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Chinese Journal of Agricultural Biotechnology最新文献

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Construction of conjugal transfer system of Streptomyces cinnamonensis and effect of PCR-mediated nsdA gene disruption on its secondary metabolism 肉桂链霉菌偶联转移体系的构建及pcr介导的nsdA基因破坏对其次生代谢的影响
Chinese Journal of Agricultural Biotechnology Pub Date : 2008-04-01 DOI: 10.1017/S1479236208002106
Chen Fen, Xiong Wei, M. Yong, Fan Yu-Qing, Liang Yunxiang, L. He-Ping, Xing Ren-Chang, Zheng Ying-hua
{"title":"Construction of conjugal transfer system of Streptomyces cinnamonensis and effect of PCR-mediated nsdA gene disruption on its secondary metabolism","authors":"Chen Fen, Xiong Wei, M. Yong, Fan Yu-Qing, Liang Yunxiang, L. He-Ping, Xing Ren-Chang, Zheng Ying-hua","doi":"10.1017/S1479236208002106","DOIUrl":"https://doi.org/10.1017/S1479236208002106","url":null,"abstract":"Intergeneric transfer of plasmid vectors pSET152 and pHL212 from donor Escherichia coli ET12567(pUZ8002) and S17-1 to Streptomyces cinnamonensis were demonstrated and optimized. The nsdA gene disruption structure was constructed by PCR-targeting system and then introduced into S. cinnamonensis BIB2005 through intergeneric conjugal transfer. After PCR analysis, the screened AprRKanS conjugant BIB309 was confirmed to be the nsdA mutant. Compared with the start strain, the yield of monensin of the nsdA mutant BIB309 increased 270 percent in the level of flask.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116867412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening, cloning and sequence analysis of gBTN1A1 gene in mammary gland of Xinong Saanen goat 西农萨嫩山羊乳腺gBTN1A1基因的筛选、克隆及序列分析
Chinese Journal of Agricultural Biotechnology Pub Date : 2008-04-01 DOI: 10.1017/S1479236208001940
Zhang Lijuan, Wu Hui-juan, Luo Jun, L. Junxia, Z. Ning, Wang Hai-bing, Shan Cui-yan, Y. Gang
{"title":"Screening, cloning and sequence analysis of gBTN1A1 gene in mammary gland of Xinong Saanen goat","authors":"Zhang Lijuan, Wu Hui-juan, Luo Jun, L. Junxia, Z. Ning, Wang Hai-bing, Shan Cui-yan, Y. Gang","doi":"10.1017/S1479236208001940","DOIUrl":"https://doi.org/10.1017/S1479236208001940","url":null,"abstract":"","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126891308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Display of H5N1 Avian influenza virus haemagglutinin HA1 on Bacillus thuringiensis cell surface and its immunogenicity for mice H5N1禽流感病毒血凝素HA1在苏云金芽孢杆菌细胞表面的显示及其小鼠免疫原性
Chinese Journal of Agricultural Biotechnology Pub Date : 2007-12-01 DOI: 10.1017/S1479236207001702
L. Mei, Lu Lin-Jing, Huang Jun-yan, Zhang Shu-huan, Bi Ding-ren, S. Ming
{"title":"Display of H5N1 Avian influenza virus haemagglutinin HA1 on Bacillus thuringiensis cell surface and its immunogenicity for mice","authors":"L. Mei, Lu Lin-Jing, Huang Jun-yan, Zhang Shu-huan, Bi Ding-ren, S. Ming","doi":"10.1017/S1479236207001702","DOIUrl":"https://doi.org/10.1017/S1479236207001702","url":null,"abstract":"S-layer protein CTC surface display system of Bacillus thuringiensis (Bt) was used to test the possibility of displaying H5N1 Avian influenza virus haemagglutinin HA1 on B. thuringiensis cell surface. Two recombinant plasmids were constructed by replacing the central part below the surface anchor sequence slh of S-layer protein gene ctc with part ha1 gene (halp ). The two resulting plasmids were pCTC-HA1P (harboring fusion gene ctc-halp ) and pCSHA1P (harboring fusion gene csa-ctc-halp, csa represents csaAB operon which was very important to the anchoring of S-layer protein on the bacterial cell surface). Two recombinant B. thuringiensis strains were constructed by electro-transferring recombinant plasmids to B. thuringiensis plasmid-free derivative strain BMB171. The resulting strains were CH (harboring pCSHA1P) and BCCH (harboring pCTC-HA1P as well as the plasmid pMIL-CSA which carried csaAB operon). The vegetative cells of CH and BCCH were used as antigens of haemagglutination (HA) assay and haemagglutination inhibition (HI) assay. HA assay showed recombinant HA1 proteins were successfully displayed on the cell surface of CH and BCCH, respectively. HI assay showed recombinant HA1 proteins displayed on the cell surface were specific to standard positive HI (haemagglutination inhibition test) serum of subtype H5 AIV. After immunizing mice with vegetative cells of CH and BCCH respectively, CH and BCCH all elicited a humoral response to HA1 and exhibited immunogenicity as assayed by enzyme-linked immunosorbent assay (ELISA). ELISA also showed CH exhibited a higher immunogenicity than BCCH. The strategy developed in this study gives a possibility to generate heat-stable, oral, veterinary vaccine against AIV with B. thuringiensis S-layer protein CTC surface display system.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128845698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression of H5N1 Avian influenza virus haemagglutinin in a baculovirus expression system H5N1禽流感病毒血凝素在杆状病毒表达系统中的表达
Chinese Journal of Agricultural Biotechnology Pub Date : 2007-12-01 DOI: 10.1017/S1479236207001817
Dun Jifeng, Pu Juan, Z. Yingchun, L. Jinhua
{"title":"Expression of H5N1 Avian influenza virus haemagglutinin in a baculovirus expression system","authors":"Dun Jifeng, Pu Juan, Z. Yingchun, L. Jinhua","doi":"10.1017/S1479236207001817","DOIUrl":"https://doi.org/10.1017/S1479236207001817","url":null,"abstract":"The haemagglutinin (HA) gene of H5N1 Avian influenza virus (AIV) was amplified from the plasmid pGEM-HA and cloned into the baculovirus transfer plasmid pFastBacHT to construct the recombinant transfer vector pFastBacHT-HA. The pFastBacHT-HA was transformed into DH10Bac competent cells, transposed with a shuttle vector (Bacmid) and the transposition rBacmid-HA was constructed. The recombinant baculovirus was harvested from sf9 cells transfected with rBacmid-HA. The expressed HA protein was identified and analysed by SDS–PAGE, Western blot and haemadsorption assay. The 66 kDa protein could only react with chicken serum positive to H5 subtype AIV. The haemadsorption assay showed that the sf9 cells infected with rBacmid-HA baculovirus could absorb chicken red blood cells. These results indicated that the HA protein was successfully expressed in sf9 cells, with reaction specificity to H5 subtype antiserum.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117303592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Cloning of ACO gene and inhibition of ethylene evolution in tomatoes with RNAi 用RNAi技术克隆ACO基因及抑制番茄乙烯进化
Chinese Journal of Agricultural Biotechnology Pub Date : 2007-12-01 DOI: 10.1017/S1479236207001775
Cheng Yin-hua, Ouyang Bo, Li Hanxia, Ye Zhibiao
{"title":"Cloning of ACO gene and inhibition of ethylene evolution in tomatoes with RNAi","authors":"Cheng Yin-hua, Ouyang Bo, Li Hanxia, Ye Zhibiao","doi":"10.1017/S1479236207001775","DOIUrl":"https://doi.org/10.1017/S1479236207001775","url":null,"abstract":"","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114841922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
QTL identification for seed setting rate of rice in various environments 不同环境下水稻结实率的QTL鉴定
Chinese Journal of Agricultural Biotechnology Pub Date : 2007-12-01 DOI: 10.1017/S1479236207001970
C. Qingquan, Yu Si-Bin, Li Chunhai, Mou Tong-min
{"title":"QTL identification for seed setting rate of rice in various environments","authors":"C. Qingquan, Yu Si-Bin, Li Chunhai, Mou Tong-min","doi":"10.1017/S1479236207001970","DOIUrl":"https://doi.org/10.1017/S1479236207001970","url":null,"abstract":"","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128867473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Construction and evaluation of near-isogenic lines for major QTLs of basal root thickness and 1000-grain-weight in lowland and upland rice 陆稻基根粗和千粒重主要qtl近等基因系的构建与评价
Chinese Journal of Agricultural Biotechnology Pub Date : 2007-12-01 DOI: 10.1017/S1479236207001787
L. Lifeng, Zhang Hongliang, Mu Ping, Qu Yan-ying, Li Zichao
{"title":"Construction and evaluation of near-isogenic lines for major QTLs of basal root thickness and 1000-grain-weight in lowland and upland rice","authors":"L. Lifeng, Zhang Hongliang, Mu Ping, Qu Yan-ying, Li Zichao","doi":"10.1017/S1479236207001787","DOIUrl":"https://doi.org/10.1017/S1479236207001787","url":null,"abstract":"The development of near-isogenic lines (NILs) is one of the important foundations for the establishment of molecular linkage map, the mapping of quantitative trait locus(QTL) and molecular marker-assisted selection (MAS). In the present study, NILs for two major QTLs of basal root thickness(BRT) and 1000-grain-weight(TGW) were obtained through the foreground selection for the target QTL, the background selection for the genome of the recurrent parents of 3 backcross populations( BC1F1, BC2F1 and BC3F1) and phenotypic selection for the population of BC3F2. There were 9 BRT QTL-NILs with BRT ranges of 1.07~1.16 mm, over the recurrent parent by 6.11%~15.18% and an average recovering ratio of genetic background (RRGB)of 97.22%; eleven NILs of TGW QTL with ranges of 21.25～26.25 g, over the recurrent parent by 7.05%～32.16% and 95.97% of RRGB.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129816564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
PCR–ELISA method for Mycoplasma contamination detection in veterinary vaccines 兽医疫苗支原体污染检测的PCR-ELISA方法
Chinese Journal of Agricultural Biotechnology Pub Date : 2007-12-01 DOI: 10.1017/S1479236207001593
Z. Ying, Jiang Jun-bing, Liu Jin-ping, Jia Li-yan
{"title":"PCR–ELISA method for Mycoplasma contamination detection in veterinary vaccines","authors":"Z. Ying, Jiang Jun-bing, Liu Jin-ping, Jia Li-yan","doi":"10.1017/S1479236207001593","DOIUrl":"https://doi.org/10.1017/S1479236207001593","url":null,"abstract":"A polymerase chain reaction–enzyme-linked immunosorbent assay (PCR-ELISA) method was set up to detect Mycoplasma in live vaccines and to establish the optimal experimental conditions. According to the 16S rRNA gene sequences published in GenBank, which include Mycoplasma gallisepticum of chicken and M. hyopneumoniae , M. hyosynoviae and M. flocculare of swine (submitted nos: Mg AY744942, Mhp AE017244, Mhs AY973563 and Mf X63377), PCR primers, labelled with digoxin (Dig), and probe, labelled with biotin, were designed using the software DNAstar and Primer Premier 5.0. The system and conditions of PCR–ELISA were optimized using purified genomic DNA extracted from Mycoplasma as a template. Samples of 30 batches of Newcastle disease live vaccines (I strain), 30 batches of Newcastle disease live vaccines (La Sota strain) and 30 batches of swine fever live vaccine were tested by PCR-ELISA. Results showed that the detection rate of Mycoplasma contamination from different vaccines was higher (36.5%) with this technique than with PCR (24.4%). The PCR–ELISA appeared to be a simple, fast and reliable method for qualitative and quantitative analysis of Mycoplasma . The practical use of PCR–ELISA as a kit to detect Mycoplasma contamination in live vaccine seems very promising.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122396487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic diversity analysis of diazotrophs in the rice rhizosphere 水稻根际重氮营养体遗传多样性分析
Chinese Journal of Agricultural Biotechnology Pub Date : 2007-12-01 DOI: 10.1017/S1479236207001982
C. Bin, Zheng Si-ping, Zhou Li-juan, L. Zhimin, Song Yana, Zheng Wei-wen
{"title":"Genetic diversity analysis of diazotrophs in the rice rhizosphere","authors":"C. Bin, Zheng Si-ping, Zhou Li-juan, L. Zhimin, Song Yana, Zheng Wei-wen","doi":"10.1017/S1479236207001982","DOIUrl":"https://doi.org/10.1017/S1479236207001982","url":null,"abstract":"The genetic diversity of dinitrogen-fixing bacteria associated with rice ( Oryza sativa ) was assessed by a polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) approach on the nifH gene amplified directly from DNA extracted from washed rice roots and rhizospheric soil. Restriction digestion with the enzymes Mnl I and Hae III was performed to characterize 54 cloned nifH PCR products. RFLP profiles were clustered and analysed with the UPGMA program. Eight pairs of similar RFLP patterns (similarity>50%) and two pairs of homologous RFLP patterns (100% identity) were found from the washed roots and the rhizospheric soil, respectively. Three specific diazotrophic patterns were found from rhizospheric soil and rice roots. The analyses have revealed the presence of different nifH types, which appear to be significant components of the diazotrophic community in paddy fields, indicating that some of the diazotrophs may colonize the inside and the surface of the rice roots.","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121154277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Cloning and expression of Ma duck interleukin-18 mature protein gene and biological activity detection 麻鸭白介素-18成熟蛋白基因的克隆、表达及生物活性检测
Chinese Journal of Agricultural Biotechnology Pub Date : 2007-12-01 DOI: 10.1017/S1479236207001842
Chen Hongying, Cui Bao-an, Xia PingAn, Liu Xinsheng, Yang Mingfan, Zheng Lanlan
{"title":"Cloning and expression of Ma duck interleukin-18 mature protein gene and biological activity detection","authors":"Chen Hongying, Cui Bao-an, Xia PingAn, Liu Xinsheng, Yang Mingfan, Zheng Lanlan","doi":"10.1017/S1479236207001842","DOIUrl":"https://doi.org/10.1017/S1479236207001842","url":null,"abstract":"","PeriodicalId":236932,"journal":{"name":"Chinese Journal of Agricultural Biotechnology","volume":"120 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116368233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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