Veterinarno-meditsinski nauki最新文献_第6页

[Cytological research on the vaginal mucosa in cows with estrous cycle disorders]. 发情周期紊乱奶牛阴道黏膜细胞学研究。

Veterinarno-meditsinski nauki Pub Date : 1987-01-01

G Gospodinov, I Dzhurova, Ia Iosifov, I Petkov

{"title":"[Cytological research on the vaginal mucosa in cows with estrous cycle disorders].","authors":"G Gospodinov, I Dzhurova, Ia Iosifov, I Petkov","doi":"","DOIUrl":"","url":null,"abstract":"Cytologic investigations were carried out of a total of 140 cows with regard to the vaginal mucosa in the event of disturbed functions in the course of the sexual cycle: hypofunction of the ovaries (71 animals), persisting yellow bodies (34 animals), and estral and postestral cases of metrorrhagia (35 animals). Chamov's trichromatic method was employed to stain the smears and Véznik's cytogramme method--to carry out the recordings. It was found that in the case of ovarian hypofunction the mixed type of cell pictures prevailed (47.8 per cent), followed by the estrogenic type (39.4 per cent), and the progesterone type (12.6 per cent). In the case of persisting corpora lutea the mixed type of cell picture prevailed too--64.7 per cent, while the estrogenic and progesterone types were 17.6 per cent. Featuring in the postestral and estral metrorrhagia was the estrogenic type of cell picture (51.4 per cent), while the mixed type accounted for 45.7 per cent of the cases, and the progesterone one--for 2.8 per cent. It is suggested to use the cytovaginal method in combination with the routinely employed clinical and laboratory tests for the overall evaluation of the condition of the genital system in cows.","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 6","pages":"43-7"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14793404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of ochratoxin on the health status of broilers]. [赭曲霉毒素对肉鸡健康状况的影响]。

Veterinarno-meditsinski nauki Pub Date : 1987-01-01

M Aleksandrov, A Dzhurov

引用次数: 0

[Clinical and morphological changes in the hoof sole of large ruminants with inflammatory diseases]. [大型反刍动物炎症性疾病的临床和形态学变化]。

Veterinarno-meditsinski nauki Pub Date : 1987-01-01

J Gutiérrez, A K Angelov, N Bodurov

{"title":"[Clinical and morphological changes in the hoof sole of large ruminants with inflammatory diseases].","authors":"J Gutiérrez, A K Angelov, N Bodurov","doi":"","DOIUrl":"","url":null,"abstract":"Clinical, morphological, and histomorphological investigations were carried out of the changes taking place in the solar matrix in some more common diseases in cows raised in the conditions on the enlarged farms of industrial cattle breeding. With the traumatic aseptic inflammations of the solar matrix the nova and its effect predetermine histologically the various degrees of changes, consisting in circulation disturbances, oedema of the connective tissue, disintegration of the connective tissue bundles, divergence of the collagen fibres, mucinous dystrophy, fibrinoid-necrotic foci, and heterophilic infiltration around the blood vessels and in their wall (arteriitis and periarteriitis). In suppurative inflammations of the solar matrix the process assumes diffuse character with strongly manifested leukocytic infiltration, with the development of dystrophic necrobiotic processes. As a result of these partially or more widely expressed separation (exungulatio) of the solar horn tissue from the matrix sets in. In the initial stage of the traumatic sole ulcer are observed the signs described for an aseptic inflammation. Later on the processes assume a more strongly manifested course, involving the matrix with atrophy and necrosis, separation of the hoof horn from the matrix. With the secondary complication with pathogenic organisms the signs of purulent and necrotic inflammation are seen, resulting in an ulcus defect.","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 5","pages":"80-90"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14751337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Isolation and receptor activity of mammalian lymphocytes]. 哺乳动物淋巴细胞的分离和受体活性

Veterinarno-meditsinski nauki Pub Date : 1987-01-01

R Georgieva, D Stefanov

{"title":"[Isolation and receptor activity of mammalian lymphocytes].","authors":"R Georgieva, D Stefanov","doi":"","DOIUrl":"","url":null,"abstract":"The possibilities of lymphocytes from the blood of pigs, cows, sheep and rabbits, using Lymphoprep, Polysep, and Histopaque were investigated. The conditions given by the producer firms were strictly observed when dealing with the separation media, and in cases when the results were unsatisfactory modifications were made with regard to the velocity and duration of centrifugation in carrying out the separation. The functional state of membrane receptors of lymphocytes was checked by rosette tests with erythrocytes for the detection of T-lymphocytes. It was demonstrated that lymphocytes from pig's blood are successfully isolated with all three separating media, when observing the conditions of manipulation as indicated by producer firms. With ruminants the best results were obtained with Lymphoprep. Polysep is not suitable medium for lymphocyte isolation from cow's blood; Histopaque can be used if the separation is carried out at 1880 r. p. m. for 40 minutes. Good results are obtained with Polysep with sheep's blood when the separation is performed at 1880 r. p. m. for 40 minutes. All three media can be used in isolation of lymphocytes from rabbit's blood. The isolation of lymphocytes with Histopaque must be performed by centrifugation at 1520 r. p. m. for the duration of 40 minutes. The separation media investigated do not harm the membrane receptors of lymphocytes.","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 5","pages":"27-32"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13957887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Phenotypic characteristics of a new modification-restriction system in E. coli]. 一种新的大肠杆菌修饰-限制系统的表型特征

Veterinarno-meditsinski nauki Pub Date : 1987-01-01

B Popovski

引用次数: 0

[Demonstration of a reovirus in helicopter disease of broilers]. [一种呼肠孤病毒在肉鸡直升机病中的演示]。

Veterinarno-meditsinski nauki Pub Date : 1987-01-01

G Gerganov, A Veselinova, G Popov, M Markarian

{"title":"[Demonstration of a reovirus in helicopter disease of broilers].","authors":"G Gerganov, A Veselinova, G Popov, M Markarian","doi":"","DOIUrl":"","url":null,"abstract":"A reovirus was isolated from 5-13-day-old broiler birds with signs of the so-called helicopter disease--one of the forms of the malabsorption syndrome. A number of organs were investigated (trachea, liver, spleen, bursa of Fabricius, proventricular stomach, and tendon sheath of musculus gastrocnemius). The virus was isolated from the proventricular stomach and the tendon sheath in cell cultures of chick embryo kidney as well as in 5-7-day-old chick embryos inoculated in the yolk sac. The remaining organs proved virologically negative. All organs, including the primarily positive ones, were shown to be virologically negative upon the inoculation of chick embryo fibroblast cultures and of chorioallantoic membranes of 10-11-day-old chick embryos. The isolate was identified as an avian reovirus, and was denoted with the initials R-85--on the grounds of its biologic and physico-chemical properties, of its morphology (negative-contrast electron microscopy), and of its morphogenesis (ultrathin electron-microscopic cross sections). Investigated were the histopathologic changes in chick embryos inoculated with the R-85 reovirus and the pathologic changes in the liver of spontaneously affected birds.","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 3","pages":"21-32"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14172638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Superovulation induction in buffalo cows (Bubalus bubalis) with follicle-stimulating hormone (FSH)]. [用促卵泡激素(FSH)诱导水牛超排卵]。

Veterinarno-meditsinski nauki Pub Date : 1987-01-01

Kh Karaivanov, K Vlakhov, M Petrov, D Kacheva, A Aleksiev

引用次数: 0

[Dynamics of the antibiotic resistance of E. coli strains isolated from cows on 2 dairy farms]. [2个奶牛场奶牛分离的大肠杆菌菌株的抗生素耐药性动态分析]。

Veterinarno-meditsinski nauki Pub Date : 1987-01-01

T Kokosharov

引用次数: 0

[Church and monastery medicine during the Bulgarian Renaissance]. [保加利亚文艺复兴时期的教堂和修道院医学]。

Veterinarno-meditsinski nauki Pub Date : 1987-01-01

M Popov

引用次数: 0

[Cultivation and demonstration of the classic swine fever virus]. [经典猪瘟病毒的培养和演示]。

Veterinarno-meditsinski nauki Pub Date : 1987-01-01

N Milev, Ia Peev, P Gergov, M Aleksandrov, V Katevska

{"title":"[Cultivation and demonstration of the classic swine fever virus].","authors":"N Milev, Ia Peev, P Gergov, M Aleksandrov, V Katevska","doi":"","DOIUrl":"","url":null,"abstract":"Experiments were carried out for the cultivation and indication of the swine pestivirus in several continuous and in primary cell lines, using lapinized and field strains of the virus. It was demonstrated that in the various cell cultures the strains used showed varying rates of growth. In PK-15 and pig embryonic kidney cell lines, the field strains and the virulent Vratsa strain replicated with no preliminary adaptation, forming numerous large fluorescent plaques at the 16th to 18th hour. In the same cultures the lapinized strains K and Hudson had more delayed growth, forming double plaques not until the 36th hour. In rabbit kidney primary cultures the virulent K strain only exhibited growth, and up to the 4th hour at that. All results obtained were in agreement with the results from biologic experiments with pigs and rabbits. Experiments were also carried out for the indication of the swine pestivirus in infected lamellae of the cell cultures used, which were subject to additional treatment for 5 min following primary handling with the specific marked serum with the 1:40,000 solution of Evans blue. The infected cells treated by this method showed light green fluorescence of the protoplasm, with a dark nucleus, while the intact cells had tile-red cytoplasm.","PeriodicalId":23492,"journal":{"name":"Veterinarno-meditsinski nauki","volume":"24 4","pages":"21-6"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14434625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0