Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie最新文献
{"title":"Different appearance of parasitized erythrocytes in blood between normal and toxoplasma-infected rats after infection of Plasmodium berghei.","authors":"Y Omata, T Nakabayashi, N Suzuki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>When normal rats were infected with Plasmodium berghei (Pb), both IgG and IgM immunofluorescent antibody titers were found to rise in the 1st week with increase of parasitaemia. After reinoculation of P. berghei into Pb-immune rats, IgG titer was further increased remarkably, although no parasitaemia was observed. No elevation of IgM titer was found. In rats infected with Toxoplasma gondii (Tg), IgG and IgM antibody activities were demonstrated in the 1st to the 3rd week postinfection but only IgG titer was maintained to the 16th week. The challenge with T. gondii to Tg-immune rats stimulated the further increase in IgG titer but not in IgM. When Tg-immune rats were infected with P. berghei, little or no parasitaemia appeared. In infection of P. berghei in Tg-immune rats which were treated with anti-rat thymocyte serum (ATS) beforehand, highly increased parasitaemia was usually found in the rats as compared with that in ATS non-treated rats. Pb-immune macrophages were more effective in phagocytosis of Pb-parasitized erythrocytes in vitro than normal or Tg-immune macrophages. When Pb-parasitized erythrocytes were preincubated with fresh Pb-immune serum, the phagocytosis rate of macrophages was clearly heightened. It was observed that the phagocytic activity of normal macrophages to Pb-parasitized erythrocytes was stimulated by addition of the supernatant (lymphokines) taken from the incubation of Tg-immune lymphocytes with Tg-antigen or Pb-antigen.</p>","PeriodicalId":23838,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","volume":"244 2-3","pages":"362-73"},"PeriodicalIF":0.0,"publicationDate":"1979-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11596813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and characterization of influenza a viruses from wild free-flying ducks in Hokkaido, Japan.","authors":"H Kida, R Yanagawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three influenza A viruses possessing hemagglutinin-Hav7 and neuraminidase-N2 were isolated from 20 wild free-flying ducks in Hokkaido, Japan in autumn, 1977. The neuraminidase antigen of these viruses cross-reacted with the Asian influenza virus (H2 N2) but reacted scarcely with the Hong Kong strain (H3 N2Y in cross neuraminidase-inhibition tests.</p>","PeriodicalId":23838,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","volume":"244 2-3","pages":"135-43"},"PeriodicalIF":0.0,"publicationDate":"1979-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11708959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pneumocystis carinii pneumonia: epidemiology in Japan, and cyst concentration method.","authors":"Y Yoshida, T Ikai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pneumocystis carinii pneumonia has been markedly increasing recently in Japan. Most cases are seen after receiving anti-cancer and immunosuppressive therapy against their underlying diseases. No cases have been found in undernourished or premature infants. The authors proposed a new method which can concentrate the cysts efficiently from the human and animal lungs, and express the density of infection quantitatively at the same time. This method will especially be valuable to demonstrate the organism in light infection such as latent infection, after treatment, and so on.</p>","PeriodicalId":23838,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","volume":"244 2-3","pages":"405-10"},"PeriodicalIF":0.0,"publicationDate":"1979-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11528623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Blood cultures with the BACTEC 225 radiometric microbial growth detection system.","authors":"D Gröschel, R L Hopfer, J E French","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The BACTEC 225 automated radiometric blood culture system was compared with a conventional blood culture bottle method for its ability to improve the rapid laboratory diagnosis of bacteremia in cancer patients. The BACTEC 225, in combination with routine blind subcultures and smears of radiometrically negative culture vials, detected two thirds, of all positive cultures within 24 h and shortered the detection time generally by 24-48 h. With the recommended growth index setting of 30, radiometrically false-positive findings are rare and are usually due to leukocytosis resulting from infection or leukemia.</p>","PeriodicalId":23838,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","volume":"244 2-3","pages":"316-23"},"PeriodicalIF":0.0,"publicationDate":"1979-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11596811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tracing of pseudamonas aeruginosa infection by the use of commercial antisera and pyocin production and the evaluation of the results on the basis of the chi2 test.","authors":"V Sticht-Groh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One hundred randomly collected Psuedomonas aeruginosa isolates were examined during a two months period of time in order to determine their origin in a hospital environment. Initially, neither the source of the isolates, nor the patients' names were known. The serogroups were determined with commercial antisera, and the pyocin patterns were established with the simplified method of mitomycin C induced pyocin production. The results were analysed in the chi2 test. A highly significant probability value (p less than 00004) was taken as evidence of the identity of the isolates, especially among strains originating from the neurosurgical, surgical and medical intensive care units investigated. The simplified method of the originally devised mitomycin C induced pyocin production with a selected number of 'indicator' strains in conjunction with the initial serogroup determination by the commercially available antisera, allowed a rapid tracing of all Pseudomonas aeruginosa isolates. This combined method is recommended as an easy and useful epidemiological tool for the study of Pseudomonas aeruginosa hospital acquired infections.</p>","PeriodicalId":23838,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","volume":"244 2-3","pages":"240-50"},"PeriodicalIF":0.0,"publicationDate":"1979-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11708966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Serological cross-reactions of leptospiral lipopolysaccharide (F4) antigen.","authors":"B Adler, S Faine","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The serological specificity of leptospiral lipopolysaccharide (F4) antigen was examined by the technique of passive haemagglutination. F4 extracted from leptospiral serovars representative of several different serogroups showed wide cross reaction between serovars, including numerous one-way (non-reciprocal) reactions. The pattern of cross reaction was different to that of the standard leptospiral classification scheme.</p>","PeriodicalId":23838,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","volume":"244 2-3","pages":"291-301"},"PeriodicalIF":0.0,"publicationDate":"1979-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11313260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation of Francisella tularensis from mites Haemogamasus nidi and Laelaps hilaris in western slovakia.","authors":"J Lysý, J Nosek, V Vyrosteková, J Kovácik","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Isolating of F. tularensis from gamasid mites H. nidi parasitizing on the bank vole (Cl. glareolus) and L. hilaris on the common vole (M. arvalis) are reported. The epidemiological significance of this finding is discussed.</p>","PeriodicalId":23838,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","volume":"244 2-3","pages":"324-6"},"PeriodicalIF":0.0,"publicationDate":"1979-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11596812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I Tarnok, H Pechmann, U Krallmann-Wenzel, E Röhrscheidt, Z Tarnok
{"title":"[Nicotinamidase and the so-called pyrazinamidase in mycobacteria; the simultaneous occurrence of both activites (author's transl)].","authors":"I Tarnok, H Pechmann, U Krallmann-Wenzel, E Röhrscheidt, Z Tarnok","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nicotin- and the so-called pyrazinamidase (in the following: \"pyrazinamidase\") have been found in strains of four mycobacteria species, M. fortuitum, M. gastri, M. bovis and M. microti. These findings are in contradiction to those summarized in Bergey's Manual of Determinative Bacteriology (1974). The reason for the discrepancies is that the original method (Bönicke, 1961) for amidase determination has not taken the following aspects into consideration: a) The inducibility of the nicotin- and \"pyrazinamidase\" (example: M. fortuitum); b) The temperature sensitivity of these enzymes (M. gastri); c) The light sensitivity of nicotinamidase (in photochromogenic M. gastri strains); d) The optimal substrate concentration which must be at least 4 mM instead of 0,8 mm. The following consequences can be drawn for the taxonomy and biochemistry of the tested organisms: e) The species status of M. gastri should be annuled. The main difference between M. gastri and M. kansasii consists only of the non-agglutinability of M. gastri by anti-M. kansasii serum. \"Pyrazinamidase\" and also nitrate reductase (Tarnok et al., in press) are positive in strains of both species; f) M. bovis possesses nicotin- and \"pyrazinamidase\" as M. tuberculosis too. Thus, these two species are more closely related than suggested earlier; g) Till now, no Mycobacterium has been found showing nicotinamidase without \"pyrazinamidase\" activity (or vice versa). It seems to be very probable that nicotinamidase, an enzyme of low substrate specificity, is able to hydrolyze several compounds with a nicotinamide-like structure such as pyrazinamide. Thus, we suggest the annulment of the term pyrazinamidase or the employment of quotation marks (\"pyrazinamidase\") to show the fictitious value of this designation.</p>","PeriodicalId":23838,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","volume":"244 2-3","pages":"302-8"},"PeriodicalIF":0.0,"publicationDate":"1979-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11711404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J Dankert, W F Mensink, J G Aarnoudse, G J Meijer-Severs, H J Huisjes
{"title":"The prevalence of anaerobic bacteria in suprapubic bladder aspirates obtained from pregnant women.","authors":"J Dankert, W F Mensink, J G Aarnoudse, G J Meijer-Severs, H J Huisjes","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The incidence of asymptomatic bacteriuria due to aerobic, facultative anaerobic and strictly anaerobic bacteria during pregnancy was determined in 185 patients admitted for complications of pregnancy, using suprapubic bladder aspiration. Anaerobic bacterial counts of 103 per ml urine or higher were detected in 6.4 per cent of the specimens. The incidence of anaerobic bacteriuria seems comparable to that of bacteriuria due to aerobic and facultative anaerobic micro organisms. In our group of patients the incidence of the latter was 5.4%. The presence of anaerobic bacteria was not confined to the occurrence of a particular species. Peptostreptococcus species, Veillonella species, Bacteroides species, Eubacterium species, Clostridium species and Bifidobacterium species were isolated from specimens in concentrations of more than 103 per ml. Consequences of asymptomatic bacteriuria during pregnancy are totally uncertain. Further investigation is necessary to obtain insight into the effect of bacteriuria due to anaerobes upon the outcome of pregnancy.</p>","PeriodicalId":23838,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","volume":"244 2-3","pages":"260-7"},"PeriodicalIF":0.0,"publicationDate":"1979-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11596810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fundamental studies on macrophage migration inhibitory factor(s) in the supernatant from spleen cells in mice infected with Toxoplasma gondii.","authors":"I Igarashi, M Taguchi, N Suzuki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>When migration inhibitory factor (MIF) assay in vitro was conducted on the lymphokines (LKs), it was observed that the percentage of MIF activity was greatly increased from the 3rd to the 4th week postinfection. On the succeeding weeks there was a noticeable decrease in the MIF activity noted on the 8th week postinfection of Toxoplasma. MIF activity was examined at 1, 6, 12, 18, 24 and 48 hours in non-immune spleen cells as well as Toxoplasma immune spleen cells in the 2nd week after the final challenge inoculation. MIF activity in Toxoplasma immune spleen cells were 2, 21, 29, 54, 70 and 93 percentage, respectively. The MIF activity of hyperimmunized spleen cells produced an activity of approximately 50% at 18 hours as compared to the non-immune spleen cells. Characterization of the MIF was performed using Sephadex G-100 and DEAE Sephadex A 50. Two distinct peaks of MIF were identified and separated by Sephadex G-100 gel filtration, calculating molecular weights between 30,000 to 40,000 and 3,000 to 5,000, respectively. When the fast peak by Sephadex G-100 was eluted again in DEAE Sephadex A 50, the peak was separated into 4 units, all units showing MIF activity.</p>","PeriodicalId":23838,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie","volume":"244 2-3","pages":"374-82"},"PeriodicalIF":0.0,"publicationDate":"1979-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11596814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}