Veterinaria México OA最新文献

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Evaluation of a rapid immunochromatographic assay for the diagnosis of rabies in regional laboratories of Costa Rica 哥斯达黎加区域实验室快速免疫层析检测狂犬病诊断的评价

Veterinaria México OA Pub Date : 2023-10-23 DOI: 10.22201/fmvz.24486760e.2023.1106

Juan Carlos Alvarado-Fernández, Carolina Salas-Rojas, Josimar Estrella-Morales, Rocío González, Juan Miguel Cordero-Solorzano, Olga Aguilar-Arguedas, Yinnel Soto-Araya, Diana Perez Villalobos, Bernal Leon

{"title":"Evaluation of a rapid immunochromatographic assay for the diagnosis of rabies in regional laboratories of Costa Rica","authors":"Juan Carlos Alvarado-Fernández, Carolina Salas-Rojas, Josimar Estrella-Morales, Rocío González, Juan Miguel Cordero-Solorzano, Olga Aguilar-Arguedas, Yinnel Soto-Araya, Diana Perez Villalobos, Bernal Leon","doi":"10.22201/fmvz.24486760e.2023.1106","DOIUrl":"https://doi.org/10.22201/fmvz.24486760e.2023.1106","url":null,"abstract":"Rabies is a viral, lethal, and zoonotic disease with worldwide distribution, primarily transmitted through bites from infected dogs and bats. The Biosafety Laboratory (LSE-LANASEVE) of the Animal Health Service of Costa Rica (SENASA) serves as the national reference laboratory for diagnosing rabies in humans and animals. Since regional laboratories lack the equipment for the direct fluorescent antibody test (FAT), we evaluated the rapid immunochromatographic diagnostic test (RIDT) from BioNote, employing FAT as a reference, to improve rabies diagnosis. We analyzed 193 brain tissue samples between 2014 and 2019. Out of these, 174 came from species that RIDT has been validated for: bovines (162), dogs (10), and raccoons (2). The rest were from unvalidated species, including horses (7), humans (1), and others. Among the 174 validated samples, 26 bovine samples were positive for both RIDT and FAT. Reviewing all 193 samples, 28 were positive and 165 negative using both methods. Two horse samples presented inconsistencies, being positive on FAT but negative on RIDT; these were subsequently verified as false negatives by RT-PCR. RIDT exhibited a sensitivity of 94 % (CI95, 83.9-102.3), specificity of 100 %, positive predictive value (PPV) of 100 %, and negative predictive value (NPV) of 99 % (CI95, 97.1-100.5). RIDT has demonstrated reliability in quickly diagnosing rabies for validated species. We advise its application in SENASA's regional laboratories for those particular species. If there's uncertainty, samples should be sent to LSE-LANASEVE for FAT or RT-PCR confirmation.","PeriodicalId":23475,"journal":{"name":"Veterinaria México OA","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135461121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential of plant extracts to inhibit egg hatching and migration of Haemonchus contortus larvae 植物提取物对弯曲血蜱幼虫卵孵化和迁徙的抑制作用

Veterinaria México OA Pub Date : 2023-10-20 DOI: 10.22201/fmvz.24486760e.2023.1258

Edgar Aguilar-Urquizo, Erika Mercedes Marcín-Marrufo, Miguel Ángel Magaña-Magaña, Ángel Trinidad Piñeiro-Vázquez, Juan Felipe de Jesús Torres-Acosta, Mateo Itza-Ortiz

{"title":"Potential of plant extracts to inhibit egg hatching and migration of Haemonchus contortus larvae","authors":"Edgar Aguilar-Urquizo, Erika Mercedes Marcín-Marrufo, Miguel Ángel Magaña-Magaña, Ángel Trinidad Piñeiro-Vázquez, Juan Felipe de Jesús Torres-Acosta, Mateo Itza-Ortiz","doi":"10.22201/fmvz.24486760e.2023.1258","DOIUrl":"https://doi.org/10.22201/fmvz.24486760e.2023.1258","url":null,"abstract":"Gastrointestinal parasitosis constitutes important health problems, aggravating throughout the years, due to inadequate use of drugs for its control, causing anthelmintic resistance. The objective of the present study was to evaluate the potential of Azadirachta indica (AZA) and Moringa oleifera (MOR) extracts in inhibiting egg hatching and in vitro migration of Haemonchus contortus larvae. A completely randomized design of experiment was carried out, where 15 treatments and a dimethyl sulfoxide negative control were evaluated: MOR-75, MOR-50, MOR-25, MOR-12.5, and MOR-6.25 mg/mL; AZA-75, AZA-50, AZA-25, AZA-12.5, and AZA-6.25 mg/mL; and thiabendazole (TBZ) TBZ-200, TBZ-100, TBZ-40, TBZ-20, TBZ-10 µg/mL. The extracts did not inhibit egg hatching capacity; however, we observed a motility decrease of up to 100% in L1 larvae. The extracts affected larval migration (P < 0.0020) compared with controls (dimethyl sulfoxide and TBZ), with migration rate inhibition above 65 %. Probit analysis showed that the median effective concentrations were 60.41 and 65.69 mg/mL for M. oleifera and A. indica, respectively. The in vitro results suggest that the water extract of both plants has an anthelmintic action against gastrointestinal nematode larvae.","PeriodicalId":23475,"journal":{"name":"Veterinaria México OA","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135665650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of ivermectin and moxidectin against cyathostomins in four horse breeding farms in Mexico 伊维菌素和莫西菌素在墨西哥四个马养殖场对cyathostomins的有效性

Veterinaria México OA Pub Date : 2023-09-13 DOI: 10.22201/fmvz.24486760e.2023.1192

Cintli Martínez Ortiz de Montellano, Roger Ivan Rodriguez-Vivas, José Alberto Rosado-Aguilar, Guadalupe Galicia-Velázquez

{"title":"Effectiveness of ivermectin and moxidectin against cyathostomins in four horse breeding farms in Mexico","authors":"Cintli Martínez Ortiz de Montellano, Roger Ivan Rodriguez-Vivas, José Alberto Rosado-Aguilar, Guadalupe Galicia-Velázquez","doi":"10.22201/fmvz.24486760e.2023.1192","DOIUrl":"https://doi.org/10.22201/fmvz.24486760e.2023.1192","url":null,"abstract":"This study aimed to evaluate the effectiveness of oral ivermectin and moxidectin against natural cyathostomin infection in four horse farms located in the central regions of Mexico. 445 horses of the Warmblood (145), Thoroughbreds (100), and Quarter Horses (200) breeds, aged between 6 months and 27 years, were used. Data on horses and parasite control methods were collected through interviews with farm owners and veterinarians. Using the McMaster technique, fecal samples were processed from all 445 horses, 180 of which were positive for cyathostomins. On each farm, a selection was made of 45 animals meeting the criteria of a Fecal Egg Count Reduction Test yielding results exceeding 150 eggs per gram of strongylid-type nematodes. Subsequently, three separate experimental groups were formed for each farm, each consisting of 15 horses The first group was treated with oral ivermectin 1.87 %; the second group with oral moxidectin 2 %; and the third was the non-treatment control group. Coprocultures were also performed to identify the presence of nematode species. The data obtained were analyzed with RESO.exe©️. Three of the four farms achieved a 100 % reduction in eggs per gram with both macrocyclic lactones. One farm achieved 93 % reduction with ivermectin and 87 % with moxidectin. This study demonstrates that macrocyclic lactones effectively reduce cyathostomins in three of the four farms studied. The results suggest potential cyathostomin resistance to macrocyclic lactones, particularly moxidectin, on one farm. Given these findings, sustainable parasite management is required on horse breeding farms in Mexico.","PeriodicalId":23475,"journal":{"name":"Veterinaria México OA","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135783074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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