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Application of 25-National Eye Institute Visual Function Questionnaire in ophthalmological patients 25份全国眼科学会视力功能问卷在眼科患者中的应用

ISP Medicine Pub Date : 2020-01-01 DOI: 10.52274/ISPMED20201025

Luciano Mesquite Simmo, Carissa Fouad Ibrahim, Senice Alvarenga Rodrigues Silva, Thai Nunes Andrade, Doora Faleiros Leite, Roberto Ferreira de Almeida Arajo

{"title":"Application of 25-National Eye Institute Visual Function Questionnaire in ophthalmological patients","authors":"Luciano Mesquite Simmo, Carissa Fouad Ibrahim, Senice Alvarenga Rodrigues Silva, Thai Nunes Andrade, Doora Faleiros Leite, Roberto Ferreira de Almeida Arajo","doi":"10.52274/ISPMED20201025","DOIUrl":"https://doi.org/10.52274/ISPMED20201025","url":null,"abstract":"Objective: To compare the vision-targeted health related quality of life (HRQOL) between neuro-ophthalmological patients and other eye diseases by the National Eye Institute 25-Item Visual Function Questionnaire. Methods: Cross sectional study with a control group and patients with the following pathologies: primary open-angle glaucoma (POAG), diabetic retinopathy (DR), age-related macular degeneration (ARMD), non-arteritic ischemic optic neuropathy (NAION), intracranial hypertension (IH), optic neuritis (ON), ptosis and cataract. Results: All comparisons of the subscales scores among the control group and the patient groups were statistically significant (p<0.05) except for “ocular pain” (p=0.160), “social functioning” (p=0.052) and “peripheral vision” (p=0.112). The control group had the best scores across all dimensions of the NEI VFQ-25. Interestingly, the ARMD and cataract groups presented the best and worst total scores of NEI VFQ-25, respectively. The lowest subscales scores were found in the cataract, in the NAION/ON, and in the POAG groups. Finally, the comparison between the NAION/ON/IH patients and the other eye diseases did not show statistical significance in any subscale. Conclusion: The NEI VFQ-25 showed the impact of various eye conditions in vision-targeted HRQOL, and no difference was measured between neuro-ophthalmological patients and other eye diseases","PeriodicalId":243745,"journal":{"name":"ISP Medicine","volume":"70 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114090528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rabbit Normal Regenerating Pancreas Extract induces rat BM-MSCs into Pancreatic Islet-like Structure Cells in vitro 兔正常再生胰腺提取物体外诱导大鼠骨髓间充质干细胞转化为胰岛样结构细胞

ISP Medicine Pub Date : 1900-01-01 DOI: 10.52274/ispmed20190908

Huifeng Wang, Yichen Xu, Liqing Wu, Yali Kou, Zhuo Wu, Wen-hong Zhao, Y. Wei, Hongjing Liu, Weiping Chen

{"title":"Rabbit Normal Regenerating Pancreas Extract induces rat BM-MSCs into Pancreatic Islet-like Structure Cells in vitro","authors":"Huifeng Wang, Yichen Xu, Liqing Wu, Yali Kou, Zhuo Wu, Wen-hong Zhao, Y. Wei, Hongjing Liu, Weiping Chen","doi":"10.52274/ispmed20190908","DOIUrl":"https://doi.org/10.52274/ispmed20190908","url":null,"abstract":"Bone marrow mesenchymal stem cells (BM-MSCs) could differentiate into Insulin Producing Cells(IPCs) with notable advantages. The present study tried to develop a method may be able to use a normal regenerating pancreas extract(N-RPE) medium to induce BM-MSCs into islet phenotype, in tests to assess how efficient method and simple duplicate the novel condition medium protocol is. Isolate and purify MSCs from rat bone marrow. BM-MSCs were differentiated into Adipogenic, Osteogenic and Myocardium and the lineages were assessed its multi-lineage potential. Islet differentiation medium, blending rabbit conditioned medium N-RPE, was administered to rat BM-MSCs. After 15 days, differentiation was evaluated by lineage-specific morphology and three stages could be observed: induced cells, islet like cells(ILCs) and islet like structures(ILSs). The morphology, SEM, DTZ staining, Mallory staining, HE staining and glucose stimulation demonstrated that the N-RPE could stimulate suitable development microenvironment to supply the islet differentiate from BM-MSCs. In addition, islet-related genes (ins/glu) expression and proteins(insulin/glucagon) expression suggested that culture medium rabbit N-RPE enhanced the rat BM-MSCs transdifferentiation efficiency. N-RPE derived from normal pancreas tissue could promote pancreas development of microenvironment and significantly enhance the transdifferentiation of BM-MSCs into ILSs. Results from this work will contribute to optimize the conditions of BM-MSCs and supply a new strategy for the development of islet tissue engineering.","PeriodicalId":243745,"journal":{"name":"ISP Medicine","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126700586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1