Candidate probiotic strains, with immunomodulatory and antioxidant effects, isolated from traditional Italian dairy products

Abstract

Cancer is a multifactorial disease and inflammation could be the 10th hallmark of cancer. Increased systemic inflammation is almost, if not always, a sign of dysbiosis and increased translocation of of bacterial origin, such as endotoxin. Therefore, therapeutic manipulations of intestinal bacteria could reverse the inflammatory responses and restore mucosal homeostasis. Thus, we evaluated the role of a fresh Pasta Filata cheese of the Alto Molise area called “Stracciata”, made from milk of cows fed by grazing and with local dry forages, as a source of potentially probiotic microorganisms. Molecular characterization by sequencing of the 16S rRNA gene were carried out on all bacteria isolated from fresh Pasta Filata cheese manufactured with natural starter cultures. Transmembrane electrical potential (ΔΨ) assay, antioxidant system activity, Glutathione disulphide (GSSG)/reduced Glutathione (GSH) ratio and nucleotides pool were measured by fluorescence techniques and HPLC. Moreover, the ability of bacterial isolates to stimulate IL-10 and TNF-α production by human peripheral blood mononuclear cells (PBMC) was determined in vitro by ELISA kits. We demonstrate how bacteria isolate by Stracciata cheese or their metabolites regulate immunomodulatory effects. Further studies are opportune for conferring therapeutic strategies to traditional dairy products in bacteria-metabolite-immune system axis contest.