发布求助
文献互助 智能选刊 最新文献

Ukrainskii biokhimicheskii zhurnal最新文献

筛选
英文 中文
[Isolation of tyrosyl-tRNA-synthetase from Thermus thermophilus HB-27]. [从嗜热热菌HB-27中分离酪氨酸- trna合成酶]。
Ukrainskii biokhimicheskii zhurnal Pub Date : 1989-09-20 DOI: 10.7124/BC.0000EA
A. D. Iaremchuk, M. Tukalo, S. Egorova, A. Konovalenko, G. Matsuka
{"title":"[Isolation of tyrosyl-tRNA-synthetase from Thermus thermophilus HB-27].","authors":"A. D. Iaremchuk, M. Tukalo, S. Egorova, A. Konovalenko, G. Matsuka","doi":"10.7124/BC.0000EA","DOIUrl":"https://doi.org/10.7124/BC.0000EA","url":null,"abstract":"A method for isolating tyrosyl-tRNA synthetase from Thermus thermophilus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparin-sepharose and hydrophobic chromatography on Toyopearl HW-65. The yield of the purified enzyme was 1.6 mg per 1 kg of T. thermophilus cells. The enzyme is a dimer protein of the alpha 2 type with molecular weight of 100 kDa.","PeriodicalId":23403,"journal":{"name":"Ukrainskii biokhimicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74077327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
[Entrapping of hydrophobized plague capsular antigen into large unilamellar liposomes]. [将疏水鼠疫囊抗原包埋到大的单层脂质体中]。
Ukrainskii biokhimicheskii zhurnal Pub Date : 1989-01-01 DOI: 10.21236/ada241775
V. Zakrevskiĭ, N. G. Plekhanova, V. I. Smirnova
{"title":"[Entrapping of hydrophobized plague capsular antigen into large unilamellar liposomes].","authors":"V. Zakrevskiĭ, N. G. Plekhanova, V. I. Smirnova","doi":"10.21236/ada241775","DOIUrl":"https://doi.org/10.21236/ada241775","url":null,"abstract":"The entrapping of a plague capsular antigen into the liposomes, prepared by the \"reverse phase\" evaporating method, was investigated. Both the native antigen and the antigen modified by palmitoylchloride were used. The entrapping of the modified antigen into the liposomes was greater than that of the native one: 46.1 +/- 5.4% and 3.7 +/- 2.7%, respectively. The highest content of the antigen on the membrane surface was observed when using palmitoylized protein and liposomes with egg lecithin, cholesterol and dicethylphosphate in the molar ratio 7:2:1.","PeriodicalId":23403,"journal":{"name":"Ukrainskii biokhimicheskii zhurnal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79190557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信