Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie最新文献_第8页

Interactions of the third component of complement (C3) with cross-linked dextran. IV. Adherence of lymphocytes to C3 coated dextran gel particles. 补体第三组分(C3)与交联右旋糖酐的相互作用。IV.淋巴细胞粘附在C3包被的葡聚糖凝胶颗粒上。

Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie Pub Date : 1975-07-01

W Knapp

{"title":"Interactions of the third component of complement (C3) with cross-linked dextran. IV. Adherence of lymphocytes to C3 coated dextran gel particles.","authors":"W Knapp","doi":"","DOIUrl":"","url":null,"abstract":"In previous studies we could show that the third component of complement (C3) is bound from normal serum to cross-linked dextran (Sephadex) after activation via an alternate pathway. Data presented in this paper demonstrate that a subpopulation of human lymphocytes adheries to C3 coated Sephadex particles. The adhering subpopulation of lymphocytes is identical with or overlaps extensively with lymphocytes bearing easily detectable membrane Ig. This phenomenon can be used for the fractionation of lymphocyte subpopulations. After a single passage of tonsillar lymphocytes through C3 coated Sephadex bead columns, no membrane Ig positive lymphocytes were detectable in the effluent. 97-100% of effluent cells formed rosettes with untreated sheep red blood cells, thus resembling pure T cells. Apart from that, the use of C3 coated dextran gel particles allows also the recovery of adherent cells after dextranase digestion of the dextran gel particles. An investigation of retained cells showed enrichment of B cells, but also T cells were demonstrable. The retention of these T cells is probably due to nonspecific interactions.","PeriodicalId":23768,"journal":{"name":"Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie","volume":"149 5","pages":"428-39"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11345043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rheumatoid factor appearance in Micrococcus lysodeikticus immunization and its interference with allotype specific reactions. 溶血微球菌免疫过程中类风湿因子的出现及其对同种异型特异性反应的干扰。

Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie Pub Date : 1975-07-01

R Hamers, C Hamers-Casterman, W van der Loo, A D Strosberg, P De Baetselier

引用次数: 0

Interaction of peptidoglycans with anti-IgGs and with complement. 肽聚糖与抗igg和补体的相互作用。

Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie Pub Date : 1975-07-01

V A Bokisch

{"title":"Interaction of peptidoglycans with anti-IgGs and with complement.","authors":"V A Bokisch","doi":"","DOIUrl":"","url":null,"abstract":"This report describes the interaction of peptidoglycan (Streptococcus group A, Staphylococcus epidermidis and Micrococcus lysodeikticus) with 2 serum mediator systems, namely with the anti-IgG system and with complement. The observation that the majority of rabbits hyperimmunized with A-variant streptococcal vaccine produced anti-group carbohydrate antisera containing anti-IgGs and antibodies directed to peptidoglycan suggested that the production of these 2 latter antibodies was related. This view was supported by the finding of a monoclonal 7S anti-IgG with antibody specificity for the pentapeptide of peptidoglycan as evidenced by inhibition of the coprecipitation of 7S anti-IgG with antigen-antibody complexes by the pentapeptide. Inhibition of the anti-idiotype reaction by the pentapeptide provided further evidence for the antibody specificity of 7S anti-IgG for peptidoglycan. When added to normal human sera all peptidoglycan preparations inhibited the hemolytic activity of the sera. Consumption of C3 in C2 deficient serum and consumption of C2 in normal serum indicated the activation of both known complement pathways. Activation of the classical pathway of complement was more efficient since 50 mug of peptidoglycan consumed approximately 70% of C2 per ml normal serum whereas more than 2 mg of the same preparations was required to inactivate 17-24% of C3 in C2 deficient sera. Each of the different peptidoglycan preparations consumed similar amounts of complement in all 20 sera tested. This finding suggested that activation of the classical complement pathway by peptidoglycan was not mediated by anti-peptidoglycan antibodies present in only 20-40% of normal human sera.","PeriodicalId":23768,"journal":{"name":"Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie","volume":"149 2-4","pages":"320-30"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11345985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibodies to peptidoglycan in the sera from population surveys. 人群调查血清中肽聚糖抗体。

Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie Pub Date : 1975-07-01

W Schachenmayr, B Heymer, O Haferkamp

引用次数: 0

Estimates of the porosity of Bacillus licheniformis and Bacillus subtilis cell walls. 地衣芽孢杆菌和枯草芽孢杆菌细胞壁孔隙率的估计。

Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie Pub Date : 1975-07-01

R C Hughes, P F Thurman, E Stokes

引用次数: 0

Low molecular weight water-soluble peptidoglycans as adjuvants and immunostimulants. 低分子量水溶性肽聚糖作为佐剂和免疫刺激剂。

Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie Pub Date : 1975-07-01

P Jollès, D Migliore-Samour, R Maral, F Floc'h, G H Werner

引用次数: 0

Immunological activity of the peptidoglycan. 肽聚糖的免疫活性。

Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie Pub Date : 1975-07-01

R M Krause

{"title":"Immunological activity of the peptidoglycan.","authors":"R M Krause","doi":"","DOIUrl":"","url":null,"abstract":"Studies on the immunology of peptidoglycan received impetus from the initial observation that the rabbit Group A-variant streptococcal antisera were a rich source of antibodies to peptidoglycan. Indeed, quantitative precipitin studies revealed concentrations as high as 7-10 mg/ml of antiserum. The amount of antibody after Group A-variant streptococcal immunization is much greater than the amount in the sera following immunization of rabbits with the Group A or C streptococci. Furthermore, earlier studies had shown that the purified peptidoglycan obtained as a residue following extraction of streptococcal cell walls with hot formamide was a poor antigen. Both the hexosamine polymer and the peptide moiety are antigenic. Use of the solid phase synthesized pentapeptide L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala and related similar peptides facilitated the determination of the fine structure of the immunodominant site of pentapeptide. The evidence points to the C-terminal D-Ala-D-Ala as the immunodominant determinant. Because of the similarity of the peptidoglycans of a number of different bacteria, it would be anticipated that they would cross-react immunologically, and this has been shown to be the case. The biological and medical significance of antibodies to peptidoglycan has yet to be determined. Certainly the exposure to this ubiquitous substance which occurs in all the indigenous bacteria of the respiratory and the gastrointestinal tract must mean that from an early age and through advancing years there is a constant stimulation of the immune response to peptidoglycan. Because the immunochemistry of these substances is now firmly established, there is a scientific basis for proceeding with the medical and biological implications of peptidoglycan immunity.","PeriodicalId":23768,"journal":{"name":"Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie","volume":"149 2-4","pages":"136-50"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11274171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interactions of the third component of complement (C3) with cross-linked dextran. III. Isolation and characterization of interacting components after enzymatic digestion of complement coated cross-linked dextran. 补体第三组分(C3)与交联右旋糖酐的相互作用。3补体包被交联葡聚糖酶切后相互作用组分的分离与表征。

Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie Pub Date : 1975-07-01

W Knapp

引用次数: 0

Chemical structure of the cell wall of Mycobacterium tuberculosis var. bovis, strain BCG. 牛结核分枝杆菌卡介苗株细胞壁的化学结构。

Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie Pub Date : 1975-07-01

J F Petit, J Wietzerbin, B C Das, E Lederer

引用次数: 0

Peripheral blood T and B lymphocytes in men in different age groups. 不同年龄组男性外周血T淋巴细胞和B淋巴细胞的变化。

Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie Pub Date : 1975-07-01

G Cohnen, W Augener, A Reuter, G Brittinger

引用次数: 0