Journal of International Society of Microbiota最新文献

Candidate probiotic strains, with immunomodulatory and antioxidant effects, isolated from traditional Italian dairy products 从意大利传统乳制品中分离出具有免疫调节和抗氧化作用的候选益生菌菌株

Journal of International Society of Microbiota Pub Date : 2016-09-19 DOI: 10.18143/JISM_V3I1

G. Curone, P. Cremonesi, F. Riva, M. F. Addis, L. Turin, C. Pollera, M. Severgnini, J. Filipe, D. Calonzi, D. Vigo, P. Moroni, V. Bronzo, B. Castiglioni

{"title":"Candidate probiotic strains, with immunomodulatory and antioxidant effects, isolated from traditional Italian dairy products","authors":"G. Curone, P. Cremonesi, F. Riva, M. F. Addis, L. Turin, C. Pollera, M. Severgnini, J. Filipe, D. Calonzi, D. Vigo, P. Moroni, V. Bronzo, B. Castiglioni","doi":"10.18143/JISM_V3I1","DOIUrl":"https://doi.org/10.18143/JISM_V3I1","url":null,"abstract":"Cancer is a multifactorial disease and inflammation could be the 10th hallmark of cancer. Increased systemic inflammation is almost, if not always, a sign of dysbiosis and increased translocation of of bacterial origin, such as endotoxin. Therefore, therapeutic manipulations of intestinal bacteria could reverse the inflammatory responses and restore mucosal homeostasis. Thus, we evaluated the role of a fresh Pasta Filata cheese of the Alto Molise area called “Stracciata”, made from milk of cows fed by grazing and with local dry forages, as a source of potentially probiotic microorganisms. Molecular characterization by sequencing of the 16S rRNA gene were carried out on all bacteria isolated from fresh Pasta Filata cheese manufactured with natural starter cultures. Transmembrane electrical potential (ΔΨ) assay, antioxidant system activity, Glutathione disulphide (GSSG)/reduced Glutathione (GSH) ratio and nucleotides pool were measured by fluorescence techniques and HPLC. Moreover, the ability of bacterial isolates to stimulate IL-10 and TNF-α production by human peripheral blood mononuclear cells (PBMC) was determined in vitro by ELISA kits. We demonstrate how bacteria isolate by Stracciata cheese or their metabolites regulate immunomodulatory effects. Further studies are opportune for conferring therapeutic strategies to traditional dairy products in bacteria-metabolite-immune system axis contest.","PeriodicalId":237110,"journal":{"name":"Journal of International Society of Microbiota","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115577674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

MyNewGut: Insulin resistance as key factor for linking modulation of gut microbiome to health claims and dietary recommendations to tackle obesity MyNewGut:胰岛素抵抗是将肠道微生物群调节与健康声明和饮食建议联系起来的关键因素，以解决肥胖问题

Journal of International Society of Microbiota Pub Date : 2016-09-06 DOI: 10.4172/2324-9323-C1-004

S. Loman, J. Kamp

{"title":"MyNewGut: Insulin resistance as key factor for linking modulation of gut microbiome to health claims and dietary recommendations to tackle obesity","authors":"S. Loman, J. Kamp","doi":"10.4172/2324-9323-C1-004","DOIUrl":"https://doi.org/10.4172/2324-9323-C1-004","url":null,"abstract":"Abstract Background: Current dietary recommendations addressing obesity do not as yet include recommendations pertaining to the gut microbiome, nor have microbiome-related health claims made on foods been proposed as yet. Scope: The EU FP7-funded MyNewGut project aims to develop guidance for the establishment of dietary recommendations related to the role microbiome in the onset and development of obesity. Key findings: Of all the physiological impacts resulting from changes in the microbiome, insulin resistance is the most direct diet-modifiable parameter related to obesity. Therefore, improving insulin resistance is considered to be the key health benefit conferred by the targeted modulation of the gut microbiome. Conclusions: In order to facilitate guidance for the development of public health and dietary recommendations, as well as for health claim substantiation related to the gut microbiome, foods containing microbiome-targeting dietary fibers and microorganisms will be developed and studies with these foods should provide for the total body of clinical evidence specifically addressing the central theme of ‘insulin resistance’ in obesity.","PeriodicalId":237110,"journal":{"name":"Journal of International Society of Microbiota","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126160980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bowel cleansing as influencing factor of gut microbiota: the state of the art 肠道清洁作为肠道菌群的影响因素:最新进展

Journal of International Society of Microbiota Pub Date : 2016-05-03 DOI: 10.18143/JISM_V2I1.967

Marco Toscano, L. Drago

引用次数: 0

Association of dietary type with fecal microbiota and short chain fatty acids in vegans and omnivores 素食者和杂食动物的饮食类型与粪便微生物群和短链脂肪酸的关系

Journal of International Society of Microbiota Pub Date : 2016-02-19 DOI: 10.18143/JISM_V1I1.782

Alina Reiss, Manuela Jacobi, K. Rusch, Schwiertz Andreas

{"title":"Association of dietary type with fecal microbiota and short chain fatty acids in vegans and omnivores","authors":"Alina Reiss, Manuela Jacobi, K. Rusch, Schwiertz Andreas","doi":"10.18143/JISM_V1I1.782","DOIUrl":"https://doi.org/10.18143/JISM_V1I1.782","url":null,"abstract":"Diet plays an essential role in modulating the bacterial composition within the gut. Data on the effect of vegan diets on the faecal microbiota and the production of short chain fatty acids are few. The present study compared the faecal microbiota of vegans and omnivores in Germany. Faecal samples were obtained from ten vegan and ten omnivore subjects with a similar social background. Macronutrient intake and anthropometric data were collected. The faecal microbiota of interest was quantified by real-time PCR with SYBR green using primers for the main bacterial phyla and genera. In addition, the production of the short chain fatty acids acetate, propionate, butyrate, valerate and the branched chain fatty acids iso-valerate and iso-butyrate were measured by gas chromatography. The faecal microbiota of the vegan group was enriched for the phyla Verrucomicrobia while it was diminished for the phylum Proteobacteria and lactic acid bacteria. The relative faecal concentrations of short-chain fatty acids did not differ between the groups. However, in the vegan group a lower amount of brain-chain fatty-acids, acetate and propionate, could be observed. Maintaining a vegan diet results in a shift in the microbiota and the production of short chain fatty acids. It remains to be determined how these shifts affect the host metabolism and disease risk.","PeriodicalId":237110,"journal":{"name":"Journal of International Society of Microbiota","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132683284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16