Pathobiology : journal of immunopathology, molecular and cellular biology最新文献_第4页

Thyroid Tubercle of Zuckerkandl May Not Arise from the Ultimobranchial Body: Results from Histological Analysis. 朱克曼氏甲状腺结节可能不是起源于鳃末体:组织学分析结果。

IF 5

Pathobiology : journal of immunopathology, molecular and cellular biology Pub Date : 2020-01-01 Epub Date: 2020-04-06 DOI: 10.1159/000506231

Miyoko Higuchi, Mitsuyoshi Hirokawa, Ayana Suzuki, Hiroo Masuoka, Akira Miyauchi

{"title":"Thyroid Tubercle of Zuckerkandl May Not Arise from the Ultimobranchial Body: Results from Histological Analysis.","authors":"Miyoko Higuchi, Mitsuyoshi Hirokawa, Ayana Suzuki, Hiroo Masuoka, Akira Miyauchi","doi":"10.1159/000506231","DOIUrl":"https://doi.org/10.1159/000506231","url":null,"abstract":"Thyroid tubercle of Zuckerkandl (TZ) is a nodule arising from the posterolateral thyroid, considered to be a remnant of the ultimobranchial body (UB). Considering that C cells and solid cell nests also arise from the UB, we hypothesized that these would be present in the TZ. We examined the presence of C cells and solid cell nests in the TZ using the histological analyses of 21 patients with grade 2 or 3 TZs following Pelizzo's grading system. Out of 21 TZs, 19 (90.5%) were located in the right lobe of the thyroid. Microscopically, solid cell nests were found within the TZ in 1 case (4.8%), and within the main thyroid tissues in 3 cases (14.3%). Calcitonin-positive C cells were scattered within the TZ in 1 case (4.8%), and within the main thyroid tissue in 15 cases (71.4%). The distribution of C cells within the main thyroid tissue was denser than that within the TZ. The above-mentioned results indicated the lack of C cells and solid cell nests in the TZ. Although the TZ may have an embryological origin different from that of ordinary thyroid tissue, it is unlikely that the remnants of the UB are involved in the formation of the TZ.","PeriodicalId":244631,"journal":{"name":"Pathobiology : journal of immunopathology, molecular and cellular biology","volume":" ","pages":"193-197"},"PeriodicalIF":5.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000506231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37806303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular Profile of Gastrointestinal Stromal Tumors in Sixty-Eight Patients from a Single Swiss Institution. 来自一家瑞士机构的68名胃肠道间质瘤患者的分子特征。

IF 5

Pathobiology : journal of immunopathology, molecular and cellular biology Pub Date : 2020-01-01 Epub Date: 2020-02-20 DOI: 10.1159/000505407

Simon Haefliger, Katharina Marston, Darius Juskevicius, Nathalie Meyer-Schaller, Anja Forster, Stefan Nicolet, Paul Komminoth, Edouard Stauffer, Gieri Cathomas, Sylvia Hoeller, Luigi Tornillo, Stefan Dirnhofer, Luigi M Terracciano, Michel Bihl, Matthias S Matter

{"title":"Molecular Profile of Gastrointestinal Stromal Tumors in Sixty-Eight Patients from a Single Swiss Institution.","authors":"Simon Haefliger, Katharina Marston, Darius Juskevicius, Nathalie Meyer-Schaller, Anja Forster, Stefan Nicolet, Paul Komminoth, Edouard Stauffer, Gieri Cathomas, Sylvia Hoeller, Luigi Tornillo, Stefan Dirnhofer, Luigi M Terracciano, Michel Bihl, Matthias S Matter","doi":"10.1159/000505407","DOIUrl":"https://doi.org/10.1159/000505407","url":null,"abstract":"Introduction: Gastrointestinal stromal tumor (GIST) is the most common mesenchymal neoplasm of the gastrointestinal tract. It has distinct molecular features and primarily affects the KIT and PDGFRA genes.Objective: We wanted to assess the molecular profile of 68 GIST patients who were sequenced consecutively between 2014 and 2019 at our institute of pathology.Methods: Our cohort comprised 60 primary and 8 metastatic GIST patients; 43 and 57% of the cases, respectively, were analyzed by Sanger sequencing or next-generation sequencing (NGS).Results: Of the 60 primary GIST patients, 47 (78%) showed a KIT mutation; 2 cases showed a double KIT mutation, and 1 of these was a therapy-naive GIST. Nine (15%) patients harbored a PDGFRA mutation, 2 (3%) had a BRAF mutation, 1 (2%) had a PIK3CA mutation, and 1 (2%) did not show any mutation. One BRAF and the PIK3CA mutation have not been described in GIST before. All metastatic GIST harbored exclusively KIT mutations.Conclusion: A retrospective analysis of GIST sequenced at our institute revealed incidences of KIT and PDGFRA mutations comparable to those in other cohorts from Europe. Interestingly, we found 2 previously undescribed mutations in the BRAF and PIK3CA genes as well as 1 treatment-naive case with a double KIT mutation in exon 11.","PeriodicalId":244631,"journal":{"name":"Pathobiology : journal of immunopathology, molecular and cellular biology","volume":" ","pages":"171-178"},"PeriodicalIF":5.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000505407","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37660325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Phosphorylation of STAT3 in Undifferentiated Pleomorphic Sarcoma Is Correlated with a Favorable Prognosis. 未分化多形性肉瘤中STAT3磷酸化与良好预后相关

IF 5

Pathobiology : journal of immunopathology, molecular and cellular biology Pub Date : 2017-01-01 Epub Date: 2016-09-22 DOI: 10.1159/000448524

Hirofumi Bekki, Kenichi Kohashi, Yuichi Yamada, Kunio Iura, Takeaki Ishii, Akira Maekawa, Hiroshi Otsuka, Hidetaka Yamamoto, Michiyuki Hakozaki, Kazuki Nabeshima, Yukihide Iwamoto, Yoshinao Oda

{"title":"Phosphorylation of STAT3 in Undifferentiated Pleomorphic Sarcoma Is Correlated with a Favorable Prognosis.","authors":"Hirofumi Bekki, Kenichi Kohashi, Yuichi Yamada, Kunio Iura, Takeaki Ishii, Akira Maekawa, Hiroshi Otsuka, Hidetaka Yamamoto, Michiyuki Hakozaki, Kazuki Nabeshima, Yukihide Iwamoto, Yoshinao Oda","doi":"10.1159/000448524","DOIUrl":"https://doi.org/10.1159/000448524","url":null,"abstract":"Objective: The Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway plays a role in various biological processes. Phosphorylated STAT3 (p-STAT3) functions as a transcriptional factor, and suppressor of cytokine signaling 3 (SOCS3) is a potential inhibitor of STAT3. Here, we analyzed the status of the JAK-STAT pathway in undifferentiated pleomorphic sarcoma (UPS).Methods: We performed immunohistochemistry in 79 samples of UPS and Western blotting in 10 frozen samples. We also examined alterations in protein expression in the JAK-STAT pathway after the inhibition of phosphorylated Akt (p-Akt) or extracellular signal-regulated kinase (p-Erk) in vitro.Results: Immunohistochemically, p-STAT3 and SOCS3 were positive in 59.7 and 55.8%, respectively. Positivity for p-STAT3 was significantly correlated with a better prognosis (p = 0.0006) and negatively with SOCS3 expression (p = 0.0223). Positivity for SOCS3 was significantly correlated with a worse prognosis (p = 0.0001). Western blotting analysis revealed that p-STAT3 expression was lower in tumor than in normal tissue. In vitro results demonstrated that there was no detectable change in the expression of p-STAT3 regardless of the status of p-Akt or p-Erk.Conclusion: p-STAT3 may be a useful prognostic factor for UPS.","PeriodicalId":244631,"journal":{"name":"Pathobiology : journal of immunopathology, molecular and cellular biology","volume":" ","pages":"161-169"},"PeriodicalIF":5.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000448524","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39979789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Altered HLA class I and HLA-G expression is associated with IL-10 expression in patients with cervical cancer. 宫颈癌患者HLA I类和HLA- g表达改变与IL-10表达相关。

IF 5

Pathobiology : journal of immunopathology, molecular and cellular biology Pub Date : 2012-01-01 Epub Date: 2011-12-28 DOI: 10.1159/000334089

Josefa Antonia Rodríguez, Liliana Galeano, Diana María Palacios, Constanza Gómez, Martha Lucía Serrano, María Mercedes Bravo, Alba Lucía Combita

{"title":"Altered HLA class I and HLA-G expression is associated with IL-10 expression in patients with cervical cancer.","authors":"Josefa Antonia Rodríguez, Liliana Galeano, Diana María Palacios, Constanza Gómez, Martha Lucía Serrano, María Mercedes Bravo, Alba Lucía Combita","doi":"10.1159/000334089","DOIUrl":"https://doi.org/10.1159/000334089","url":null,"abstract":"Although high-risk human papillomaviruses (HPVs) are an important risk factor in the etiopathogenesis of cervical cancer, increasing evidence suggests that the ability to avoid immune surveillance seems to be linked to the transforming potential of HPV and a rapid progression to cancer. In other cancer models, IL-10 contributes to impair anti-tumor immune response either by downregulating human leukocyte antigen Class I (HLA-I) expression or by increasing HLA-G expression. To comprehend how these alterations could contribute to evasion of immune surveillance in cervical cancer, we analyzed HLA-I, HLA-G and IL-10 expressions by immunohistochemistry in 63 biopsies from patients with cervical intraepithelial neoplasia III (CIN-III) and cervical cancer. Immunohistochemistry showed absent or weak HLA-I expression in 50/59 cases. In these cases, a high percentage had loss of heterozygosis. IL-10 and HLA-G expression were observed in 46.6 and 27.6% of cases, respectively. Concurrent upregulation of IL-10 was found in 87.5% of HLA-G positive cases (p = 0.000). Similarly, a significant association between IL-10 expression and HLA-I downregulation was found (p = 0.028). Finally, we observed higher HLA-G expression in patients with HLA-I downregulation than in those with normal HLA-I expression (p = 0.004). Our results suggest that, in cervical cancer, the IL-10 expression may induce an immunosuppressive environment by upregulating HLA-G expression and downregulating HLA class I expression.","PeriodicalId":244631,"journal":{"name":"Pathobiology : journal of immunopathology, molecular and cellular biology","volume":" ","pages":"72-83"},"PeriodicalIF":5.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000334089","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39966443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 60

Pathology of chronic constipation in pediatric and adult coloproctology. 儿童和成人结肠直肠慢性便秘的病理。

Pathobiology : journal of immunopathology, molecular and cellular biology Pub Date : 2005-03-09 DOI: 10.1159/isbn.978-3-318-01199-9

W. Meier-Ruge, E. Bruder

{"title":"Pathology of chronic constipation in pediatric and adult coloproctology.","authors":"W. Meier-Ruge, E. Bruder","doi":"10.1159/isbn.978-3-318-01199-9","DOIUrl":"https://doi.org/10.1159/isbn.978-3-318-01199-9","url":null,"abstract":"In colonic motility disorders, a pathohistological diagnosis based solely on formalin-fixed gut is often inconclusive. Classical histological techniques or immunohistochemistry represent a static staining. In contrast, native tissue submitted to enzyme histochemistry provides functional information about the effectiveness of the cellular performance. Routinely, a complementary set of reactions is performed and includes acetylcholinesterase (AChE), lactic and succinic dehydrogenase, as well as nitroxide synthase reactions. In this monograph, the whole spectrum of different anomalies of the colonic wall is illustrated in a systematic fashion: Hirschsprung's disease is characterized by an increase in AChE activity of parasympathetic nerve fibers of the rectosigmoid. In ultrashort Hirschsprung's disease, only enzyme histochemistry renders a reliable diagnosis possible in biopsies of the anal ring. Aganglionosis of the musculus corrugator cutis ani shows a localized increase of AChE activity in nerve fibers, similar to Hirschsprung's disease, not detectable in conventional histology. Immaturity, hypoganglionosis and neuronal dysganglionosis can be clearly recognized in dehydrogenase reactions. Enzyme histochemical reactions are complemented by picrosirius red staining for assessment of the collagen texture of the muscularis propria. Absence or intertenial interruption of the continuous connective tissue layer between circular and longitudinal muscle of the muscularis propria has been termed aplastic or atrophic desmosis, respectively. Many of the entities described are also observed in adults. Atrophic hypoganglionosis or atrophic desmosis with loss of the myenteric plexus connective tissue fascia is implied as a frequent cause of chronic constipation in adults. The essential contribution of a functional histopathological technique towards a reliable diagnosis of gut dysfunction in native tissue is extensively demonstrated in great detail in more than two hundred figures.","PeriodicalId":244631,"journal":{"name":"Pathobiology : journal of immunopathology, molecular and cellular biology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114581515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 70

Chemical characterization of peroxidized low-density lipoprotein in plasma and aortic atheroma. 血浆和主动脉粥样硬化中过氧化低密度脂蛋白的化学特征。

Pathobiology : journal of immunopathology, molecular and cellular biology Pub Date : 1994-09-01 DOI: 10.1016/0021-9150(94)93207-7

T. Kanazawa, T. Osanai, T. Uemura, K. Onodera, H. Metoki

引用次数: 0