Thrombosis & Haemostasis: Research最新文献

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Circular RNA Circrna_0072387 Promotes Hepatoblastoma Progression by Regulating Hsa-Mir-490/Histone Deacetylase 2 Axis 环状RNA Circrna_0072387通过调节Hsa-Mir-490/组蛋白去乙酰化酶2轴促进肝母细胞瘤进展

Thrombosis & Haemostasis: Research Pub Date : 2022-03-25 DOI: 10.26420/thrombhaemostres.2022.1074

G. H, Y. R., L. J, L. L, Sun X, X. C.

{"title":"Circular RNA Circrna_0072387 Promotes Hepatoblastoma Progression by Regulating Hsa-Mir-490/Histone Deacetylase 2 Axis","authors":"G. H, Y. R., L. J, L. L, Sun X, X. C.","doi":"10.26420/thrombhaemostres.2022.1074","DOIUrl":"https://doi.org/10.26420/thrombhaemostres.2022.1074","url":null,"abstract":"Circular RNAs have been reported to be associated with the occurrence and progress of various cancers. However, its biological roles in hepatoblastoma (HB) remain undefined. This study intended to explain a possible mechanism of hsa_circ_0072387 (circ72387) in hepatoblastoma progression. The expression of circ72387, hsa-miR-490 (miR490) and histone deacetylase 2 (HDAC2) in HB tissues and cell lines (HepG2/Huh6) were determined by quantitative realtime polymerase chain reaction (qPCR) or Western blot. Cell Counting Kit-8 (CCK-8) and colony formation assay were used to detect the proliferation of HB cell lines. The interaction between miR490 with circ72387 was predicted by CircInteractome and was verified by Dual-Luciferase reporter assay while the interaction between miR490 with HDAC2 was predicted by Targetscan and was verified by western blotting assay. Results showed that circ72387 was upregulated in both HB tissues and cells, and miR490 was downregulated in HB cells. Furthermore, the silence of circ72387 suppressed the proliferation of hepatoblastoma cells. Circ72387 promoted the proliferation of HB cells through competitively binding to HDAC2 against miR490 acting as a Sponge of miR490 in HB. Circ72387 played an essential role in HB development through the circ72387-miR490-HDAC2 regulatory axis, which may pave the way for potential diagnostic markers and therapeutic targets research in HB.","PeriodicalId":246370,"journal":{"name":"Thrombosis & Haemostasis: Research","volume":"132 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129020271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benefits of Prolonged Anticoagulant Treatment in a Series of Cerebral Venous Thrombosis in Pediatric Patients 延长抗凝治疗对儿童脑静脉血栓形成的益处

Thrombosis & Haemostasis: Research Pub Date : 2022-02-17 DOI: 10.26420/thrombhaemostres.2022.1073

Berrueco R, Faura A, R. A, M. M, S. M, G. E.

{"title":"Benefits of Prolonged Anticoagulant Treatment in a Series of Cerebral Venous Thrombosis in Pediatric Patients","authors":"Berrueco R, Faura A, R. A, M. M, S. M, G. E.","doi":"10.26420/thrombhaemostres.2022.1073","DOIUrl":"https://doi.org/10.26420/thrombhaemostres.2022.1073","url":null,"abstract":"Background: Cerebral sinus venous thrombosis (CSVT) is a rare complication in children, usually secondary to an underlying condition. Recent guidelines recommend treating all pediatric patients with CSVT, but also suggest that treatment longer than three months should not be justified in provoked cases in which the risk factor has disappeared. Methods: Retrospective observational study analyzing data of CSVT in children (1-18 years-old) admitted in a single hospital. Demographic data, clinical presentation, etiology, extension, treatment and outcome were evaluated. Results: Thirty-one patients had a confirmed CSVT. Sixteen were girls, mean age was 7.3 years (SD 4.39). Sigmoid sinus (n=17) and transverse sinus (n=14) were the most frequent sinuses affected. Multiple sinus thrombosis was found in 20 patients (64.5%). Underlying condition was described in 29 cases: infectious disease (n=20), major head trauma (n=4), chemotherapy (n=3), arteriovenous malformation (n=2). More frequent symptoms were: headache (n= 15), decreased level of consciousness (n=5), seizures (n=3), focal neurological deficit (n=2), papilledema (n=2), diplopia (n=2), vision impairment (n=1), and coma (n=4). All patients received anticoagulant treatment. Complete vein recanalization after 3 months was achieved in 53.3% of the cases. Treatment was prolonged up to 6 months in those patients with partial or no response (n=14). Seven of them improved radiological findings. Complete and partial vein recanalization were reported in 73.3% and 20% of the patients. Conclusion: Anticoagulation was useful to achieve complete vein recanalization in this series. Extending anticoagulation up to 6 months could be useful to improve outcome of pediatric patients with CSVT in selected cases.","PeriodicalId":246370,"journal":{"name":"Thrombosis & Haemostasis: Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116218135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mitochondrial Superoxide is the Key for Promoting Cell Migration in Wound Healing 线粒体超氧化物是促进伤口愈合过程中细胞迁移的关键

Thrombosis & Haemostasis: Research Pub Date : 2022-02-03 DOI: 10.26420/thrombhaemostres.2022.1072

Ping Weidong, Wang Xin, Wang Xiaowei, Li Fei, H. Chunyan, Zhao Qiming

{"title":"Mitochondrial Superoxide is the Key for Promoting Cell Migration in Wound Healing","authors":"Ping Weidong, Wang Xin, Wang Xiaowei, Li Fei, H. Chunyan, Zhao Qiming","doi":"10.26420/thrombhaemostres.2022.1072","DOIUrl":"https://doi.org/10.26420/thrombhaemostres.2022.1072","url":null,"abstract":"Introduction: The focus of current study was to investigate the role of mitochondrial superoxide for promoting cell migration in healing responses. Methods: To simulate the skin wound healing process, scratch wound assay in A549 cells was utilized. Subsequently, mitochondrial superoxide and intracellular calcium flux (Ca2+) were measured by MitoSox red reagent and Fluo-4 staining. Paraquat (PQ), N-acetyl cysteine (NAC), superoxide dismutase (SOD) mimics (EUK-134), free radical scavenge (MCI-186), and diphenyliodonium (DPI) were added into medium to evaluate the influences of reactive oxygen species (ROS) and superoxide on cell migration. Results: Compared with before wound (BW), mitochondrial superoxide increased time-dependent manner. Correspondingly, Ca2+ transients was rapidly elevated after scratch wound and gradually declined toward an steady level, as evidenced by Fluo-4 fluorescence intensity. Scratch wound assay displayed that cell migration in PQ 0.1 mM group was stronger than that of Control group, whilst it was restrained after administration of 10 mM NAC. Additionally, cell migration in EUK-134 and MCI-186 group was conspicuously lower than that of Control group and similar with the degree of inhibiting role of DPI, indicating that mitochondrial superoxide served the crux influence on promoting cell migration after scratch. Conclusion: ROS and superoxide production during scratch wound facilitated cell migration. Importantly, mitochondrial superoxide played the positive effects on healing response.","PeriodicalId":246370,"journal":{"name":"Thrombosis & Haemostasis: Research","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127038575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

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Thrombosis &amp; Haemostasis: Research最新文献

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