Jaringan Laboratorium Medis最新文献

{"title":"Gambaran Morfologi Eritrosit Mahasiswi saat Menstruasi","authors":"Muhammad Syamsul Arif Setiyo Negoro, Glori Septilia","doi":"10.31983/jlm.v6i1.9926","DOIUrl":"https://doi.org/10.31983/jlm.v6i1.9926","url":null,"abstract":"College students when entering the early adult phase have experienced puberty which is marked by menstruation. The duration of menstruation between one woman and another varies greatly so that the volume of blood released also varies. Excessive blood loss can reduce iron (Fe) levels in the body of female students and can affect erythrocyte morphology. Researchers conducted this study with the aim of knowing the description of erythrocyte morphology of female college students during menstruation. The research conducted is included in the type of descriptive research with observational design. The population was d3 TLM class 12 students with a sample size of 20 students who were experiencing menstruation. The sampling technique used incidental sampling. The results described the morphology of erythrocytes in female students of DIII Medical Laboratory Technology Batch XII Poltekkes Kemenkes Semarang during menstruation on the 4th day based on aspects of assessment of erythrocyte size, namely 7 people (35%) included in the microcytic category and 13 people (65%) included in the normocytic category. Based on the aspect of erythrocyte staining, 7 people (35%) were included in the hypochromic category and 13 people (65%) were included in the normochromic category. While based on the aspect of assessing the shape of erythrocytes, all 20 respondents (100%) were included in the mild poikilocytosis category. The conclusion that can be drawn from this study is the picture of erythrocyte morphology of DIII Medical Laboratory Technology Batch XII Poltekkes Kemenkes Semarang students during menstruation on the 4th day, out of 20 respondents as many as 7 people (35%) showed hypochromic microcytic erythrocyte morphological abnormalities with mild poikilocytosis and 13 people (65%) showed normocytic normochromic erythrocyte morphological abnormalities with mild poikilocytosis. It is recommended to increase the consumption of vegetables and foods that increase the formation of red blood cells during menstruation.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"64 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141035418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in Vitamin D3 Levels in Pulmonary Tuberculosis Patients Before and After One Month of Anti-Tuberculosis Drug Therapy","authors":"Rachmad Bayu Kuncara, Dewina Zumrotin Najiah","doi":"10.31983/jlm.v6i1.11379","DOIUrl":"https://doi.org/10.31983/jlm.v6i1.11379","url":null,"abstract":"Pulmonary tuberculosis is an infectious disease caused by infection with the bacteria Mycobacterium tuberculosis. Patients diagnosed with pulmonary tuberculosis will receive Anti-Tuberculosis Drug (OAT) therapy for six months. In the first two months or what is called the intensive phase, the patient will be given a combination of OAT in the form of rifampicin, isoniazid, ethambutol and pyrazinamide. Several types of anti-tuberculosis drugs have the potential to affect vitamin D3 metabolism, either by interfering with absorption, metabolism or changing the action of vitamin D3. The long-term treatment process for pulmonary tuberculosis also results in a decrease in vitamin D3 levels in the blood. This study aims to determine the difference in vitamin D3 levels in pulmonary tuberculosis sufferers before and after one month of anti-tuberculosis drug therapy. This type of research is descriptive observational with a prospective cohort design without comparison. The total sample was 16 newly diagnosed pulmonary tuberculosis sufferers at the Bangetayu, Kedungmundu and Tlogosari Wetan Health Centers, Semarang City. Vitamin D3 levels in serum samples were examined using the ELISA method. The mean vitamin D3 level before OAT therapy was 19.00 ± 10.45 ng/mL, the level decreased after OAT therapy, namely 18.51 ± 4.74 ng/mL. These results indicate that administration of anti-tuberculosis drugs may have an effect on vitamin levels.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"8 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141055784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Differences in Hematocrit Values in Patients with Stage 1 and Stage 2 Hypertension","authors":"Supriati Wila Djami, Marni Tangkelagi, Meliance Bria, M. Loaloka","doi":"10.31983/jlm.v6i1.10594","DOIUrl":"https://doi.org/10.31983/jlm.v6i1.10594","url":null,"abstract":"Blood pressure is the pressure exerted by blood against the walls of blood vessels and is generated by the pressure of blood against the walls of arteries when the blood is pumped from the heart to the tissues. The amount of pressure varies depending on the blood vessels and heart rate. Hypertension is a non-communicable disease that triggers health problems that occur throughout the world. Kupang Regency is one of the districts with the highest cases of hypertension in NTT province with 1,582 cases. Hypertension is closely related to hemoglobin levels, hematocrit values and erythrocyte counts. There is a significant positive relationship between hematocrit values and diastolic blood pressure. Increased blood pressure has a significant positive relationship between hemoglobin levels, hematocrit values and erythrocyte counts. Apart from that, factors that influence hypertension with hemoglobin levels, hematocrit values and erythrocyte counts are age, gender, circulating fibrinogen and red blood cell aggregation. The aim of this research is to determine the difference in hematocrit values in sufferers of grade one and degree two hypertension. This research uses a cross-sectional analytical research design based on primary data originating from examination results in Bone Village, Nekamese District, Kupang Regency in April 2023 which meets the research sample criteria. Sampling for this research was determined using the Purposive Sampling technique. The research respondents were 30 hypertension sufferers in Bone Village, Nekamese District, Kupang Regency. Research on blood pressure and hematocrit values was carried out on all respondents. The results showed that hypertension sufferers consisted of 5 (17%) men and 25 (83%) women with the highest age of hypertension sufferers being > 56 years old, 24 people (80%). There were 9 (30%) people suffering from grade 1 hypertension and 21 (70%) people suffering from degree 2 hypertension with the hematocrit value of most hypertensive sufferers still within normal limits, 18 (60%) and low hematocrit values as many as 12 (40%). The results of this study were processed using SPSS software with Mann-Whitney Test analysis and presented descriptively regarding the analysis of hematocrit values in sufferers of degree one and degree two hypertension. The results of statistical analysis show p-value > 0.05, which means there is no significant difference between variables and vice versa. The results of the study showed that there was no difference in hematocrit values in sufferers of degree one and degree two hypertension with a value of p=0.295. In this study, hypertension sufferers generally had normal hematocrit values, but in second-degree hypertension sufferers the hematocrit values tended to be low.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"33 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141050796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effectiveness of Ylang Ylang Oil (Cananga Odorata) as a Deparaffinizing Agent in Hematoxylin-Eosin Staining","authors":"Gela Setya Ayu Putri, Dina Diyanah, Arya Iswara","doi":"10.31983/jlm.v6i1.10824","DOIUrl":"https://doi.org/10.31983/jlm.v6i1.10824","url":null,"abstract":"Histological staining usually requires deparaffinization before starting the staining process. Deparaffinization is a paraffin removal step that often uses a xylol solution. Deparaffinization with xylol has disadvantages, including being toxic, harmful to the human body, and hazardous to the environment. A safer alternative to xylol is required. Ylang-ylang oil, which contains β-caryophyllene, is non-polar and can be used as a substitute for xylol. This study aimed to compare the staining quality of guinea pig liver tissue slides with and without heating as a deparaffinization agent in hematoxylin-eosin (HE) staining. The research set up a quasi-experimental approach using samples of guinea pig liver tissue slides. The sample size was calculated using the Federer formula (n-1) (t-1) ≥ 15, resulting in a total sample of 27 slides divided into three treatment groups: xylol, ylang-ylang oil with and without heating. The Kruskal-Wallis test was used to examine the data. The quality staining of guinea pig liver slides deparaffinized with xylol was 100% good, and deparaffinization of ylang-ylang oil with heating was 100% good, whereas deparaffinization with ylang-ylang oil without heating was 11.1% less good, and the quality was good with an 88.9%. The normality test indicated that the data were not normally distributed (p0.05). The Kruskal-Wallis test was then used.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141026788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Desain Primer untuk Deteksi Gen Diphtheria Toxin Repressor (dtxR) sebagai Biomarker Bakteri Corynebacterium diphtheriae Menggunakan In Silico PCR","authors":"Hilari Rio Rosa Nastiti, Rachmad Bayu Kuncara","doi":"10.31983/jlm.v5i2.10588","DOIUrl":"https://doi.org/10.31983/jlm.v5i2.10588","url":null,"abstract":"Corynebacterium diphtheriae is the bacteria that causes diphtheria. The virulence factor of C. diphtheriae comes from the bacteria's ability to produce bacterial toxins. Toxin production is regulated by a set of genes called tox/dtx genes and is regulated by the dtxR gene. The aim of this study was to design primers used to evaluate the dtxR gene using bacterial DNA sequences. This research is experimental research with a literature study approach using the In silico Polymerase Chain Reaction (PCR), NCBI (National Center for Biotechnology Information), Primer3Plus, and Oligo Calculator applications. The sample obtained from genbank NCBI was C. diphtheriae dtxR gene M80337.1. In silico PCR examination was carried out using newly designed primers from Primer3Plus with 50 genomic DNA of Corynebacterium spp. taken from the In silico PCR database. The dtxR primer pair: '5-ACAGTTAGCCAAACCGTTGC-3' and 5'-TGCGTTCAACTTCGTCACTC-3' can produce a single DNA amplicon measuring 226 bp specifically for C. diphtheria types and no amplicon bands were generated from other Corynebacterium genomes. Based on the study results, this pair of specific primers can be used for in vitro PCR testing and can be used to develop rapid detection of diphtheria.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139303138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analisis Kadar Timbal (Pb) Darah dan Malondialdehid (MDA) Pada Manusia Silver di Kota Semarang","authors":"U. Rosidah, Ichsan Hadipranoto","doi":"10.31983/jlm.v5i2.9519","DOIUrl":"https://doi.org/10.31983/jlm.v5i2.9519","url":null,"abstract":"The danger of lead in paint that is coated on the human body, silver can enter through the skin and will interact with proteins, DNA, RNA and oxidative stress. Formation of Reactive Oxygen Species (ROS) by lead can cause oxidative stress and is characterized by an increase in the enzymes catalase, malondialdehyde (MDA) and Oxidized glutathione (GSSG). The aim of this study was to analyze blood lead levels against Malondealdehyde (MDA) in silver humans in Semarang City. This study used an observational analytic study with a cross sectional approach to analyze the effect of lead levels on Malondialdehyde levels in silver humans in Semarang City using a t test to see differences in results based on length of work, length of exposure and age on lead levels, and the effect of lead on MDA. The results showed that from 20 human samples, the lowest lead level was 23.9 g/dl and the highest was 61.9 g/dl with an average blood lead level of 37.3 g/dl with an average blood lead level of 37.3 g/dl, and the lowest MDA level was 0.4 mg/L and the highest level is 1.74 mg/L with an average of 1.2 mg/L. Based on the data obtained from the t test, it shows a significant value (P-Value) of 0.610 which means 0.05, this indicates that the decision taken is to reject H1 which means that there is no effect between the results of lead levels on the results of MDA levels.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123969344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between Total Cholesterol Levels and Blood Pressure in Bangetayu Adult, Semarang City","authors":"Ririh Jatmi Wikandari, Devi Etivia Purlinda, Burhanudin Ichsan","doi":"10.31983/jlm.v5i1.9379","DOIUrl":"https://doi.org/10.31983/jlm.v5i1.9379","url":null,"abstract":"Non-communicable diseases (NCDs) are the largest contributor to death in the world. The prevalence of NCDs tends to increase. Hypertension is one of the non-communicable diseases with the largest proportion in Semarang. High cholesterol levels will accumulate in  the arteries, causing the arteries to stiffen, so the heart tries hard to pump blood through these blood vessels, and can cause blood pressure to rise (hypertension). Some risk factors that can increase blood pressure, such as high salt intake, overweight or obesity, alcohol consumption, lack of physical activity, stress and smoking. Lack of physical activity can increase cholesterol levels in the body which is a risk factor for cardiovascular disease (coroner's heart disease dan stroke). Age affects the occurrence of hypertension. With age the risk of developing hypertension is greater. The purpose of the study was to determine the relationship between cholesterol and blood pressure in adults in Bangetayu, Semarang City. This study uses a cross-sectional approach. Research samples are in the community of  Bangetayu Kota Semarang. Research Tools and Materials include lanceolate, alcohol swab, cholesterol strips, digital sphygmomanometers, POCT cholesterol and capillary blood.  Research data obtained from the results of checking cholesterol levels and blood pressure. The results of this study were processed using SPSS 20 software with data analysis using Chi Square (α=5%) and presented descriptively about the relationship of cholesterol levels with blood pressure in adults. The results of statistical analysis show a p-value 0.05 which means that there is no significant relationship between variables and vice versa. The results showed that there was no relationship between blood pressure and cholesterol.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122779027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gestational Diabetes Mellitus (GDM) Screening In Pregnant Women and Diabetes in The Elderly Getasan","authors":"W. Widodo, A. Riyadi, S. Y. D. Widiyanto, Aulia Qurotul Ayuni","doi":"10.31983/jlm.v5i1.9661","DOIUrl":"https://doi.org/10.31983/jlm.v5i1.9661","url":null,"abstract":"Type 1 and type 2 diabetes are influenced by genetic and environmental factors causing a decrease in β cell function resulting in hyperglycemia. Patients with hyperglycemia are at risk for chronic complications. The elderly have the potential to suffer from diabetes mellitus due to the age factor and the decreased ability of the body's metabolism. Whereas in pregnant women any hyperglycemia is considered gestational diabetes mellitus (GDM), regardless of diabetes occurring before or during the current pregnancy GDM is diabetes diagnosed in the second trimester or later. This study aims to map cases of diabetes and gestational diabetes. This research is a non-experimental study with a survey method approach without intervention that is descriptive analytic research results of 48 people with a total of 14 men and 5 pregnant women out of a total of 34 female respondents. Examination results for supporting examinations obtained the results of 8 glucose samples with levels 200 mg/dl and those who had diabetes mellitus based on HbA1c examination were 6 people aged 45-65 years and as many as 3 people aged over 65 years. Respondents who had diabetes mellitus were 2 men and 4 women and none of the pregnant women had gestational diabetes. This research has an impact on the people who take part in this activity, one of which is getting education on how to maintain a healthy quality of life in old age and for those who enter the diabetes mellitus screening group, it is continued in the process of care and assistance by the Getasan Health Center.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123995642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kejadian Toksoplasmosis pada Wanita Hamil Pemelihara Kucing dan Wanita Hamil Bukan Pemelihara Kucing di Puskesmas Tlogosari Wetan Kota Semarang","authors":"Dina Afrianti, Adita Puspitasari Swastya Putri, W. Wahyudi","doi":"10.31983/jlm.v5i1.9396","DOIUrl":"https://doi.org/10.31983/jlm.v5i1.9396","url":null,"abstract":"Toxoplasma gondii (T. gondii) is a widespread zoonotic pathogen worldwide. This parasite grows inside the body of a cat as definitive host. Cats play an important role in the epidemiology of T. gondii because they are the only animal capable to shed oocysts which can withstand outside environment. Nowadays, transmission of T. gondii infection from cat to human increases in particular areas. This study aims to identify the incidence of toxoplasmosis in pregnant women Raise Cats and Not Raise Cats in Tlogosari Wetan Health Center Semarang City. This study was an descriptive observational research with case control design in two populations. Subjects are chosen based on certain criteria, This study was done to 25 respondents as pregnant women raise cats and 25 respondents who weren’t. The results obtained from serological analysis on 50 blood serum samples consisting of 25 blood serum samples of pregnant women raise cats with positive IgG antibody as many as 6 serum samples (24%) and 25 blood serum samples of pregnant women who are not raise cats obtained positive IgG antibody in 2 samples serum (8%). The total percentage of toxoplasmosis was 16% from 50 serum samples. It is necessary to carry out a large-scale serological survey to determine the prevalence of toxoplasmosis in pregnant women in Semarang City and necessary monitoring and treatment of pregnant women infected with Toxoplasma gondii.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130690735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHOD-PAP Method Total Cholesterol Examination Validation on Samples Stored for 1 Week In 4-8°C Temperature","authors":"J. Nabilla, Nabilatul Ulya, Rieke Rofikoh Ardianty, Sabrina Dela Agiska, Fatkhatin Nadifah, N. Qomariyah","doi":"10.31983/jlm.v5i1.8690","DOIUrl":"https://doi.org/10.31983/jlm.v5i1.8690","url":null,"abstract":"High total cholesterol levels cause various diseases, especially those related to the heart  and blood vessels. Total cholesterol test uses serum as a sample and must be analyzed within one hour after sampling.However, in the field sometimes there are inspection delays.Some studies have proven that serum stored for one week at a temperature of 4-8°C has different results in total cholesterol examination. For this reason, this study aims to determine the validity of total cholesterol examination using the CHOD-PAP method of serum samples stored for one week at a temperature of 4o-8oC. Validation test based on eight parameters, there are precision, accuracy, linearity, reportable range,limit detection, quantitation limit,  recovery, and interference. Only the recovery test was not accepted from eight parameters. Based on the Kruskal Wallis test, the sig value is 0.172 0.05, it can be stated that there is no difference in serum storage time for one week at a temperature of 4-8℃ on total cholesterol levels using CHOD-PAP method.","PeriodicalId":232833,"journal":{"name":"Jaringan Laboratorium Medis","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126313958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}