[从嗜热热菌HB-27中分离酪氨酸- trna合成酶]。

Ukrainskii biokhimicheskii zhurnal Pub Date : 1989-09-20 DOI:10.7124/BC.0000EA

A. D. Iaremchuk, M. Tukalo, S. Egorova, A. Konovalenko, G. Matsuka

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引用次数: 6

摘要

介绍了一种从嗜热热菌中分离酪氨酸- trna合成酶的方法，包括硫酸铵分离、DEAE-sepharose、羟基磷灰石、肝素-sepharose层析和Toyopearl HW-65疏水层析。纯化酶的产率为1.6 mg / 1kg嗜热t细胞。该酶为α 2型二聚体蛋白，分子量为100 kDa。

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[Isolation of tyrosyl-tRNA-synthetase from Thermus thermophilus HB-27].

A method for isolating tyrosyl-tRNA synthetase from Thermus thermophilus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparin-sepharose and hydrophobic chromatography on Toyopearl HW-65. The yield of the purified enzyme was 1.6 mg per 1 kg of T. thermophilus cells. The enzyme is a dimer protein of the alpha 2 type with molecular weight of 100 kDa.

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Ukrainskii biokhimicheskii zhurnal

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