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EFFICIENCY OF THREE SYSTEMS OF NUCLEIC ACID EXTRACTION FOR THE DIAGNOSIS OF PRDC-COMPLEX PATHOGENS 三种核酸提取系统对prdc复合体病原体的诊断效果
Epizootology Immunobiology Pharmacology Sanitation Pub Date : 2022-05-18 DOI: 10.47612/2224-168x-2022-1-56-60
E. Krasnikova, M. M. Misteyko, O. V. Malchik, A. Lysenko
{"title":"EFFICIENCY OF THREE SYSTEMS OF NUCLEIC ACID EXTRACTION FOR THE DIAGNOSIS OF PRDC-COMPLEX PATHOGENS","authors":"E. Krasnikova, M. M. Misteyko, O. V. Malchik, A. Lysenko","doi":"10.47612/2224-168x-2022-1-56-60","DOIUrl":"https://doi.org/10.47612/2224-168x-2022-1-56-60","url":null,"abstract":"The article presents data on the qualitative and quantitative assessment of DNA/RNA obtained using three different isolation kits. Comparative results of PCR with primers for three bacterial and two viral infections are presented. A comparative assessment was carried out.","PeriodicalId":232446,"journal":{"name":"Epizootology Immunobiology Pharmacology Sanitation","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129397722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
FACTORS INFLUENCING THE BIOLOGICAL AND MORPHOMETRIC PARAMETERS OF HETERAKIS GALLINARUM EGGS (GMELIN, 1790) 影响异种鸡卵生物学和形态学参数的因素(gmelin, 1790)
Epizootology Immunobiology Pharmacology Sanitation Pub Date : 2022-05-18 DOI: 10.47612/2224-168x-2022-1-43-48
A. M. Saroka
{"title":"FACTORS INFLUENCING THE BIOLOGICAL AND MORPHOMETRIC PARAMETERS OF HETERAKIS GALLINARUM EGGS (GMELIN, 1790)","authors":"A. M. Saroka","doi":"10.47612/2224-168x-2022-1-43-48","DOIUrl":"https://doi.org/10.47612/2224-168x-2022-1-43-48","url":null,"abstract":"The article presents the results of the research on the peculiarities of embryo and morphometric development \u0000of the eggs isolated from the gonads of female nematodes Heterakis gallinarum that parasitize turkeys and hens at different \u0000intensities of invasion in case of mono-and mixed forms of helminth infections. It was established that the eggs of H. gallinarum became invasive at 30 °C for 7 days, their survival rate in the laboratory conditions was: 80,67±2,08 % (turkeys), 84±3 % (chickens). The morphometric parameters of eggs excreted by mature H. gallinarum females are objectively influenced by such factors as the stage of biological cycle, the intensity of invasion, the type of host.","PeriodicalId":232446,"journal":{"name":"Epizootology Immunobiology Pharmacology Sanitation","volume":"67 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114034468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
STUDY OF THE TOXICITY OF AN ANTIPROTOZOIC VETERINARY PREPARATION BASED ON A DERIVATIVE OF HINAZOLINONE 以噻唑啉酮衍生物为基础的抗原生动物兽用制剂的毒性研究
Epizootology Immunobiology Pharmacology Sanitation Pub Date : 2022-05-18 DOI: 10.47612/2224-168x-2022-1-68-74
M. Kuchinsky, T. M. Savchuk, G. M. Kuchinskaya, A. S. Bayankov
{"title":"STUDY OF THE TOXICITY OF AN ANTIPROTOZOIC VETERINARY PREPARATION BASED ON A DERIVATIVE OF HINAZOLINONE","authors":"M. Kuchinsky, T. M. Savchuk, G. M. Kuchinskaya, A. S. Bayankov","doi":"10.47612/2224-168x-2022-1-68-74","DOIUrl":"https://doi.org/10.47612/2224-168x-2022-1-68-74","url":null,"abstract":"The article presents the results of toxicological studies of a new veterinary drug intended as a therapeutic and prophylactic agent for cryptosporidiosis of calves. It is established that the test sample belongs to hazard class 4 (low-hazard substances).","PeriodicalId":232446,"journal":{"name":"Epizootology Immunobiology Pharmacology Sanitation","volume":"84 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131053300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
LATENT TUBERCULOSIS INFECTION IN CATTLE 牛的潜伏结核感染
Epizootology Immunobiology Pharmacology Sanitation Pub Date : 2022-05-18 DOI: 10.47612/2224-168x-2022-1-11-21
A. Lysenko, A. E. Vysotsky, A. N. Pritychenko, M. V. Kuchvalski, E. Yakobson, E. Krasnikova
{"title":"LATENT TUBERCULOSIS INFECTION IN CATTLE","authors":"A. Lysenko, A. E. Vysotsky, A. N. Pritychenko, M. V. Kuchvalski, E. Yakobson, E. Krasnikova","doi":"10.47612/2224-168x-2022-1-11-21","DOIUrl":"https://doi.org/10.47612/2224-168x-2022-1-11-21","url":null,"abstract":"The genome of mycobacterium tuberculosis (MBT) was detected in the milk of cows from conditionally tuberculosis-free herds that did not react and gave an uncertain reaction to tuberculin in 53.3 % 66.7 % of cases, specific antibodies in 40.7 % and 57.1 %, MBT antigens in 25 % and 22.2 % of samples, and non-acid-fast forms of MBT were detected from 92.3 % and 66.7 % of the studied milk samples heated at 98 oC. Taking into account the absence of signs \u0000of active disease, this indicated a latent tuberculosis infection in some cows that did not respond to tuberculin and that \u0000the tuberculin test did not reflect the real situation in the herd. To eliminate tuberculosis infection, it is necessary to use alternative methods for determining direct and indirect markers of tuberculosis infection and the development of a new \u0000system of anti-tuberculosis measures that take into account the high probability of transmission of modified forms of MBT through the placenta and with milk.","PeriodicalId":232446,"journal":{"name":"Epizootology Immunobiology Pharmacology Sanitation","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123579989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MOLECULAR GENETIC DETECTION OF ACTINOBACILLUS PLEUROPNEUMONIAE AND HAEMOPHILUS PARASUIS 胸膜肺炎放线杆菌和副猪嗜血杆菌的分子遗传学检测
Epizootology Immunobiology Pharmacology Sanitation Pub Date : 2022-05-18 DOI: 10.47612/2224-168x-2022-1-61-67
M. M. Mistejko, E. Krasnikova, O. V. Malchik, A. S. Andrusevich
{"title":"MOLECULAR GENETIC DETECTION OF ACTINOBACILLUS PLEUROPNEUMONIAE AND HAEMOPHILUS PARASUIS","authors":"M. M. Mistejko, E. Krasnikova, O. V. Malchik, A. S. Andrusevich","doi":"10.47612/2224-168x-2022-1-61-67","DOIUrl":"https://doi.org/10.47612/2224-168x-2022-1-61-67","url":null,"abstract":"Domestic PCR systems have been designed to detect the genomes of the pathogens of actinobacillary pleuropneumonia and hemophilic polyserositis, screening of nucleotide sequences has been carried out, specific primers have been selected, and PCR parameters have been worked out. Sensitivity, specificity and repeatability results are given.","PeriodicalId":232446,"journal":{"name":"Epizootology Immunobiology Pharmacology Sanitation","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128470001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
UNIQUE PROPERTIES OF CELL WALL DEFICIENT MYCOBACTERIA TUBERCULOSIS ISOLATED FROM ULTRAPASTEURIZED MILK 从超低温牛奶中分离的细胞壁缺陷结核分枝杆菌的独特性质
Epizootology Immunobiology Pharmacology Sanitation Pub Date : 2022-05-18 DOI: 10.47612/2224-168x-2022-1-22-30
A. Lysenko, M. V. Kuchvalski, A. N. Pritychenko, A. E. Vysotsky, E. Yakobson, E. Krasnikova
{"title":"UNIQUE PROPERTIES OF CELL WALL DEFICIENT MYCOBACTERIA TUBERCULOSIS ISOLATED FROM ULTRAPASTEURIZED \u0000MILK","authors":"A. Lysenko, M. V. Kuchvalski, A. N. Pritychenko, A. E. Vysotsky, E. Yakobson, E. Krasnikova","doi":"10.47612/2224-168x-2022-1-22-30","DOIUrl":"https://doi.org/10.47612/2224-168x-2022-1-22-30","url":null,"abstract":"The DNA of Mycobacterium tuberculosis (MBT) was found in the milk of cows that did not reacted to tuberculin. After 10 hours of warming up at 98 °С cell wall deficiency (CWD) mycobacterium tuberculosis was isolated from 50% of samples of such milk. \u0000Strains of CWD MBT withstood 5 hour warming up at 98 °С. \u0000The morphology of cells naturally changed during warming, their acid-fastness increased up to the appearance \u0000of completely acid-fast cells. Sowing of warmed suspensions on a special nutrient medium in all cases gave growth of CWD MBT which did not differ from the initial strains, which confirms the unique thermal stability of MBT and indicates that even such extreme thermal exposure does not solve the problem of biosafety of milk.","PeriodicalId":232446,"journal":{"name":"Epizootology Immunobiology Pharmacology Sanitation","volume":"114 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134096764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ON THE SANITARY CONDITION OF THE MILK TRANSFER SYSTEM OF MILKING MACHINES 浅谈挤奶机输奶系统的卫生状况
Epizootology Immunobiology Pharmacology Sanitation Pub Date : 2022-05-18 DOI: 10.47612/2224-168x-2022-1-75-81
O. A. Kazheko, M. Baranovsky, A. Musica, A. E. Vysotsky
{"title":"ON THE SANITARY CONDITION OF THE MILK TRANSFER SYSTEM OF MILKING MACHINES","authors":"O. A. Kazheko, M. Baranovsky, A. Musica, A. E. Vysotsky","doi":"10.47612/2224-168x-2022-1-75-81","DOIUrl":"https://doi.org/10.47612/2224-168x-2022-1-75-81","url":null,"abstract":"The article contains a review material on the level of microbial contamination of the milk-carrying sections of the transport system of the 2ADS-N (milk pipeline), Merry-go-round, Herringbone and Parallel milking plants. \u0000The differences in the level of bacterial contamination of the surfaces of components and parts in contact with milk, depending on the volume and structural features and types of materials used for their production, have been noted. \u0000The critical contamination limits have been identified that allow taking timely corrective actions to mitigate potentially \u0000dangerous biological risks in accordance with the HACCP principles. \u0000It is indicated on the necessity of investigation of microbial contamination of milk hoses for pumping milk from the milking parlor to the cooling tank and milk hoses for pumping milk from the cooling tank to the milk delivery truck, as well as the correction of sanitary treatment regimes for these types of products.","PeriodicalId":232446,"journal":{"name":"Epizootology Immunobiology Pharmacology Sanitation","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134194662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SYNTHESIS OF ANTIBODY-ANTIBIOTIC CONJUGATES TO COMBAT BACTERIAL INFECTIONS 合成抗细菌感染的抗体-抗生素结合物
Epizootology Immunobiology Pharmacology Sanitation Pub Date : 2022-05-18 DOI: 10.47612/2224-168x-2022-1-31-36
S. M. Yakubovsky, I. V. Nasonov, N. Knysh, I. S. Radyush
{"title":"SYNTHESIS OF ANTIBODY-ANTIBIOTIC CONJUGATES TO COMBAT BACTERIAL INFECTIONS","authors":"S. M. Yakubovsky, I. V. Nasonov, N. Knysh, I. S. Radyush","doi":"10.47612/2224-168x-2022-1-31-36","DOIUrl":"https://doi.org/10.47612/2224-168x-2022-1-31-36","url":null,"abstract":"Design of antibody-antibiotic conjugates with doxycycline immunocompetent to Escherichia coli is proposed. \u0000The egg yolk immunoglobulins from hens immunized with Escherichia coli have been conjugated with the antibiotic \u0000using the glutaraldehyde as the crosslinking reagent. The reaction of conjugation was followed spectrophotometrically \u0000and revealed the formation of distinct absorption bands in ultraviolet regions. The kinetic analysis of forming the conjugates \u0000by the Guggenheim method showed that the conjugation reaction obeyed the kinetics of the first-order reactions. \u0000The velocity constant equaled 0,77 min-1. The half-conversion period of immunoglobulins during conjugation \u0000with glutaraldehyde was about 1 min. The reaction was rapid in aqueous solution at room temperature. The molar ratio \u0000of doxycycline : antibody in the conjugate was 2:1.","PeriodicalId":232446,"journal":{"name":"Epizootology Immunobiology Pharmacology Sanitation","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131488148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
EFFECT OF CONCENTRATION OF PHOSPHOLIPIDS AND HUMIC COMPOUNDS IN DILUENT COMPOSITION ON BIOLOGICAL PARAMETERS OF BULL AND HUMAN SPERM 稀释剂中磷脂和腐殖质化合物浓度对牛和人精子生物学参数的影响
Epizootology Immunobiology Pharmacology Sanitation Pub Date : 2022-05-18 DOI: 10.47612/2224-168x-2022-1-37-42
V. Zaitseva
{"title":"EFFECT OF CONCENTRATION OF PHOSPHOLIPIDS \u0000AND HUMIC COMPOUNDS IN DILUENT COMPOSITION ON BIOLOGICAL PARAMETERS OF BULL AND HUMAN SPERM","authors":"V. Zaitseva","doi":"10.47612/2224-168x-2022-1-37-42","DOIUrl":"https://doi.org/10.47612/2224-168x-2022-1-37-42","url":null,"abstract":"In the course of the experimental work, it was found that the concentration of phospholipids and an aqueous solution of activated potassium humic substances affects the viability of bovine and human sperm. \u0000Phospholipids included in the composition of the diluent up to 10.0 and 20.0 mg, after 5 hours of incubation at a temperature of (37±1) °C, provide the survival rate of bull and human sperm at 34.4±0.86–35.5±0.86 % and 36.2±0.86–32.4±0.86 % germ cells, respectively. \u0000Diluents containing 0.0025 % aqueous solution of activated potassium humate and 10.0 and 20.0 mg of phospholipids provide the highest survival rate relative to the baseline in bovine and human spermatozoa, respectively, up to 72.9–78.8% and 73. 4–76.2 %.","PeriodicalId":232446,"journal":{"name":"Epizootology Immunobiology Pharmacology Sanitation","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125611478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
EPIZOOTOLOGY OF RABIES IN BELARUSIAN POLESSYE 白俄罗斯地区狂犬病流行病学研究
Epizootology Immunobiology Pharmacology Sanitation Pub Date : 2022-05-18 DOI: 10.47612/2224-168x-2022-1-3-10
L. S. Tsvirko, T. Naumenko, I. V. Klyatsko
{"title":"EPIZOOTOLOGY OF RABIES IN BELARUSIAN POLESSYE","authors":"L. S. Tsvirko, T. Naumenko, I. V. Klyatsko","doi":"10.47612/2224-168x-2022-1-3-10","DOIUrl":"https://doi.org/10.47612/2224-168x-2022-1-3-10","url":null,"abstract":"The article covers materials of retrospective and current analysis of epizootic situation of rabies on the territory of Belarusian Polessye. During 64-year period (1958–2021) 5,871 cases of animal diseases by rabies have been diagnosed in this territory. Rabies was established in 29 animal species. Dominant place among sick animals is occupied by fox – 44, 6 %. Domestic animals are the main source of infection of the population.","PeriodicalId":232446,"journal":{"name":"Epizootology Immunobiology Pharmacology Sanitation","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116959010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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