Weed SciencePub Date : 2022-12-23DOI: 10.1017/wsc.2022.67
Katie Martin, B. Hanson
{"title":"Determining How Almond (Prunus dulcis) Harvest and Processing Contributes to Low Levels of Glyphosate and Glufosinate Residues in Almonds","authors":"Katie Martin, B. Hanson","doi":"10.1017/wsc.2022.67","DOIUrl":"https://doi.org/10.1017/wsc.2022.67","url":null,"abstract":"Abstract California is the top producer of almonds [Prunus dulcis (Mill.) D.A. Webb] worldwide, generating more than $6 billion in revenue in 2020; the European Union (EU) is the primary importer of California almonds. Weed control in almond orchards is an important part of the preharvest process, because weeds can interfere with harvest equipment and host diseases. Glyphosate and glufosinate are broad-spectrum herbicides commonly used for preharvest weed control. Global differences in maximum residue limits (MRLs) and regulated compounds can pose a challenge for growers who rely on broad-spectrum herbicides such as glyphosate and glufosinate for preharvest weed control. The EU MRL for glyphosate and total glufosinate is currently 0.1 mg kg–1. The U.S. MRL for total glyphosate is 1 mg kg–1, and total glufosinate is 0.5 mg kg–1. An 8-wk field experiment, from spray to harvest, was conducted in an 8-ha commercial orchard to evaluate the potential contribution of the preharvest herbicide treatment to low levels of herbicide residue in almonds. Then, the same batch of almonds was followed through a commercial processing facility to evaluate the potential movement of herbicide residues from soil, debris, and hulls to almond kernels during processing. Glyphosate was not detected in any almond kernel samples at the end of processing. A glufosinate metabolite, 3-(methylphosphinico)propionic acid (MPP), was detected in kernels at the end of processing at about 0.1 mg kg–1, which is above the EU MRL for total glufosinate. Almonds sampled directly from the tree, without any contact with soil, were found to have elevated MPP residues. This indicates glufosinate or MPP translocation may be a factor in low-level glufosinate residues detected in almonds in some EU exports.","PeriodicalId":23688,"journal":{"name":"Weed Science","volume":"71 1","pages":"69 - 76"},"PeriodicalIF":2.5,"publicationDate":"2022-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46577604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weed SciencePub Date : 2022-12-19DOI: 10.1017/wsc.2022.68
E. Jones, R. Austin, J. Dunne, C. Cahoon, Katherine M. Jennings, R. León, W. Everman
{"title":"Utilization of Image-Based Spectral Reflectance to Detect Herbicide Resistance in Glufosinate-Resistant and Glufosinate-Susceptible Plants: A Proof of Concept","authors":"E. Jones, R. Austin, J. Dunne, C. Cahoon, Katherine M. Jennings, R. León, W. Everman","doi":"10.1017/wsc.2022.68","DOIUrl":"https://doi.org/10.1017/wsc.2022.68","url":null,"abstract":"Abstract Glufosinate is an effective postemergence herbicide, and overreliance on this herbicide for weed control is likely to increase and select for glufosinate-resistant weeds. Common assays to confirm herbicide resistance are dose–response and molecular sequencing techniques; both can require significant time, labor, unique technical equipment, and a specialized skillset to perform. As an alternative, we propose an image-based approach that uses a relatively inexpensive multispectral sensor designed for unmanned aerial vehicles to measure and quantify surface reflectance from glufosinate-treated leaf disks. Leaf disks were excised from a glufosinate-resistant and glufosinate-susceptible corn (Zea mays L.), cotton (Gossypium hirsutum L.), and soybean [Glycine max (L.) Merr.] varieties and placed into a 24-well plate containing eight different concentrations (0 to 10 mM) of glufosinate for 48 h. Multispectral images were collected after the 48-h incubation period across five discrete wave bands: blue (475 to 507 nm), green (560 to 587 nm), red (668to 682 nm), red edge (717 to 729 nm), and near infrared (842 to 899 nm). The green leaf index (GLI; a metric to measure chlorophyll content) was utilized to determine relationships between measured reflectance from the tested wave bands from the treated leaf disks and the glufosinate concentration. Clear differences of spectral reflectance were observed between the corn, cotton, and soybean leaf disks of the glufosinate-resistant and glufosinate-susceptible varieties at the 10 mM concentration for select wave bands and GLI. Leaf disks from two additional glufosinate-resistant and glufosinate-susceptible varieties of each crop were subjected to a similar assay with two concentrations: 0 and 10 mM. No differences of spectral reflectance were observed from the corn and soybean varieties in all wave bands and the GLI. The leaf disks of the glufosinate-resistant and glufosinate-susceptible cotton varieties were spectrally distinct in the green, blue, and red-edge wave bands. The results provide a basis for rapidly detecting glufosinate-resistant plants via spectral reflectance. Future research will need to determine the glufosinate concentrations, useful wave bands, and susceptible/resistant thresholds for weeds that evolve resistance.","PeriodicalId":23688,"journal":{"name":"Weed Science","volume":"71 1","pages":"11 - 21"},"PeriodicalIF":2.5,"publicationDate":"2022-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43712933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weed SciencePub Date : 2022-12-15DOI: 10.1017/wsc.2022.66
Haixia Zhu, Hongyu Chen, Yongqiang Ma, Q. Guo
{"title":"Identification and Extraction of Herbicidal Compounds from Metabolites of Trichoderma Polysporum HZ-31","authors":"Haixia Zhu, Hongyu Chen, Yongqiang Ma, Q. Guo","doi":"10.1017/wsc.2022.66","DOIUrl":"https://doi.org/10.1017/wsc.2022.66","url":null,"abstract":"Abstract Trichoderma polysporum (Link) Rifai HZ-31 fermentation broth was separated and purified by extraction, column chromatography, and high-performance liquid chromatography. Four monomer compounds with strong herbicidal activity were obtained: p-hydroxyphenyl-2,3-dihydroxypropyl ether, o-hydroxy-3-carbonyl-1-phenylpropanol, 1,8-propanediol o-xylene, and 2-3-dihydroxypropyl propionate. The biological activity verification test indicated that the four monomer compounds could inhibit the germination of wild oat (Avena fatua L.) and canola (Brassica napus L.) seeds. Of the four, compound 3 (1,8-propanediol o-xylene) had obvious inhibitory effects on the germination of A. fatua and B. napus seeds, with inhibition rates of 83.33% and 86.67%, respectively. Therefore, the identification of this monomer compound lays a foundation for the further development of a novel microbial herbicide by directly utilizing it and developing new derivatives with herbicidal functions as lead compounds.","PeriodicalId":23688,"journal":{"name":"Weed Science","volume":"71 1","pages":"39 - 49"},"PeriodicalIF":2.5,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48709363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weed SciencePub Date : 2022-12-15DOI: 10.1017/wsc.2022.64
Fernando J. Pérez-Porras, J. Torres-Sánchez, F. López-Granados, F. Mesas-Carrascosa
{"title":"Early and On-Ground Image-Based Detection of Poppy (Papaver rhoeas) in Wheat Using YOLO Architectures","authors":"Fernando J. Pérez-Porras, J. Torres-Sánchez, F. López-Granados, F. Mesas-Carrascosa","doi":"10.1017/wsc.2022.64","DOIUrl":"https://doi.org/10.1017/wsc.2022.64","url":null,"abstract":"Abstract Poppy (also common poppy or corn poppy; Papaver rhoeas L., PAPRH) is one of the most harmful weeds in winter cereals. Knowing the precise and accurate location of weeds is essential for developing effective site-specific weed management (SSWM) for optimized herbicide use. Among the available tools for weed mapping, deep learning (DL) is used for its accuracy and ability to work in complex scenarios. Crops represent intricate situations for weed detection, as crop residues, occlusion of weeds, or spectral similarities between crop and weed seedlings are frequent. Timely discrimination of weeds is needed, because postemergence herbicides are used just when weeds and crops are at an early growth stage. This study addressed P. rhoeas early detection in wheat (Triticum spp.) by comparing the performance of six DL-based object-detection models focused on the “You Only Look Once” (YOLO) architecture (v3 to v5) using proximal RGB images to train the models. The models were assessed using open-source software, and evaluation offered a range of results for quality of recognition of P. rhoeas as well as computational capacity during the inference process. Of all the models, YOLOv5s performed best in the testing phase (75.3%, 76.2%, and 77% for F1-score, mean average precision, and accuracy, respectively). These results indicated that under real field conditions, DL-based object-detection strategies can identify P. rhoeas at an early stage, providing accurate information for developing SSWM.","PeriodicalId":23688,"journal":{"name":"Weed Science","volume":"71 1","pages":"50 - 58"},"PeriodicalIF":2.5,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45818202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Target-Site and Metabolic Mechanisms of Tolerance to Penoxsulam in Pond Lovegrass (Eragrostis japonica)","authors":"Ying Liu, Hao Wang, Jiapeng Fang, Haitao Gao, Jinyi Chen, Zhen Peng, Liyao Dong","doi":"10.1017/wsc.2022.63","DOIUrl":"https://doi.org/10.1017/wsc.2022.63","url":null,"abstract":"Abstract The identification of herbicide tolerance is essential for effective chemical weed control. According to whole-plant dose–response assays, none of 29 pond lovegrass [Eragrostis japonica (Thunb.) Trin.] populations were sensitive to penoxsulam. The effective dose values of penoxsulam causing 50% inhibition of fresh weight (GR50: 105.14 to 148.78 g ai ha–1) in E. japonica populations were much higher than the label rate of penoxsulam (15 to 30 g ai ha–1) in the field. This confirmed that E. japonica was tolerant to penoxsulam. Eragrostis japonica populations showed 52.83- to 74.76-fold higher tolerance to penoxsulam than susceptible barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.]. The mechanisms of tolerance to penoxsulam in E. japonica were also identified. In vitro activity assays revealed that the penoxsulam concentration required to inhibit 50% of the acetolactate synthase (ALS) activity (IC50) was 12.27-fold higher in E. japonica than in E. crus-galli. However, differences in the ALS gene, previously found to endow target-site resistance in weeds, were not detected in the sequences obtained. Additionally, the expression level of genes encoding ALS in E. japonica was approximately 2-fold higher than in E. crus-galli after penoxsulam treatment. Furthermore, penoxsulam tolerance can be significantly reversed by three cytochrome P450 monooxygenase (CytP450) inhibitors (1-aminobenzotriazole, piperonyl butoxide, and malathion), and the activity of NADPH-dependent cytochrome P450 reductase toward penoxsulam in E. japonica increased significantly (approximately 7-fold higher) compared with that of treated E. crus-galli. Taken together, these results indicate that lower ALS sensitivity, relatively higher ALS expression levels, and stronger metabolism of CytP450s combined to bring about penoxsulam tolerance in E. japonica.","PeriodicalId":23688,"journal":{"name":"Weed Science","volume":"71 1","pages":"29 - 38"},"PeriodicalIF":2.5,"publicationDate":"2022-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42772475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weed SciencePub Date : 2022-11-01DOI: 10.1017/wsc.2022.61
Gino Graziano, P. Tomco, S. Seefeldt, C. Mulder, Zachary C. Redman
{"title":"Herbicides in Unexpected Places: Non-Target Impacts from Tree Root Exudation of Aminopyralid and Triclopyr Following Basal Bark Treatments of Invasive Chokecherry (Prunus padus) in Alaska","authors":"Gino Graziano, P. Tomco, S. Seefeldt, C. Mulder, Zachary C. Redman","doi":"10.1017/wsc.2022.61","DOIUrl":"https://doi.org/10.1017/wsc.2022.61","url":null,"abstract":"Abstract Basal bark treatment of invasive trees is an approach designed to limit damage to non-target vegetation in the vicinity, but non-target injury is still documented. No study of basal bark treatments has examined the release of herbicide residues from roots of treated plants and resulting non-target impacts. Studies were conducted in Alaska interior and coastal boreal forests on basal bark treatments with aminopyralid and triclopyr on active-growth and dormant invasive chokecherry (Prunus padus L.). The study assessed non-target damage and soil herbicide residue using a combination of visual evaluations, bioassays, and soil residue analyses. Non-target damage from herbicide residues were identified in 40% of treatments containing aminopyralid with triclopyr, 60% of treatments containing aminopyralid alone, and 5% of treatments containing only triclopyr. Laboratory studies of aminopyralid treatments to saplings isolated the effects of herbicide exudation from roots, which was found to be significant, and the magnitude was dependent on dose. Herbicide soil residues in field and laboratory experiments were quantified with analytical detection and plant bioassays. Aminopyralid soil residues were identified in 57% of field treatments receiving 8 to 60 ml of herbicide solution (2% ai) and 70% of laboratory treatments receiving 10 µl of herbicide solution (2% to 16% ai). Triclopyr residues were found from one field treatment following dosage with 28 ml of herbicide solution (18.5% ai). Anatomically, plants grown in soils associated with herbicide-treated trees, both in the field and lab, grew less dry mass than non–herbicide treated controls. This study provides the first evidence that root exudation of herbicide following basal bark treatments contributes to non-target damage of adjacent vegetation and to accumulation of soil herbicide residues. This is an important new factor for integrated pest management within basal bark treatment systems and has implications for other herbicide application types such as injections and frill, as well as determining whether root exudation is species or herbicide specific.","PeriodicalId":23688,"journal":{"name":"Weed Science","volume":"70 1","pages":"706 - 714"},"PeriodicalIF":2.5,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47691117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weed SciencePub Date : 2022-11-01DOI: 10.1017/wsc.2022.62
Sandra R. Ethridge, Anna M. Locke, W. Everman, D. Jordan, R. León
{"title":"Crop Physiological Considerations for Combining Variable-Density Planting to Optimize Seed Costs and Weed Suppression","authors":"Sandra R. Ethridge, Anna M. Locke, W. Everman, D. Jordan, R. León","doi":"10.1017/wsc.2022.62","DOIUrl":"https://doi.org/10.1017/wsc.2022.62","url":null,"abstract":"Abstract High crop densities are valuable to increase weed suppression, but growers might be reluctant to implement this practice due to increased seed cost. Because it is also possible to lower planting densities in areas with no or low weed interference risk, the area allocated to each planting density must be optimized considering seed cost and productivity per plant. In this study, the growth and yield of maize (Zea mays L.), cotton (Gossypium hirsutum L.), and soybean [Glycine max (L.) Merr.] were characterized in response to low planting densities and arrangements. The results were used to develop a bioeconomic model to optimize the area devoted to high- and low-density plantings to increase weed suppression without increasing seed cost. Physiological differences seen in each crop varied with the densities tested; however, maize was the only crop that had differences in yield (per area) between densities. When a model to optimize low and high planting densities was used, maize and cotton showed the most plasticity in yield per planted seed (g seed–1) and area of low density to compensate for high-density area unit. Maize grown at 75% planting density compared with the high-planting density (200%) increased yield (g seed–1) by 229%, return by 43%, and profit by 79% while decreasing the low-density area needed to compensate for high-density area. Cotton planted at 25% planting density compared with the 200% planting density increased yield (g seed–1) by 1,099%, return by 46%, and profit by 62% while decreasing the low-density area needed to compensate for high-density area. In contrast, the high morphological plasticity of soybean did not translate into changes in area optimization, as soybean maintained return, profit, and a 1:1 ratio for area compensation. This optimization model could allow for the use of variable planting at large scales to increase weed suppression while minimizing costs to producers.","PeriodicalId":23688,"journal":{"name":"Weed Science","volume":"70 1","pages":"687 - 697"},"PeriodicalIF":2.5,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44496862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}