Research Journal of Applied Biotechnology最新文献

An innovative extracellular polysaccharide produced by Egyptian Marine Bacillus licheniformis, and its applications as antibacterial agent. 埃及海洋地衣芽孢杆菌生产的一种新型胞外多糖及其抗菌应用。

Research Journal of Applied Biotechnology Pub Date : 2023-07-30 DOI: 10.21608/rjab.2023.198462.1027

Sokara Ali, M. Amer, H. Hamza, R. Abbas

{"title":"An innovative extracellular polysaccharide produced by Egyptian Marine Bacillus licheniformis, and its applications as antibacterial agent.","authors":"Sokara Ali, M. Amer, H. Hamza, R. Abbas","doi":"10.21608/rjab.2023.198462.1027","DOIUrl":"https://doi.org/10.21608/rjab.2023.198462.1027","url":null,"abstract":"Twenty-two marine bacterial isolates were collected from the Eastern Harbor (29885 E longitude and 31205 N latitude) located at Alexandria, Egypt selected to produce exopolysaccharides, using the Shene extraction method in 2008. The most potent marine bacterium was identified as Bacillus licheniformis SMRH1 , accession number ON777836 by using 16s rRNA as a molecular identification technique. According to the chemical analysis, the obtained polysaccharide content is estimated by using Fourier Transform Infrared spectra FTIR spectrum of the extracted exopolysaccharide from Bacillus licheniformis which showed that it contains νOH at 3420cm -1 , νC-H aliphatic at 2952cm -1 , νC=O in range 1660-1630cm -1 and Nuclear magnetic resonance spectra NMR showed signals δ’ S ppm at 1.22 (s, -CH 3 ), 2.07 (s, -CH 2 chain ) and 2.35(m, -CH 2 ) and Scanning electron microscope SEM which confirmed the amorphus shaped uneven texture polysaccharide at higher magnification of ×5000. The exopolysaccharide that was generated has antibacterial activity by forming a free hollow zone around it against some gram positive and gram-negative bacteria according to disc diffusion method. The innovatively produced exopolysaccharide also has anticoagulant activity. A recent study proved that the generated exopolysaccharide induced β-hemolysis. The high yield of exopolysaccharide was “0.7 g/l” by using different culture media such as nutrient broth, peptone water, and king ̓s B broth.","PeriodicalId":233013,"journal":{"name":"Research Journal of Applied Biotechnology","volume":"190 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131581962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between some H5 commercial vaccines and the highly pathogenic H5N8 avian influenza virus that is recently circulating in Egypt 一些H5商业疫苗与最近在埃及流行的高致病性H5N8禽流感病毒之间的关系

Research Journal of Applied Biotechnology Pub Date : 2023-07-07 DOI: 10.21608/rjab.2023.208565.1036

N. Bedair, Moustafa A. Sakr, O. Khamiss, A. Mostafa

引用次数: 0

Isolation of Lactobacillus HBUAS56719 from Black Raspberry Leaves and its in-vitro antidiabetic activity 黑树莓叶中HBUAS56719乳杆菌的分离及体外抗糖尿病活性研究

Research Journal of Applied Biotechnology Pub Date : 2023-03-01 DOI: 10.21608/rjab.2023.175866.1022

Mohammed Abdalla, Amira Kasser, A. B. Mohammed

引用次数: 0

Screening of Natural β-lactamase Inhibitors (BLIPs) from Two Newly Isolated Egyptian Streptomyces strains 两株新分离的埃及链霉菌天然β-内酰胺酶抑制剂的筛选

Research Journal of Applied Biotechnology Pub Date : 2022-12-30 DOI: 10.21608/rjab.2022.266762

Borhan Eltabey, N. Sorour, H. Hamza, R. Abbas

引用次数: 0

Antibacterial activity of multidrug resistance Escherichia coli by some natural products 几种天然产物对多重耐药大肠杆菌的抑菌活性研究

Research Journal of Applied Biotechnology Pub Date : 2022-12-03 DOI: 10.21608/rjab.2022.170948.1021

‪mohamed agami‬‏

引用次数: 1

Could hsa-miR-29c and hsa-miR-30c be Used as Biomarkers for Day 3 Healthy Human Embryos in In-Vitro Fertilization? hsa-miR-29c和hsa-miR-30c可以作为体外受精第3天健康人类胚胎的生物标志物吗?

Research Journal of Applied Biotechnology Pub Date : 2022-12-01 DOI: 10.21608/rjab.2022.138907.1014

Samer Al-Sammany, Y. Ali, A. Awad, A. Elmaghraby, O. Khamiss

{"title":"Could hsa-miR-29c and hsa-miR-30c be Used as Biomarkers for Day 3 Healthy Human Embryos in In-Vitro Fertilization?","authors":"Samer Al-Sammany, Y. Ali, A. Awad, A. Elmaghraby, O. Khamiss","doi":"10.21608/rjab.2022.138907.1014","DOIUrl":"https://doi.org/10.21608/rjab.2022.138907.1014","url":null,"abstract":"Embryo’s quality is closely linked to miRNA regulation, which plays a critical role in gene expression and protein synthesis. In this prospective cohort observational study, extracellular miRNAs were tested using the miRCURY Locked Nucleic Acid (LNA) system, which used culture media from twenty-two singly transferred embryos. Study samples were collected on day three and embryos were transferred on day five. Based on earlier publications, hsa-miR-29c-3p and hsa-miR-30c-5p, among other miRNAs (not published), were chosen and assessed. The negative-implantation group had higher expression of hsa-miR-30c-5p regarded as statistically significant (P.value <0.001) than the other tested miRNAs. Contrary, there was no significant link between pregnancy outcome and expressed hsa-miR-29c-3p. Study findings, in comparison with findings from other reports, reveal the absence of hsa-miR-29c-3p and hsa-miR30c-5p from day 3 implanting embryos in contrast with day 5 where they are abundant, suggesting specificity to the embryo’s development from cleavage to morula, blastocyst, and implantation stages. These findings could open the gate toward potential biomarkers for embryo development and implantation outcome.","PeriodicalId":233013,"journal":{"name":"Research Journal of Applied Biotechnology","volume":"198 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121628538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, characterization, DNA binding of nano Mn2+and Cu2+complexes 纳米Mn2+和Cu2+配合物的合成、表征和DNA结合

Research Journal of Applied Biotechnology Pub Date : 2022-07-01 DOI: 10.21608/rjab.2022.292262

N. Rizk, S. Aly, M. Khidr

引用次数: 0

Expression Analysis of CCL27: A Sensitive biomarker in distinguishing between psoriasis and atopic dermatitis 鉴别银屑病和特应性皮炎的敏感生物标志物CCL27的表达分析

Research Journal of Applied Biotechnology Pub Date : 2022-07-01 DOI: 10.21608/rjab.2022.288248

Ayman Elrefaei, M. ElHamshary, H. Ismail, Mahmoud I. Nasr, G. Nasr

引用次数: 0

Secreted factors of human Wharton’s Jelly stem cells inhibit growth and biofilm formation of Staphylococcus aureus in vitro 人华顿氏水母干细胞分泌因子体外抑制金黄色葡萄球菌生长和生物膜形成

Research Journal of Applied Biotechnology Pub Date : 2021-12-01 DOI: 10.21608/rjab.2021.232755

{"title":"Secreted factors of human Wharton’s Jelly stem cells inhibit growth and biofilm formation of Staphylococcus aureus in vitro","authors":"","doi":"10.21608/rjab.2021.232755","DOIUrl":"https://doi.org/10.21608/rjab.2021.232755","url":null,"abstract":"Staphylococcus aureus is causing high rates of morbidity and mortality in human. Stem cells secreted factors are safe and efficient alternative to stem cells in stem cells-based therapies. In this study, this study aimed to evaluate the antibacterial activity of factors secreted by human Wharton’s jelly mesenchymal stem cells secretome (hWJ-MSCs) against Staphylococcus aureus, hWJ-MSCs were cultured and collected their secreted factors to be used for In vitro antibacterial screening against Staphylococcus aureus . Antibacterial activity, antibiofilm, and minimum inhibitory concentrations (MIC) of hWJ-MSC-S against Staphylococcus aureus were determined. The present data showed that hWJ-MSC secreted factors significantly inhibit the growth of two clinical isolate of Staphylococcus aureus. hWJ-MSC-secreted factors reduced the growth of Staphylococcus aureus by mor than 87% compared to un-treated control. MIC values were 4.68 µg/ml and 1.17 µg/ml for Staphylococcus aureus 76, and Staphylococcus aureus 105, respectively. The finding from this work could be utilized to develop an effective therapeutic approach to treat Staphylococcus aureus infection .","PeriodicalId":233013,"journal":{"name":"Research Journal of Applied Biotechnology","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121375089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective targeting of CagA and autophagy-related Atg12 as a potential therapeutic approach for Helicobacter Pylori infection 有效靶向CagA和自噬相关的Atg12作为幽门螺杆菌感染的潜在治疗方法

Research Journal of Applied Biotechnology Pub Date : 2021-11-15 DOI: 10.21608/rjab.2021.85192.1007

Ahmad Salah, A. Rashed, N. Abbas, K. Bassiouny, Hany Khalil

{"title":"Effective targeting of CagA and autophagy-related Atg12 as a potential therapeutic approach for Helicobacter Pylori infection","authors":"Ahmad Salah, A. Rashed, N. Abbas, K. Bassiouny, Hany Khalil","doi":"10.21608/rjab.2021.85192.1007","DOIUrl":"https://doi.org/10.21608/rjab.2021.85192.1007","url":null,"abstract":"Helicopter pylori (H. Pylori) begins its long-term infection in the stomach, where the immune system responds with local neutrophil, lymphocyte, and macrophage infiltration. H pylori infection is known to cause stomach ulcers, but scientists are still largely in the dark about this critical area of health. We transfected HeLa cells with specific siRNA antagonist bacterial CagA gene or siRNA antagonist autophagy-related Atg12, and then H. Pylori reproduction was monitored. We also looked at proinflammatory cytokine secretion from treated cells to see if there was a link between bacterial CagA, host autophagy, and inflammatory events like tumor necrosis factor-alpha (TNF-) and interleukin-6 (IL6). When compared to control-transfected cells, our findings revealed previously unnoticed changes in cell morphology, number of survived cells, and lactate dehydrogenase (LDH) production after transfection with siRNA targeting CagA or Atg12. Furthermore, targeting CagA effector and Atg12 successfully disrupts H. pylori reproduction in infected cells, as measured by quantitative real-time PCR of bacterial 16S ribosomal RNA (16S rRNA) (q-RT-PCR). Furthermore, CagA downregulation causes an obvious decrease in Raf-1 gene expression, which is an indicator of Megaton-Activated Protein Kinase (MAPK) signaling in infected cells. Finally, IL-6 and TNFproduction was mediated in siRNAtransfected cells that attacked either CagA or Atg12 effectors. In conclusion, these findings suggest that","PeriodicalId":233013,"journal":{"name":"Research Journal of Applied Biotechnology","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134282521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0