.35 - 1.37) (P < 0.05). The survival ratio (about 80%) and extrapolated yield (21.50 tons/ha in 150 days) of fish was similar and high for both Nile and red tilapia. The quality of the harvested fish in term of condition factor, size even and off-flavor intensity was also excellent. The economic efficiency of red tilapia farming in this system was higher as compared to cage systems. This study clearly demonstrated that the technique of combined BFT and probiotic application in earthen ponds could contribute to the sustainable development of tilapia production in Vietnam by reducing production cost, saving water resource and avoiding environmental pollution.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128832577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the database on land use planning in Vung Tau city, Ba Ria - Vung Tau province 改进巴里亚省头市土地利用规划数据库

The Journal of Agriculture and Development Pub Date : 2023-05-24 DOI: 10.52997/jad.6.03.2023

Linh D. Truong

{"title":"Improving the database on land use planning in Vung Tau city, Ba Ria - Vung Tau province","authors":"Linh D. Truong","doi":"10.52997/jad.6.03.2023","DOIUrl":"https://doi.org/10.52997/jad.6.03.2023","url":null,"abstract":"Vung Tau city is an area where management of land use planning (LUP) and urban planning is quite complicated and has been successful in building LUP database. However, this database still has limitations that must be addressed, including (1) lack of data layer on current land use for building LUP database, (2) incompleted LUP attribute database, (3) lack of metadata, (4) no connection between LUP database and digital legal materials and (5) no overlay cadastral database to data layer of current land use and LUP database. To solve these problems, this research used many methods such as: documents and data collection, inheritance, expert interview, data processing and analysis, mapping, GIS application, and IT applications. The results of this study showed that the structure of cadastral database of 8 wards was succesfully converted from existing structure (according to Circular 17/2010/TT-BTNMT) to the standard structure (according to Circular 75/2015/TT-BTNMT). The 2019 land inventory data was standardized and data layer of current land use for whole city was built. In addition, the LUP database (period 2010 - 2020) for Vung Tau was improved, including: supplementing attribute information, completing metadata, connecting LUP database with relevant digital legal records, overlaying cadastral database with LUP database and data layer of current land use. Briefly, our results could be a foundation to help Vung Tau manage land sustainably, contribute to successfully building national land database and meet the exploitation requirements of LUP information in accordance with the orientation of Ministry of Natural Resources and Environment for study area.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121520393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of loop-mediated isothermal amplification to detect Salmonella spp. in egg samples 环介导等温扩增技术在鸡蛋沙门氏菌检测中的应用

The Journal of Agriculture and Development Pub Date : 2023-05-24 DOI: 10.52997/jad.1.03.2023

Kha T. Tran, T. T. Nguyen

{"title":"Application of loop-mediated isothermal amplification to detect Salmonella spp. in egg samples","authors":"Kha T. Tran, T. T. Nguyen","doi":"10.52997/jad.1.03.2023","DOIUrl":"https://doi.org/10.52997/jad.1.03.2023","url":null,"abstract":"The objective of the study was to evaluate the use of loop-mediated isothermal amplification (LAMP) technique for detection of Salmonella spp. in chicken eggs. Eight Salmonella strains and 26 non-Salmonella enteric strains were used to verify the specificity of the LAMP assay for detection of Salmonella. The Sal4 primer set was used to specifically amplify the target sequence on the invA gene of Salmonella and the hydroxyl naphthol blue was used as the indicator to read the reaction results. Blank egg samples were spiked with serial 10-fold diluted Salmonella suspensions to determine the limit of detection at 50% (LOD50) of the assay. Egg samples collected from multiple locations in Ho Chi Minh City were tested for the presence of Salmonella using both LAMP method and the traditional culture method to compare the effectiveness of these two methods. Among 34 bacterial strains tested by LAMP assay, false-positive or false-negative results were not observed. The assays of spiked egg samples showed that the LOD50 of the LAMP method was less than 2 CFU/25 g sample. These results indicated a high specificity and sensitivity of the LAMP assay in detection of Salmonella in egg samples, even with those of low levels of contamination. Upon testing collected egg samples (n = 42), the LAMP assay produced the same results of Salmonella detection as the culture method. Salmonella was detected in 4 out of 42 samples. This study showed that the LAMP method is highly effective and would be suitable for use in detection of Salmonella in egg samples. The assay has several advantages such as saving time and labor compared to traditional culture methods.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127621457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characteristics of Pseudomonas fluorescens to inhibit Phytophthora palmivora causing rot disease in durian 荧光假单胞菌的分离及对榴莲腐病棕榈疫霉的抑制作用

The Journal of Agriculture and Development Pub Date : 2023-05-24 DOI: 10.52997/jad.4.03.2023

Van-Tuan Tran, H. Nguyen, H. T. Nguyen, Don D. Le

{"title":"Isolation and characteristics of Pseudomonas fluorescens to inhibit Phytophthora palmivora causing rot disease in durian","authors":"Van-Tuan Tran, H. Nguyen, H. T. Nguyen, Don D. Le","doi":"10.52997/jad.4.03.2023","DOIUrl":"https://doi.org/10.52997/jad.4.03.2023","url":null,"abstract":"Plant growth promoting Rhizobacteria (PGPR) have long been selected as biocontrol agents for plants. The PGPR are beneficial bacteria that live in plant roots and enhance plant growth by various of mechanisms. The PGPR have many species of the bacterial genus Pseudomonas, in which the Pseudomonas fluorescens strains is suitable for application as a biocontrol agent due to its abundance in natural soil and plant root systems. Pseudomonas fluorescens strains have important characteristics such as the ability to adhere to soil particles and to rhizomes, the ability to synthesize antibiotics, and to produce hydrolytic enzymes. Furthermore, P. fluorescens strains also possess plant growth promoting characteristics such as proteolysis, phosphate solubilization, iron chelation, and phytohormone production. Durian is a crop that has been expanded in Southeast Asian countries but the rate of durians infected with root, stem and fruit rot caused by P. palmivora is quite large. In this study, five native P. fluorescens isolates were isolated from 70 soil and rhizome samples collected from 10 durian growing provinces and evaluated for their ability to antagonize P. palmivora in petri plates. These isolates were gram negative, small, single isolated rods without sporulation when observed under microscope. All of them tested positive for catalase test, oxidase test, starch hydorolysis, gelatine liquefaction, H2S, citrate utilization and negative with indole, Voges-proskauers, methyl red. All of 5 native P. fluorescens isolates were capable of phosphate solubilizing activity, N2 fixation, siderophore and IAA production. The study showed that P. fluorescens P. fDN strain was able to inhibit P. palmivora causing rot disease in durian with 51.85% inhibition of radial growth.","PeriodicalId":250563,"journal":{"name":"The Journal of Agriculture and Development","volume":"220 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130692943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water desalination of Chlorella vulgaris 小球藻的海水淡化

The Journal of Agriculture and Development Pub Date : 2023-05-24 DOI: 10.52997/jad.5.03.2023

Viet B. Nguyen, H. T. Nguyen, G. Ho, H. T. Nguyen, Suong T. T. Nguyen, Dao N. Duy, Sang M. Nguyen

引用次数: 0